Microorganisms
·
2025
·Vol. 13
·期 7
·1698
·
IF 4.2
Yue Cheng,Qian Fu,Junjia Xu,Xinhua Niu,Lin Liu,Jiaqi Wang,Jingwen Quan,Qingyue Yu,Baoyan Chi,Haitao Li,Rongmei Liu
Prometryn and acetochlor are herbicides used to control weeds in farmlands and other areas. They enter the soil through direct application, residual accumulation in crops, and atmospheric deposition. The pollution of their residues in the environment has attracted people’s attention. Bioremediation is one of the main methods to solve such problems. In this study, the effects of prometryn and acetochlor-degrading strain NM-1 on soil enzymes, soil microbial communities, and physiological indexes of soybean seedlings during soil remediation were studied, and the relationship between them was discussed. The results showed that 81.54% of prometryn (50 mg·L−1) and 89.47% of acetochlor (50 mg·L−1) were degraded within 15 days after NM-1 inoculation in soil. NM-1 positively affected soil enzyme activities and soil microbial communities, and the abundance of beneficial bacteria in soil increased. More importantly, the inoculation of strain NM-1 under prometryn and acetochlor stress significantly increased plant height, root length, root volume, water content, chlorophyll concentration, and root activity of soybean. The results of these studies showed that the NM-1 strain showed significant potential in bioremediation in order to provide technical support for solving the problem of prometryn and acetochlor pollution.
研究领域:
环境微生物学
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土壤科学
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生物修复
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农业化学
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DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2023
·Vol. 17
·期 6
·5972-5979
·
IF 3.4
Zhao Shujun,Liu Wei,Song Dapeng
The detection of the herbicide propachlor in environmental and food samples is critical due to its potential toxic effects. In this study, a sensitive and selective electrochemical sensor based on a molecularly imprinted polymer (MIP), polyethyleneimine-treated multi-walled carbon nanotubes (PEI-MWCNTs), and gold nanoparticles (AuNPs) was developed for propachlor detection. The PEI-MWCNTs enhanced the conductivity of the sensor, while AuNPs promoted electron transfer during the detection process, resulting in a lower detection limit (78 nM) and a wider linear range (0.5 µM to 70 µM) compared to traditional sensors. Furthermore, the sensor exhibited high selectivity against other herbicides, with interference suppression percentages above 92% for common contaminants. The performance of the sensor was further improved by integrating an XGBoost machine learning algorithm for data analysis, which improved the predictive accuracy of propachlor concentration. Reproducibility tests on three independently prepared sensors showed a relative standard deviation (RSD) below 5%. This innovative approach presents a significant advancement in the field of propachlor detection, providing an effective tool for environmental monitoring and food safety.
研究领域:
分析化学
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环境科学
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食品科学
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机器学习
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Yonghong Zha,Yansong Li,Pan Hu,Shiying Lu,Honglin Ren,Zengshan Liu,Hualin Yang,Yu Zhou
Given the great harm of pesticide residues to the environment and public health, exploring ultrasensitive and low-cost methods for their quantitative analysis becomes intensely necessary. Herein, we proposed a double-functionalized gold nanoparticle (AuNP) probe as a signal amplification immunoassay for the detection of acetochlor (ATC), metolachlor, and propisochlor. The AuNP was modified with IgG and fluorophore-labeled duplex DNA by a polyadenine-based freezing method. The quenched fluorescence can be effectively recovered via duplex-specific nuclease (DSN) with excellent cleaving activity. This approach provided limits of detection (LODs) down to 0.03 ng/mL for ATC, 0.10 ng/mL for metolachlor, 0.14 ng/mL for propisochlor, and 0.08 ng/mL for their mixture. The average recoveries of ATC, metolachlor, and propisochlor were 93.0–106.6% from a corn sample, which are in good agreement with the commercial kit (R2 = 0.9995). This “turn-off” fluorescence immunoassay presents considerable potential in the analysis of chloroacetamide herbicide due to its simple process of probe preparing and ultrahigh sensitivity.
