PROCESS BIOCHEMISTRY
·
2021
·
IF 3.757
Yujie Su,Yuzhen Wang,Cheng Lu,Luping Gu,Cuihua Chang,Junhua Li,David Julian McClements,Yanjun Yang
Individuals suffering from phenylketonuria require foods containing low levels of phenylalanine (Phe). In this research, we therefore developed a two-step enzymatic hydrolysis method followed by activated carbon treatment to remove Phe from egg white powder (EWP). Nine proteases were tested for their ability to expose or release Phe residues from the egg proteins, while nine kinds of activated carbon were tested as an adsorptive medium for removing Phe residues. Our results showed that trypsin combined with flavourzyme released the most Phe residues, and No.4 activated carbon was most effective at removing Phe residues. The percentage of Phe removal varied from 91.71–97.78% and the final mass yield from 230.08–342.45 g low Phe peptide (LPP) / Kg of EWP. The LPP mixtures obtained mainly containing amino acids and oligopeptides (< 600 Da). The EWP-LPP mixture obtained therefore has the potential to prepare medical foods for patients with phenylketonuria.
研究领域:
食品科学
·
医学
✓ 已验证
DOI
高影响因子
ACS Omega
·
2020
·
IF 2.87
Xinming Feng,Jing Guo,Rubing Zhang,Wei Liu,Yujin Cao,Mo Xian,Huizhou Liu
An aminotransferase ω-TAEn was identified from Enhydrobacter aerosaccus. The ω-TAEn was successfully expressed in Escherichia coli and the obtained enzyme showed activity toward β-phenylalanine (β-phe) at optimal conditions. For optically pure (R)-β-phe, 50% yield was observed by kinetic resolution of racemic amino with pyruvate as the amino acceptor. To obtain (S)-β-phe, the lipase/ω-TAEn catalytic system was adopted. The ω-TAEn showed strict stereoselectivity to the amino donor. The formation of (S)-β-phe was observed using 3-aminobutyric acid as the amino donor, and (S)-β-phe was obtained by asymmetric synthesis with a yield of 82%.
研究领域:
生物化学
·
生物催化
·
酶学
·
化学生物学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Xiaoxiang Hu,Yajun Bai,Tai-Ping Fan,Xiaohui Zheng,Yujie Cai
A novel alanine dehydrogenase (ADH; EC.1.4.1.1) with high pyruvate reduced activity was isolated from Helicobacter aurati and expressed in Escherichia coli BL21 (DE3). The optimum pH of the reduction and oxidation reaction were 8.0 and 9.0, respectively, and the optimum temperature was 55 °C. With pyruvate and alanine as substrates, the specific activity of HAADH1 were 268 U·mg −1 and 26 U·mg −1 , respectively. HAADH1 had a prominent substrate specificity for alanine ( K m = 2.23 mM, k cat / K m = 8.1 s −1 ·mM −1 ). In the reduction reaction, HAADH1 showed the highest substrate affinity for pyruvate ( K m = 0.56 mM, k cat / K m = 364 s −1 ·mM −1 ). Compared to pyruvate, oxaloacetic acid, 2-ketobutyric acid, 3-fluoropyruvate, α-ketoglutaric acids, glyoxylic acid showed a residual activity of 93.30%, 8.93%, 5.62%, 2.57%, 2.51%, respectively. Phylogenetic tree analysis showed that this is a new type of ADH which have a low sequence similarity to available ADH reported in references. 3-Fluoropyruvate was effectively reduced to 3-fluoro-L-alanine by whole-cell catalysis.
