Food Science and Technology
·
2022
·
IF 2.602
WU Jiao,ZHAO Shan,CHEN Xin,JIU Yuanda,LIU Junfeng,GAO Jinglin,WANG Shijie
A summarized physicochemical profile of nine multifloral honeys produced by Apis cerana cerana Fabricius in Qiongzhong region located in Hainan province was exhibited, regarding pH (3.72-4.02), moisture (19.20-22.53%), fructose (34.40-38.70 g 100 g-1), glucose (31.60-35.05 g 100 g-1), sucrose (less than 3%), color (31.00-80.00 mm Pfund), ash content (0.17-0.45 g 100 g-1), soluble solid (75.87-79.10 ◦Brix), and electrical conductivity (343.67-678.33 μS cm-1). Potassium showed the highest concentration, followed by calcium. Manganese, boron, iron, aluminium, and zinc were the main micro-elements while cadmium and hydrargyrum were not detected in all honey samples. The antiradical activity was shown to be significantly negative correlated with total phenolic content (TPC) (r2 = −0.756) and total flavonoid content (TFC) (r2 = −0.477). Among all tested honey samples, sample A exhibited the highest levels of TPC (45.46 mg GAE 100 g-1), TFC (10.02 mg RE 100 g-1), and antiradical activity (DPPH IC50 = 2.63 mg mL-1). Our results will be useful in determining to set the standard for A. cerana cerana honey.
研究领域:
食品科学
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化学
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生物学
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DOI
高影响因子
Insects
·
2025
·Vol. 16
·期 9
·961
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IF 2.9
Huiman Zhang,Lele Sun,Peng Wang,Jiaoxin Xie,Yuan Guo
Simple SummaryAs a native species in China; the survival and reproduction ofApis cerana ceranaare heavily dependent on chemical signals. In the process of chemical communication, the antennae of bees serve as the primary sensing organs, and the legs also play an important role in the reception and transmission of chemical signals. In our study, scanning electron microscopy (SEM) was used to observe its legs, revealing two types of sensilla, and the ultrastructure of the tibial spur and the antennal brush was observed. The electrophysiological responses of the legs (ELG), along with Y-tube behavioral assays, were employed to validate the involvement of the bee’s legs in olfactory perception. The legs of bees at different ages showed varying degrees of significant response to nonanal and ocimene. Notably, 10-day-old bees exhibited a clear preference for nonanal and ocimene. These findings contribute to a deeper understanding of the mechanistic role of the honeybee leg in chemical signal detection, elucidating their functional characteristics in chemical communication, and providing experimental evidence for the study of bee behavioral responses and environmental adaptation.Apis cerana ceranais a key social insect, and its ability to recognize chemical signals is crucial for maintaining colony homeostasis and coordinating collective behaviors, such as foraging, nursing, and defense. The legs of insects play a significant role in gustatory perception and proximity olfactory perception. In this study, the leg sensilla ofA. c. ceranawere observed by scanning electron microscopy (SEM). Two types of sensilla were observed, including sensilla trichodea (Str I, Str II, Str III, Str IV, Str V, and Str VI) and sensilla chaetica (Sch I, Sch II, and Sch III). The two unique structures of the tibial spur (Tsp I, Tsp II) and antennal brush (Abr) are carefully observed. The electrophysiological responses of workers at different ages to diverse chemical compounds were measured via electrolegogram (ELG) recordings on their legs. The results showed that 1-day-oldA. c. ceranawas more sensitive to nonanal; 10-day-old and 25-day-oldA. c. ceranawere more sensitive to ocimene. The results of behavioral responses showed that nonanal and ocimene can significantly attract 10-day-old workers ofA. c. cerana. This study establishes a foundation for further exploration of the mechanisms by which the legs ofA. c. ceranafacilitate colony-level communication through chemical signals. It also provides an important theoretical basis for understanding their social organization and information transmission.
