Biosensors-Basel
·
2023
·Vol. 13
·期 3
·309
·
IF 5.4
Jingyang Zhu,Xinru Yin,Weiyi Zhang,Meilian Chen,Dongsheng Feng,Yong Zhao,Yongheng Zhu
Chlorpyrifos (CHL), profenofos (PRO) and cypermethrin (CYP) are widely used in combination to increase crop yields. However, these three pesticides can cause serious harm to human health and do not easily degrade. In this study, a novel visible paper sensor has been prepared successfully and different colorimetric reactions were utilized to detect the three pesticides simultaneously. The sensor was constructed by grafting a zwitterionic polymer onto a cellulose filter (CF) and placing it on a glass surface modified with PDMS. The branch shape was designed to form multiple detection areas, which were modified with specific pesticides and corresponding chromogenic reagents. The as-prepared colorimetric platform exhibited high sensitivity, a short detection time, a good linear response and a low detection limit (LOD) for the three pesticides (chlorpyrifos: y = 46.801 − 1.939x, R2= 0.983, LOD = 0.235 mg/L; profenofos: y = 40.068 + 42.5x, R2= 0.988, LOD = 4.891 mg/L; cypermethrin: y = 51.993 + 1.474x, R2= 0.993, LOD = 4.053 mg/L). The comparison of the results obtained by the proposed paper sensor and those obtained by spectrophotometry further revealed the stability and reliability of the paper sensor. In particular, the color intensity of the interaction between the pesticides and coloring agents could be directly observed by the human eye. The consistency of the colorimetric/optical assay was proven in real target pesticide samples. Thus, this sensing strategy provides a portable, cost-effective, accurate and visualized paper platform, which could be suitable for application in the fruit and vegetable industry for monitoring CHL, PRO and CYP in parallel.Keywords:microfluidic paper-based analytical devices;detection;chlorpyrifos;profenofos;cypermethrin
研究领域:
分析化学
·
环境科学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Miao Chen,Xuerong Li,Qiulan Li,Jitao Li,Yaohong Gu,Peng Yang,Bo Yang,Yaling Yang
Nanozyme-based dephosphorylation reactions show potential for monitoring organophosphorus pesticides (OPs) but require efficient sensing platforms. Herein, a novel dual-mode cascaded amplification sensing strategy was established for the rapid detection of profenofos by employing metal-organic framework-based nanozymes (Ce-UiO-66/Zn) and carbon-dot-based nanozymes (Ce-CDs/MnO 2 ). Based on the high phosphohydrolase-like activity of Ce-UiO-66/Zn, profenofos could be rapidly hydrolyzed and the hydrolysis product would be further oxidized by Ce-CDs/MnO 2 with laccase-like activity, and the oxidative product was coupled with 4-aminoantipyrine to form a red product for colorimetric detection. Additionally, this cascade reaction was also used in the exploration of a smartphone sensing platform due to its obvious color change, which was used to visually analyze profenofos in different food samples and achieved ideal recovery rates. This study provides new insight into development of highly sensitive and selective biosensors, food safety and environmental on-site monitoring.
研究领域:
分析化学
·
纳米技术
·
环境监测
·
食品安全
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Tehseen Yaseen,Hongbin Pu,Da-Wen Sun
Fast sampling and multicomponent detections are important in the analysis of pesticide residues detection. In this work, surface-enhanced Raman scattering (SERS) method based on silver-coated gold nanoparticles (Au@Ag NPs) was used to simultaneously detect multi-class pesticide residues such as thiacloprid (carbamate), profenofos (organophosphate) and oxamyl (neonicotinoid) in standard solution and peach fruit. The Au@Ag NPs with 26 nm Au core size and 6 nm Ag shell thickness exhibited significant Raman enhancement, especially by the creation of hot spots through NPs aggregation induced by the connection between Au@Ag NPs and target molecules. The findings demonstrated that the characteristic wavenumber of the pesticides (thiacloprid, profenofos, and oxamyl) could be precisely identified using the SERS method. Compared with earlier studies, the current approach was rapid, inexpensive and without lengthy sample pretreatment. Moreover, the results revealed that the limit of detection (LOD) was 0.1 mg/kg for thiacloprid obtained in the peach extract with determination coefficient (R 2 ) of 0.986. Additionally, LOD for both profenofos and oxamyl was 0.01 mg/kg with a determination coefficient (R 2 ) of 0.985 and 0.988, respectively. Good recovery percentage (78.6–162.0%) showed the high SERS activity with better accuracy for the detection of the thiacloprid, profenofos, and oxamyl in peach. The results of this study could offer a promising SERS platform for simultaneous detection of other contaminants such as thiacloprid, profenofos and oxamyl in multifaceted food matrices.
