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PHYSIOLOGIA PLANTARUM · 2022 ·Vol. 174 ·期 2 ·e13650 · IF 5.081

Comparative profiles of the cuticular chemicals and transpiration barrier properties in various organs of Chinese flowering cabbage and Chinese kale

Hua Huang,Ying Hu,Ling Wang,Fengjun Li,Youxia Shan,Qiaoqiao Lian,Yueming Jiang

Plant cuticle as hydrophobic barrier covers almost all aerial plant organs. Herein the cuticular chemical components and the transpiration of various organs of Chinese flowering cabbage (CFC) and Chinese kale (CK) were comprehensively characterized. Numerous species- and organ-specific differences in morphological, chemical, and physiological levels were found. The various organs were relatively smooth in surface for CFC but glaucous with hollow tube- and plate-type crystals for CK. The chemical composition of cuticular waxes were very-long chain n -alkanes, ketones, secondary alcohols with a prominent carbon chain of C 29 in CK, primary alcohols dominated by C 26 , and aldehydes prominently C 30 in CFC. Cutin monomers accumulated with similar levels as waxes and were dominated by α , ω -dicarboxylic acids and fatty acids without added groups. The minimum water conductance differed considerably among species and various organs ranging between 8.9 × 10 −5 (CK leaf) and 3.7 × 10 −4 m s −1 (CFC leaf petiole). These differences in transpiration properties were proposed to be largely related to the cuticular chemicals in various organs and species. The presented results provide further insights to link the transpiration barrier functions with surface characteristics and cuticular chemicals.

CAS / MDL: 67-63-0 110-86-1
研究领域: 植物学 · 生理学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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Foods · 2023 ·Vol. 12 ·期 15 ·2843 · IF 5.2

The Screening and Isolation of Ethyl-Carbamate-Degrading Strains from Fermented Grains and Their Application in the Degradation of Ethyl Carbamate in Chinese Baijiu

Siyu Xue,Naihui Dong,Kexin Xiong,Hui Guo,Yiwei Dai,Huipeng Liang,Yingxi Chen,Xinping Lin,Beiwei Zhu,Sufang Zhang

Ethyl carbamate (EC), a 2A carcinogen produced during the fermentation of foods and beverages, primarily occurs in distilled spirits. Currently, most studies focus on strategies for EC mitigation. In the present research, we aimed to screen strains that can degrade EC directly. Here, we report twoCandida ethanolicastrains (J1 and J116), isolated from fermented grains, which can reduce EC concentrations directly. These two yeasts were grown using EC as the sole carbon source, and they grew well on different carbon sources. Notably, after immobilization with chitosan, the two strains degraded EC in Chinese Baijiu by 42.27% and 27.91% in 24 h (from 253.03 ± 9.89 to 146.07 ± 1.67 and 182.42 ± 5.05 μg/L, respectively), which was better than the performance of the non-immobilized strains. Furthermore, the volatile organic compound content, investigated using gas chromatography-mass spectrometry, did not affect the main flavor substances in Chinese Baijiu. Thus, the yeasts J1 and J116 may be potentially used for the treatment and commercialization of Chinese Baijiu.Keywords:ethyl carbamate (EC);Candida ethanolica;Chinese Baijiu;immobilization

CAS / MDL: 626-89-1
研究领域: 微生物学 · 食品科学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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Biocatalysis and Agricultural Biotechnology · 2024 · IF 3.4

Comparison of chemical components and quality evaluation of Panax ginseng and its processed products from different habitats in Northeastern China

Sethuraman Sathya,Jiarui Zhang,Huafang Feng,Pu Jing,Yu Lan,Xiaonan Cao,Qingqing Liu

