Gang Tao,Lina Liu,Yejing Wang,Huaipu Chang,Ping Zhao,Hua Zuo,Huawei He
Sericin from Bombyx mori cocoon has good hydrophilicity, reaction activity, biocompatibility, and biodegradability, which has shown great potentials for biomedical materials. Here, an ultraviolet light-assisted in situ synthesis approach is developed to immobilize silver nanoparticles on the surface of sericin gel. The amount of silver nanoparticles immobilized on the surface of sericin gel could be regulated by the irradiation time. The porous structure and property of sericin gel were not affected by the modification of AgNPs, as evidenced by the observation of scanning electron microscopy, X-ray diffractometry, and Fourier transform infrared spectroscopy. Differential scanning calorimetry analysis showed that the modification of AgNPs increased the thermal stability of sericin gel. The growth curve of bacteria and inhibition zone assays suggested that the sericin gel modified with AgNPs had good antimicrobial activities against both Gram-negative and Gram-positive bacteria. This novel sericin has shown a great potential for biomedical purpose.
Tao Gang,Wang Yejing,Liu Lina,Chang Huaipu,Zhao Ping,He Huawei
Sericin has shown a great potential as a biomaterial in biomedical application due to its good hydrophilicity, reactivity and biodegradability. To solve the adherence and growth of microorganisms on sericin gel, here we prepared a polyelectrolyte film with interactive arrangement of
opposite charges poly(dimethyldiallylammonium chloride) (PDDA) and poly(acrylic acid) (PAA) coated on the surface of sericin gel via electrostatic interaction. AgNPs was then synthesized with the assistance of UV in situ on the polyelectrolyte film to endow the antibacterial
activity of sericin gel. SEM revealed the polyelectrolyte film could effectively facilitate the high density growth of AgNPs as a 3-D matrix and the modification of polyelectrolyte and AgNPs did not alter the porous characteristics of sericin gel. XRD studies suggested AgNPs synthesized on
the polyelectrolytes film coated sericin gel had good crystal structure. DSC analysis showed AgNPs could tightly bind with the polyelectrolytes film and sericin gel, and the binding may improve the melting temperature of AgNPs. Both bacterial inhibition zone and growth curve assay demonstrated
the sericin gel coated with AgNPs-polyelectrolytes film had an excellent and long-lasting antibacterial activity. This novel sericin gel has shown great potentials in biomedical application such as wound healing.
危险品化学品经营许可证（带存储）