SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Yimiao Lin,Jiafei Xie,Qingqing Xiang,Yi Liu,Pingya Wang,Yichun Wu,Ying Zhou
With the rapid increase in the use of plastic films , microplastic (MP) pollution in agricultural soils has become a global environmental problem. Propiconazole is widely used in agriculture and horticulture; however, its role in plastic film degradation remains elusive. Butylene adipate- co -terephthalate (PBAT) and polyethylene (PE) films were used to analyze the effects of propiconazole on plastic film and MP degradation. We identified the surface morphologies of PBAT and PE at different propiconazole concentrations and soil pH values, as well as the adsorption and release characteristics of heavy metals during the degradation process via scanning electron microscopy, Fourier transform infrared spectroscopy and inductively coupled plasma mass spectrometry . Propiconazole accelerated the degradation of MPs, adsorption of heavy metals (Ni and Zn), and release of Sn at low concentrations (≤40 mg/kg); however, these effects were evidently absent at a high concentration (120 mg/kg). Furthermore, MPs were more prone to degradation in acidic or alkaline soils than in neutral soil when they coexisted with propiconazole. Hence, we suggest that PBAT and PE plastic films may not be suitable for application in acidic and alkaline soils with propiconazole, because of shorter rupture time and more heavy metal adsorption. PBAT degraded faster, absorbed and released more heavy metals than PE. Under all tested conditions, the heavy metal contents in MPs gradually approached those in soil, which proves that MPs are carriers of heavy metal pollutants. These results may help in assessing the impact of MPs on soil environments and provide a theoretical basis for the standardized propiconazole and plastic film usage.
研究领域:
环境科学
·
土壤学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Physiology
·
2019
·
IF 3.201
Shentu Xuping,Wang Xiaolong,Xiao Yin,Yu Xiaoping
Yeast-like symbiotes (YLS), harbored in the abdomen fat-body cells of the rice brown planthopper (BPH), Nilaparvata lugens Stål (Hemiptera: Delphacidae), are vital to the growth and reproduction of their host. It is feasible to manipulate BPH infestation on rice by inhibiting YLS using fungicide. In this study, the fungicide propiconazole was injected into the hemolymph of BPH thorax via microinjection to investigate its effect on YLS, especially the dominant species, Hypomyces chrysospermus, and their host BPH. Propiconazole markedly reduced the total number of YLS and H. chrysospermus in BPH hemolymph and fat body, thereby leading to an obvious higher mortality and lower fecundity of BPH than the negative control (PBS, phosphate buffer solution). After microinjecting propiconazole, the survival rate of BPH nymphs at the 5th instar was significantly lower than that obtained after PBS treatment. Eight days after propiconazole microinjection, the BPH survival rate dropped to 40%, only half of BPH survival rate treated with PBS microinjection. For female adults (1-day-old), there were significant differences in the survival rates between BPHs treated with propiconazole and those treated with PBS at days 5–8. The fecundity of BPH decreased significantly by microinjecting propiconazole and averaged only 229 eggs per female, which was 20% less than that of the negative control. Furthermore, we reared BPH on the susceptible variety TN1 sprayed with propiconazole to prove the feasibility manipulating field occurrence of BPH by inhibiting YLS using fungicides. The number of YLS and H. chrysospermus in BPH obviously declined. Subsequently, the survival rate and fecundity of BPH significantly decreased after feeding on rice treated with propiconazole. Meanwhile, the propiconazole residue was detected in the hemolymph and gut of BPH by HPLC analysis within 1 day of feeding. Inhibiting YLS using fungicides was a novel and effective way to control BPH infestation.