研究领域:
分析化学
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环境科学
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公共卫生
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纳米技术
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分子生物学
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DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2026
·Vol. 14
·期 2
·121850
·
IF 7.5
Yong-mei Zhang,Ping-ping Fan,Ling-zhi Zhang,Wei Yu,Song Xia,Fa-li Liu,Xue-de Li
Herbicide is one of the most widely used pesticides in China, yet its spatiotemporal dynamics and associated risks in Chaohu Lake remain poorly understood. Here, we investigated the occurrence, partitioning behavior, and multi-tier risks of three amide herbicides (AHs) and two organophosphorus herbicides (OPHs) in Chaohu Lake, China, using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). All target herbicides were detected in surface water and sediments, showing significantly higher concentrations in summer than in winter and a pronounced spatial gradient, with higher levels in the western than in the eastern lake regions. The majority of the compound was butachlor (BUT), which was 14.950 µg/L in water and 21.909 µg/kg in sediment. The lowest concentration was gluconate-ammonium (GIU), which was 0.050 µg/L in water and 0.570 µg/g in sediment. Sediment-water partitioning coefficients ( K d ) correlated positively with octanol-water distribution coefficient (log k ow ) and total organic carbon (TOC), indicating strong sediment adsorption. Risk assessments revealed that AHs posed high acute ecological risks to aquatic organisms, whereas OPHs showed negligible effects. Human health risk models indicated no significant non-carcinogenic risks (NCR) from acetochlor (ACE), BUT, glyphosate (GLY), or GIU at current levels. This study reported the occurrence of HAs and OPHs in Chaohu Lake for the first time, which filled the data gap of typical herbicides in the ecosystem.
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高影响因子
Aquaculture Reports
·
2026
·
IF 3.8
Ru Liang,Qianxue Shao,Yongming Ruan,Min Wang,Jiarong Cui,Jiaojiao Xia,Yanfei Zhu,Ziyao Liu,Zengpei Lin,Hongxing Xu,Zhongxian Lu,Pingyang Zhu
The integrated "rice-aquaculture" farming system is increasingly promoted in China as a sustainable agricultural model offering "dual benefits of grain and aquaculture". However, the safety of pesticides to co-cultured aquatic species, such as the red claw crayfish ( Cherax quadricarinatus ), requires thorough investigation. This study assessed the acute and chronic toxicological effects of six widely used chemical pesticides in paddy fields—abamectin, trifloxystrobin, triflumezopyrim, glufosinate-ammonium, zinc thiazole, and pretilachlor—on C. quadricarinatus under laboratory conditions. Acute toxicity tests revealed that abamectin and trifloxystrobin were highly toxic to C. quadricarinatus . Pretilachlor exhibited moderate toxicity. In contrast, triflumezopyrim, glufosinate-ammonium, and zinc thiazole demonstrated low acute toxicity. Subsequent chronic toxicity assessments (7-day exposure followed by a 14-day recovery) indicated that sub-lethal concentrations of triflumezopyrim, glufosinate-ammonium, and zinc thiazole induced neurotoxicity in C. quadricarinatus . Additionally, triflumezopyrim exposure led to oxidative stress, as evidenced by altered antioxidant enzyme activities and increased lipid peroxidation and DNA damage. Most notably, these sub-lethal effects-including significant changes in growth parameters (SGR, HSI), neurotoxicity (AChE inhibition), oxidative stress (SOD, CAT, MDA), and genotoxicity (8-OHdG) were largely reversible, consistently returning to control levels after a 14-day recovery in clean water. This demonstrated resilience provides a strong scientific basis for the periodic and safe application of these low-toxicity agents in integrated rice-crayfish system, while the highly toxic abamectin, trifloxystrobin, and moderate toxic pretilachlor pose significant risks and their use should be limited. This research provides a scientific basis for pesticide selection to support the sustainable development of integrated rice-red claw crayfish farming systems, contributing to the protection of aquatic ecosystems while ensuring the economic benefits of agricultural production.