Ginseng (Panax ginseng C.A. Meyer) is a widely used dietary supplement in Traditional Chinese Medicine due to its numerous health-promoting properties. However, limited research is available on the distinctions in the chemical composition between different ginsengs. Hence, this study explores the chemical composition of different ginsengs obtained from different habitats in Northeastern China. The fresh, red, and white ginsengs were collected from different places in the Jilin province, China. Ginsenoside and non-ginsenoside contents in the ginseng extracts were analyzed by an Ultra-high performance liquid chromatography system & VION® ion mobility hybrid Quadrupole Time-of-Flight. The results show a notable difference in the ginsenoside content among the samples. The qualification ratio of ginsenoside Re, Rg1, and Rb was 46.2%, with Re being the most abundant, followed by Rg1 and Rb1 in all the ginseng analyzed. Furthermore, the study revealed that white and red ginseng had higher total ginsenosides (8.18 ± 0.04 mg/kg) and total polysaccharides (17.4%) content, respectively. The fresh ginseng samples exhibited higher protein content (20.02 ± 0.2 g/100g) and lower fat content (2.0 g/100g) than others. Further, the ginseng samples analyzed in this study met the requirements specified in the Chinese Pharmacopoeia for total ash, heavy metals, and organochlorine pesticide residues. This study offers a comprehensive comparative analysis of the chemical composition of P. ginseng sourced from various habitats in Northeastern China. It underscores the significance of making informed choices when selecting ginseng types for scientific evaluation.

CAS / MDL: 64-17-5 50-99-7
研究领域: 药理学 · 化学 · 传统中药 · 植物学
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FOOD SCIENCE AND BIOTECHNOLOGY · 2016 ·Vol. 25 ·期 2 ·631-636 · IF 0.699

Are Chinese edible oils safe? A survey of trans fatty acid contents in Chinese edible oils

Huang Xiaojun,Nie Shaoping,Yang Meiyan,Xie Jianhua,Li Chang,Xie Mingyong

Nine different edible oil types from 7 cities in China were analyzed. Trans fatty acid (TFA) contents in edible oils were determined, TFA distribution characteristics were analyzed, and a risk assessment was performed. Total TFA contents varied from 0.075±0.011 to 2.243±0.770 g/100 g of oil, with a mean content of 0.66 g/100 g of oil. TFA contents differed among different oil types and among different cities. Generally speaking, oil samples from northern areas of China contained more TFA, and lard usually had higher levels of TFA than other oils. An exposure assessment showed that the estimated daily intake of TFA from Chinese edible oils was low, and a risk assessment indicated that there was a low risk for TFA consumption in Chinese edible oils.

研究领域: 营养学 · 食品安全 · 公共卫生
✓ 已验证 DOI PubMed 高影响因子
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JOURNAL OF HEALTH POPULATION AND NUTRITION · 2024 ·Vol. 43 ·期 1 ·1-9 · IF 2.4

The associations of fatty acids related dietary patterns with overweight and obesity among Chinese children

Huang Zhi,Ma Keyu,Yin Xiaochen,Li Ziming,Chen Ming,Duan Yujie,Li Li,Hu Yuming

Background Childhood overweight and obesity is becoming an emerging face of malnutrition. The aims of this study were to develop fatty acid (FAs) related dietary patterns and explored the associations of FAs related dietary patterns with overweight and obesity among Chinese children. Methods An observational study was conducted on 435 children aged 4 to 7 years old in South Central China. Erythrocyte FAs composition was analyzed by gas chromatography-mass spectrometry. Diet was collected by food frequency questionnaires and dietary patterns were evaluated by reduced rank regression. The logistic regression analysis was used to exploring the association of dietary patterns with overweight and obesity. Results The prevalence of overweight, obesity, and overweight or obesity were 6.52, 4.59, and 11.11% in Chinese children, respectively. Twenty five types of FAs were detected in erythrocyte of children and four FAs related dietary patterns were identified. The dietary pattern positively correlated with n-3 PUFAs, but negatively with SFAs,was characterized by high intake of fish, shrimp, crab and shellfish, leaf-off vegetable, nuts, and tubers, which have a significantly decreased overweight risk (OR = 0.580, 95%CI: 0.375 ∼ 0.895, P  = 0.014).The pattern positively strong associated with n-6 PUFAs, but negatively strong with n-3 PUFAs, had high intake of snacks, leaf-off vegetable, fresh beans, and coarse cereals, which have a significantly decreased obesity risk (OR = 0.518, 95%CI: 0.325 ∼ 0.827, P  = 0.006). Conclusion Four FAs related dietary patterns were identified. The dietary pattern with high intake of fish, shrimp, crab and shellfish decreased overweight risk by increasing n-3 PUFAs, and decreasing SFAs. The dietary pattern with high intake of plant food, decreased obesity risk by providing an balanced n-6/n-3 PUFAs ratio.

