CLINICAL BIOCHEMISTRY
·
2025
·
IF 2.1
Chunyan Zhang,Nan Bai,Yanling Yang,Yuxuan Cheng,Xiyu He,Meng Wang,Honghui Zhou,Yaping Tian
Objectives Metabolic disorders in newborns can cause significant morbidity and mortality if not diagnosed and managed early. In this study, we developed and validated a reliable LC-MS/MS method to simultaneously quantify methylmalonic acid, methylcitric acid, malonic acid, ethylmalonic acid, and total homocysteine associated with metabolic disorders. Design and methods According to Clinical Laboratory Standards Institute (CLSI) guidelines, various analytical performances (i.e., precision, linearity, accuracy and reference intervals) were rigorously evaluated. Meanwhile, sample preparation, chromatographic separation and mass spectrometric detection and incorporating stable isotope-labeled internal standards were optimized. Furthermore, calibrators and quality controls were developed to ensure standardization and traceability. Results The coefficient of variations for precision were less than 10.0 %. Meanwhile, the results showed high accuracy (recoveries: 94.57 %–109.60 %) and robust linearity (R 2 > 0.9935) over clinically relevant ranges. The limits of detection and limits of quantification for all analytes were established, enabling sensitive detection of pathological elevations. Reference intervals for pediatric populations (ages 0–1 month and 2 months to 18 years) were determined, providing essential baselines for clinical interpretation. Conclusions The newly developed method demonstrated good analytical performance and offers a standardized, high-throughput solution for clinical laboratories to improve early diagnosis and personalized management of metabolic diseases.
研究领域:
临床化学
·
代谢组学
·
新生儿筛查
·
质谱分析
·
生物医学检验
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Stress Biology
·
2023
·Vol. 3
·期 1
·1-12
·
IF 0
Fei Yu-chen,Cheng Qin,Zhang Huan,Han Chuang,Wang Xu,Li Yan-feng,Li Shi-qian,Zhao Xiao-hu
Sclerotinia sclerotiorum is a necrotrophic plant pathogenic fungus with broad distribution and host range. Bioactive compounds derived from plant extracts have been proven to be effective in controlling S. sclerotiorum . In this study, the mycelial growth of S. sclerotiorum was effectively inhibited by maleic acid, malonic acid, and their combination at a concentration of 2 mg/mL, with respective inhibition rates of 32.5%, 9.98%, and 67.6%. The treatment of detached leaves with the two acids resulted in a decrease in lesion diameters. Interestingly, maleic acid and malonic acid decreased the number of sclerotia while simultaneously increasing their weight. The two acids also disrupted the cell structure of sclerotia, leading to sheet-like electron-thin regions. On a molecular level, maleic acid reduced oxalic acid secretion, upregulated the expression of Ss-Odc2 and downregulated CWDE10 , Ss-Bi1 and Ss-Ggt1 . Differently, malonic acid downregulated CWDE2 and Ss-Odc1 . These findings verified that maleic acid and malonic acid could effectively inhibit S. sclerotiorum , providing promising evidence for the development of an environmentally friendly biocontrol agent.
研究领域:
植物病理学
·
生物化学
✓ 已验证
DOI
CHINESE JOURNAL OF CATALYSIS
·
2019
·
IF 4.914
Yan Wan,Congcong Zheng,Xianchi Lei,Mengqi Zhuang,Jinhan Lin,Wenda Hu,Jingdong Lin,Shaolong Wan,Yong Wang
ABSTRACT Acetol is a major light oxygenate and readily produced from staged or fast pyrolysis of lignocellulose biomass. Herein we report that acetol can be selectively converted to methyl pyruvate, an important fine chemical, through oxidative esterification over Au-based catalysts. Detailed experimental studies showed that Au on amphoteric supports with appropriate strength and balanced ratio of acid and base sites can facilitate the desired oxidative-esterification pathway without accelerating undesired aldol-condensation or Cannizzaro reactions. In particular, hydroxyapatite (with a Ca/P ratio of 1.62) supported Au achieved 87% selectivity to methyl pyruvate at an acetol conversion of 62%.
