MOLECULES
·
2022
·Vol. 27
·期 21
·7636
·
IF 4.927
Xuyan Fan,Tao Tang,Song Du,Ningning Sang,Hao Huang,Chenghui Zhang,Xueping Zhao
A rapid, efficient, simple, and high-throughput method for the simultaneous determination of 108 pesticide residues in three traditional Chinese medicines (TCMs) was established, comprising an improved QuEChERS method in combination with HPLC-MS/MS based on mixed samples. A quantity of 10 mL of acetonitrile was used as extraction solvent, and 10 mg of amino-modified multi-walled carbon nanotubes (MWCNTs-NH2) and 150 mg of anhydrous magnesium sulfate (MgSO4) were selected as sorbents for dispersive solid phase extraction. The performance of the method was verified according to the analytical quality control standards of SANTE/11813/2017 guidelines. With good linearity (R2> 0.9984) in the range of 2–200 μg/L for all pesticides in the selected matrices, and good accuracy, precision, and high sensitivity, the recoveries were in the range of 70–120% for more than 95% of the pesticides, with a relative standard deviation (RSD) of less than 16.82% for all. The limit of detection (LOD) and limit of quantification (LOQ) of the method were 0.01–3.87 μg/kg and 0.07–12.90 μg/kg, respectively, forFritillaria thunbergiiMiq (F. thunbergii),Chrysanthemum MorifoliumRamat (C. morifolium), andDendrobium officinaleKimura et Migo (D. officinale). The method was successfully applied to 60 batches of actual samples from different regions.Keywords:pesticide residue;QuEChERS;MWCNTs-NH2;traditional Chinese medicines
研究领域:
分析化学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Separation Science Plus
·
2019
·Vol. 2
·期 10
·384-391
·
IF 0
Zhongping Huang,Xiaoli Huang,Huijun Liu,Lili Wang,Yilei Fan,Charles Liu,Yiping Ren
A new method of direct analysis in real-time integrated with mass spectrometry was established and applied to the rapid determination of ferulic acid in Chinese herbs. Instrument parameter settings were optimized to obtain the sensitive and accurate determination of ferulic acid. At the sample introduction speed of 0.5 mm/s, high signal intensity of [M-H] − ( m/z 193) for ferulic acid was achieved in the negative ion mode by utilization of high purity helium gas at 350°C. The calibration curve was linear over the range of 5 – 100 mg/L with the correlation coefficient r = 0.9846 and relative standard deviation (n = 6) in the range of 2.0 to 6.1%. The limit of detection ( S/N = 3) and limit of quantitation ( S/N = 10) were 0.4 and 1.2 mg/L respectively. The recoveries were in the range of 90.6–114.2%. The results of direct analysis in real time-mass spectrometry were in agreement with those obtained by high performance liquid chromatography. The proposed direct analysis in real time mass spectrometry method is a simple, rapid and high-throughput approach for the determination of ferulic acid in Chinese herbs.
研究领域:
中药学
·
分析化学
✓ 已验证
DOI
Biomed Research International
·
2015
·
IF 1.579
Wen-Hao Yu,Na Zhang,Jin-Feng Qi,Chen Sun,Yong-Hui Wang,Mei Lin
Toxic heavy metals, including mercury (Hg) and arsenic (As), accumulate preferentially in kidneys and always cause acute renal failure. The aim of this study was to investigate whether these samples affect organic anion transporters, Oat1 and Oat3, in vivo in mice kidney. Mice () were orally treated with investigational samples. After last administration, all mice were i.v. p-aminohippuric acid (PAH), and the blood and kidneys samples were collected. The concentrations of PAH were quantified by spectrophotometry. mRNA expressions of Oat1 and Oat3 were assayed by real-time PCR. In comparison with corresponding control, major pharmacokinetic parameters of PAH in sera were significantly changed by investigational samples (), PAH accumulations in the kidney tissues were significantly higher (), PAH uptake by renal slices was greatly reduced, Oat1 and Oat3 mRNA expression were significantly inhibited in investigational sample groups. Arsenic and mercury containing traditional Chinese medicine (Realgar and Cinnabar) probably induce kidney damage through inhibiting several members of the organic anion transporters (such as OAT1 and OAT3).