研究领域:
分析化学
·
食品科学
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yusha Huang,Longcheng Gong,Chengli Xie,Wenling Qin,Min Wang,Lianzhe Hu,Zhining Xia
Herein, a biomimetic approach is developed to improve the phosphatase-like catalytic activity of a metal–organic framework (MOF) nanozyme. Specifically, biomimetic Zn sites, featuring a coordination environment similar to that of natural phosphatase, were covalently anchored into UiO-66-NH 2 nanozyme through a step-by-step modification process. Compared with pristine UiO-66-NH 2 , the resultant 66-IS-Zn exhibited a 130-fold increase in K cat . Mechanism studies suggest that the anchoring of Zn sites can only enhance the catalytic performance of MOFs that possess intrinsic phosphatase-like activity, and the enhancement of catalytic activity strongly relies on the coordination environment of Zn sites, which involves the co-coordination of N and O with Zn. Besides the excellent phosphatase-like activity, 66-IS-Zn also exhibited enhanced intrinsic fluorescence emission and high phosphodiesterase-like catalytic activity. Consequently, 66-IS-Zn was used as the sensing unit to construct a three-channel sensor array for distinguishing organophosphorus pesticides (OPs). This sensor array can successfully distinguish six OPs and allow the quantification of profenofos and phoxim. Moreover, the binary mixtures of OPs and cherry tomato samples were precisely discriminated without any overlapping, indicating the reliability and practicability of the sensor array.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Li Wang,Kang Wei,Yi Pan,Yang Wei,Zhengwu Wang,Lurong Xu,Yuanfeng Wang,Xinlin Wei
The hazardous effects of residues from organophosphorus pesticides (OPPs) on human health have prompted researchers to look for innovative adsorbents and detecting techniques. Herein, a simple and economical material Fe 3 O 4 @PCN-224 was effectively produced as a desirable adsorbent for OPPs residues adsorption by integrating magnetic polydopamine (PDA) with the zirconium-based metal-organic framework (PCN-224). Modification of magnetic cores with PCN-224 allowed a dramatic increase in surface area and porosity to enhance pesticide adsorption. Moreover, the adsorption mechanisms inferred that π-π interactions, covalent bonds, and hydrogen bonds may promote the beneficial adsorption of OPPs by Fe 3 O 4 @PCN-224. Finally, the Fe 3 O 4 @PCN-224-based HPLC method exhibited with low detection limits (0.014–0.051 μg/L), good precision (1.70 % – 3.49 %), wide linearity (1–100 μg/L, R 2 ≥ 0.9936), satisfactory recoveries (86.16 % – 106.45 %), and high enrichment factors (92–101), demonstrating its significant potential for efficient extraction, enrichment, and analysis of OPPs in complex matrix samples.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROSCOPY LETTERS
·
2025
·
IF 1.6
Jin Dangqin,Zhou Hui,Xiao Jiali,Gao Qin,Gong Aiqin
This study presents the development and validation of UV-spectrophotometry methods for the quantification of organophosphorus pesticide residues in fruits and vegetables based on solid phase extraction and the principle of enzyme inhibition of catalase. A silica gel modified with 3,3 ‘- dodecyl-1,1’ - di-1-vinylimidazolium bromide ionic liquid (Poly - (3,3 ‘- dodecane-1,1’ - bis-1-vinylimidazolium dibromide modified silica), Poly (DBVD) - Sil, is used as a solid-phase extraction adsorbent. At the same time, based on the principle of enzyme inhibition of catalase, bovine liver catalase was screened as the enzyme solution, and a rapid detection method for profenofos residue was established by solid-phase extraction UV-spectrophotometry by optimizing the reaction conditions. That CAT inhibited the oxidation of MB by H2O2, limited MB fading, and the degree of absorbance recovery was related to CAT activity. Profenofos has a significant inhibitory effect on catalase, with a decrease in absorbance values. The natural logarithm of profenofos is linearly related to the enzyme activity inhibition rate within the range of 0.001-5.0 mg/L, the linear regression equation is y = 0.0509x + 0.4341, with R2=0.9968, the detection limit (3s/k) is 3.1 × 10−4 mg/L. A standard recovery rate of 95.3% to 104.2% for profenofos. The results showed the method is simple, fast, precise, accurate, and has a low detection limit, meeting the needs of rapid detection of profenofos residues in fruits and vegetables.