研究领域:
化学工程
·
催化科学
✓ 已验证
DOI
高影响因子
TETRAHEDRON
·
2024
·
IF 2.1
Nan Wang,Yuanyuan Huang,Mengke Wang,Weichun Huang,You Zi
Herein, a new approach for the synthesis of ( Z )- N -alkenyl-2-pyridones from 2-pyridones and propiolates using phosphine catalysis under mild conditions is reported. This dual Z -diastereoselective and N -selective reaction demonstrates excellent selectivity, yielding sole ( Z )- N -alkenyl-2-pyridones. Furthermore, scale-up studies confirm its practical applicability, with consistent efficiencies observed.
研究领域:
有机化学
·
合成化学
✓ 已验证
DOI
高影响因子
Polymers
·
2021
·Vol. 13
·期 3
·365
·
IF 4.329
Yi Li,Guoyong Huang,Cong Chen,Xue-Wei Wei,Xi Dong,Wei Zhao,Hai-Mu Ye
Big spherulite structure and high crystallinity are the two main drawbacks of poly(butylene succinate) (PBS) and hinder its application. In this work, a new type of copolyester poly(butylene succinate-co-butylene acetylenedicarboxylate) (PBSAD) is synthesized. With the incorporation of acetylenedicarboxylate (AD) units into PBS chains, the crystallization temperature and crystallinity are depressed by excluding AD units to the amorphous region. In contrast, the primary nucleation capability is significantly strengthened, without changing the crystal modification or crystallization kinetics, leading to the recovery of total crystallization rate of PBSAD under the same supercooling condition. The existence of specific interaction among AD units is found to be crucial. Although it is too weak to contribute to the melt memory effect at elevated temperature, the interaction continuously strengthens as the temperature falls down, and the heterogeneous aggregation of AD units keeps growing. When the aggregating process reaches a certain extent, it will induce the formation of a significant amount of crystal nuclei. The unveiled nucleation mechanism helps to design PBS copolymer with good performance.
研究领域:
材料科学
·
高分子化学
✓ 已验证
DOI
高影响因子
Cell Communication and Signaling
·
2024
·Vol. 22
·期 1
·1-20
·
IF 8.2
Guo Junchen,Wang Shanjie,Wan Xin,Liu Xiaoxuan,Wang Zeng,Liang Chenchen,Zhang Zhenming,Wang Ye,Yan Miao,Wu Pengyan,Fang Shaohong,Yu Bo
Ferroptosis is a regulatory cell death process pivotal in myocardial ischemia–reperfusion (I/R) injury. However, the precise mechanism underlying myocardial ferroptosis remains less known. In this study, we investigated the pathophysiological mechanisms of methylmalonic acid (MMA) associated with ferroptosis activation in cardiomyocytes after I/R. We found an increase level of MMA in patients with acute myocardial injury after reperfusion and AC16 cells under hypoxia/reoxygenation (H/R) condition. MMA treatment was found to be associated with excessive oxidative stress in cardiomyocytes, leading to ferroptosis-related myocardial injury. In mice with I/R injury, MMA treatment aggravated myocardial oxidative stress and ferroptosis, which amplified the myocardial infarct size and cardiac dysfunction. Mechanistically, MMA promoted NOX2/4 expression to increase reactive oxygen species (ROS) production in cardiomyocytes, aggravating myocardial injury. Notably, the increased ROS further activated ferroptosis by inhibiting solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4) expression. In addition, MMA decreased the ectopic nuclear distribution of nuclear factor E2-related factor 2 (NRF2) by increasing the interaction between NRF2 and kelch-like ECH-associated protein 1 (KEAP1). This impeded the activation of GPX4/SLC7A11, downstream of NRF2, activating ferroptosis and aggravating myocardial cell injury. Collectively, our study indicates that MMA activates oxidative stress and ROS generation, which induces ferroptosis to exacerbate cardiomyocyte injury in an I/R model. These findings may provide a new perspective for the clinical treatment of I/R injury and warrant further investigation.