研究领域:
昆虫学
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神经行为学
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化学生态学
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感觉生物学
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DOI
PubMed
高影响因子
Insect Science
·
2024
·
IF 2.9
Huiting Zhao,Longlong Chen,Miaomiao Liu,Shuguo Zhao,Weihua Ma,Yusuo Jiang
Insulin receptors (InR) are an integral component of the insulin/insulin-like growth factor signaling pathway, which plays a vital role in insect development, lifespan, reproduction, and olfactory sensitivity. However, whether InR participate in the peripheral olfactory system of insects remains unclear. Recently, we found that 2-heptanone (2-HT) affects AcerInR expression, the gene for an InR protein, in Apis cerana cerana . We then examined the spatiotemporal expression profile of the gene in A. cerana cerana . The mRNA of AcerInR was primarily expressed in the antennae, wings, and legs of forager bees, which are probable chemosensory tissues. The results of fluorescence competitive binding assays, combined with site-directed mutagenesis, demonstrated that AcerOBP6 and AcerOBP14 exhibit strong binding affinities to 2-HT. Furthermore, after foragers were fed with double-stranded AcerInR , the expression levels of AcerOBP6 and AcerOBP14 decreased significantly, as did the electroantennogram responsiveness to 2-HT and some other odorants. In conclusion, our findings provide a foundation for understanding the involvement of AcerInR in the odor perception of A. cerana cerana . Moreover, they offer novel insights into the olfactory recognition mechanism in insects.
研究领域:
昆虫学
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分子生物学
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神经生物学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Yunhao Sun,Xiaojing Niu,Yuanyuan Huang,Lijun Wang,Zhenguo Liu,Xingqi Guo,Baohua Xu,Chen Wang
Tyrosine aminotransferase (TATN) is the first enzyme involved in the metabolic degradation of tyrosine , and it plays an important role in tyrosine detoxification and helps the body resist oxidative damage. However, the function of TATN in Apis cerana cerana ( A. c. cerana ) remains unclear. To explore the role of TATN in the response to pesticide and heavy metal stress in A. c. cerana , AccTATN was isolated and identified. AccTATN was highly expressed in the integument and the adult stage. Exposure to multiple pesticides and heavy metal stress upregulated AccTATN expression. RNA interference experiments showed that silencing AccTATN reduced the resistance of A. c. cerana to glyphosate and avermectins stress. The expression of antioxidant-related genes and the activity of antioxidant enzymes were reduced after AccTATN was silenced, leading to the accumulation of oxidative damage. Overexpression of the recombinant AccTATN protein in a prokaryotic system also confirmed its role in heavy metal stress and improved antioxidant capacity. Our study showed that AccTATN may promote resistance to pesticide and heavy metal stress by regulating the antioxidant capacity of A. c. cerana . This study provides a valuable theoretical basis for A. c. cerana conservation.
研究领域:
昆虫学
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毒理学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF INSECT PHYSIOLOGY
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2022
·
IF 2.608
Junjun Zhang,Zhengwei Wang,Katrina Klett,Yufeng Qu,Ken Tan
The thunder god vine , Tripterygium hypoglaucum, is a toxic nectar plant distributed across China. A terpenoid, called triptolide (TRP), found in nectar can impair honeybees’ foraging responses, dance communication, and olfactory learning. In the present study, we tested the tolerances of the native honeybee Apis cerana and the introduced honeybee A. mellifera to short-term and long-term exposure to TRP. The results showed that introduced A. mellifera is more vulnerable in fatality to high concentrations of TRP sucrose solution (5 and 10 µg TRP mL −1 ) than A. cerana . We also compared the short-term and long-term exposure effects of TRP on olfactory learning and memory between the two honeybee species, and the olfactory learning and memory of both honey bee species showed impaired performance after both 2 h or 7 days of being fed with TRP sucrose solution. However, A. cerana showed a higher tolerance and resistance to TRP toxin than A. mellifera. Our results support a coevolution hypothesis in that the native species A. cerana has higher toxin tolerance than the introduced species A. mellifera .
