RSC Advances
·
2021
·Vol. 11
·期 14
·8250-8254
·
IF 3.361
Huiying Chen,Jianzhi Zhao,Junzhi Lin,Baoli Dong,Hui Li,Bing Geng,Mei Yan
Lipid droplets (LDs) are storage organelles at the centre of lipid and energy homeostasis, which act as vital hubs of cellular metabolism and the key to maintaining lipid and energy homeostasis. We synthesized a new two-photon fluorescent probe (CIV) that could detect the viscosity of lipid droplets. The probe is constructed via the typical ICT system of D–π–A using carbazole as the donor and imidazole as the acceptor. With the increase in viscosity from PBS to 99% glycerol, the fluorescence intensity of CIV increased by 13-fold, showing sensitivity and specificity towards viscosity. In addition, CIV showed low toxicity and excellent biocompatibility in cytotoxicity tests, and was successfully used for living cell LD imaging. Taken together, the results widen the way for the development of novel fluorescent probe-based the visualization LDs and detection in solutions, physiology and pathology.
研究领域:
生物化学
·
细胞生物学
·
医学影像学
✓ 已验证
DOI
PubMed
高影响因子
Applied Materials Today
·
2021
·
IF 10.041
Minghao Ni,Ming Gong,Xiang Li,Jinlou Gu,Bo Li,Ye Chen
Metal-organic frameworks (MOFs) have attracted significant attention thanks to their excellent physical and chemical properties. Metal-organic frames are widely used in optics. For example, metal-organic frameworks can be used in fluorescent probes to detect and treat diseases, and can also be used to make solid-state light-emitting devices. Considering the high stability and easy synthesis of Zr-based UiO-66 MOF as well as the strong fluorescent emission of porphyrin molecules, the TCPP-incorporated UiO frameworks would inherit these advantages and exhibit intriguing optical performance. Therefore, in this paper, TCPP was doped into UiO-66, and the optical properties of the resulting MOF structure were observed. To obtain the optimal doping condition, three doping concentrations of TCPP were used. Besides the structural and morphological characterization by X-ray diffraction and scanning electron microscopy, more attentions have paid on the optical testing based on absorption and fluorescence spectroscopy, two-photon fluorescence spectroscopy and time-resolved spectroscopy. As a result, the TCPP-doped MOFs exhibited good fluorescence properties and satisfied our previous expectations of nonlinear optical properties and two-photon absorption. This work would pave a new way in constructing various MOFs-based solid-state devices with widespread optical applications.
研究领域:
材料科学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF LUMINESCENCE
·
2020
·
IF 3.28
Dan Yue,Xin Zhang,Yiqun Tan,Zhenling Wang,Yilei Zhang
A novel two-photon fluorescent probe (ZC-F2) for selective recognizing Zn 2+ and Cd 2+ was designed and synthesized. By tuning the intramolecular charge transfer (ICT), ZC-F2 can recognize Zn 2+ and Cd 2+ through different emission colors in acetonitrile , the detection limit of Zn 2+ and Cd 2+ are 11.25 nM and 1.80 nM respectively. Moreover, ZC-F2 exhibits strong two-photon fluorescence emission when being excited by a femtosecond laser in the range of 700–860 nm, and its two-photon absorption (TPA) cross-section is studied. The cell-imaging experiments show that ZC-F2 has high sensitivity to Zn 2+ and Cd 2+ , indicating its potential applications in environmental and biological detection.
研究领域:
化学
·
生物检测
·
环境科学
✓ 已验证
DOI
高影响因子
JOURNAL OF COORDINATION CHEMISTRY
·
2020
·
IF 1.41
Ting-Ting Zang,Jia-Peng Cao,Ze-Yu Du,Hua Mei,Yan Xu
Two new Schiff-base modified vanadium complexes, [VIVO(C7 H5O)2(N2C6H10)] (1) and [VVO2(C7H5O)(N2C6H12)] (2), were synthesized under hydrothermal conditions, in which salicylaldehyde and cyclohexanediamine were used directly to form Schiff-base ligands during the reactions. The structures of 1 and 2 were characterized by EA, FT-IR, UV-vis, SCXRD and PXRD, EPR spectroscopy and magnetic susceptibility measurement. Due to the good optical activity of Schiff-base ligands and the existence of conjugated benzene rings in 1 and 2, the delocalized electronic effects between Schiff-base ligands and vanadium can be improved. Therefore, the third-order non-linear optical (NLO) properties of these complexes were investigated. The results of the open aperture Z-scan tests show that the two-photon absorption (TPA) cross-sections (σ) of the complexes are 1033 and 1041GM, respectively, which indicated that they both have potential applications as NLO materials.