研究领域:
心脏病学
·
细胞生物学
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病理生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Xianwen Hu,Peiyu Zhao,Peng Zhao,Youcun Zhu,Yifang Zhao,Lei Dai
In recent years, deep eutectic solvents (DESs) have garnered significant attention in the field of natural product extraction due to their eco-friendliness and tunable properties. In this work, six DES systems were utilized to extract chitin-glucan complex (CGC) from Shiitake mushroom (namely Lentinula edodes var. crispum) with choline chloride (ChCl) and betaine(Bet) as hydrogen bond acceptors (HBAs) and lactic acid (LA), malonic acid (MA), and urea (U) as hydrogen bond donors (HBDs). Through systematic experiments and calculations, the ChCl/LA (CLA) system exhibited the best overall performance in extracting chitin at 80 ℃, achieving a CGC yield of 32.3 %, the highest purity of chitin reached (47.5 %), a chitin acetylation degree (DA) of 65.16 %, excellent deproteinization (6.32 % residual protein) and demineralization capabilities (0.47 % residual CaCO₃). In comparation, the CGC yield of NaOH is only 21.2 %. Multiple testing analyses have confirmed that the CGC extracted by DES can maintain the composite structure of chitin and β-glucan, and has a certain degree of thermal stability, although its crystallinity is reduced and the surface is rough. Notably, the obtained CGC did not exhibit toxicity to L929 cells, with a survival rate above 80 %, which demonstrates its potential as biomaterial. This study provides a novel strategy for the green and efficient extraction of fungal chitin and its high-value utilization.
研究领域:
天然产物提取
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绿色化学
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高分子科学
·
生物材料
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真菌细胞壁处理
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Hengye Chen,Ou Hu,Haiyan Fu,Yao Fan,Lu Xu,Qingjun Meng,Lei Zhang,Wei Lan,Chunying Wu,Siyi Tang,Yuanbin She
A fluorescence paper-based sensor method by simply using thiomalic acid modified CdTe (TMA-capped CdTe) was proposed for visual detection of silver ions (Ag + ). Red-emission TMA-capped CdTe with a large amount of carboxyl groups on the surface could be combined with Ag + by electrostatic interaction, thus leading to the fluorescence quenching. It was found that the adding of amino acids contributed to fluorescence recovery by the leaving of Ag + on the surface of TMA-capped CdTe. Moreover, the more amino group, benzene and thiol group equipped in the structure of amino acid, the stronger fluorescence restoring of CdTe could be achieved. This fluorescence spectrometry method for Ag + showed low detection of limit with the value of 13.16 nmol/L, with no response to other heavy metals, which demonstrating its high sensitivity and high selectivity for Ag + . More importantly, this paper-based sensor method combined with chemometrics could accurately identify and quantify different concentrations of Ag + in different complex matrices. This fast, economical, accurate and effective approach exhibited a new idea for the on-site detection of Ag + , and was expected to be extended to the on-site detection of Ag + in biological and environmental fields.
研究领域:
分析化学
·
环境科学
·
生物医学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Nan Hu,Meng Sun,Nan Lv,Yangyang Gao,Xiaohong Fu,Dayi Xing,Xiang Guo,Shaodong Zhai,Ruiping Zhang
Myocardial infarction (MI) is the leading cause of death worldwide. The most effective way to treat myocardial infarction is to rescue ischemic cardiomyocytes. After an ischemic event, the overproduction of reactive oxygen species (ROS) is a key driver of myocardial injury. The produced ROS affects mitochondrial function and induces apoptosis in cardiomyocytes. This was accomplished by constructing platelet-membrane-encapsulated ROS-responsive drug-releasing nanoparticles (PMN@NIC-MalNPs) to deliver malonate and niclosamide (NIC). The results revealed that PMN@NIC-MalNPs degraded and released malonate and niclosamide in a high-level ROS microenvironment, effectively reducing the oxidative stress and apoptosis rate. By enhancing basal mitochondrial oxygen consumption rate (OCR), adenosine triphosphate (ATP) production, and spare respiratory capacity (SRC) in vitro, reduced the oxidative stress levels and restored mitochondrial function. In vivo studies revealed that the PMN@NIC-MalNPs improved cardiac dysfunction, inhibited succinate dehydrogenase (SDH) activity, increased ATP production, and reduced the myocardial infarct size in myocardial infarction model mice. Further, transcriptome analysis and Western blot revealed that PMN@NIC-MalNPs prevented apoptosis by activating the expressions of the signal transducer and activator of transcription 3 (STAT3) and Bcl-2, and inhibiting the expression of Bax. Thus, this study provides a novel therapeutic solution for treating myocardial infarction and predicting the viability of an antioxidant and antiapoptotic therapeutic solution in the treatment of myocardial injury.