CAS / MDL: 1731-94-8
研究领域: 营养学 · 流行病学
✓ 已验证 产品关联 DOI PubMed 高影响因子
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FOOD SCIENCE AND BIOTECHNOLOGY · 2015 ·Vol. 24 ·期 3 ·1131-1138 · IF 0.653

Antioxidant and anti-inflammatory properties of Chinese ilicifolius vegetable (Acanthopanax trifoliatus (L) Merr) and its reference compounds

Wang Huaqian,Li Dongli,Du Zhiyun,Huang Mou-Tuan,Cui Xiaoxing,Lu Yujing,Li Chenyue,Woo Swee Lian,Conney Allan H.,Zheng Xi,Zhang Kun

Stems and leaves of Acanthopanax trifoliatus (L) Merr (AT) are commonly used as an edible vegetable in China. The scientific basis of antioxidant and antiinflammatory activities of AT was investigated. The polyphenol contents and free radical scavenging activities of AT ethanol extracts (EAT) were determined. Chlorogenic acid, isochlorogenic acid A, isochlorogenic acid C, and rutin were identified as major components in EAT. LPS-induced NO production in RAW264.7 cells was decreased significantly ( p <0.05) after EAT treatment, compared with controls. In vivo studies indicated that EAT had more activity than chlorogenic acid per mg of dry weight for inhibition of TPA-induced ear edema. TPA-induced increases in cytokine production (TNF-α, IL-6, MMP-9, and TIMP-1) were significantly ( p <0.05) reduced by EAT treatment, compared with controls. EAT exerts anti-inflammatory activities probably due, at least in part, to chlorogenic acid.

研究领域: 药理学 · 植物化学
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS · 2023 · IF 4.3

Formaldehyde levels and formaldehyde-exposure-related health risk levels of edible mushrooms cultivated in China

Yi Shao,Haiyan Liu,Liujuan Huang,Xiaoyan Zhao,Zhiyong Zhao,Xianli Yang,Changyan Zhou

This study aims to investigate the prevalence of naturally produced (NP) formaldehyde in edible mushrooms cultivated in China and the formaldehyde-exposure-related health risks of the mushrooms. Therefore, 2000 edible mushrooms samples including Lentinus edodes , Auricularia , Pleurotus ostreatus , Agaricus bisporus , Flammulina velutipes and Tremella fuciformis were collected from 21 provinces from 2013 to 2015. Then, their formaldehyde levels were analyzed by acetylacetone spectrophotometry , and the risks of formaldehyde exposure from the consumption mushrooms were calculated by point assessment and probability assessment using @RISK software. The results showed that, NP formaldehyde was common in farm-sourced (FS) samples, with the maximum level of 107 mg/kg in FS L. edodes . The formaldehyde levels in market-sourced (MS) fresh L. edodes samples were lower than those in FS L . edodes samples, and the formaldehyde levels in MS dried L . edodes samples ranged from 0 mg/kg to 270 mg/kg. The formaldehyde levels in fresh A . polytricha , P . ostreatus , A . bisporus and F . velutipes samples were not greater than 28 mg/kg, and those in dried A . auricula and T . fuciformis samples were not greater than 46 mg/kg. The results of point assessment and probability assessment indicated that, consuming the mushrooms posed no health risk to Chinese consumers.

CAS / MDL: 50-00-0
研究领域: 食品科学 · 毒理学 · 公共卫生
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Journal of Environmental Chemical Engineering · 2023 · IF 7.7

Chitosan immobilized Chinese gallotannin: A potential adsorbent to enrich the rare metals Ge (Ⅳ), Ga (Ⅲ), and In (Ⅲ)

Cheng Huang,Kun Li,Hong Zhang,Wenwen Zhang,Yanlin Sun,Shujie Xing,Ying Shao,Jing Zhu,Yalan Chen,Long Chen