研究领域:
昆虫学
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植物保护
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微生物学
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产品关联
DOI
PubMed
高影响因子
Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure & Risk Assessment
·
2022
·
IF 3.549
Ze-Ke Xu,Jing-Nan Meng,Yi Lei,Xing-Xing Yang,Yi-Yong Yan,Hai-Hong Liu,Hong-Tao Lei,Ting-Cai Wang,Xing Shen,Zhen-Lin Xu
The conazole fungicide propiconazole is frequently found in vegetables although usage is not allowed. To overcome the high-cost and time-consuming labour requirements of instrumental methods, we developed a simple and visual lateral flow immunoassay for the sensitive determination of propiconazole. A hapten was carefully designed to raise a monoclonal antibody against propiconazole. Bal b/c mice were immunised with the hapten-carrier protein conjugate and a specific monoclonal antibody (mAb) was produced. Based on this mAb, a sensitive immunochromatographic strip assay (ICA) was established for rapid screening of propiconazole in vegetable samples. After optimisation of analytical parameters, the ICA strip showed a detection limit of 0.13 ng g−1 and a linear range from 0.5 to 80 ng g−1 using a strip reader. The assay also can be read by the naked eye with a visual limit of detection of 80 ng g−1. The recoveries for spiked vegetable samples by ICA ranged from 85.2% to 114.9%, with a coefficient of variation less than 11.7%. The assay time is within 45 min for a single sample including the sample pre-treatment. For spiked and blind samples, the detection capability of ICA was equivalent to liquid chromatography-mass spectrometry.
研究领域:
分析化学
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食品科学
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免疫学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2025
·Vol. 48
·期 1
·e70077
·
IF 2.8
Marzieh Kavian,Milad Ghani,Jahan Bakhsh Raoof
Herein, an amino-functionalized covalent organic framework was synthesized and accommodated in the pores of porous hollow fiber. In this context, tetra (4-aminophenyl) porphyrin was synthesized for preparing the desired covalent organic framework as the extracting sorbent and employed for hollow fiber solid-phase microextraction of tebuconazole and propiconazole. With respect to the amino groups of the as-synthesized porphyrin-based covalent organic framework, the extracting device has the ability of establishing a hydrogen bond with the selected model analytes. Under the optimum condition, the linear range of the method for both analytes were estimated in the range of 0.5–200 µg L −1 (the coefficient of determination of 0.9962 for tebuconazole and 0.9990 for propiconazole). The limits of detection of the method for tebuconazole and propiconazole were calculated to 0.02 µg g −1 and 0.03 µg g −1 , respectively. The limits of quantification of the method were also estimated for two analytes equal to 0.07 and 0.08 µg g −1 , respectively. The intra- and inter-day relative standard deviations, which fell between 1.8% and 4.7%, were computed to assess the accuracy of the suggested approach. The proposed method was used for the extraction and determination of tebuconazole and propiconazole in tomato, cucumber, apple, cabbage, and carrot, which the obtained results showed the success of the method in extracting and determining of these analytes from the target samples.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xu Xu,Tongxin Sun,Xinyue Zhou,Zhuang Liu,Lei Zhang
The nanozyme-based colorimetric sensor shows promise for rapid pesticide detection but struggles with non-specific enzyme inhibition. This study developed a portable paper-based sensor for detecting the propiconazole (PC) pesticide using Fe@PCN-224 nanocubes (NCs). Characterization confirmed the successful synthesis of Fe@PCN-224 NCs, which displayed peroxidase-like activity. The specific interaction between PC's triazole ring and the Fe active site inhibited their activity, enabling selective detection with a limit of 8 × 10 −9 mol L −1 and a linear range of 0.03 × 10 −6 to 0.90 × 10 −6 mol L −1 . Kinetic studies revealed a Michaelis-Menten constants ( K m ) of 0.68 × 10 −3 mol L −1 for TMB, indicating higher affinity in Fe@PCN-224 NCs. The electron paramagnetic resonance (EPR) analysis revealed the production of •OH, 1 O 2 , O 2 • − during the catalytic reactions. By integrating smartphone technology, this portable sensor achieved recoveries from vegetable samples between 94.6 % and 109.2 %, demonstrating its potential as an accurate, cost-effective analytical tool for food safety and advancing nanozyme applications.
