FOOD CHEMISTRY
·
2021
·
IF 7.514
Lukai Ma,Qiuxuan He,Yuanxin Qiu,Huifan Liu,Jihong Wu,Guoqin Liu,Charles Brennan,Margaret Anne Brennan,Lixue Zhu
Vegetable oils are increasingly replacing animal fats in diets, but malondialdehyde (MDA), a peroxidation product of these oils, has been regarded as toxic; this necessitated investigation of MDA formation during consumption. This study investigated MDA formation in four vegetable oils during frying French fries (FF) and fried chicken breast meat (FCBM) at 180 °C for 7 h. Results showed that MDA contents were lower in oils used for frying foods than in control oils, mainly because MDA was incorporated into the foods. MDA content was lower in FF, but higher in FCBM, due to the different food components. Model oil and food system analyses yielded similar results. MDA bound the hydrophobic helical structure in starch-based FF, but was exhibited greater reactivity with nucleophilic groups in protein-based FCBM, resulting in stronger interaction with FCBM than with FF. Our results indicated the existence of distinct mechanisms underlying MDA migration in different food matrixes.
研究领域:
食品科学
·
毒理学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2020
·Vol. 10
·期 1
·1-7
·
IF 3.998
Dong Xiuli,Tang Jiayuan,Chen Xiangming
An excellent pre-column fluorescent derivatization reagent N-acetylhydrazine acridone for the quantitative determination of malondialdehyde was synthesized. Malondialdehyde was derivatized at 80 °C for 30 min in the presence of trichloroacetic acid. The separation of the derivative was performed on an Agilent ZORBAX SB-C18 column in conjunction with gradient elution. The excitation and emission wavelengths were 370 nm and 420 nm, respectively. The developed method demonstrated good linear relationship in the range of 0.02 pmol to 2.5 pmol (r = 0.9998). The calculated limit of detection and limit of quantification were 2.5 fmol and 8.3 fmol, respectively. The analytical precisions of the method were in the range of 1.36–2.27% (intra-day) and 2.36–3.92% (inter-day) respectively. The method was sensitive, specific and simple. It was successfully implemented to analysis the malondialdehyde in rat prostate.
研究领域:
分析化学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Meiji Liao,Yanyun Zhao,Yuanrong Xu,Chuting Gong,Shunshan Jiao
Hot air-assisted radio frequency (HA-RF) heating has great potential in roasting of cashew nut kernels with high roasting efficacy. This study further investigated HA-RF roasting effects on nutritional quality , malondialdehyde (MDA) and aroma compounds of cashew nut kernels. After HA-RF roasting, crude fiber content of kernels increased from 2.49 g/100 g to 3.18 g/100 g while ash and protein content did not change significantly ( p > 0.05). In raw and roasted kernels, 14 fatty acids were detected including 7 saturated fatty acids and 7 unsaturated fatty acids . Fatty acid composition and individual amino acid content were not affected by HA-RF or conventional hot air (HA) roasting. MDA content increased from 0.36 mg/kg to 0.48 mg/kg and 0.64 mg/kg after HA-RF roasting and HA roasting. The types of aroma compounds of cashew nut kernels roasted by HA-RF and HA were similar, but HA-RFK had lower furan content and higher ketone and terpene contents. This study indicated HA-RF roasting was comparable or even better than HA roasting in improving nutritional quality and flavor.
研究领域:
食品科学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2022
·Vol. 11
·期 9
·1176
·
IF 5.561
Wenjie Wang,Zhiwen Zhang,Xiaoying Liu,Xiaoji Cao,Lianzhu Wang,Yuting Ding,Xuxia Zhou
Malondialdehyde (MDA) is one of the representative end products under lipid peroxidation, indicating the degree of lipid oxidation in foods. However, compounds in pickled products, especially the nitrite in salted lean pork can react with MDA under the acidic condition, leads to the loss of MDA and an underestimation on lipid oxidation through the conventional assay. In this study, the quantification for MDA in the sample containing sodium nitrite were found lacking accuracy by the thiobarbituric acid (TBA) assay and chromatography assay based on alkaline hydrolysis as the reaction between them were difficult to be completely inhibited. Among other trials, the improvement GC-MS analysis utilizing deuterium substituted MDA (MDA-d2) as an internal standard and applying perfluorophenylhydrazine (PFPH) as a derivative reagent can reduce the deviations from the presence of nitrite in the salted lean pork meat and the recovery is between 93.9% and 98.4% and coefficient of variation for the intermediate precision is between 1.1 and 3.5% using the method. The advanced gas chromatograph mass spectrometer (GC-MS) assay also has a very low detection limit (0.25 ng/mL) with both hydrolysis types.Keywords:malondialdehyde;lipid peroxidation;pickled products;nitrite;GC-MS
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Zhigang Ke,Yiwen Bai,Yuqing Yi,Yicheng Ding,Wenjie Wang,Shulai Liu,Xuxia Zhou,Yuting Ding
An interesting phenomenon that plasma-activated water (PAW) treatment reduced the malonaldehyde (MDA) content in muscle foods was observed in both previous reports and the present study. However, the mechanism remains unclear. To clarify the theoretical basis of this phenomenon, the main reactive components in PAW were determined, and the changes in the fatty acid profile in tuna muscle after PAW treatment were analyzed. The results showed that the MDA content reduction upon PAW treatment was not due to the inhibition of lipid oxidation. To mimic the possible reaction of the components in PAW with MDA, individual hydrogen peroxide, nitrite, and nitrate or their mixture solution were added into MDA standard and tuna muscle. The results showed that the reaction of nitrite in PAW with MDA occurred during its measurement processes caused its reduction. The results in this work fully explained why PAW treatment reduced the MDA content in muscle foods.