研究领域:
药理学
·
毒理学
·
肾脏病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2021
·
IF 2.495
Yinyin Wang,Yun Ai,Yilin Zhang,Yuanyuan Ren,Jilin Wang,Fangyuan Yao,Wenyu Li,Ying Zhou,Yanni Sun,Jianli Liu,Cuiling Wang
A new probe TR-V (Indolo[2,1–b]quinazoline-6,12-dione,6-{(6Z),(2′E)-2′- [(2-hydroxy-3-methoxyphenyl)methylene]hydra zinylidene}) based on tryptanthrin has been synthesized for selective and rapid detection of Cu 2+ . The synthetic route is facile, economical and viable. TR-V reacts in Tris-HCl buffer solution (pH = 8.0) with cupric to a deep yellow chelate which has a significant peak at 450 nm in the UV–Vis spectra. The reaction is instantaneous, ranging from light yellow to dark yellow and the change in color can be observed with the naked eye. Over 13 competitive metal ions do not interfere in the determination. The detection limit is 84 nM (R 2 = 0.9998). Thus, this method was selected for the analysis of copper ions in tap, bottled water and traditional Chinese medicine samples under optimized experimental conditions.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Huimin Zhao,Aiping Yang,Nan Zhang,Shiyang Li,Tianjie Yuan,Ning Ding,Siwang Zhang,Sheng Bao,Chang Wang,Yinan Zhang,Xiachang Wang,Lihong Hu
The discovery of new, safe, and effective pesticides is one of the main means for modern crop protection and parasitic disease control. During the search for new insecticidal secondary metabolites from endophytes in Stemona sessilifolia (a traditional Chinese medicine with a long history as an insecticide), 10 new insecticidal endostemonines A–J (1–10) were identified from an endophytic Streptomyces sp. BS-1. Their structures were determined by comprehensive spectroscopic analysis. Endostemonines A–J represent the first reported naturally occurring pyrrole-2-carboxylic ester derivatives, which consisted of different fatty acid chains at the C-2 of pyrrole ring were produced by traditional Chinese medicine endophytic microbes. All new tested compounds exhibited strong lethal activity against Aphis gossypii (LC50 value range of 3.55–32.00 mg/L after 72 h). This research highlighted the discovery of pesticide natural products from insecticidal medicinal plant endophytes for the first time, paving a new pathway for the development of pest control.
研究领域:
植物学
·
农药开发
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
FUEL PROCESSING TECHNOLOGY
·
2022
·
IF 8.129
Hao Bi,Zhiwei Wang,Hao Zhan,Lijian Leng,Zhiyong Zeng,Xinming Wang,Huacai Liu,Xiuli Yin,Chuangzhi Wu
Chinese herbal residue (CHR) is an association of renewable resource and hazardous waste , and high nitrogen content and low energy density are two primary obstacles to its efficient and clean thermal utilization. In this paper, acid-promoted hydrothermal was employed to probe the feasibility on CHR toward enhanced solid biofuel concerning upgradation and denitrogenation. A more excellent hydrothermal promoter (H 2 SO 4 ) was demonstrated to upgrade and denitrogenate CHR with better energy (1.125) and nitrogen densities (0.586) at optimal conditions (160 °C, 0.5 N H 2 SO 4 ), which could be attributed to the improved removal of oxygen, amino and ash during acid-promoted hydrothermal. Compared to raw feedstock or the conventional hydrochar , the acid-promoted hydrochar had enhanced compositional characteristics with higher heating value (21.00 kJ·mol −1 ), lower nitrogen (1.44 wt%) and ash (11.03 wt%) contents, thus showing more stable combustion flame with a higher comprehensive combustion index of 4.861 × 10 −8 , a higher combustion stability index of 4979.342, lower combustion alkali index of 0.026, and less nitrogen gaseous pollutant emission. Thus, acid-promoted hydrothermal would be a promising way to convert CHR into much cleaner and higher-grade solid biofuel.