研究领域:
分析化学
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环境科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2024
·Vol. 38
·期 9
·e5935
·
IF 1.8
Feng Lan,Xinyu Wang,Xuejing Wang,Yinwei Ruan,Li Ding,Daliang Liu,Tongliang Zhang,Jianping Wang
Dissipative behavior and final residue levels of difenoconazole, prochloraz, propiconazole, and pyraclostrobin in figs were investigated using field trials and laboratory assays. A three-factor, three-level orthogonal test was designed to optimize the pretreatment conditions of the method. A method was established using high-performance liquid chromatography tandem mass spectrometry for the determination of difenoconazole, prochloraz, propiconazole, and pyraclostrobin residues in figs. The limit of quantification for all four targets in figs was 0.002 mg/kg. Difenoconazole, prochloraz, propiconazole, and pyraclostrobin are readily digestible pesticides in figs with half-lives of 6.4, 6.2, 4.8, and 7.9 days, respectively. Residues of difenoconazole, prochloraz, propiconazole, and pyraclostrobin in figs were below the European Union established residue levels of 0.1, 0.03, 0.01, and 0.02 mg/kg, respectively, at day 7 after application. Pyraclostrobin, propiconazole, difenoconazole, and prochloraz were applied twice at doses of 75, 125, 150, and 200 mg a.i./kg at 7-day intervals, and the residues of the four fungicides in figs were acceptable 7 days after the last application. Therefore, the safety interval can be set at 7 days for 70% difenoconazole-prochloraz wettable powder and 40% pyraclostrobin-propiconazole aqueous emulsion according to the protocol.
研究领域:
食品科学
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分析化学
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植物保护
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 39
·28976-28983
·
IF 3.9
Junqi Feng,Yanyan Zheng,Tao Luo,Fengna Xi,Hao Lai
The development of simple, rapid, and sensitive methods for detecting pesticide in environmental and food samples holds significant importance. Electrochemiluminescence (ECL) sensing platforms with high resistance to interference and contamination, and reduced consumption of ECL emitters, are highly desirable for such applications. In this work, we present an ECL sensing platform based on a graphitic carbon nitride nanosheets (CNNS) supported vertically ordered mesoporous silica film (VMSF) modified glassy carbon electrode (GCE) for the highly sensitive detection of the environmental pollutant 2,4,6-trichlorophenol (TCP) and the broad-spectrum insecticide prochloraz. Two-dimensional (2D) CNNS were synthesized by exfoliating bulk graphitic carbon nitride (g-C3N4) using concentrated sulfuric acid, serving as a novel conductive and adhesive layer for the growth of a stable VMSF on GCE via an electrochemical assistance self-assembly (EASA) method to prepare VMSF/CNNS/GCE. The electrostatic enrichment capability of VMSF nanochannels for the positively charged ECL emitter tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) realized stable and significantly enhanced ECL signals at a low concentration of Ru(bpy)32+ (10 μM). Based on the quenching effect of TCP on the ECL signal of Ru(bpy)32+, highly sensitive ECL detection of TCP was achieved by the VMSF/CNNS/GCE with a linear range from 10 nM to 0.7 mM and a low detection limit (DL) of 2.2 nM. As the metabolic end product of prochloraz is TCP, indirect ECL detection of prochloraz was also accomplished by measuring the produced TCP. Combined with anti-fouling and anti-interference abilities, as well as signal amplification of VMSF, the developed VMSF/CNNS/GCE sensor enabled the sensitive ECL detection of TCP in pond water and prochloraz in orange peel extract.
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Guiling Yang,Yanhua Wang,Jian Li,Dou Wang,Zhiwei Bao,Qiang Wang,Yuanxiang Jin
Chlorothalonil (CTL), carbendazim (CBZ), and prochloraz (PCZ) are fungicides widely used in many countries. The use of these fungicides raises concerns because they are often applied together or used in the same agricultural area. However, the toxicity of these fungicides or mixtures, especially to aquatic organisms, has received limited attention. Here, embryonic and larval zebrafish were exposed to indicated concentrations of CTL, CBZ, and PCZ and their binary (CTL+CBZ, CTL+PCZ and CBZ+PCZ) and ternary (CTL+CBZ+PCZ) mixtures for 24 h. Based on metabolomics analysis, we observed that hundreds of metabolites were altered, and glycolysis metabolism and amino acid metabolism were the two most affected pathways. Interestingly, a total of 9 and 26 metabolites changed significantly in embryos and larvae treated with all fungicides, respectively. Among these altered metabolites, 2-aminoadipic acid (2-AAA) levels increased significantly in all groups, indicating that 2-AAA potentially represents a useful biomarker for evaluating the toxicity of fungicides. Furthermore, the joint effects of CTL+PCZ on embryos and larvae, especially on amino acid metabolism, were weaker than those in other groups, but combined treatment did not influence individual fungicidal activity. Data acquired from metabolomics provided important insight for understanding the mechanism by which fungicides or their mixtures affect zebrafish.
