JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Jinlai Cheng,Xiaoyu Zhang,Yuyan Xu,Yu Zhao,Guozhuang Zhang,Gejing De,Yuqing Tan,Huajing Wang,Miyi Yang
A thermo-responsive magnetic molecularly imprinted polymer (TMMIP) based on magnetic graphene oxide (MGO) was prepared via surface molecular imprinting technique for selective recognition of sinomenine (SIN) in various complex matrices. The obtained nanoparticles were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), vibrating sample magnetometry (VSM), and thermogravimetric analysis (TGA). The results indicated that the TMMIP exhibited a typical core-shell structure, comprising a molecularly imprinted shell, and demonstrated both magnetic susceptibility and excellent stability. The adsorption performance of TMMIP was also studied via adsorption kinetics, adsorption isotherms and selectivity experiments. The TMMIP exhibited high adsorption capacity, high selectivity for SIN, and mild acidic elution conditions. Under the optimal conditions, an analytical methodology combining TMMIP based dispersive solid-phase microextraction with high-performance liquid chromatography (HPLC) was subsequently established. TMMIP can rapidly adsorb SIN in solution and easily collected with assistance of outer magnetic field. The analysis method exhibited linearity across 0.05-150 μg/mL with a correlation coefficient of 0.9993, while demonstrating detection sensitivity at 0.1 ng/mL (DL) and quantification capability at 0.3 ng/mL (QL). Validation using Caulis sinomenii , oral liquid and blood samples acquired recoveries of 88.8–110.4%. This integrated approach demonstrates high efficiency for SIN extraction and detection in complex matrices and provided an efficient, eco-friendly strategy for SIN analysis.
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高影响因子
DIAMOND AND RELATED MATERIALS
·
2024
·
IF 4.3
YuXuan Xiong,Dandan Luo,Guowen He,Saiwen Liu,Jin Zhang,Chao Chen
Baicalin is a principal component of traditional Chinese medicine and plays a crucial role in treating conditions such as arthritis, respiratory tract infections, chronic and acute hepatitis, and tumors. Graphene oxide is bonded to silicon dioxide (SiO 2 ) through covalent bonds or hydrogen bonds and then reduced to SiO 2 @rGO through hydrazine hydrate to solve the problem of easy accumulation of SiO 2 and improve the conductivity of the material. Next, β-cyclodextrin (β-CD) self-assembles on SiO 2 @rGO to form a stable inclusion complex. The hydrophobic inner cavity of β-CD can effectively accommodate various guest molecules to form inclusion complexes, enhancing molecular recognition and detection capabilities. Finally, β-CD/SiO 2 @rGO is bridged and surface-loaded between clay-like Ti 3 C 2 T x (MXenes) nanosheets through electrostatic interactions to form a stable three-dimensional composite material. It is precisely because of MXenes, due to their unique layered microstructure, excellent conductivity, and extensive hydrophilic surface area, that they are conducive to self-assembly or embedding nanoparticles in aqueous solution. The composite was characterized via X-ray diffraction, X-ray photoelectron spectroscopy, and scanning electron microscopy. Under optimized conditions, the electrochemical sensor constructed from this composite exhibited a broad detection range (0.008–10 μM) and a low detection limit (0.191 nM) for baicalin. In comparison to existing methods for detecting baicalin, this approach demonstrates strong anti-interference capabilities and stability. It can efficiently, sensitively, and accurately quantify baicalin in Chinese patent medicine samples, including Scutellaria baicalensis root, Shuanghuanglian oral liquid, and Bear Bile Scutellaria baicalensis eye drops, achieving a satisfactory recovery rate. This study introduces a novel approach for the electrochemical detection of baicalin.