研究领域:
能源与环境科学
·
生物质能工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Sensors
·
2025
·
IF 9.1
Ruijie Wu,Ziyihui Wang,Yaoxin Fu,Junfeng Jiang,Yu-Cheng Chen,Tiegen Liu
Hydrogels have emerged as promising sensors for detecting heavy metal ions in fluids and have been extensively developed. However, monitoring of multiple target analytes in Chinese herbs remains challenging due to subtle chemical signals and the complex composition of the extracted solutions. To address these challenges, we developed a hydrogel optofluidic sensor to amplify analyte signals through strong light–matter interactions within the hydrogel. This sensing platform integrates a hydrogel film encapsulated in a whispering-gallery-mode (WGM) microcavity for the detection of heavy metal ions, such as Pb2+ and Hg2+. The 3D cross-linked hydrophilic polymer network facilitates ion penetration from analyte solutions, inducing distinct WGM resonance shifts. The red shift in the spectral wavelength serves as a parameter to quantify the content of heavy metal ions. By modification of the hydrogel with aptamers, the optofluidic sensors achieve high sensitivity and selectivity. Finally, the platform’s performance was demonstrated using Chinese herbs with varying Pb2+ concentrations, highlighting its practical applicability in real-world scenarios. The proposed hydrogel microcavity exhibit a promising method for development of functional hydrogel sensors and healthcare applications.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 13
·16655-16662
·
IF 4.223
Xiao Jinjing,Ma Jinjuan,Wang Fan,Xu Xing,Liao Min,Shi Yanhong,Cao Haiqun
Numerous natural preparations in traditional Chinese medicine are prepared as decoctions. Processing factors (PFs) comparing the levels of pesticide residues in decoctions to those in the corresponding unprocessed products should be considered in exposure assessments. Thus, this study determined the residue levels of six pesticides (chlorpyrifos, phoxim, imidacloprid, thiamethoxam, fenpropathrin, and emamectin benzoate), as well as 3,5,6-trichloropyridinol, the primary metabolite of chlorpyrifos, and clothianidin, the main metabolite of thiamethoxam in Baishao, Paeoniae radix lactiflora (Fam. Ranunculaceae). The results showed that significant time-response effects were present for the release of pesticides from P. radix. The PFs calculated were < 1, indicating a significant reduction in pesticide residues after TCM processing. The water solubility and partition coefficient values of the pesticides may have played a basic role in the dissipation of the residues during the TCM decocting process. A risk assessment based on the hazard quotient with PFs revealed that exposure to pesticide residues in P. radix was far below the levels that might pose a health risk. In conclusion, the results presented here are of theoretical and practical value for the safety evaluation of TCMs.
研究领域:
药理学
·
毒理学
·
中医药学
✓ 已验证
DOI
高影响因子
JOURNAL OF CELLULAR PHYSIOLOGY
·
2018
·Vol. 234
·期 5
·7161-7173
·
IF 3.923
Xuzhao Wang,Yafei Chen,Junwei Li,Shuai Guo,Xiaoe Lin,Hailin Zhang,Yong Zhan,Hailong An
Mortality-to-incidence ratios in patients with cancer are extremely high, positioning cancer as a major cause of death worldwide. Ether-à-go-go-1 (Eag1) is an ion channel that plays important roles in tumour proliferation, malignant transformation, invasion, metastasis, recurrence, and prognosis. Therefore, identifying potent and specific Eag1 channel inhibitors is crucial. In this study, we identified the first natural inhibitor of Eag1, the traditional Chinese medicine agent tetrandrine, and explored the underlying mechanism. Tetrandrine directly interacted with Eag1 and inhibited the currents in a concentration-dependent manner (IC 50 of 69.97 ± 5.2 μM), and the amino acids Ile 550 , Thr 552 , and Gln 557 in the Eag1 C-linker domain were critical for tetrandrine’s inhibitory effect. Moreover, tetrandrine reduced the proliferation of HeLa cells and Chinese hamster ovary (CHO) cells stably expressing Eag1 in a concentration-dependent manner. Finally, tetrandrine (30 mg/kg/day) inhibited tumor growth in mice, demonstrating a 64.21% inhibitory rate of HeLa cell-transplanted tumors. These results suggest that tetrandrine is a potent and selective Eag1 channel inhibitor, and could act as a leading compound in the development of therapies for Eag1 ion channel dysfunction-induced diseases.