研究领域:
环境科学
·
毒理学
·
水生生物学
·
代谢组学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Min Hu,Yu Zhang,Yan Zhang,Meng Wang,Shanying Zhang,Jin Zhang,Congying Xu,Xiaoyu Liang
Pesticides are essential for controlling plant diseases and pests in modern agriculture, yet traditional formulations often suffer from poor adhesion, rapid UV degradation, and loss due to rainwater runoff. This study introduces Pro@TA-Cu 2+ -Pal, a novel pH-responsive release system designed to enhance the performance of prochloraz, a widely used fungicide. By integrating tannic acid and copper ions onto palygorskite, this nanocarrier with metal–phenolic networks improves prochloraz wettability and adhesion on leaf surfaces while significantly enhancing UV stability and rainfastness. Under UV exposure, Pro@TA-Cu 2+ -Pal showed a 3.9-fold lower degradation rate than the commercial emulsion-in-water formulation (Pro EW). In simulated rain tests, Pro@TA-Cu 2+ -Pal exhibited superior rain-fastness, retaining >80 % of the applied fungicide on leaf surfaces, whereas Pro EW showed a marked reduction in retention. Pot trials showed that Pro@TA-Cu 2+ -Pal provided excellent control against wheat powdery mildew, outperforming Pro EW in both protective and curative treatments, with more effective and sustained disease control. Importantly, Pro@TA-Cu 2+ -Pal proved safe with no phytotoxicity observed in the crop. These findings highlight the potential of Pro@TA-Cu 2+ -Pal as an economical, eco-friendly, and sustainable solution for improving pesticide efficiency, reducing environmental impact, and supporting long-term crop protection.
研究领域:
农业化学
·
纳米技术
·
农药科学
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环境科学
·
植物病理学
✓ 已验证
DOI
高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Jiao Li,Yuan Ding,He Chen,Wanlin Sun,Yue Huang,Fengquan Liu,Minghua Wang,Xiude Hua
Propiconazole is a widely used fungicide in agricultural production, but it poses risks to human health. Here, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a monoclonal antibody (mAb) was established to detect propiconazole residues. Three haptens of propiconazole were designed and synthesized to prepare antigens. Ten mAbs were obtained using hapten B (5-(4-(2-((1H-1,2,4-triazol-1-yl)methyl)-4-propyl-1,3-dioxolan-2-yl)-3-chlorophenyl) pentanoic acid). The ic-ELISA based on the combination of mAb 2G2E12 and hapten B-ovalbumin (coating antigen) showed the highest sensitivity. After optimization, the 50% inhibition concentration, limit of detection and linear range of the ic-ELISA were 2.33 μg/L, 0.26 μg/L and 0.26–21.28 μg/L, respectively. The cross-reactivities for the analogs of propiconazole were ≤1.9%. The average recoveries of the spiked samples were 75.7%–117.0% with relative standard deviations of ≤9.4%. The results of the ic-ELISA were consistent with those of gas chromatography-mass spectrometry in the analysis of blind samples. Therefore, we provide an alternative tool for the accurate and reliable determination of propiconazole.
