BIORESOURCE TECHNOLOGY
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2022
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IF 11.889
Wenqian Zhao,Xiaodong Zhang,Yanpeng Cai,Shan Zhao,Shuguang Wang
Metronidazole (MNZ), an antibiotic that is specifically used for the treatment of anaerobic infections, may inhibit anaerobic fermentation. This work was designed to understand the fate and effects of MNZ in mesophilic fermentation (MF) and thermophilic fermentation (TF), respectively. The results showed that the removal of MNZ mainly occurred via biodegradation, rather than adsorption, and that MNZ could be completely degraded by opening the imidazole ring. MFs were more strongly inhibited by MNZ than TFs. MNZ concentration increased from 0 to 25 mg/L, hydrogen yield (HY) decreased from 167.5 to 16.8 mL/g glucose (90.0% decrease), and butyrate yield almost completely disappeared in MFs, whereas in TFs, HY decreased only from 101.1 to 89.3 mL/g glucose (11.7% decrease), and ethanol yield increased by 39.8%. Illumina MiSeq sequencing analysis showed that MNZ reduced the abundance of hydrogen-producing bacteria. Furthermore, the inhibition of MNZ on anaerobic fermentation was reversible.
研究领域:
环境科学
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微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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2025
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IF 5.2
Sheng-Bing Yang,Zhen-Ming Lu,Han-Jun Shen,Jun-Jie Fu,Yang Yang,Xiao-Juan Zhang,Li-Juan Chai,Song-Tao Wang,Su-Yi Zhang,Cai-Hong Shen,Jin-Song Shi,Zheng-Hong Xu
Solid-state fermentation heterogeneity causes microenvironmental differences, shaping diverse microbial communities and metabolite compositions. Here, we aimed to investigate the variations in Qu -aroma between the inner (I-) and outer (O-) layers of medium-temperature Daqu (MT- Daqu ) starter, and to analyze the differences in microbial communities as well as the associated metabolic pathways related to Qu -aroma volatile organic compounds (VOCs). Firstly, quantitative descriptive analysis revealed that the I-layer exhibited intensified rancid-roasted notes, whereas the O-layer showed stronger grain and woody notes. Secondly, a total of 225 VOCs were identified from 88 inner and outer layer samples using HS-SPME-GC–MS, among which 43 differential VOCs were confirmed between the layers (VIP > 1, P < 0.05, and Fold change ≥1.5 or ≤0.67). Thin film (TF)-SPME-GC-O/MS revealed 52 and 47 odors with corresponding VOCs in the I- and O-layers, respectively. Subsequently, cross-referencing metagenomic and metabolic databases revealed that 37 aroma-active VOCs were functionally linked to six metabolic modules within the MT- Daqu microbial metabolic network. Finally, integrated metagenomic and amplicon sequencing identified potential functional microorganisms associated with specific metabolic modules. Five genera, including Kroppenstedtia and Thermomyces , were identified as characteristic of the I-layer, while 22 genera, such as Lactobacillus and Saccharomycopsis , were characteristic of the O-layer. Notably, Kroppenstedtia , Thermomyces , Lactobacillus , Saccharomycopsis , and Weissella were ubiquitously associated with all six metabolic modules across both layers of MT- Daqu . This study delivers a perspective for clarifying the spatial heterogeneity of Qu -aroma and its microbial drivers.
研究领域:
食品科学
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微生物学
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发酵技术
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分析化学
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代谢组学
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基因组学
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感官分析
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）