研究领域:
材料科学
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中药分析
·
生物医学工程
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Yikun Liu,Wei Zhou,Zhenkun Mao,Zilin Chen
Evodiae Fructus is used as a traditional Chinese medicine for the treatment of several kinds of diseases with its bioactive constituents. In this study, a capillary electrochromatography-mass spectrometry (CEC-MS) method was developed to determine three bioactive compounds including evodiamine, rutaecarpine and limonin in Evodiae Fructus fruit. Home-developed monolithic columns with methyl-vinylimidazole functionalized organic polymer monolilth as stationary phases were used in CEC-MS with excellent separation selectivity and high efficiency. The CEC-MS methods provided 4–16 folds improvement of LODs when compared with CEC-UV method. The conditions, which could affect separation efficiency and detection sensitivity, were optimized. Under optimum conditions, baseline separation with high detection sensitivity was obtained. The method showed good linearity (R 2 >0.99) of 0.8–160 μg mL −1 with low limits of detection of 0.15-0.31 μg mL −1 . Relative standard deviations of migration time and relative peak areas were <13.89%. Recoveries of evodiamine, rutaecarpine and limonin in Evodiae Fructus fruit were tested and calculated, which ranged from 102% to 113%. Finally, the three bioactive compounds in Evodiae Fructus herb samples from different regions were analyzed and studied. It has been demonstrated that the developed method has great potential for quality control of Evodiae Fructus herb.
研究领域:
中药分析
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分析化学
·
药学
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高影响因子
Current Analytical Chemistry
·
2017
·Vol. 13
·期 3
·250-255
·
IF 1.306
Luan Feng,Lu Yuxi,Tang Lili,Liu Huitao
Background: The safety and level of heavy metal ions in the traditional Chinese medicine has become an important topic. Most of the metal ions are carcinogens and lead to serious health concerns by producing free radicals. It is of great significance to develop simple and effective
methods to determine heavy metal ions. The aim of this paper is to develop a CE-indirect UV method for the analysis of the four heavy metal ions for Chinese medicine Shuanghuanglian oral liquid. Methods: The alternative CE method for separation and test of metal ions was developed in
this paper, which can help control the safety and level of heavy metal ions in traditional Chinese medicine Shuanghuanglian oral liquid. And the developed HPCE method showed superior analytical performance, including high sensitivity and good selectivity. Results: The experimental conditions
of the CE separation method were: 5s hydrodynamic injection at 0.5 psi, 20 kV applied voltage, and UV detection at 218 nm. The results show that the migration time of all the four heavy metal ions was less than 6 min. The calibration curves for four heavy metal ions were linear in the range
of 0.5-35 μg mL-1 with linear correlation coefficients of 0.9984-0.9999. The limits of detection were in the range of 0.08-0.61 μg mL-1 along with the recoveries of 93.0% to 109.5%. Conclusion: A simple, rapid and sensitive HPCE method has been developed to separate four heavy metal
ions including Cd2+, Cr3+, Cu2+ and Zn2+ in traditional Chinese medicine Shuanghuanglian oral liquid. The comprehensive analysis performance is satisfactory. The proposed HPCE method provides shorter analysis time and lower detection limit. The results demonstrated that the developed CE method
can be potentially applied as an good alternative for quality control of TCM.
研究领域:
中药分析
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药物安全
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高影响因子
Journal of Analytical Methods in Chemistry
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2017
·
IF 1.801
Kai Hu,Yonghui Qiao,Zhifen Deng,Mingxia Wu,Wei Liu
The five anthraquinones compounds (including aloe-emodin, emodin, physcion, chrysophanol, and rhein) are regarded as the main effective ingredients in rhubarb (Dahuang in Chinese, one of the commonly used Chinese herbal medicines). In this work, a simple and effective solid phase extraction (SPE) method based on bis(tetraoxacalix[2]arene[2]triazine) modified silica gel as adsorbent was developed. Coupled with UHPLC-FLD, the developed method was successfully applied for the measuring of main anthraquinones in human urine after oral administration of the extracts of rhubarb. To obtain the highest recoveries of the five anthraquinones in the SPE process, the main parameters which may affect extraction efficiency were optimized. The optimized sorbent amount, sample loading pH, sample loading rate, washing solution, and eluent condition were obtained. The developed method showed good linearity in 0.012–1.800 μg mL−1 for the five anthraquinones with correlation coefficients more than 0.9993. The investigated LOD values ranged from 3.9 to 5.7 ng mL−1, while the LOQs were between 12.0 and 18.2 ng mL−1. The recoveries of the method were also investigated, which were in the range of 94.8–106.6%. The application of the mixed-mode SPE materials in the proposed method was feasible and simple, and suitable for the enrichment of anthraquinones in urine samples.