研究领域:
生物化学
·
微生物学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 19
·11967-11973
·
IF 4.036
Yafeng Zhang,Huizhen Wang,Hu Yu,Xiaoxia Sun
Phenylalanine an essential aromatic amino acid for humans and animals, cannot be synthesized by humans and animals on their own. However, it synthesizes important neurotransmitters and hormones in the body and is involved in gluconeogenesis and lipid metabolism. Moreover, the two opposite configurations of phenylalanine have different activities. For example, L-phenylalanine is a biologically active optical isomer involved in crucial biological processes, the lack of which will lead to intellectual disability, while D-phenylalanine only acts as a chiral intermediate. In this research, an H8-BINOL chiral fluorescent sensor modified with 1,2,3-triazole was synthesized in high yield (95%) by nucleophilic substitution and click reaction. The chiral fluorescent sensor showed high enantioselectivity toward phenylalanine. L-Phenylalanine enhanced the fluorescence response of the probe significantly, while D-phenylalanine had no obvious fluorescence response change. The enantioselective fluorescence enhancement ratio [ef = (IL − I0)/(ID − I0), where I0 is the fluorescence of the sensor without amino acids] for the highest fluorescence intensity at 20.0 equivalents of amino acids was 104.48. In this way, the probe could be used to identify and differentiate different configurations of phenylalanine.
研究领域:
生物化学
·
分析化学
✓ 已验证
DOI
高影响因子
Science Advances
·
2023
·Vol. 9
·期 22
·
IF 13.6
Jing Yu,Ruilin Xie,Mengyuan Zhang,Kaixiang Shen,Yuxuan Yang,Xiaodan Zhao,Xin Zhang,Yanfeng Zhang,Yilong Cheng
Development of underwater adhesives with instant and robust adhesion to diverse substrates remains challenging. A strategy taking the structural advantage of phenylalanine derivative, N-acryloyl phenylalanine (APA), is proposed to facilely prepare a series of underwater polymeric glue-type adhesives (UPGAs) through one-pot radical polymerization with commonly used hydrophilic vinyl monomers. The adjacent phenyl and carboxyl groups in APA realize the synergy between interfacial interactions and cohesion strength, by which the UPGAs could achieve instant (~5 seconds) and robust wet tissue adhesion strength (173 kilopascal). The polymers with varied hydrophobicity and substitutional groups as well as carboxyl and phenyl groups in separated components are designed to investigate the underwater adhesion mechanism. The universality of APA for the construction of UPGAs is also verified by the copolymerization with different hydrophilic monomers, and the applications of the UPGAs have been validated in diverse hemorrhage models and distinct substrates. Our work may give a promising solution to design potent underwater adhesives.
研究领域:
材料科学
·
生物医学工程
·
高分子化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Chenmin Zheng,Wensi Huang,Yuping Zou,Wanping Huang,Guoguang Zhang,Peng Fei
In this paper, α-alanine and β-alanine were used as modifiers to prepare α-alanine amidated pectin (α-AP) and β-alanine amidated pectin (β-AP) through enzymatic method. The effects of alanine and its isomer on the amidation degree and physicochemical properties of pectin were studied. Fourier transform infrared spectrum, proton nuclear magnetic resonance analysis, X-ray photoelectron spectrum and elemental analysis indicated that the amino groups from α-C and β-C could be aminated with the carboxyl group of pectin to form the corresponding aminated pectin. The alanine grafting ratio of α-AP and β-AP were 21.99% and 18.92%, respectively. The results showed that the dynamic viscosity of β-AP was significantly higher than that of α-AP due to the influence of amino acid side chain. However, due to the higher alanine grafting ratio of α-AP, the strength of hydrogel prepared by α-AP was higher than that of β-AP, and it also has the highest elastic modulus and swelling capacity. In addition, pectin, as a carbohydrate-based fat substitute, has been widely used in the field of food to simulate the smooth and delicate taste of fat. Compared with High methoxy pectin (HMP) and β-AP, α-AP had better colloid stability and smaller hydrogel particles in the composite system. The results provide references for the application of amidated pectin in low-fat food.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Xinli Wang,Jiahe Chen,Hui Xu,Yufei Fan,Xue Wang,Meng Zhang,Yue Liu,Binxiao Li,Jing Liu,Hong Zhou
Chiral molecules are abundant in nature. Phenylketonuria (PKU) is caused by the abnormal transformation of chiral molecules L-phenylalanine (L-Phe) in the human blood , which can cause irreversible harm to the human body. In this work, we documented an electrochemiluminescent (ECL) dual-mode sensor platform based on molecularly imprinted polymer (MIP) modified closed bipolar electrodes for high sensitivity detection of L-Phe and D-phenylalanine (D-Phe). In the anode chamber of a bipolar electrode modified with phenylalanine imprinting, Ru (bpy) 3 2+ underwent a redox reaction to produce a chemiluminescence response under the stimulation of a driving voltage . At the same time, the reduction of the cathode film of the bipolar electrode was promoted, and the color changed from dark blue to nearly white. Thus, the dual-mode detection of target molecules is realized. The detection range of the sensor for phenylalanine reached 0.01–10,000 nM, and the detection limits of L-Phe and D-Phe were 3.9 pM and 4.6 pM (S/N = 3), respectively. This dual-mode system achieved high stability and high specificity, and also successfully realized the detection of actual samples, which is expected to achieve future clinical applications.