研究领域:
食品科学
·
化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELLULOSE
·
2024
·Vol. 31
·期 6
·3717-3728
·
IF 4.9
Wang Wenxi,Han Sirui,Ren Junjie,Xiao Xiufeng,Chen Jingbo,You Ruiyun,Zhang Guifeng,Lu Yudong
A two-dimensional (2D) substrate composed of nanocellulose-gold nanostars (S-CNF/Au NSs) was prepared, which exhibited remarkable homogeneity and stability. P-aminothiophenol (PATP) was modified on the S-CNF/Au NSs substrate by Au–S bonds. The amino group of PATP was utilized to react with the aldehyde group of malondialdehyde (MDA) in a Schiff base reaction, resulting in a new Raman peak at 1655 cm −1 attributed to C = N vibration. Since the intensity of the Raman peak at 1078 cm −1 was hardly affected under acidic conditions, the ratio of the characteristic peak of C = N (1655 cm −1 ) to the peak intensity at 1078 cm −1 was used as a characterization of the MDA concentration. The precision of signal intensity was further corrected to improve confidence of the results. The developed SERS sensing system displayed satisfactory sensitivity with a linear detection range of 1 × 10 –9 ~ 2.5 × 10 –2 mM and a limit of detection (LOD) of 1.29 × 10 –11 mM. The S-CNF/Au NSs 2D substrate was able to sensitively differentiate serum from gastric cancer patients and healthy individuals by SERS analysis ( p < 0.0001), which was superior to colorimetric analysis ( p < 0.01). This result suggests that the proposed S-CNF/Au NSs 2D substrate has potential for MDA detection and can be used for early diagnosis of gastric cancer.
研究领域:
医学诊断
·
生物化学
·
材料科学
✓ 已验证
DOI
高影响因子
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
·
2017
·Vol. 100
·期 2
·208-215
·
IF 1.412
Xiang Qingqing,Xu Bofan,Ding Yilun,Liu Xiaoyi,Zhou Ying,Ahmad Farooq
The widespread contamination and persistence of the herbicide butachlor in the environment resulted in the exposure of non-target organisms. The present study investigated the toxicity effect of butachlor (1–15 µmol/L) and the protective effect of vitamin C (VC) against butachlor-induced toxicity in zebrafish. It was found that butachlor significantly increased the mortality and malformation rates in a dose-dependent manner, which caused elevation in reactive oxygen species (ROS) and malondialdehyde (MDA) after 72 h exposure. Compared with butachlor treatment group, the protective effect of VC against butachlor-induced toxicity were observed after adding 40, 80 mg/L VC respectively. VC significantly decreased the mortality, malformation rates, ROS, MDA, and normalized antioxidant enzymes activities of zebrafish after 72 h exposure. The result shows VC has mitigative effect on butachlor-induced toxicity and it can be used as an effective antioxidant in aquaculture.
研究领域:
环境毒理学
·
水产养殖科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Zefu Wang,Zhifei He,Dong Zhang,Xiaosi Chen,Hongjun Li
This study aimed to assess the protective effects of the main components of Zanthoxylum bungeanum Maxim. essential oil (ZBMEO) on the characteristics of rabbit meat myofibrillar protein (MP) under MDA (malondialdehyde) induced oxidation. The MP suspensions were mixed with various concentrations (5 mM, 25 mM and 50 mM) of linalool, limonene, sabinene under MDA-induced oxidation. The retained MDA contents were determined, and the thermal stability and structure of rabbit meat myofibrillar protein were analyzed. Linalool, limonene and sabinene can prevent MDA from binding to MP effectively. The addition of linalool, limonene and sabinene led to the increase in the sulfhydryl and free amine contents, and the decrease in the carbonyl content and surface hydrophobicity compared to control MP. Also, the addition had protective effects on maintaining the secondary structure and morphology of MP under MDA-induced oxidation. The Differential scanning calorimetry (DSC) and SDS-PAGE analysis indicated that linalool, limonene, sabinene had an obvious protective effect on MHC and actin. The above results suggest that linalool, limonene and sabinene could reduce the oxidative damage of MDA to MP, which might have the potential in protecting the structure and physicochemical properties of the protein in foods .