In this study, a solid adsorbent based on Chinese gallotannin and chitosan was generated and applied to adsorb rare and scattered metals, including Ge (Ⅳ), Ga (Ⅲ), and In (Ⅲ), from simulated zinc plant leachates. Fourier-transform infrared spectroscopy (FTIR) and solid state nuclear magnetic resonance (SSNMR) were performed to characterize the grafting way of Chinese gallotannin on the chitosan matrix. It was clearly showed that Chinese gallotannin was immobilized on the chitosan by Schiff-base condensation and phenol-formaldehyde reaction at the molar ratio of glutaraldehyde to tannin of 2:1. And the amount of chitosan amino groups was ten times that of tannin, with an initial pH of 4. The adsorption experiments showed that an appropriate increase in pH was more beneficial to enhance the adsorption capacity of immobilized Chinese gallotannin (ICGT). At a temperature exceeding 30 °C, the immobilized tannin became less sensitive to temperature. Adsorption kinetics studies showed that the adsorption of the three scattered metals by ICGT followed a quasi-second-order kinetic equation , with chemisorption as the mechanism. The adsorption isotherm was well fitted by the Langmuir model, indicating uniform adsorption sites on the surface of ICGT. The adsorption capacities of Ge (Ⅳ), Ga (Ⅲ), and In (Ⅲ) in solution by the adsorption model of monolayer were 61.39 mg/g, 63.82 mg/g, and 59.92 mg/g, respectively. Mechanical property and stability tests showed that ICGT had good resistance to harsh adsorption conditions of scattered metal ions .

研究领域: 材料科学 · 环境科学 · 化学工程
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BIOLOGIA · 2023 · IF 1.5

Infestation and distribution of chiggers on Ryukyu mouse in southwest China

Guo Yu,Guo Xian-Guo,Song Wen-Yu,Lv Yan,Yin Peng-Wu,Jin Dao-Chao

Chiggers are a group of ectoparasites and they are the vector of scrub typhus. Although the Ryukyu mouse ( Mus caroli ) is a common species of rodent in some outdoor habitats, few studies are about the mouse itself and its ectoparasites. To date, there has been no specific report on chiggers of M. caroli . Based on the field investigation in southwest China from 2001 to 2019, this paper reported the infestation and distribution of chiggers on M. caroli for the first time. Among the 91 sites investigated, 263 Ryukyu mice were captured at 18 sites, and 678 chiggers were collected from the mice. The collected chiggers were identified to be one family, two subfamilies, six genera, and 16 species with relatively low species diversity. The overall infestation prevalence ( P M ), mean abundance ( MA ), and mean intensity ( MI ) were P M  = 27.38%, MA  = 2.58 chiggers/per mouse, and MI  = 9.42 chiggers/per mouse respectively. The dominant chigger species are Walchia ewingi and W. micropelta with a total constituent ratio of C r  = 94.10% (638/678), and they were of aggregated distribution among different individuals of the host M. caroli . The distribution of two dominant chigger species seemed to be mutually independent with the association coefficient V ≈ 0 ( p  < 0.001). The infestation ( P M , MA , and MI ) of M. caroli with chiggers greatly fluctuated in different geographical landscapes, altitudes, and latitudes with an obvious environmental heterogeneity. The male and adult M. caroli harbored much more chigger mites with significantly higher infestations ( P M , MA and MI ) than the female and juvenile mice. The theoretical curve of species abundance distribution of the chigger mite community on M. caroli was fitted by Preston lognormal distribution model with fitting goodness R 2  = 0.77. Based on the iChao 1 formula, the expected total number of chigger species on the mice was estimated to be 24 species.

CAS / MDL: 60-29-7
研究领域: 医学昆虫学 · 寄生虫学 · 生态学
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RENEWABLE ENERGY · 2021 · IF 8.001

Improving enzymatic saccharification of Chinese silvergrass by FeCl3-catalyzed γ-valerolactone/water pretreatment system

Junwei Yao,Xiaobao Xie,Qingshan Shi

In this work, we report that FeCl 3 , a benign Lewis acid, could be used as a catalyst in the γ-valerolactone (GVL)/H 2 O solvent system to facilitate efficient pretreatment of Chinese silvergrass. The results showed that approximately 87.8% of xylan and 75.8% of lignin were removed by 80% GVL under 170 °C using 50 mM FeCl 3 as a catalyst. The solubilized carbohydrate fractions in the pretreatment liquor were mainly composed of monosaccharides (glucose and xylose) rather than oligosaccharides (glucose oligomers and xylose oligomers) and inhibitors (furfural, hydroxymethylfurfural, formic acid and acetic acid). Moreover, FeCl 3 decreased the transesterification of GVL with hydroxyl groups on the cellulose surface and resulted in more easily degraded cellulose. Approximately 96.5% of cellulose and 92.2% of xylan in the pretreated Chinese silvergrass were converted to fermentable monosaccharides by 10 FPU/g cellulases . This study indicated that FeCl 3 -catalyzed GVL solvent could efficiently deconstruct biomass and increase the cellulose digestibility, which provided a novel, GVL-based pretreatment system for processing biomass.