研究领域:
分析化学
·
生物医学工程
·
传感器技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Advanced Research
·
2024
·
IF 11.4
Kai Lin,Lei Yue,Longyu Yuan,Kui Kang,Yibing Zhang,Rui Pang,Wenqing Zhang
Introduction During the adaptation to host plant resistance, herbivorous insects faced the challenge of overcoming plant defenses while ensuring their own development and reproductive success. To achieve this, a strategic allocation of energy resources for detoxification and ecological fitness maintenance became essential. Objective This study aimed to elucidate the intricate energy allocation mechanisms involved in herbivore adaptation that are currently poorly understood. Methods The rice Oryza sativa and its monophagous pest, the brown planthopper (BPH), Nilaparvata lugens were used as a model system. An integrated analysis of metabolomes and transcriptomes from different BPH populations were conducted to identify the biomarkers. RNA interference of key genes and exogenous injection of key metabolites were performed to validate the function of biomarkers. Results We found that alanine was one of the key biomarkers of BPH adaptation to resistant rice variety IR36. We also found that alanine flow determined the adaptation of BPH to IR36 rice. The alanine aminotransferase (ALT)-mediated alanine transfer to pyruvate was necessary and sufficient for the adaptation. This pathway may be conserved, at least to some extent, in BPH adaptation to multiple rice cultivars with different resistance genes. More importantly, ALT-mediated alanine metabolism is the foundation of downstream energy resource allocation for the adaptation. The adapted BPH population exhibited a significantly higher level of energy reserves in the fat body and ovary when fed with IR36 rice, compared to the unadapted population. This rendered the elevated detoxification in the adapted BPH and their ecological fitness recovery. Conclusion Overall, our findings demonstrated the crucial role of ALT-mediated alanine metabolism in energy allocation during the adaptation to resistant rice in BPH. This will provide novel knowledge regarding the co-evolutionary mechanisms between herbivores and their host plants.
研究领域:
昆虫学
·
植物科学
·
分子生物学
·
生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Jiacheng Li,Ming Zhang,Cailing Zhang,Yin Zhang,Wenbin Chen,Hao Qu,Jian Liu,Lu Wang
As obesity rates continue to rise, there is an increasing focus on reducing obesity through exercise. People are becoming more aware of the importance of weight loss through physical activity. However, the effectiveness of exercise can vary significantly among individuals, making it challenging to evaluate its impact. Therefore, establishing a reliable method for assessing exercise effectiveness is crucial for enhancing exercise quality and reducing obesity risk. It is noteworthy that the relationship between N-lactoyl-phenylalanine (N-Lac-Phe) and energy metabolism has garnered considerable attention. In this study, we developed a N-Lac-Phe biosensor by detecting L-lactic acid (L-Lac) and L-phenylalanine (L-Phe) based on Solution-Gated Graphene Field-Effect Transistors (SGGT). Our findings showed that the L-Lac and L-Phe biosensors exhibited excellent linearity within concentration ranges of 300 pM to 300 nM for L-Lac and 3 nM–1000 nM for L-Phe, with R 2 values of 0.9934 and 0.9897, respectively. The detection accuracies for these two types of SGGT biosensors were 91.63 ± 6.97% and 99.39 ± 8.53%, respectively. Using the established N-Lac-Phe, L-Lac, and L-Phe relationship model (NLL model), we could calculate the concentration of N-Lac-Phe in the RAW264.7 culture medium based on the concentrations of L-Lac and L-Phe. The biosensors demonstrated excellent accuracy and selectivity, indicating their potential for rapidly evaluating the effectiveness of exercise.