研究领域:
昆虫学
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毒理学
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生态学
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DOI
PubMed
高影响因子
JOURNAL OF EXPERIMENTAL BIOLOGY
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2025
·Vol. 228
·期 18
·
IF 2.6
Chen Longlong,Gong Yu,Ma Weihua,Jiang Yusuo,Zhao Huiting
The olfactory senses of Apis cerana cerana are crucial to their essential activities. This study aimed to investigate the effect of phased starvation, insulin injections and insulin silencing on the olfactory abilities of A. cerana cerana . A starvation regression curve was established; it was found that the mortality of bees increased significantly after 9 h of starvation. Measurement of insulin levels in the bees revealed a gradual decrease after 3 and 6 h of starvation, followed by a sudden rise at 9 h. Additionally, starvation induced changes in the olfactory responses of bees: starvation decreased the electroantennogram values of antennae for most odorants, changed behavioral preferences for some floral odorants and affected the expression of olfactory genes. High insulin levels enhanced electroantennogram response values of the antennae, changing most odorant selections from attraction or neutrality to avoidance. In contrast, low insulin levels decreased the electroantennogram response values of the antennae and predominantly shifted odor preferences toward attraction. These two insulin conditions resulted in opposite results, and changes in olfactory gene expression also exhibited differing trends. It was found that low-insulin conditions caused by starvation for 3 h and 6 h as well as insulin silencing exhibited similarities in multiple olfactory metrics. This suggests that starvation-induced olfactory changes may be attributed to the reduction of endogenous insulin levels. The study results reveal a strong correlation between insulin and olfaction in A. cerana cerana , providing a foundation for future investigations into the underlying regulating mechanisms of insulin/insulin-like signaling on olfaction. These findings also offer research directions for enhancing bee pollination capacity and regulating other olfactory behaviors.
研究领域:
昆虫生理学
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神经生物学
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分子内分泌学
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化感生物学
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行为生态学
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DOI
PubMed
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-9
·
IF 5.228
Wang Zhengwei,Wen Ping,Qu Yufeng,Dong Shihao,Li Jianjun,Tan Ken,Nieh James C.
Pollinators such as bees provide a critical ecosystem service that can be impaired by information about predation. We provide the first evidence for olfactory eavesdropping and avoidance of heterospecific alarm signals, alarm pheromones, at food sources in bees. We predicted that foragers could eavesdrop upon heterospecific alarm pheromones, and would detect and avoid conspicuous individual pheromone compounds, defined by abundance and their ability to persist. We show that Apis cerana foragers avoid the distinctive alarm pheromones of A. dorsata and A. mellifera , species that share the same floral resources and predators. We next examined responses to individual alarm pheromone compounds. Apis cerana foragers avoided isopentyl acetate (IPA), which is found in all three species and is the most abundant and volatile of the tested compounds. Interestingly, A. cerana also avoided an odor component, gamma-octanoic lactone (GOL), which is >150-fold less volatile than IPA. Chemical analyses confirmed that GOL is only present in A. dorsata , not in A. cerana. Electroantennogram (EAG) recordings revealed that A. cerana antennae are 10-fold more sensitive to GOL than to other tested compounds. Thus, the eavesdropping strategy is shaped by signal conspicuousness (abundance and commonality) and signal persistence (volatility).
研究领域:
生态学
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昆虫学
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化学生态学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Sheng-nan Li,Hao Wu,Rui-quan Hou,Jing-tong Zhou,Su-kun Lin,Kun-yu Zhao,Rui-fei Wang,Su-qing Huang,Zhi-xiang Zhang
O -Carboxymethyl chitosan nanoparticles ( O -CMC-NPs), which are organic pesticide carriers, have excellent application potential. Exploring the effects of O -CMC-NPs on non-target organisms, such as Apis cerana cerana , is critical for their effective application; however, such studies are limited. This study investigated the stress response of A. cerana Fabricius after O -CMC-NPs ingestion. The administration of high O -CMC-NP concentrations enhanced the activities of antioxidant and detoxifying enzymes in A. cerana , with the activity of glutathione- S -transferase increasing by 54.43 %–64.33 % after one day. The transit of O -CMC-NPs into the A. cerana midgut resulted in their deposition and adherence to the intestinal wall, as they cluster and precipitate in acidic conditions. The population of Gillianella bacteria in the middle intestine was remarkably reduced after 6 d of administration of high O -CMC-NP concentrations. Contrastingly, the abundance of Bifidobacteria and Lactobacillus in the rectum significantly increased. These results indicate that the intake of high concentrations of O -CMC-NPs causes a stress response in A. cerana and affects the relative abundance of crucial intestinal flora, which may pose a potential risk to the colony. This implies that even nanomaterials with favorable biocompatibility should be applied reasonably within a specific range to avoid adverse effects on the environment and non-target organisms in the context of large-scale research and promotion of nanomaterials.