CAS / MDL: 108-29-2
研究领域: 生物化学 · 可再生资源与环境科学
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ANTIMICROBIAL AGENTS AND CHEMOTHERAPY · 2020 ·Vol. 64 ·期 3 · IF 4.904

Omadacycline Efficacy against Enterococcus faecalis Isolated in China: In Vitro Activity, Heteroresistance, and Resistance Mechanisms

Zhiwei Lin,Zhangya Pu,Guangjian Xu,Bing Bai,Zhong Chen,Xiang Sun,Jinxin Zheng,Peiyu Li,Di Qu,Qiwen Deng,Zhijian Yu

This study aimed to evaluate the in vitro antimicrobial activity, heteroresistance emergence, and resistance mechanism of omadacycline (OMC) in clinical Enterococcus faecalis isolates from China. A total of 276 isolates were collected retrospectively in China from 2011 to 2015. The MICs of OMC, doxycycline (DOX), and minocycline (MIN) against E. faecalis were determined by broth microdilution. Tetracycline (TET)-specific resistance genes and multilocus sequence typing (MLST) of the isolates were investigated using PCR. The detection frequency of OMC heteroresistance in E. faecalis was evaluated with population analysis profiling (PAP). The mechanism of OMC heteroresistance and resistance in E. faecalis was examined by amplifying 30S ribosomal subunit genes, RNA sequencing (RNA-Seq), and in vitro recombination experiments. The OMC MICs of clinical E. faecalis isolates ranged from ≤0.06 to 1.0 mg/liter, and 42% of the E. faecalis isolates with an OMC MIC of 1.0 mg/liter were found to be sequence type 16 (ST16). Six OMC-heteroresistant isolates with MIC values of ≤0.5 mg/liter were detected among 238 E. faecalis isolates. The resistant subpopulations of heteroresistant isolates showed OMC MICs in the range of 2 to 4 mg/liter and were found without 30S ribosomal subunit gene mutations. Moreover, RNA sequencing and in vitro recombination experiments demonstrated that overexpression of a bone morphogenetic protein (BMP) family ATP-binding cassette (ABC) transporter substrate-binding protein, OG1RF_RS00630, facilitated OMC heteroresistance in E. faecalis. In conclusion, OMC exhibited better activity against clinical E. faecalis isolates from China than that of DOX or MIN, and overexpression of OG1RF_RS00630 in E. faecalis facilitated the development of OMC heteroresistance.

研究领域: 微生物学 · 感染病学 · 药理学
✓ 已验证 DOI PubMed 高影响因子
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FOOD CONTROL · 2012 · IF 2.656

Evaluation of the toxicity of the essential oils of some common Chinese spices against Liposcelis bostrychophila

Na Na Zhao,Ligang Zhou,Zhi Long Liu,Shu Shan Du,Zhi Wei Deng

Fourteen essential oils of common spices in China were evaluated for insecticidal activities against the booklouse, Liposcelis bostrychophila . Five essential oils ( Allium sativum , Cinnamomum cassia , Foeniculum vulgare, Illicium verum , and Perilla frutescens ) exhibited strong contact toxicity against the booklice at a concentration of 0.16   μL/cm 2 while only C. cassia , F . vulgare and P. frutescens essential oils possessed fumigant toxicity at a concentration of 0.04   μL/L. Based on bioactivity-directed fractionation, estragole, trans -anethole and fenchone were isolated from the essential oil of F . vulgare . Estragole and trans -anethole exhibited contact toxicity against the booklouse with LD 50 values of 49.95   μg/cm 2 and 57.98   μg/cm 2 , respectively while F . vulgare essential oil had an LD 50 value of 90.36   μg/cm 2 . trans -Anethole (LC 50   =   15.96   μg/L) possessed stronger fumigant toxicity against the booklouse than fenchone (LC 50   =   73.23   μg/L), estragole (LC 50   =   160.33   μg/L) and the essential oil of F . vulgare (LC 50   =   34.07   μg/L).

CAS / MDL: 62-73-7
研究领域: 昆虫学 · 植物化学
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