ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Yulin Zhai,Guoli Li,Kai Peng,Zhaosong Ge,Weibing Zhang,Duxin Li
The resolving power of multiple dimensional liquid chromatography (mD-LC) is multiplicative as it adds dimensions. However, the issue in creating a preparative mD-LC system is that the higher the dimensionality, the more complicated the system configuration. Thus, we presented a new configuration of preparative mD-LC using one set of LC modules and trapping array-based multiple heart-cut interfaces. A preparative two-dimensional liquid chromatography (2D-LC) separation of herbal medicine formulation produced 40 compounds with a purity of >90%. During the separation process, the interface stores the fractions and allocates positions for the fractions from a different dimension; LC draws the fraction from the interface, makes nD separation, and sends isolated fractions to the interface. By repeating this process, we achieved variable dimensionality of LC separations. We also presented a preparative 3D-LC separation of herbal medicines to validate the principle of “less configuration and more dimensionality”. Thus, we can explore the higher dimensional preparative separations. The developed preparative mD-LC displayed exceptional power in the isolation of various compounds and has great potential in the application of natural products.
研究领域:
分析化学
·
天然产物化学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Luwei Fang,Minglei Tian,Xuemin Yan,Wei Xiao,Kyung Ho Row
A convenient, and efficient multi-phase extraction method was used to isolate aristolochic acid from real samples. To increase the efficiency, a new dual ionic liquid-immobilized silica was used as a sorbent and the effects of the adsorption conditions were investigated. IM-BIM@Sil in a methanol/water (60:40, v/v) solution at 60 °C extracted the highest amount of aristolochic acid (16.69 mg/g) compared to the other sorbents examined. The sorbent was then applied to the multi-phase extraction of aristolochic acid. After extraction eight times, 2.4–70.9 × 10 −3 mg/g of aristolochic acid was isolated from nine real samples after several washing and elution steps. The recoveries of aristolochic acid ranged from 70.0% to 110.6% with relative standard deviations of 3.5%–9.1%, highlighting the accuracy of this method.
研究领域:
分析化学
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药物学
·
植物化学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yue Zhang,Weibiao Wang,Mei Wang,Lijuan Ma,Weiman Zhang,Shuxian Zhang,Gidion Wilson,Zhigang Yang,Yuping Sa,Fen Ma,Xinmin He,Tao Gao,Hui Yuan,Guoning Chen,Xueqin Ma
β-Lactamases (β-LS) are a significant threat to public health due to their role in conferring antibiotic resistance against nearly all clinically used β-lactam antibiotics. Consequently, the search for β-lactamase inhibitors (β-LSI), which can reversibly bind to and inactivate β-LS, has intensified. Here, a sensitive and rapid cellulose-based colorimetric sensor integrated with affinity identification model was designed for high-throughput screening of β-LSI. The innovative concept of a nitrocefin-based colorimetric paper sensor strategy was effective in rapidly detecting the inhibitory effects of herbals on β-LS. Then, potential β-LSI present in the screened herbals were captured, separated, and identified utilizing affinity identification model. The efficacy of the developed method was validated with both negative and positive drugs and was subsequently applied to the screening of β-LSI in 7 different herbal samples. Notably, Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou exhibited significant β-LS inhibitory activity. Further affinity separation and LC-MS analyses identified 2 bioactive compounds, spinosin, and magnoflorine, as the agents responsible for this inhibitory effect, with IC 50 values of 143.53 μM and 294.66 μM, respectively. The inhibitory effects and mechanisms were validated through antibacterial activity testing, molecular docking, and dynamic simulation. Notably, spinosin combined with aztreonam showed greater antibacterial effectiveness than avibactam. This study not only establishes a platform for the effective preliminary screening of β-LSI as a potential antimicrobial resistance-reversing agent for future clinical applications, but also inspires the further development of other cellulose-based colorimetric sensors for the rapid and high-throughput discovery of various enzyme inhibitors in complex natural medicines.
