ACS Sustainable Chemistry & Engineering
·
2019
·
IF 6.97
Jia Ke,Yingnan Zhu,Jiamin Zhang,Jing Yang,Hongshuang Guo,Weiqiang Zhao,Chiyu Wen,Lei Zhang
Silk is an attractive natural fiber due to its excellent mechanical properties and biocompatibility. Xenobiotic modification of pristine silk is gaining significant attention to meet specific demands. The silk payload of xenobiotics is affected by their uptake efficiency, biodistribution, metabolism, and excretion. Herein, we systematically evaluated the effect of the nanoparticle size (20–100 nm) on the biodistribution and silk production by introducing silver nanoparticles (AgNPs) into the silkworm diet. Interestingly, the silk payload of AgNPs is highly dependent on their overall size, where 50 nm AgNPs are prone to accumulate in silk glands and reveal the highest content in the produced silk, qualifying silk antibacterial activity against both Gram-positive and Gram-negative bacteria. These findings provide new insights to understand how nanoparticle properties influence the incorporation of nanomaterials into silk and can help optimize the material design.
研究领域:
生物材料学
·
纳米生物学
·
昆虫学
✓ 已验证
DOI
高影响因子
Gels
·
2022
·Vol. 9
·期 1
·4
·
IF 4.432
Jia Li,Bo-Xiang Wang,De-Hong Cheng,Yue Zhang,Qiang Yao,Xin-bin Ji,Jing Guo,Yan-Hua Lu
To improve the versatility of the sodium alginate-loaded bio-hydrogels, Antheraea pernyi silk gland protein/sodium alginate drug-loaded hydrogels were prepared by using an eco-friendly multiple network cross-link technology. Fourier transform infrared (FT-IR) spectroscopy and UV-Vis spectrophotometer were used separately to evaluate the chemical structure and drug release behavior of drug-loaded hydrogels. The antibacterial drug carrier gels were evaluated by using inhibition zone test against theS. aureusandE. coli. The CCK-8 assay was used to assess the biocompatibility of drug loaded hydrogels. The FT-IR results showed that there was a strong interaction within the drug loaded hydrogels, and the ASGP was beneficial to enhance the interaction within the drug loaded hydrogels. UV-Vis spectrophotometer results indicated the cumulative release reached 80% within 400 min. Antibacterial bio-hydrogels had a good antibacterial property, especially the antibacterial bio-hydrogels with bacitracin exhibits superior to other antibacterial agents. The drug-loaded bio-hydrogels exhibited the adhesion and proliferation of RSC96 cells and perfected biocompatibility. This provides a new idea for further research and development of tissue-friendly drug-loaded biomaterials.Keywords:antheraea pernyi silk gland protein;sodium alginate;multiple network hydrogel;tissue engineering;drug release systems
研究领域:
材料科学
·
生物医学工程
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Toxics
·
2025
·Vol. 13
·期 6
·427
·
IF 4.1
Xinyi Xie,Jiayin Hou,Meng Li,Zhiyu Liu,Mengai He,Chenxi Li,Xiaohua Du,Liezhong Chen
Phoxim is a pesticide extensively applied in mulberry fields, and residues may persist on leaves even after the recommended pre-harvest interval. However, the potential risks of these residues toBombyx moriL. (Lepidoptera: Bombycidae) have long been overlooked. The results demonstrated that chronic low-dose exposure from the second to fifth instars significantly impaired silkworm development and silk production. Specifically, larvae in the 0.316 μg/mL treatment group (1/2 LC50) exhibited a significant reduction in body weight, while the cocoon shell ratio was significantly decreased in both the 0.079 μg/mL (1/8 LC50) and 1/2 LC50groups. Cocoon deformities were observed in the 0.032 μg/mL (1/20 LC50), 1/8 LC50, and 1/2 LC50groups. Histopathological analysis revealed silk gland damage in the treatment groups, with severity increasing with higher phoxim concentrations. Biochemical analyses indicated elevated malondialdehyde (MDA) levels accompanied by increased superoxide dismutase (SOD) and peroxidase (POD) activities. Notably, phoxim exposure selectively reduced juvenile hormone (JH) titers without affecting ecdysone titers. JH-regulated genes including the receptorsMet1andMet2, and transcription factorsKr-h1andDimmwere downregulated, accompanied by suppressed expression of the fibroin synthesis geneFib-H. These results collectively indicate that chronic low-concentration phoxim exposure disrupts endocrine regulation, damages silk gland integrity, and ultimately reduces silk production in silkworm.