研究领域:
化学
·
材料科学
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 17
·14325-14331
·
IF 3.201
Ruiqing Feng,Longlong Li,Bing Li,Jinhui Li,Dan Peng,Yitao Yu,Qiuhong Mu,Ning Zhao,Xiaoqiang Yu,Zhenhua Wang
We have synthesized a two-photon organic small-molecule probe (DMI), which has a considerable two-photon absorption cross section (δ) value and selectively stained endogenous RNA in the nucleolus and the cytoplasm of living cells with outstanding membrane permeability. The titrations, fluorescence imaging and RNase digest test indicate markedly higher affinity for endogenous RNA. The double assay of DMI and SYTOX Blue nucleic acid stain (S-11348) showed that the probe possesses excellent membrane permeability to living cells, and a co-staining experiment with the classic DNA-staining dye DAPI indicated that it has excellent counterstain compatibility. Moreover, DMI exhibited good photostability when continuously illuminated by constant femtosecond pulses. These results demonstrate that DMI is an attractive two-photon fluorescent probe for visualizing RNA in living cells.
研究领域:
生物化学
·
细胞生物学
·
分子影像学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2022
·Vol. 147
·期 11
·2558-2566
·
IF 5.227
Tongtong Zhu,Lei Cao,Zhenqiao Zhou,Hanzhou Guo,Mingfeng Ge,Wen-Fei Dong,Li Li
Cellular imaging using carbon dots is an important research method in several fields. Herein, green-emissive carbon quantum dots (G-CDs) with a pretty high absolute quantum yield (QY) were fabricated via a one-step solvothermal method by using m-phenylenediamine and concentrated hydrochloric acid. G-CDs displayed strong green fluorescence with excitation/emission peaks at 460/500 nm, and their absolute quantum yield was as high as 58.65%. Further experiments suggested that the G-CDs we prepared have good solubility, excellent biocompatibility, and the capacity of rapidly imaging HeLa and 4T1 cells. Over expectations, the G-CDs could penetrate cells in only 10 s and the confocal images showed that the G-CDs could target the nucleus of cells. Moreover, by using 920 nm as the excitation wavelength, two-photon imaging has been successfully applied to 4T1 cells, overcoming the inherent limitations of single-photon imaging. The extremely high absolute quantum efficiency, ultra-fast imaging speed, and two-photon imaging capability make the G-CDs have good application potential in biomedical analysis and the clinical diagnostic field.
研究领域:
生物医学
·
材料科学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Shan He,Wen-Le Fang,Xiao-Feng Guo,Hong Wang
Redox homeostasis is the guarantee for the normal operation of living organisms. Given its importance, monitoring dynamic changes of redox state will facilitate the study of relevant physiological and pathological processes. Herein, a two-photon fluorescent probe NA-Se with selenomorpholine as the response site has been developed for reversible detection of redox state. NA-Se exhibits outstanding analytical performance, including a rapid response to ClO − within 8 s, a detection limit as low as 13.3 nM, and at least 10 redox reversible cycles. Furthermore, NA-Se has improved water solubility and extremely low cytotoxicity, making it ideal for bioimaging. Finally, NA-Se has been successfully applied to monitor the ClO − /GSH redox cycle in living cells and zebrafish. This work may provide an effective tool for studying redox state and related diseases in vivo.
研究领域:
生物化学
·
医学影像
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 81
·77849-77853
·
IF 3.289
Dan Liu,Na Zhang,Xinyu Zhang,Shuai Kong,Jianhua Yu,Zhichao Wu,Jieying Wu,Yupeng Tian,Hongping Zhou
A series of new zinc(II) complexes (Dyes 1–3) were obtained by self-assembly of the donor-bridge-donor (D–π–D) type ligand L with ZnX2 (X = Br, I, SCN). The single crystal of Dye 3 was obtained by slow evaporation of a mixture solution of methanol and dichloromethane. The molecular structure has been determined by X-ray crystallography. Hydrogen bonds (C–H⋯N, C–H⋯S) play significant roles in the final topological structures. Linear and nonlinear optical properties of the ligand and complexes were investigated in details. Experimental results revealed that effective two-photon absorption (TPA) cross sections of these complexes are larger than that of ligand L (18.7 GM) with the maximum values of 88.7 GM (Dye 1), 41.9 GM (Dye 2) and 46.5 GM (Dye 3) in DMF, respectively. The biological imaging result shows that L and Dyes 1–3 had excellent penetrability in living cells.