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 5
·1-14
·
IF 5.3
Bu Ziyun,Si Qiankang,Lu Xiaolin,Wang Pei,Wu Dezhen,Mei Yingying
A novel monolithic capillary column (AuNPs@CMIP(poly-Chitosan)@capillary) with hybridized chiral molecular imprinted polymers (CMIPs) as stationary phase was prepared using S-metolachlor (S-MET) as template, chitosan as functional monomer, glutaraldehyde as cross-linker, and aminated-gold nanoparticles (NH 2 -AuNPs) as nanocarriers. The capillary electrochromatography (CEC) system constructed by AuNPs@CMIP(poly-Chitosan)@capillary achieved the efficient chiral separation of MET (resolution (Rs) = 9.83) as well as separation of its various structural analogs (Rs > 1.58). In addition, AuNPs@CMIP(poly-Chitosan)@capillary exhibits satisfactory reproducibility and stability. The relative standard deviations (RSDs) of migration times and Rs for intra-day, inter-day, and inter-column were all less than 6.1%, and no significant decline in separation performance was observed after 21 days of storage. NH 2 -AuNPs, with their large specific surface area and stable structure, can provide abundant binding sites and play a supporting role, reducing the deformation and collapse of CMIPs’ cavities, and further ensuring the structural stability and separation performance of the stationary phase. Finally, parameters such as imprinting factor and selectivity factor were calculated by adsorption experiments, and the CEC separation mechanism was discussed. The chitosan CMIPs-based capillary monolithic column developed in this work has rarely been reported and is an innovative attempt, providing a feasible and promising approach for the development of CEC chiral monolithic stationary phases. Graphical abstract
研究领域:
分析化学
·
材料科学
·
环境科学
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xinyue Huang,Lei Jiang,Lin Yuan,Wensi Ma,Xintao Cui,Jiaxuan Liu,Xiuling Ji,YujiaoTu
Pesticides are chemicals widely utilized in various industries, particularly agriculture, to manage pests and enhance crop yields. However, the excessive use of pesticides, such as phoxim, poses threats to the environment, food safety, and human health. Thus, it is crucial to use pesticides judiciously, limiting their application to what is necessary. Fluorescent probes have garnered attention for their cost-effectiveness, straightforward preparation, and high sensitivity, positioning them as promising candidates for detecting phoxim. In this context, orange ratiometric fluorescent carbon dots were synthesized using rhodamine B and NaOH as precursors through a hydrothermal method. The synthesized Rh B Na CDs were then purified via dialysis, thin-layer chromatography, and chromatographic columns to develop an accurate and precise ratiometric fluorescent probe. This nanoprobe demonstrated excellent linear ranges for detecting phoxim levels in various food samples. It exhibited a low detection limit of 4.486 nM for phoxim concentrations ranging from 0 to 25 μM and can accurately detect concentrations between 25 and 250 μM. Moreover, it proved sensitive in identifying phoxim content in samples of red grapes, green grapes, tomatoes, and pitayas.
研究领域:
分析化学
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环境科学
·
食品科学
·
纳米技术
·
农药检测
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DOI
PubMed
高影响因子
SENSORS
·
2025
·Vol. 25
·期 18
·5838
·
IF 3.5
Xiu Liu,Xing Tang,Hongjun Chen,Xiang Wu,Zitong Fu,Mingyu Peng,Chenzhong Jin,Jun Guo
Given the significance of safeners and their potential to emit harmful substances into the environment, it is essential to develop suitable analytical methods for detecting these compounds. This study presents a molecularly imprinted electrochemical sensor designed for the sensitive and rapid detection of fenclorim (FM), a type of safener. Titanium carbide nanomaterials (Ti3C2Tx) were electrochemically deposited onto the glassy carbon electrode (GCE) to enhance electron transfer. Subsequently, molecularly imprinted polymers were fabricated through the electropolymerization of catechol in the presence of FM. The electrochemical behavior of each modified electrode was investigated using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Under optimized experimental conditions, the MIP/Ti3C2Tx/GCE sensor demonstrated a linear relationship with FM concentration ranging from 5 to 300 nM, with a limit of detection (LOD) of 1.56 nM (S/N = 3). Additionally, the sensor demonstrated excellent selectivity, stability, and reproducibility for FM detection and was successfully utilized for quantifying FM in real water samples.
研究领域:
分析化学
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环境监测
·
纳米材料
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电化学传感
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食品安全
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DOI
PubMed
高影响因子
Fishes
·
2025
·Vol. 10
·期 6
·248
·
IF 2.4
Qianxue Shao,Yongming Ruan,Ru Liang,Ruixin Jin,Zhixi Jin,Lin Xie,Yongqing Chi,Jiaojiao Xia,Pingyang Zhu
Integrated rice–fish farming, crucial for sustainable agriculture, relies on the judicious use of pesticide. This study evaluates the toxicity of six common rice-field pesticides onProcypris merus(rice flower carp), a key species in these systems. We conducted acute and chronic toxicity tests, assessing survival, growth, oxidative stress (SOD, CAT, MDA, 8-OHdG), and neurotoxicity (AChE). Results revealed a spectrum of toxicity: abamectin and trifloxystrobin were highly toxic; pretilachlor was moderately so; and glufosinate-ammonium, triflumezopyrim, and thiazole zinc were low. Notably, triflumezopyrim induced significant oxidative stress and DNA damage, while all three low-toxicity pesticides inhibited AChE activity, indicating potential neurotoxicity. Despite these effects, all observed toxicities were reversible within 7–14 days. Considering that the tested concentrations exceeded typical field application rates, glufosinate-ammonium, triflumezopyrim, and thiazole zinc are deemed relatively safe forP. merusat recommended dosages. Our findings provide critical insights for optimizing pesticide selection in rice–fish farming, balancing pest control with ecological safety, thereby informing sustainable agricultural practices.