研究领域:
中药分析
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色谱技术
·
药物代谢研究
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PubMed
高影响因子
International Journal of Electrochemical Science
·
2024
·
IF 1.3
Ying Wang,Wei He
This study presents a novel electrochemical fingerprinting method for Buzhong Yiqi Tang extract granules using a diamond-graphene composite electrode. The electrode was fabricated through chemical vapor deposition of graphene on high-pressure high-temperature synthetic diamond, resulting in a few-layer graphene coating as confirmed by Raman spectroscopy (I₂ D /I G ratio ≈ 1.2) and XPS analysis (78 % sp² C-C signal). Cyclic voltammetry revealed four anodic and three cathodic peaks, while differential pulse voltammetry provided enhanced resolution with eight distinct oxidation peaks. The method demonstrated high sensitivity (LODs 0.08–0.15 mg/mL) and good linearity (R² > 0.995) over a concentration range of 0.5–10 mg/mL. Comparison of fingerprints from individual herb extracts (Astragalus membranaceus, Panax ginseng, and Glycyrrhiza uralensis) elucidated the origins of major peaks in the Buzhong Yiqi Tang profile. Principal component analysis of fingerprints successfully differentiated between samples from three manufacturers, accounting for 87.3 % of total variance. Strong correlations (r > 0.92) were observed between key DPV peak intensities and HPLC-determined concentrations of traditional chemical markers. The rapid analysis time, minimal sample preparation, and holistic representation of multiple electroactive components make this approach a promising complement to existing chromatographic methods for quality assessment of traditional Chinese medicine formulations.
研究领域:
中药分析
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电化学
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高影响因子
MICROCHEMICAL JOURNAL
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2024
·
IF 4.9
Shuanglin Deng,Fang Wang,Meng Xiong,Bangxing Han,Hui Yan
The rapid, accurate, and on-site quantitative evaluation of the 3 components (total polysaccharide, total flavonoid, and total saponin) of Polygonatum cyrtonema Hua (PCH) is of great significance to ensure the value of its medicine and food, for this purpose, a handheld near-infrared (NIR) spectrometer was used. The raw NIR spectra were preprocessed by seven methods and their combinations, then three kinds of wavelength selection algorithms, the competitive adaptive reweighted sampling (CARS), random frog (RF) sampling, and Monte Carlo uninformative variable elimination (MCUVE) were applied, and the characteristic wavelengths for total polysaccharide, total saponin, and total flavonoid in PCH were obtained, respectively. Subsequently, the partial least squares (PLS) regression models were developed. The results showed that the combination of first derivative and standard normal variate was the optimal pretreatment method, and CARS had the highest performance of the wavelength selection for total polysaccharide and total flavonoid, while RF was the highest for total saponin. With these selected wavelengths, 3 PLS prediction models were built, and their performances were validated with unknown samples, the results showed that the determination coefficient of the prediction set (R 2 P ) of total polysaccharide, total saponin, and total flavonoid were 0.980, 0.948, and 0.942, respectively, and the corresponding root mean square error of prediction set (RMSEP) were 0.162, 0.142, and 0.052. Furthermore, the residual prediction deviations (RPDs) of the three components were all larger than 3, demonstrating the prediction performance of the models was high. Therefore, the three active functional components in PCH could be rapidly determined by a handheld NIR spectrometer, which would be beneficial for ensuring the quality of PCH.
