Catalysts
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2022
·Vol. 12
·期 9
·932
·
IF 4.501
Guoyun Sun,Ziyang Huang,Zhiqian Zhang,Yanfeng Liu,Jianghua Li,Guocheng Du,Xueqin Lv,Long Liu
Free enzymes often face economic problems due to their non-recyclability, which limits their applications for industrial manufacturing. Organic biopolymers are frequently used to fabricate hydrogel for enzyme immobilization due to their advantages of non-toxicity, biocompatibility, biodegradability, and flexibility. However, for highly thermostable enzymes, simple cross-linking causes either low immobilizing efficiency or low thermal stability. Herein, we developed a novel enzyme immobilization strategy with two-step cross-linked gelatin hydrogel for thermostable enzymes working at high temperature. The hydrogel was firstly “soft cross-linked” to immobilize most enzyme molecules and then “hard cross-linked” to gain strong thermal stability. We selected the enzyme diacetylchitobiose deacetylase (Dac), which was firstly derived from hyperthermophilic bacteria, to demonstrate the advantages of our method. With the optimized fabrication steps, our hydrogel showed ~87% Dac immobilization efficiency and excellent stability against heating, dehydrating, long-time storing, and massive recycling. Importantly, our hydrogel showed ~85.0% relative enzyme activity at 80 °C and retained ~65.8% activity after 10 rounds of catalysis. This strategy showed high immobilizing efficiency and strong thermal stability and we believe it could improve the industrial potential for those enzymes.Keywords:enzyme immobilization;gelatin hydrogel;two-step cross-linking;diacetylchitobiose deacetylase
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POWER SOURCES
·
2024
·
IF 8.1
Bowen Zheng,Chengwei Deng,Ruoyin Luo,Shaojie Gao,Feng Ji,Dong Wang
To simultaneously increase the mechanical strength and proton conductivity of Phosphoric acid-doped polybenzimidazole (PA-PBI), a facile two-step crosslinking approach is proposed to prepare the imidazole-rich crosslinked polybenzimidazole membranes for high-temperature proton exchange membrane fuel cells (HT-PEMFCs) in this work. Unlike the classic crosslinking approaches that usually enhance mechanical strength of membrane but lead to a decrease of proton conductivity, the mechanical strength of two-step crosslinked membrane improves from 3.93 MPa to 9.62 MPa and proton conductivity increases from 91 mS cm −1 to 183 mS cm −1 at 200 °C compared with pristine OPBI membrane. The promotion should be ascribed to the introduction of PA-affinity sites on crosslinkers and the formation of homogeneous crosslinking networks. Meanwhile, due to the crosslinked networks, the oxidative and dimensional stability of crosslinked membranes are also promoted. In addition, the two-step cross-linking method can avoid the self-reaction between cross-linking agents, thereby obtaining a more uniform cross-linking network, which is of great significance for the use of fuel cells. When the membranes are used in a fuel cells, they exhibited a maximum power density of 448.8 mW cm −2 at 160 °C (H 2 /Air, without humidification.).
研究领域:
材料科学
·
能源工程
✓ 已验证
产品关联
DOI
高影响因子
Bioresources and Bioprocessing
·
2024
·Vol. 11
·期 1
·1-14
·
IF 4.3
Chen Ming,She Weina,Zhao Xin,Chen Cheng,Zhu Benwei,Sun Yun,Yao Zhong
The Thermomyces lanuginosus lipase (TLLs) was successfully immobilized within a novel hydrogel matrix through a two-step crosslinking method. TLLs were initially crosslinked through the Schiff base reaction by oxidized carboxymethyl cellulose (OCMC). The water-soluble OCMC@TLLs complex was subsequently crosslinked by carboxymethyl chitosan (CMCSH) in a microfluidic apparatus to form the CMCHS/OCMC@TLLs microspheres. The CD (Circular Dichroism, CD) and FT-IR (Fourier Transform infrared spectroscopy, FT-IR) spectra demonstrated that the crosslinking of TLLs with OCMC resulted in a less significant impact on their structure compared to that with glutaraldehyde. CMCHS/OCMC@TLLs showed decreased catalytic performance due to the mass transfer resistance, while its thermal stability was greatly improved. The CMCHS/OCMC@TLLs were used to catalyze the lauroylation of arbutin in tetrahydrofuran. After 12 h of reaction under optimal conditions, the yield of 6′-O-lauryl arbutin reached an impressive 92.12%. The prepared 6′-O-lauryl arbutin has high lipophilicity and exhibits similar tyrosinase inhibitory activity and higher antioxidant activity compared to its parent compound.
研究领域:
生物化学
·
酶工程
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xiaocui Zhang,Dingsheng Wu,Jingli Zhang,Pan Xue,Bingyan Hou,Lingyun Ren,Danyu Liu,Pengfei Lv,Qufu Wei
Light-responsive liquid crystal elastomer (LCE) fibers hold considerable promise for use in the development of soft robotics and smart devices due to their flexibility and controllability. However, most of the inorganic materials with photothermal effects are incompatible with the liquid crystal elastomer matrix, which can result in unstable interfacial bonding between the inorganic materials and the organic liquid crystal system. Meanwhile, the multidimensional space deformation of liquid crystal elastomer fibers remains challenging. Here, numerous polymerizable graphene oxide (GO) monomers are employed as a photothermite, and the GO@LCE fiber actuators with stable organic-inorganic interfacial bonding are constructed by dry spinning and two-step crosslinking method. The actuators demonstrate remarkable photothermal conversion capability, and they also exhibit exceptional breaking strength, reusability and work capacity (400.7 kJ m −3 ), benefiting from the covalent bonding between the interfaces. As a proof-of-concept, light-controlled artificial muscle and micro crawler based on the one-dimensional linear motion of GO@LCE fiber actuators are successfully constructed. Importantly, GO@LCE knitted fabrics, which are capable of three-dimensional bending deformations in different degrees and forms, are skillfully woven through the design of knitted structures. This work can enhance new possibilities for the advancement of soft robotics and smart textiles.
研究领域:
材料科学
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纳米技术
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软体机器人
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智能材料
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纺织工程
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光热转换
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）