研究领域:
生态毒理学
·
水产养殖
·
农业科学
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DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2020
·
IF 3.721
Yujie Zhang,Yunlong Huang,Ling Fu,Jiaoyan Qiu,Zhuqing Wang,Aiguo Wu
Paraquat (PQ) is a kind of herbicide, which is highly toxic to human body. In this work, we proposed a fast and simple detection method for PQ in aqueous samples based on electrostatic interactions between modified gold nanoparticles (Au NPs) and PQ. Au NPs were first modified with sodium 3-mercapto-1-propanesulfonate (Au NPs-3MPS). 3MPS with negative charge can interact with nitrogen atoms of PQ with positive charge via electrostatic force. As a result, Au NPs-3MPS aggregated together. Accordingly, the color of Au NPs-3MPS colloid changed from red to blue-gray, and the UV–vis absorption spectrum changed accordingly. The detection limit of PQ is 0.1 mg/L by naked eyes, and 0.001 mg/L by UV–vis spectroscopy. Moreover, this detection method has good specific selectivity and anti-interference performance, and it has been successfully used for detecting PQ in real environmental samples and fruit juice samples.
研究领域:
环境科学
·
分析化学
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DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 8
·1280
·
IF 4.7
Yifei Sun,Yan Tang,Zetao Chen,Miaoxiu Ge,Wei Xiong,Luhong Wen
Abuse of herbicides in food safety is a vital concern that has an influence on the sustainable development of the world. This work presents, a modified ionization method with separation of the sample and carrier gas inlets, which was utilized for efficient ionization and analyte transfer of herbicides in crops. The working parameters of voltage, injective distance, desorption temperature, and the carrier gas flow rate were optimized to achieve the high efficiency of the transfer and ionization of the analyte. When it was applied in the analysis of herbicides in laboratory, the method exhibited excellent performance in achieving the quantitative detection of herbicides in solutions and residues spiked in an actual matrix with a limit of quantification of 1–20 μg/kg and relative standard deviations of less than 15%. Although a simple QuEchERS process was used, the programmable heating platform ensured efficient gasification and transfer of the target analyte, with the advantages of high speed and selectivity, avoiding the noted matrix effect. The method exhibited a relatively acceptable performance by using air as the discharged gas (open air). It could be used to monitor herbicide residues in the growth stage via on-site non-destructive analysis, which obtained low LODs by dissociating the herbicides from the crops without any pretreatment. It showed great potential for the supervision of the food safety market by achieving non-destructive detection of crops anytime and anywhere. This finding may provide new insights into the determination of pesticide emergence and rice quality assessment.
研究领域:
分析化学
·
食品安全
·
环境科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2015
·
IF 5.636
Sihua Qian,Hengwei Lin
Due to relatively low persistence and high effectiveness for insect and pest eradication, organophosphates (OPs) and carbamates are the two major classes of pesticides that broadly used in agriculture. Hence, the sensitive and selective detection of OPs and carbamates is highly significant. In this current study, a colorimetric sensor array comprising five inexpensive and commercially available thiocholine and H2O2 sensitive indicators for the simultaneous detection and identification of OPs and carbamates is developed. The sensing mechanism of this array is based on the irreversible inhibition capability of OPs and carbamates to the activity of acetylcholinesterase (AChE), preventing production of thiocholine and H2O2 from S-acetylthiocholine and acetylcholine and thus resulting in decreased or no color reactions to thiocholine and H2O2 sensitive indicators. Through recognition patterns and standard statistical methods (i.e., hierarchical clustering analysis and principal component analysis), the as-developed array demonstrates not only discrimination of OPs and carbamates from other kinds of pesticides but, more interestingly, identification of them exactly from each other. Moreover, this array is experimentally confirmed to have high selectivity and sensitivity, good anti-interference capability, and potential applications in real samples for OPs and carbamates.
研究领域:
分析化学
·
环境科学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）