研究领域:
分析化学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Lingjun Geng,Jingjing Liu,Jingcheng Huang,Haifang Wang,Peisen Li,Rui Xu,Chengqiang Li,Haowei Dong,Ibrahim A. Darwish,Yemin Guo,Xia Sun
Due to the existence of organophosphorus pesticides (OPs) in the environment and their potential hazards to ecosystems and human health, this study aimed to develop a novel adsorbent of OPs using multifunctional carriers and surface molecular imprinting technology (SMIT). SiO 2 -COOH served as a carrier, and molecularly imprinted polymers (MIPs) constituted a shell, creating a core-shell structured adsorbent. SMIT facilitated rapid and efficient binding between the target molecules and the imprinted cavities. The multifunctional SiO 2 -COOH enhanced the adsorbent's adsorption capacity, improved its mechanical stability, and strengthened the binding with the MIPs. The adsorbent demonstrated excellent specificity and reusability, achieving an adsorption capacity of 54.4 mg/g and the imprinting factor of 2.94. The relative recovery rate in actual sample detection ranged from 95.1% to 108.7%. This study provides a simple and effective method for detecting hazardous factors in environment protection and food safety fields, with significant scientific value and practical application potential.
研究领域:
环境科学
·
食品安全
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2026
·Vol. 9
·期 31
·14938-14948
·
IF 5.8
Chen Cen,Song Jiacheng,Liu Rui,Wei Jinxiu,Wang Xi,Liu Xiaohong,Li Yuye,Zhu Chengxi
Accurate and sensitive identification of pesticide residues
in
food is essential for food safety supervision and risk control. Herein,
a ratiometric electrochemical sensing platform based on a ferrocene-decorated
covalent organic framework/2D-SiO 2 /MWCNTs nanocomposite
(COFs/2D-SiO 2 /Fc/MWCNTs) was constructed for accurate quantification
of fenitrothion (FNT). The nanocomposite was synthesized via a mild
self-assembly approach, in which 2D-SiO 2 acted as a 2D
scaffold to improve the dispersion of COFs and promote the stable
immobilization of Fc. The crystalline porous COFs provided abundant
sites for FNT enrichment, the firmly immobilized Fc served as a stable
internal reference for ratiometric sensing, and the incorporated MWCNTs
effectively boosted the conductivity and electrocatalytic activity
of the nanocomposite. The obtained COFs/2D-SiO 2 /Fc/MWCNTs
nanocomposite showed favorable electrocatalytic performance toward
FNT. With the ratiometric signal I FNT /I Fc as
the readout, the sensor displayed a linear response from 0.0001 to
50 μg·mL –1 . Compared with conventional
single-signal sensors, this ratiometric method enables efficient self-calibration
against environmental interference, enhancing the accuracy and reliability
of FNT detection in food samples.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Yao He,Chunlei Lv,Fulin Li,Yichen Li,Xiangyue Tian,Xi Chen,Qiyu Yang,Juan Su,Daichen Mu
We have designed a sensor based on gold nanoparticles (AuNPs) and aptamers for two pesticides detection. Two complementary paired aptamers have been carefully designed to adsorb onto the surface of AuNPs, serving as binding elements and molecular switches for pesticides. Aptamers not only give AuNPs the ability of colorimetric detection, but also has the ability of fluorescence detection, and AuNPs can improve the stability of aptamers. Thiamethoxam (TMX) and Acetamiprid (ACE) and their corresponding aptamers (Tapt and Aapt) were selected as models. When TMX is added, Tapt can bind to it and trigger the aggregation of AuNPs which can be used as the detection signal of colorimetric detection. When ACE is added, Aapt can bind to it and produce fluorescence. ACE does not cause colorimetric reaction, and TMX does not cause fluorescence. By using both colorimetric and fluorescent detection methods, interference free detection of two targets can be achieved.