研究领域:
中药分析
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光谱学
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高影响因子
Advanced Composites and Hybrid Materials
·
2024
·Vol. 7
·期 6
·1-16
·
IF 23.2
Jiang Youwei,Sima Yingyu,Alomar Taghrid S.,AlMasoud Najla,El-Bahy Zeinhom M.,Feng Jinxia,Wan Kun,Toktarbay Zhexenbek,He Quanguo,Ding Jianhua,Wang Jingfeng,Wang Wu
The pursuit of rapid and sensitive detection methods for bioactive compounds in traditional Chinese medicine (TCM) is a continuous quest in the field of natural product chemistry. Herein, we report the development of a novel nanocomposite material comprising bismuth molybdate (Bi 2 MoO 6 ) and multi-walled carbon nanotubes (MWCNTs), which has been successfully applied to the ultrasensitive and simultaneous detection of the bioactive flavonoids baicalein and chrysin. These compounds, known for their potent anticancer and antioxidant properties, are prevalent in TCM and nutraceuticals. The Bi 2 MoO 6 -MWCNTs nanocomposite was meticulously characterized using X-ray diffraction (XRD), scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS), and cyclic voltammetry (CV), revealing its structural and functional attributes. The optimized electrochemical sensor, based on a glassy carbon electrode modified with the nanocomposite, demonstrated remarkable sensitivity with a linear response range of 0.001–5.0 μmol·L −1 for baicalein and 0.007–3.0 μmol·L −1 for chrysin, alongside impressively low detection limits of 0.2 nmol·L −1 and 1.4 nmol·L −1 , respectively. The sensor’s performance was further validated through its application in the analysis of human serum and Scutellaria baicalensis extracts, showcasing its robust repeatability, selectivity, and stability. This work not only advances the field of TCM analysis but also opens new horizons for the integration of hollow bimetallic oxides with carbon-based materials in electrochemical sensing, offering a strategic approach to the rapid assessment of bioactive constituents in complex matrices.
研究领域:
中药分析
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材料科学
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生物医学工程
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Tingfan Tang,Xuesong Li,Ziwei Xu,Jiayu Zhao,Yaqin Zhan,Yixing Li,Lijun Li,Hao Cheng,Yuhong Tian,Wenyi Huang,Jun Feng
In this study, magnetic multi-template molecularly imprinted polymers (MMIPs) are successfully synthesized. The synthesis is carried out by a multi-template surface imprinting strategy using mesoporous silica-coated magnetic graphene oxide as the support material, matrine (MT), oxymatrine (OMT) and sophocarpine (SC) as the templates, 4-vinylbenzoic acid as the functional monomer, and ethylene glycol dimethacrylate (EDGMA) as the cross-linker. The MMIPs were characterized using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The maximum adsorption capacity of the prepared MMIPs was found to be 9.01, 20.87, and 7.76 mg/g for MT, OMT, and SC, respectively, demonstrating higher adsorption capacity compared to the non-imprinted polymers (MNIPs). Furthermore, MMIPs were utilized as adsorbents for magnetic solid-phase extraction (MSPE) in conjunction with pressurized capillary electrochromatography (pCEC) for the simultaneous separation and detection of MT, OMT, and SC in the Chinese herbal medicine substrate sophora. The factors affecting the extraction efficiency of MSPE, including the type of elution solvent, amount of adsorbent, pH of the sample solution, elution time, and eluent volume, were optimized. The MMIPs-pCEC method demonstrated a satisfactory linear response within the concentration range of 1–50 μg/mL for the three alkaloids, with correlation coefficients ranging from 0.9923 to 0.9972 and limits of detection ranging from 0.09 to 0.73 μg/mL. The findings of the present study demonstrate that MMIPs have important applications in the analysis of active ingredients in traditional Chinese medicine.