研究领域:
微生物学
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药理学
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生物化学
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产品关联
DOI
高影响因子
Frontiers in Veterinary Science
·
2025
·
IF 2.9
Jie Luo,Yiping Wang,Wenya Zheng,Chaolong Xu,Jianhua Chen,Ben Liu,Xiaoyue Wang,Manxin Fang
Introduction Mastitis outbreaks in dairy goats at a farm in western Jiangxi Province, have recently imposed significant economic losses on farmers. This study aimed to identify the causative pathogen and implement targeted control measures for early disease eradication. Methods Pathological autopsy was performed on diseased goats submitted for examination. Pathological mammary gland tissues and milk cistern fluid were collected aseptically for bacterial isolation and purification. The isolated pathogen was characterized through colony morphology observation, Gram staining, biochemical tests, and 16S rDNA gene sequencing. Pathogenicity was assessed using an animal challenge test in mice. In vitro antibacterial susceptibility testing was conducted using 30 Antibiotics (e.g., cefradine, cephalexin, cefuroxime), 18 Chinese herbal medicines (CHMs; e.g., Rhus chinensis (Galla Chinensis), Coptis chinensis (Chinese Goldthread), Punica granatum (Granati Pericarpium)), and 15 phytogenic extracts (e.g., Berberine, Gallic acid, Tannic acid). Results A Gram-positive bacterium was isolated from the diseased tissues. Biochemical profiling and 16S rDNA sequencing identified the isolate as Staphylococcus caprae . The animal challenge test confirmed the isolate’s strong pathogenicity in mice. Susceptibility testing indicated that the isolate was susceptible to Antibiotics such as cefradine, cephalexin, cefuroxime, and ciprofloxacin, while showing the poorest susceptibility to penicillin among the tested antibiotics. Among the CHMs and phytogenic extracts, Rhus chinensis (Galla Chinensis), Coptis chinensis (Chinese Goldthread), Punica granatum (Granati Pericarpium), Berberine, Gallic acid and Tannic acid demonstrated superior antibacterial efficacy. Discussion This study provides multiple therapeutic and preventive options (CHMs, Antibiotics, phytogenic extracts) for mastitis control on this farm. It also offers valuable insights into the identification and treatment of mastitis in dairy goats, contributing to safeguarding goat health and ensuring dairy product safety.
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DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 40
·期 17
·3522-3534
·
IF 2.557
Xiaoli Gu,Jiaojiao Huang,Lei Zhang,Yu Zhang,Chong-Zhi Wang,Chenghong Sun,Dandan Yao,Fei Li,Lina Chen,Chun-Su Yuan
In the scope of stroke treatment, new neuronal nitric oxide synthase–postsynaptic density protein-95 uncouplers from herbal medicines were discovered and captured. To do so, highly selective magnetic molecularly imprinted polymers with a core–shell structure were prepared as artificial antibodies. According to the results of computational simulations, we designed and synthesized various polymers with varying amounts and types of template, functional monomer, cross-linker, and solvent. Characterization and performance tests revealed that the most appropriate artificial antibodies showed uniform spherical morphologies, large adsorption capacities, fast-binding kinetics, high selectivity, and quick separation. These artificial antibodies were then used as sorbents for dispersive magnetic solid-phase extraction coupled with high-performance liquid chromatography and mass spectrometry to capture and identify structural analogs to ZL006 from extracts of Scutellariae radix, Psoraleae fructus, and Trifolium pratense. Furthermore, according to the neuroprotective effect and coimmunoprecipitation test, Baicalein, Neobavaisoflavone, Corylifol A, and Biochanin A can be the potential uncouplers of neuronal nitric oxide synthase–postsynaptic density protein-95. Therefore, this present study contributes valuable information for the discovery of neuronal nitric oxide synthase–postsynaptic density protein-95 uncouplers from herbal medicines.
研究领域:
药理学
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神经科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2019
·Vol. 31
·期 7
·1390-1400
·
IF 2.691
Ying Wang,Xamxikamar Mamat,Yongtao Li,Xun Hu,Ping Wang,Yemin Dong,Guangzhi Hu
In this study, a molybdenum disulfide/multiwalled carbon nanotubes (MoS 2 @MWCNTs) nanocomposite was synthesized by employing a simple hydrothermal method. The flower-like structure of the MoS 2 @MWCNTs was characterized via scanning electron microscopy (SEM) and transmission electron microscopy (TEM), and the load of crystalline MoS 2 was verified via X-ray diffraction (XRD) and energy-dispersive spectroscopy (EDS). The as-prepared MoS 2 @MWCNTs nanocomposite was used to modify glassy carbon electrode (GCE) as an electrochemical sensor for detecting aristolochic acids (AAs). With the optimized parameters, the proposed electrochemical sensor exhibited good sensitivity and a broad linear concentration range for detecting AAs from 0.2 to 10 μ mol/L and 10 to 100 μ mol/L, with the sensitivity of −3.10 μ A/( μ mol/L) and −0.91 μ A/( μ mol/L), respectively. The detection limit was also calculated as 0.06 μ mol/L (S/N=3) based on the low background signal. Furthermore, the modified electrochemical sensor exhibited good selectivity, repeatability, reproducibility, and stability, thus showing potential application for detecting AA in chinese herbs with good mean recovery and accuracy. In other words, the MoS 2 @MWCNTs/GCE can be used as an excellent platform to detect AAs.
研究领域:
分析化学
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纳米材料
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电化学
·
药物分析
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）