研究领域:
农业化学
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分析化学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
Applied Materials Today
·
2025
·
IF 6.9
Mahesh P. Bhat,Vinayak Hegde,Cheol Soo Kim,Mahaveer Kurkuri,Kyeong-Hwan Lee
Traditional pesticide formulations often suffer from limitations such as poor foliar retention, uneven distribution, low plant uptake, and inefficient utilization, necessitating the need for advanced, sustainable alternatives. In this study, a hybrid carrier system, Pec@Pro@Ti-MOF@DE, was developed to enhance the efficiency of prochloraz (Pro) against gray mold ( Botrytis cinerea ). The system integrates titanium metal-organic frameworks (Ti-MOF) anchored onto diatomaceous earth (DE), with pectin (Pec) coating serving as a responsive release barrier. The incorporation of DE significantly improved the foliar adhesion of MOF particles, enabling pesticide presence at infection sites and promoting efficient penetration and systemic translocation. The high surface area of Ti-MOF@DE (731 m 2 /g) facilitated a substantial prochloraz loading capacity of 56.5 %. The pectin coating on the Pro@Ti-MOF@DE functioned as a biological gatekeeper, releasing prochloraz in response to enzymatic pectinase activity and pH changes associated with B. cinerea infection, achieving a 91.5 % cumulative release. Foliar adhesion studies confirmed strong carrier attachment, while fluorescence microscopy revealed bidirectional translocation within plant tissues. In vitro assays demonstrated superior antifungal activity, with an EC 50 of 0.017 µg/mL, significantly lower than that of free prochloraz (0.027 µg/mL). In vivo and post-harvest evaluations further validated the system’s performance, achieving fungal control efficacy of 97.3 % in tomato leaves and 95.6 % in fruits. Therefore, Pec@Pro@Ti-MOF@DE could be used as a biocompatible carrier for site-specific pesticide delivery without compromising plant safety, which would be a sustainable approach to improve prochloraz efficacy while reducing environmental risks in modern agriculture.
研究领域:
植物科学
·
农药制剂
·
纳米技术
·
真菌病理学
·
控释系统
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Jing Lin,Dan Li,Peiyuan Luo,Laisheng Li
we reported a new phenylcarbamoylethylenediureido-β-cyclodextrin CSP (PUCDP) for HPLC, which was conveniently prepared by immobilizing the addition product of diethylenetriamino-β-CD with 3,5-dichlorophenyl isocyanate onto silica gel. After structural characterization, the chromatographic performance of PUCDP was preliminarily evaluated by using common 11 triazole pesticides as probes. The results showed that PUCDP had strong chiral separation abilities for all triazoles ( R s=1.44–3.70). Especially, it could first completely separate triazoles with two chiral centers such as cyproconazole, triadimenol, metconazole, paclobutrazol and bitertanol, into four peaks, respectively. Some effects on the separation and detection were investigated, including the composition of mobile phase, pH value, column temperature, and absorption wavelength. Based on comprehensive optimization of chromatographic conditions and a homemade PUCDP column, we established a new method for the simultaneous separation and determination of twelve triazole pesticide enantiomeric residues (flutriafol, cyproconazole, tebuconazole, and metconazole) in nine kinds of fruits and vegetables (apple, banana, orange, pear, cucumber, green pepper, tomato, wax gourd, cabbage). After simple QuEChERS pretreatment, samples were analyzed under the optimized conditions of a gradient elution program with acetonitrile-water (ACN H 2 O) containing 0.5 % triethylammonium acetate (TEAA) buffer (pH=6.30) as the mobile phase at a flow rate of 0.5 mL min -1 , a photo-diode array (PDA) detector at 220 nm, column temperature at 20 °C and an injection volume of 20 μL. The method exhibited good linear relationship ( R 2 =0.9941–0.9999) in the range of 0.5–60 mg l -1 for all triazole enantiomers. The limits of detection (LODs) and limits of quantification (LOQs) ranged from 0.05 to 0.10 mg kg -1 and 0.15 to 0.30 mg kg -1 , respectively. The average recoveries of all enantiomers were 81.60 %-104.08 % with intra -day and inter -day relative standard deviations (RSDs) of 1.91 %-3.50 % ( n = 5) and 2.05 %-4.49 % ( n = 15), respectively. The determination of 54 actual samples indicated the practicality of the method. This simultaneous determination method could save analysis time, and reduce solvent consumption with high analysis efficiency. The excellent performance of PUCDP was mainly related to the rich hydrogen bonds (donors and acceptors), the large π-π stacking and the extended cavity derived from the diphenylurea groups, which was consistent with the results of AutoDock molecular docking.
研究领域:
分析化学
·
色谱技术
·
农药残留分析
·
食品安全
·
手性分离技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）