研究领域:
心脏病学
·
药学
·
纳米技术
·
分子生物学
·
治疗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2015
·
IF 0.754
Liang HAO,Da-Peng WU,Ya-Feng GUAN
The method of micro-pressurized liquid extraction (μ-PLE) coupled with gas chromatography-mass spectrometer (GC-MS) for analysis of 4 dicarboxylic acids (DCAs, including malonic acid, succinic acid, glutaric acid, and adipic acid) in fine atmospheric particles was developed. PM 2.5 filter sample was extracted by μ-PLE at a pressure of 8 MPa and temperature of 80 °C using methanol as extraction solution. After two static 5-min extractions and a rinsing step with 100 μL methanol, about 500 μL of extraction solution was obtained. The extraction solution was then blown to dry with high purity nitrogen and submitted to a silylation reaction at 60 °C for 30 min. After internal standard (IS) was added, the derivatization solution was directly injected for GC-MS analysis. For quantification of 4 normally DCAs in fine atmospheric particles, the calibration curve was obtained with a good linear relationship in the range of 0.2–20 ng μL −1 . The limits of quantification (LOQs, S/N = 10) were in the range of 0.15–0.47 ng m −3 . The relative recoveries were between 92% and 122% with the RSDs between 2.6% and 11.8% for spiked filter samples. The method developed here was used to determine malonic acid, succinic acid, glutaric acid, and adipic acid in real PM 2.5 samples collected from Xinghai Bay of Dalian city, and the results were determined to be 64.6, 50.0, 41.8 and 20.2 ng m −3 , respectively.
研究领域:
分析化学
·
环境科学
·
大气化学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Deng Pan,Gaoyuan Ren,Jingwei Zhang,Li Wang,Shudong Wang
The effects of dicarboxylic acids (oxalic acid, malonic acid and succinic acid) on the H 2 O 2 stability during tungsten (W) chemical mechanical planarization (CMP) process were studied for the first time. The dicarboxylic acids can coordinate with Fe 3+ in the slurry and impact the reaction process by blocking the interaction between H 2 O 2 molecule and Fe 3+ , decreasing the Fe-complex redox potential and promoting the reduction of Fe 3+ to Fe 2+ . Among the three dicarboxylic acids, oxalic acid shows the superior coordination ability with Fe 3+ , resulting in a significant improvement of H 2 O 2 stability in the slurry. Appropriate amount of oxalic acid can improve the wafer surface quality (Ra=1.435 nm) while keeping the high material removal rate (MRR = 917 Å/min) at the same time. An increasing amount of dicarboxylic acids can result in a high degree of coordination between Fe 3+ and dicarboxylic acid in the slurry. The results also indicate that the main reaction path from Fe 3+ to Fe 2+ in this system is the reaction between Fe 3+ and H 2 O 2 . Meanwhile, the MRR keeps almost unchanged after one-week storage with the 2:1 mol ratio of oxalic acid to Fe 3+ .
研究领域:
材料科学
·
化学工程
·
半导体制造
✓ 已验证
产品关联
DOI
高影响因子
Energy Storage Materials
·
2025
·
IF 20.2
Xingyu Hu,Hui Shi,Yilei Zheng,Penghui Shao,Liming Yang,Xubiao Luo
The sustainable recovery of valuable metals from end-of-life lithium-ion batteries (LIBs) has become essential for relieving the supply-demand contradiction of key resources. However, the high similarity in physicochemical properties of transition metals poses a fundamental challenge for the selective recycling. Here we proposed a facile strategy for defining the demarcation line of precipitation/dissolution behavior for precise Ni/Co separation. A malonic acid-based deep eutectic solvent (DES) is developed, emphasizing the H 2 O-mediated competitive coordination and differentiated solubility. We discovered the distinctive formation of [Ni(H 2 O) 6 ] 2+ and [CoCl 4 ] 2- in the DES-20 % H 2 O system. Furthermore, the K sp differences of the Ni-complex and Co-complex in the C 3 H 2 O 4 2- -rich environment was effectively magnified. These significant differences synergistically enable the generation of C 3 H 2 NiO 4 ·2H 2 O precipitation (∼ 99.9 % purity), achieving a remarkable Ni/Co separation factor of 125, which guarantees sequential separation of all metals. This universal methodology along with in-depth mechanism understanding provides a guideline toward value-added recovery of LIBs.
研究领域:
材料科学
·
化学工程
·
环境科学
·
电化学
·
可持续技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）