研究领域:
分析化学
·
生物传感
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of the American Chemical Society
·
2025
·
IF 15.6
Yeqing Xu,Abuduheiremu Awati,Shan Zhou,Runhao Zhang,Xin Zhang,Hui Zeng,Yaxin Guo,Yuanbo Song,Chengmin Hu,Lei Xie,Qirui Liang,Kang Liang,Lei Jiang,Dongyuan Zhao,Biao Kong
Pesticides are vital for crop and seafood production but leave persistent residues that pose risks to ecosystems and human health through bioaccumulation. The detection of pesticide residues requires highly sensitive and selective technologies. Herein, a nanochannel sensor capable of dual recognition of ionic charge and molecular conformation based on molecular imprinting technology (MIT) is presented, offering a significant improvement in selectivity and sensitivity over traditional nanopore sensors. The MIT-based nanochannels with imprinting sites tailored to pesticide molecules go beyond recognizing the molecular size and surface functional groups, enabling the detection of molecular configurations. In this research, the approach enables the detection of 10 pesticide molecules with detection limits (LODs) ranging from 12.9 to 26.9 pM, achieving two orders of magnitude lower than fluorescence-based methods. Density functional theory (DFT) and molecular dynamics (MD) simulations revealed hydrogen bonding as the dominant interaction in the imprinting process. This versatile nanochannel construction method, proposed for the first time, provides dual recognition capabilities and is expected to advance nanochannel sensing while promoting sustainable environmental development.
研究领域:
环境科学
·
生物传感技术
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Xueer Lin,Jiahui Zou,Guancheng Zhu,Linfeng Pang,Yuqi Chen,Yanyan Huang
To effectively overcome the shortcomings of traditional chromatographic and enzymatic methods for detecting organophosphorus pesticides, Cu-MOFs with inherent peroxidase (POD)-like activity were constructed for direct colorimetric detection of omethoate (OMT). Benefiting from the mesoporous and benzene ring structure, Cu-MOFs demonstrated excellent OMT enrichment capability. OMT exhibited a pronounced inhibitory effect on the POD-like activity of Cu-MOFs whereas other pesticides showed negligible interference, demonstrating the specific identification of OMT by Cu-MOFs. Experimental data and theoretical calculations indicated that OMT effectively bond to Cu-MOFs through Cu 2+ coordination and ligand binding. Owning to its enrichment, identification and catalytic abilities, OMT detection was achieved and the limit of detection (LOD) was 16 μg L −1 . Furthermore, a smartphone-assisted portable detection was established with a LOD of 79 μg L −1 . Validation experiments of cabbage samples confirmed its’ reliability for practical applications. Our system integrated the enrichment, identification, and detection of OMT within a single system, improving both sensitivity and operational convenience.
研究领域:
分析化学
·
纳米技术
·
环境科学
·
生物传感器
·
农药检测
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiutan Peng,Yuqing Xu,Weiye Zhou,Yiting Tao,Haiyan Fu,Siying Che,Yuanbin She
Evaluation of glyphosate content in tea products is a food safety topic worthy of social attention due to most glyphosate is dissolved in water as well during tea brewing. So it requires that the probe can not only realize the sensitive detection of glyphosate, but also has good water solubility to realize the practical detection of glyphosate in water-based samples such as tea infusions. To better suit the demands of practical applications, a water-soluble quinoline fluorescent ionic liquid probe (EHQCA) was synthesized for the sensitive detection of glyphosate by fluorescence “turn-off-on”. Interestingly, the introduction of the imidazolium cation module easily achieved the switchable water solubility of the fluorophore without complex group modification. According to the different competitive coordination ability, EHQCA-Cu 2+ with fluorescence-off was first constructed, and then Cu 2+ was captured by glyphosate to restore fluorescence. The probe behaved a sensitive (detection limit of 2.2 × 10 −8 M), rapid (less than 5 s) and selective response (among 20 interferences) to glyphosate. Meaningfully, satisfactory recovery (98–103 %) and low RSD (< 1.27 %) were obtained in 12 green tea infusions from different varieties and origins. Therefore, the ionic liquid-based fluorescent probes offers strong technical support for the detection of pesticides in foods such as tea, and have broad prospects in the field of food safety monitoring and evaluation.
研究领域:
分析化学
·
食品安全
·
环境监测
·
荧光光谱
·
农药检测
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）