研究领域:
纳米技术
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昆虫学
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环境科学
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产品关联
DOI
PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2025
·
IF 3.2
Liu Nan-nan,Ren Qing-dan,Li Long,Wang Li-na,Shi Guang-liang,Qin Shou-tao
Apis cerana honey from Changbai Mountain (CBS honey), produced in northeastern China, is a high-quality specialty honey that has long been recognized as a natural health product with medicinal properties. In this study, UPLC-MS/MS metabolomics and ultra-fast proteomics technologies were employed to analyze the differences in metabolites and proteins among Apis cerana honey samples collected from various Chinese regions, including Jilin, Sichuan, Hainan, Hubei, and Tibet. Through an integrated multi-omics analysis, we identified candidate characteristic compounds, including 12 potential characteristic metabolites and 13 characteristic proteins, to differentiate CBS honey from Apis cerana honeys produced in other regions. These findings provide foundational reference for the authentication and geographical origin traceability of CBS honey.
研究领域:
食品科学
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代谢组学
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蛋白质组学
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分析化学
·
产地溯源
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Wenmin Li,Yikun Zan,Tong Wu,Sa Yang,Linlin Liu,Shanshan Li,Pingli Dai,Jing Gao
Chlorantraniliprole (CAP), a diamide insecticide, is extensively applied to combat pests in various crops. However, the widespread use of insecticides has raised concerns about their potential impact on pollinators. In the present study, we explored the toxic effects of CAP in two important honey bee species, Apis mellifera and Apis cerana . The 48 h LC 50 values of CAP for A. mellifera and A. cerana was 256.052 mg/L and 109.709 mg/L, implying that A. cerana is more sensitive to CAP. Prolonged exposure to 40 mg/L CAP significantly impaired sucrose responsiveness and climbing activity in both bee species. Both species showed a decrease in GR activity and GSH content with increasing CAP concentration. By contrast, the activities of GST, CAT, P450 and NAD-MDH were increased in both A. mellifera and A. cerana , but the differences between the 10 mg/L and 40 mg/L treatments were less pronounced in A. mellifera . Moreover, the immune related genes exhibited differential responses to CAP when comparing the two species. Low CAP concentrations led to down-regulation in expression of toll but up-regulation in expression of apideacin and hymeopatecin in A. mellifera , whereas A. cerana exhibited minimal changes in these genes. Additionally, CAP significantly inhibited the expression of ER stress response genes gp - 93 and P58 in A. mellifera , while 10 mg/L of CAP promoted P58 expression in A. cerana . Our results highlight species-specific effects with the possible, distinct detoxification mechanisms and immune responses between A. mellifera and A. cerana . These findings serve as a foundation for further evaluating the safety of CAP for honey bee species and offer insights into the scientific use of pesticides.
研究领域:
昆虫学
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毒理学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2023
·
IF 2.9
Wu Jiao,Zhao Shan,Chen Xin,Jiu Yuanda,Liu Junfeng,Gao Jinglin,Wang Shijie
Honey quality is in relation to botanical origin, and physicochemical properties, elemental composition, and antioxidant activity have been used for assessment and identification of honeys. The goal of this study is to contribute to the general analysis of five unifloral honeys from Cocos nucifera L., Dalbergia benthami Prain, Bombax ceiba L., Castanea mollissima Bl., and mangrove in Hainan province, China. Our results revealed that B. ceiba honey had the highest pH (4.27), color (139.33 mm Pfund), ash content (1.03 g/100 g), and electrical conductivity (1312.00 μS/cm) in five unifloral honeys. Furthermore, B. ceiba honey also contained the highest levels of total phenolic content (75.54 mg GAE/100 g) and total flavonoid content (29.22 mg RE/100 g), as well as the strongest antioxidant activity (DPPH IC 50 value, 3.97 mg/mL; FRAP value, 6527.43 µmol TE/kg). Moreover, we revealed a considerable variation in element contents in honeys using ICP-MS, with potassium being the most predominant element. B. ceiba honey had the highest contents of K, Ca, Mg, and P, whereas the highest amount of Na was found in mangrove honey. Overall, our data indicated that B. ceiba honey deserves further research as a potential antioxidant agent.
研究领域:
农业
·
食品科学
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分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）