研究领域:
药理学
·
肿瘤学
·
离子通道研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2022
·Vol. 45
·期 18
·3404-3411
·
IF 3.614
Yudan Wang,Cheng Zhang,Lei Zhang,Zhen OuYang,Ming Zhao,Jiaoyang Luo,Meihua Yang
In this study, a sensitive and accurate immunoaffinity columns coupled with high-performance liquid chromatography method was established to monitor the presence of aflatoxins—aflatoxin B 1 , aflatoxin B 2 , aflatoxin G 1 , and aflatoxin G 2 —in different medicinal herbs. The proposed method was found to be suitable for the detection of aflatoxins in eight kinds of herbs and their corresponding granules. Two batches of Arecae semen were positive for aflatoxins, with high residue levels of different aflatoxins. To better understand the presence and transfer of aflatoxins during the formulation of dispensing granules from the herbs, the aflatoxins-free herbs were artificially inoculated with Aspergillus flavus to explore aflatoxins production. Both aflatoxin B 1 and aflatoxin B 2 were detected in all inoculated samples, while aflatoxin G 2 was only detected in Astragali radix samples. Additionally, the presence of aflatoxin B 1 was extremely high compared to other aflatoxins. More specifically, the transfer rate of the aflatoxin B 1 and the total aflatoxins from original herbs to granules were both approximately 40%. These findings indicated that the preparation of herbs into dispensing granules reduced the content of aflatoxins. The high-level presence of aflatoxins in inoculated herbs indicated that attention is needed to the safety of A. flavus -contaminated herbs.
研究领域:
药学
·
食品安全
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2012
·Vol. 4
·期 4
·1012-1018
·
IF 1.547
Xiaojie Liu,Yuzhi Wang,Jinhuan Kong,Chan Nie,Xiao Lin
Eight types of ionic liquid (IL) were synthesized in this paper, and ionic liquids-based microwave-assisted extraction (ILs-MAE) technique was used to extract quercetin from the Chinese herbal medicine Toona sinensis. Several significant operating parameters were optimized by a series of single factor experiments and orthogonal design L9 (34) experiments in this work. Compared with liquid-based maceration extraction (ILs-ME), liquid-based heat extraction (ILs-HE) and liquids-based ultrasound-assisted extraction (ILs-UAE), the optimized approach of ILs-MAE technique cost the shortest time but afforded the highest extraction amount. Moreover, reversed phase high performance liquid chromatography (RP-HPLC) with ultraviolet detection (UV-DAD) was employed for the analysis. The results show that the amount of quercetin extracted by [Bmim]Br was 182.78 mg g−1 with RSD 1.02%. The developed approach is linear between concentrations from 15.20 mg L−1 to 213.2 mg L−1 of quercetin and the regression coefficient (R) was 0.9992. The recoveries of quercetin extracted by these four methods were 95.07%–105.8% with RSDs lower than 3%. Furthermore, the change of the microstructure and chemical structure of the sample before and after different extraction procedures were researched, respectively. Because of the remarkable dissolving capacity of ILs aqueous solution and the obvious destruction on sample surface microstructures during ILs-MAE process, ILs-MAE technique got the highest extracted amount. With the help of LC-MS, we can further confirm that Toona sinensis do contain the active ingredient of quercetin and ILs had no interference of quercetin.
研究领域:
药学
·
分析化学
✓ 已验证
DOI
高影响因子
Desalination and Water Treatment
·
2024
·
IF 1
Shuang Wu,Yansong Zhang,Shengnan Kong,Haiyang Yan,Chuanrun Li,Huchuan Wang
s In this paper, a hybrid system of ultrafiltration and membrane distillation was used to study the resourceful reuse of active compounds and water resources in traditional Chinese medicine wastewater. Pre-treatment of turbid wastewater by ultrafiltration (UF) was found to maximise the retention of macromolecular organic matter in the wastewater using a 10 kDa ultrafiltration membrane at a trans-membrane pressure of 0.15 MPa. The UF filtrate was subjected to vacuum membrane distillation (VMD) at 60 °C, 95 kPa, 36 L·h −1 , and pH = 3, which ultimately recovered about 85 % of chlorogenic acid as well as about 74 % of baicalin. The UF-MD system can effectively separate the large organic molecules, small molecules of active compounds and water in the turbid wastewater of traditional Chinese medicine, so as to realise the recovery and reuse of valuable resources in the turbid wastewater of traditional Chinese medicine.
研究领域:
环境科学
·
中药学
·
水资源管理
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）