研究领域:
中药分析
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材料科学
·
分析化学
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PubMed
高影响因子
Journal of Nanoelectronics and Optoelectronics
·
2022
·Vol. 17
·期 1
·22-29
·
IF 0.697
Meng Lifen,Lv Jingsong,Li Qianzhu,Tan Ye,Xia Zhi,Wu Haizhi
In this study, trace amounts of heavy metal ions copper, lead, mercury and cobalt in Pinellia ternata samples were detected by ionic liquid ultrasonic assisted dispersion liquid–liquid microextraction, combined with high performance liquid chromatography-ultraviolet detector.
The method used sodium diethyldithiocarbamate as complexing agent and phosphoric acid ionic liquid Trihexyl(tetradecyl) phosphine bis[(2,4,4-trimethyl)] amyl phosphonate (cyclophosphate IL104) as extractant. Methanol: 0.2% phosphoric acid (60:40) was used as mobile phase for separation and
UV detector was used for detection, the flow rate was 1 mL/min and the injection volume was 10 μL. In this paper, the effects of sample pH, complexing agent concentration, ultrasonic time, centrifugation time and speed and ionic strength were optimized. Under the optimal conditions
(50 μL ionic liquid cyclic phosphine IL104, pH = 5, 0.5% sodium diethyldithiocarbamate, ultrasonic time of 30 min, centrifugation speed of 4000 r/min, 0.1 g NaCl), the recovery of spiked standard was 89.0~98.2%. High performance liquid chromatography had the characteristics of low
detection limit, accurate and reliable determination results, high sensitivity, strong specificity and good reproducibility.
研究领域:
中药分析
·
环境毒理学
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高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2011
·
IF 2.733
Lihong Liu,Shenglan Li,Zilin Chen
A simple and selective method was developed for the simultaneous determination of tetrandrine and fangchinoline in herbal medicine by HPLC with electrochemical detection (ECD) on a bare glassy carbon electrode. The HPLC separation and ECD conditions have been optimized. The separation was carried out on a WondaSil ® C18-WR column (4.6 mm × 250 mm, 5 μm), with the mobile phase of acetonitrile–ammonium acetate buffer (pH 6.5; 40 mM) (32:68, v/v) using an isocratic elution at the flow rate of 0.5 mL/min. The electrochemical detection potential was set at +0.9 V. The obtained LODs for tetrandrine and fangchinoline were 0.26 and 0.27 μmol/L, respectively. The method was successfully applied to the analysis of tetrandrine and fangchinoline contents in Stephania tetrandra S. Moore. It has been demonstrated that the LC-ECD method is an excellent technique for analysis of the herbal medicine. The mean recoveries were in the range of 95–105%, while the precision expressed as repetition of peak area was lower than 2.7%.
研究领域:
中药分析
·
植物化学
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DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2016
·
IF 2.822
Wenjing Wang,Lu Wang,Lina Zou,Gaiping Li,Baoxian Ye
Arctigenin was determinated by an electrochemical method for the first time based on a novel voltammetric sensor, which was prepared by simple dipping-drying of SWCNTs on glassy carbon electrode (GCE) initially and then electrochemical deposition of Iodide. The resulting Iodide/SWCNTs/GCE displayed a significant voltammetric response to arctigenin due to the electrocatalytic ability of Iodide for arctigenin and a large specific surface area, excellent electric conductivity of SWCNTs. Meanwhile, the redox mechanism of arctigenin on Iodide/SWCNTs/GCE was also studied. The wide linear range of 3 × 10 − 8 mol L − 1 to 5 × 10 − 6 mol L − 1 and low detection limit of 1 × 10 − 8 mol L − 1 for the determination of arctigenin were achieved together with high sensitivity, selectivity, good stability. To examine the practical analytical utility of the proposed electrode, the real sample Fructus Arctii was examined and the result was found satisfactory.
研究领域:
分析化学
·
电化学传感器
·
中药分析
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高影响因子
危险品化学品经营许可证（带存储）