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2013
·
IF 2.203
R.-J. Qu,X.-H. Wang,M.-B. Feng,Y. Li,H.-X. Liu,L.-S. Wang,Z.-Y. Wang
This study investigated the effect of pH on cadmium toxicity to three aquatic organisms: Photobacterium phosphoreum , Daphnia magna and Carassius auratus . The acute toxicity of Cd 2+ to P. phosphoreum and D. magna at five pH values (5.0, 6.0, 7.0, 8.0, and 9.0) was assessed by calculating EC 50 values. We determined that Cd 2+ was least toxic under acidic conditions, and D. magna was more sensitive to the toxicity of Cd than P. phosphoreum . To evaluate Cd 2+ -induced hepatic oxidative stress in C. auratus at three pH levels (5.0, 7.25, 9.0), the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase), the level of glutathione and the malondialdehyde content in the liver were measured. Oxidative damage was observed after 7 d Cd exposure at pH 9.0. An important finding of the current research was that Cd 2+ was generally more toxic to the three test organisms in alkaline environments than in acidic environments.
研究领域:
环境科学
·
水生毒理学
·
生态毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2013
·
IF 2.906
Nannan Chen,Qiangzhong Zhao,Weizheng Sun,Mouming Zhao
Soy protein isolate (SPI) was modified by lipid peroxidation product malondialdehyde (MDA), and the in vitro digestibility of modified SPI was investigated. Results indicated that incubation with increasing MDA concentration resulted in significant carbonyl group generation and loss of free amino groups of SPI. Fluorescence loss of natural tryptophan and formation of Schiff base were observed. Noncovalent interaction between molecules was enhanced and became the main force that led to the solubility reduction of MDA-modified SPI. Differential scanning calorimetry (DSC) indicated that SPI had higher thermal stability and lower total calorimetric enthalpy after MDA pretreatment. Electrophoresis showed that β-conglycinin was more sensitive to MDA modification. In vitro digestion indicated that MDA could induce non-disulfide covalent polymer of SPI, which could not be digested by pepsin and pancreatin. β subunits of β-conglycinin became more resistant to digestion with increasing MDA concentration. Evaluation of the free amino acid profile in the digests indicated that MDA-modified SPI had deteriorating nutritive quality.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Yazhou Ji,Ruican Wang,Yuanyifei Wang,Dongfei Tan,Yaya Wang,Yuekun Wu,Haoxin Cui,Yan Zhang,Shuo Wang
Thermally processed lipid- and protein-rich foods have sparked widespread concern since they may degrade food nutrition and even risk food safety. This study investigated soy protein isolate (SPI) alterations of digestibility and structure, as well as the formation of potentially hazardous chemicals, i.e., advanced lipoxidation end products (ALEs), after interacting with malondialdehyde (MDA, a lipid oxidation product) under high-temperature cooking conditions (100–180 °C, up to 60 min). In-vitro protein digestion of the SPI-MDA mixtures suggested that their room-temperature interactions damaged SPI digestibility, and increasing the temperature and the duration of the thermal treatment exacerbated the adverse effects. Protein oxidation, covalent aggregation of subunits, and changes in secondary and tertiary structures were revealed using thiol quantification, gel electrophoresis, fluorescence spectroscopy, and circular dichroism (CD) spectra, which could explain reduced protein digestibility. High-resolution mass spectrometry (HRMS) identified seven non-crosslinked ALEs and two crosslinked ALEs. Increased MDA concentrations promoted the generation of ALEs. Moreover, the acrolein-derived ALEs with reactive carbonyl groups were prone to further reacting into crosslinked ALEs, potentially responsible for the subunit aggregation.
研究领域:
食品科学
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2019
·
IF 5.108
Yongming Wu,Jing Huang,Mi Deng,Yuanxiang Jin,Huilin Yang,Yu Liu,Qinyan Cao,Jan A. Mennigen,Wenqing Tu
6:2 chlorinated polyfluorinated ether sulfonate (F-53B), a Chinese PFOS alternative, has recently been identified in the aquatic environment at concentrations similar to or higher than perfluorooctane sulfonate (PFOS). Although previous studies have shown that F-53B can trigger oxidative stress in fish, the underlying molecular mechanism is still largely unknown. In this study, zebrafish embryos were exposed to various concentrations of F-53B (0, 0.5, 20 and 200 μg/L) for 5 d to investigate oxidative stress responses and possible molecular mechanisms of action. Our results showed that F-53B accumulated in a concentration-dependent manner in zebrafish larvae. The contents of malondialdehyde (MDA) and reduced glutathione (GSH), as well as the activities, mRNA and protein levels of most of antioxidant enzyme genes involved in the phosphatidylinositol 3-kinase (PI3K)/Akt/Nrf2-ARE pathway were significantly reduced. Further in silico study indicated that F-53B binds tightly to PI3K, which may be related to the inhibition of Nrf2-regulated antioxidant functions by F-53B as a PI3K inhibitor. Combining in vivo and in silico studies, we elucidated the effects of F-53B on antioxidant system of zebrafish through the PI3K/Akt/Nrf2-ARE pathway, which increases our understanding of the molecular mechanism of F-53B on antioxidant responses in fish.
研究领域:
环境科学
·
毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）