研究领域:
昆虫学
·
毒理学
·
分子生物学
·
内分泌学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Ling Jia,Wei Lu,Dan Hu,Min Feng,Aoming Wang,Ruolin Wang,Hao Sun,Pan Wang,Qingyou Xia,Sanyuan Ma
Synthetic biology is an eco-friendly and sustainable approach for the production of compounds, particularly used when the production processes involve toxic reagents. In this study, we used the silk gland of silkworm to produce indigoidine, a valuable natural blue pigment that cannot be synthesized naturally in animals. We genetically engineered these silkworms by integrating the indigoidine synthetase ( idgS ) gene from S. lavendulae and the PPTase ( Sfp ) gene from B. subtilis into the silkworm genome. In the resulting Blue silkworm, indigoidine was detected at a high level in the posterior silk gland (PSG), spanning all developmental stages from larvae to adults, without affecting silkworm growth or development. This synthesized indigoidine was secreted from the silk gland and subsequently stored in the fat body, with only a small fraction being excreted by the Malpighian tubule. Metabolomic analysis revealed that Blue silkworm efficiently synthesized indigoidine by upregulating l -glutamine, the precursor of indigoidine, and succinate, which is related to energy metabolism in the PSG. This study represents the first synthesis of indigoidine in an animal and therefore opens a new avenue for the biosynthesis of natural blue pigments and other valuable small molecules.
研究领域:
合成生物学
·
遗传学
·
生物技术
·
昆虫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROBIAL ECOLOGY
·
2024
·Vol. 87
·期 1
·1-15
·
IF 3.3
Zhao Likun,Zhang Shanfeng,Xiao Ruoyi,Zhang Chao,Lyu Zhitang,Zhang Feng
Spiders host a diverse range of bacteria in their guts and other tissues, which have been found to play a significant role in their fitness. This study aimed to investigate the community diversity and functional characteristics of spider-associated bacteria in four tissues of Heteropoda venatoria using HTS of the 16S rRNA gene and culturomics technologies, as well as the functional verification of the isolated strains. The results of HTS showed that the spider-associated bacteria in different tissues belonged to 34 phyla, 72 classes, 170 orders, 277 families, and 458 genera. Bacillus was found to be the most abundant bacteria in the venom gland, silk gland, and ovary, while Stenotrophomonas , Acinetobacter , and Sphingomonas were dominant in the gut microbiota . Based on the amplicon sequencing results, 21 distinct cultivation conditions were developed using culturomics to isolate bacteria from the ovary, gut, venom gland, and silk gland. A total of 119 bacterial strains, representing 4 phyla and 25 genera, with Bacillus and Serratia as the dominant genera, were isolated. Five strains exhibited high efficiency in degrading pesticides in the in vitro experiments. Out of the 119 isolates, 28 exhibited antibacterial activity against at least one of the tested bacterial strains, including the pathogenic bacteria Staphylococcus aureus , Acinetobacter baumanii , and Enterococcus faecalis . The study also identified three strains, GL312, PL211, and PL316, which exhibited significant cytotoxicity against MGC-803. The crude extract from the fermentation broth of strain PL316 was found to effectively induce apoptosis in MGC-803 cells. Overall, this study offers a comprehensive understanding of the bacterial community structure associated with H. venatoria . It also provides valuable insights into discovering novel antitumor natural products for gastric cancer and xenobiotic-degrading bacteria of spiders.
研究领域:
微生物学
·
分子生物学
·
生态学
·
生物技术
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Gels
·
2022
·Vol. 8
·期 7
·398
·
IF 4.432
Jia Li,Bo-Xiang Wang,De-Hong Cheng,Yan-Hua Lu
The Antheraea Pernyi silk gland protein originates from natural organisms and synthesized by tussah silk glands and has widely potential biomaterial applications due to the superior biocompatibility. This study investigates the Antheraea Pernyi silk gland protein-based drug-loaded bio-hydrogels for bioengineered tissue fabricated by using an eco-friendly method without the harsh extracting process and the usage of toxic chemicals. The drug-loaded bio-hydrogels exhibited a porous structure and interconnected pore walls. The swelling ratio and water absorption of drug-loaded bio-hydrogels were, respectively, above 95% and 1.5 × 103%. The cumulative release of drug loaded hydrogels all reached more than 90% within 4 h, and this indicates the potential of drug-loaded hydrogels as future drug-carrying biomaterials. RSC96 Schwann cells cultured on drug-loaded hydrogels for 72 h under cell culture medium show no toxic effects and more pro-proliferative effects. The results suggest the suitability of drug-loaded bio-hydrogels as natural biopolymer for the potential in vitro RSC96 cell culture platform and other biomaterial applications.
研究领域:
生物材料
·
组织工程
·
药物递送系统
·
细胞生物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）