研究领域:
生物传感器技术
·
医学检测
·
材料科学
·
代谢研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ORGANIC PROCESS RESEARCH & DEVELOPMENT
·
2022
·
IF 3.858
Jianjiong Li,Shanshan Yu,Yingang Wang,Peiyuan Yao,Qiaqing Wu,Dunming Zhu
(S)-2-Aminobutane, d-alanine, and d-homoalanine are important intermediates for the production of various active pharmaceutical ingredients and food additives. The preparation of these small chiral amine or amino acids with high water solubility still demands searching for efficient methods. In this work, we identified an ω-transaminase (ω-TA) from Sinirhodobacter hungdaonensis (ShdTA) that catalyzed the kinetic resolution of racemic 2-aminobutane at a concentration of 800 mM using pyruvate as the amino acceptor, leading to the simultaneous isolation of enantiopure (S)-2-aminobutane and d-alanine in 46% and 90% yield, respectively. In addition, (S)-2-aminobutane (98% ee) and d-homoalanine (99% ee) were isolated in 45% and 93% yield, respectively, in the kinetic resolution of racemic 2-aminobutane at a concentration of 400 mM coupled with deamination of l-threonine by threonine deaminase. We thus developed a biocatalytic process for the practical synthesis of these valuable small chiral amine and d-amino acids.
研究领域:
生物催化
·
有机化学
·
药物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2016
·
IF 2.196
Dandan Han,Yumin Liu,Xiaona Li,Teng Zhang,Zhenfang Li,Shichao Du,Mingxia Guo,Shijie Xu,Bo Yu,Baohong Hou,Junbo Gong
The solubility of calcium d -pantothenate in (water + methanol) mixtures and the effect of β -alanine on the solubility of calcium d -pantothenate in a constant mole ratio of methanol and water was measured from 263.15 K to 303.15 K by the HPLC analysis method ( P = 0.1 MPa). The solubility of calcium d -pantothenate increased with increasing temperature in all the mixtures at constant composition. Besides, it also increased with mole fraction of water in (water + methanol) mixtures at a constant temperature. Additionally, the solubility of calcium d -pantothenate was enhanced by β -alanine at a given temperature. The experimental solubility in (water + methanol) mixtures was correlated by the modified Apelblat equation, the ternary NRTL equation and Solubility-Polarity Model. The Apelblat equation, the quaternary NRTL equation and the Cohn equation were adopted to correlate the experimental solubility in the (water + methanol + β -alanine) mixtures. Worthwhile, the quaternary NRTL equation was first applied to correlating the quaternary solid-liquid equilibrium. It turned out that all the selected models give good performance on solubility prediction.
研究领域:
药物化学
·
物理化学
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2016
·
IF 0.566
Da-Peng GUO,Yong-Huan WANG,Tian-Bai XU,Yuan ZHANG,Ling-Feng Li,Jin-Kai CHEN,Xiao-Zhi WANG,Peng LI,Ji-Kui LUO
The microchip field asymmetric ion mobility spectrometry (FAIMS) technology was used for rapid analysis of phenylalanine (PHE) using the metal diffusion tube-microchip μ-FAIMS system. The test pressure was set at 0.25 MPa, and the temperature of the gas generator for the diffusion tube was at 190 °C. The ion characteristic spectra were acquired under the optimized conditions with a gas flow rate of 2000 mL min −1 , a dispersion voltage of 152.8 V and a compensation voltage of −0.62 V for phenylalanine in positive mode. Phenylalanine gas samples with different concentrations were analyzed in detail by FAIMS. The results showed that the ion intensity was linearly correlated with phenylalanine concentration in the range from 6 mg L −1 to 20 mg L −1 , with the detection limit of 5.93 mg L −1 for phenylalanine. This investigation shows the feasibility of FAIMS technique for rapid detection of phenylalanine. The FAIMS instrument is simple, and has high sensitivity and fast response, and shows great potential for rapid detection of phenylalanine.
研究领域:
分析化学
·
医学检验
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）