研究领域:
化学
·
材料科学
·
生物学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2012
·
IF 2.098
Lin Ma,Yunhui Sun,Duxia Cao,Huihui Chen,Zhiqiang Liu,Qi Fang
Three aurone derivatives, Z -2-[(4- N , N -dimethylaminophenyl)methylene] benzofuran-3-one ( 1 ), Z -2-[( N -ethylcarbazol-3-yl)methylene]benzofuran-3-one ( 2 ) and Z -2-[(pyren-1-yl)methylene]benzofuran-3-one ( 3 ) have been synthesized by the cyclization of 2′-hydroxychalcones. Their crystal structure, single- and two-photon related absorption and fluorescence properties have been examined. Pumped by 860 nm laser pulses in femtosecond regime, the compounds exhibit strong yellow–green two-photon excited fluorescence at 539 nm ( 1 ), 505 nm ( 2 ) and 524 nm ( 3 ) in THF with two-photon absorption (TPA) cross-section being 1536GM ( 1 ), 608GM ( 2 ) and 236GM ( 3 ).
研究领域:
有机化学
·
材料科学
·
光学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Dejia Chen,Yixuan Xu,Yating Wang,Xin Li,Dalong Yin,Lifeng Yan
Stable organic radicals, with unique electronic transitions from the ground state (D0) to the doublet excited state (D1), show promise as high-fluorescence quantum yield dyes. While organic small-molecule photosensitizers (PSs) have advanced for tumor photodynamic therapy (PDT), opportunities exist to enhance their performance and functionality. Herein, we synthesized Thiele’s fluorocarbon derivative diradicaloid TFC-I with nearly 100% PLQY and integrated it into amphiphilic polypeptide nanoparticles, P-TI, using a precursor-doping approach. P-TI demonstrated notable features including high photostability, aggregation-induced emission, bright near-infrared fluorescence, substantial quantum yield (37% PLQY), robust near-infrared two-photon absorption (∼400 GM cross section), and superior ROS generation compared to commercial PSs. In vitro and in vivo experiments confirmed that P-TI performed well in mitochondria-targeted PDT, two-photon fluorescence imaging, and biosafety. This work highlights the use of organic stable radicals with precursor-doping for efficient PDT and deep tumor tissue imaging.
研究领域:
生物医学工程
·
材料科学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Yaqi Dong,Haibin Xiao,Lingbao Xing,Chuanchen Wu,Jin Zhou,Ziyan Zhou,Yuying Liu,Shuping Zhuo,Ping Li
We herein develop a novel two-photon fluorescent probe termed L-pH for visualization of lysosomal pH within live cells. L-pH is composed of three moieties, including naphthalimide fluorophore as a fluorescence off-on response moiety, piperazine and morpholine groups as lysosomal targeting and pH responsive sites, as well as a reactive benzyl chloride segment for further lysosomal anchoring. The experimental results demonstrate that L-pH can instantaneously respond to various pH values with high sensitivity and selectivity, and has low cytotoxicity and excellent photostability. The one-photon and two-photon fluorescence imaging data indicate L-pH can preferably accumulate into lysosome and monitor the rise of lysosomal pH changes during myriad cell stress conditions, including heat shock, cell apoptosis and mitophagy. Moreover, L-pH was applied for imaging of pH difference in abdominal tissues of mice. L-pH will be a potential tool for monitoring lysosomal pH variation during lysosome-associated physiological and pathological states.
研究领域:
生物化学
·
细胞生物学
·
医学影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Today Communications
·
2020
·
IF 2.678
Shanggeng Li,Chunlin Lu,Xiangyu Wan,Shuai Zhang,Jing Li,Zhibing He,Lin Zhang
A series of new initiators with a conjugated A–π–D–π–A structure of phenothiazine and carbazole derivatives were designed and synthesized to satisfy the demand for novel high-efficiency two-photon polymerization initiators. Moreover, the optical properties and two-photon polymerization properties of such new initiators were investigated. It was found that the two-photon absorption cross section of the initiators with the best performance can reach 1315 GM at 780 nm, and the scanning speed could reach 14,000 μm s −1 at a laser energy of 30 mW. When the wavelength was 810 nm, the absorption cross-section could reach up to 1667 GM. By adjusting the laser power and scanning speed, two-photon polymerization can be achieved within a large range of parameters, and complete and clear three-dimensional structures can be obtained.
研究领域:
材料科学
·
光学工程
·
化学合成
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）