ACS Catalysis
·
2021
·
IF 13.084
Yu Gu,Shibin Wang,Huaijie Shi,Jun Yang,Suiqin Li,Haiyang Zheng,Wenbin Jiang,Jia Liu,Xing Zhong,Jianguo Wang
Environment-friendly electrochemical ozone production (EOP) is a promising route for in situ sterilization and discoloration applications. Herein, a Pt-SAs/BNC catalyst with Pt single atoms embedded into B/N codoped nanotubes is rationally designed and prepared. The process provides a local environment rich in oxygen intermediates and hence improves the EOP performance in neutral media by influencing the EOP thermodynamics and kinetics. The Pt-SAs/BNC catalyst exhibits excellent EOP performance with a Faraday efficiency of 21% at the current density of 50 mA cm–2, which is almost twice as much as that of commercial β-PbO2. Density functional theory calculations further reveal the synergistic effect between the B sites and the Pt single atom of the EOP mechanism. In addition, the Pt-SAs/BNC catalyst presented a significant performance in electrodegradation of various pesticides and antibiotics, indicating the potential environmental application capabilities of Pt-SAs/BNC.
研究领域:
材料科学
·
环境科学
·
催化化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Sensors
·
2024
·
IF 8.2
Fang-Kun Yang,Jie Cao,Ting Zhang,Hao-Xiang Jiang,Han-Bin Cui,Kai Wang
Cardiovascular disease (CVD) is a chronic disease characterized by the accumulation of lipids and fibrous tissue within the arterial walls, potentially leading to vascular obstruction and an increased risk of heart disease and stroke. Hydroxyl radicals play a significant role in the formation and progression of CVD as they can instigate lipid peroxidation, resulting in cellular damage and inflammatory responses. However, precisely detecting hydroxyl radicals in CVD lesions presents significant challenges due to their high reactivity and short lifespan. Herein, we present the development and application of a novel activatable optical probe, Cy-OH-LP, designed to detect hydroxyl radicals in lipid-rich environments specifically. Built on the Cy7 molecular skeleton, Cy-OH-LP exhibits near-infrared absorption and fluorescence characteristics, and its specific response to hydroxyl radicals enables a turn-on signal in both photoacoustic and fluorescence spectra. The probe demonstrated excellent selectivity and stability in various tests. Furthermore, Cy-OH-LP was successfully applied in an in vivo model to detect hydroxyl radicals in mouse models, providing a potential tool for diagnosing and monitoring AS. The biosafety of Cy-OH-LP was also verified, showing low cytotoxicity and no significant organ damage in mice. The findings suggest that Cy-OH-LP is a promising tool for the specific detection of hydroxyl radicals in lipid-rich environments, providing new possibilities for research and clinical applications in the field of oxidative stress-related diseases.
研究领域:
心血管疾病
·
光声成像
·
分子探针
·
生物医学工程
·
氧化应激研究
✓ 已验证
DOI
PubMed
高影响因子
NATURE BIOTECHNOLOGY
·
2022
·Vol. 40
·期 11
·1601-1609
·
IF 68.164
Chen Ji,Chen Mengyin,Zhu Ting F.
Mirror-image aptamers made from chirally inverted nucleic acids are nuclease-resistant and exceptionally biostable, opening up opportunities for unique applications. However, the directed evolution and selection of mirror-image aptamers directly from large randomized l -DNA libraries has, to our knowledge, not been demonstrated previously. Here, we developed a ‘mirror-image selection’ scheme for the directed evolution and selection of biostable l -DNA aptamers with a mirror-image DNA polymerase. We performed iterative rounds of enrichment and mirror-image polymerase chain reaction (PCR) amplification of l -DNA sequences that bind native human thrombin, in conjunction with denaturing gradient gel electrophoresis (DGGE) to isolate individual aptamers and l -DNA sequencing-by-synthesis to determine their sequences. Based on the selected l -DNA aptamers, we designed biostable thrombin sensors and inhibitors, which remained functional in physiologically relevant nuclease-rich environments, even in the presence of human serum that rapidly degraded d -DNA aptamers. Mirror-image selection of biostable l -DNA aptamers directly from large randomized l -DNA libraries greatly expands the range of biomolecules that can be targeted, broadening their applications as biostable sensors, therapeutics and basic research tools.
研究领域:
分子生物学
·
生物技术
·
纳米技术
·
治疗学
·
传感器
✓ 已验证
DOI
PubMed
高影响因子
ACS Omega
·
2023
·
IF 4.1
Weilin Liu,Jiansan Li
Corrosion resistance coating applied on Q235 carbon steel in a chloride-rich environment was explored in our research. The coating as a barrier inhibits the penetration of the corrosion medium and provides active corrosion protection for Q235 carbon steel. Halloysite nanotubes (HNTs) were loaded with sodium lignosulfonate (SLS) under vacuum conditions. 4.53% of loading efficiency was validated by thermogravimetric analysis (TGA). The deposition of polyelectrolyte layers including poly(dimethyl diallyl ammonium chloride) (PDDA) and poly(styrenesulfonate) (PSS) not only resulted in controlling the release rate of SLS but also enabled the HNTs to possess pH-responsive release property. The modified HNTs were defined as “PSS/PDDA/SLS/HNTs”, which were characterized by SEM, TEM, FTIR, and zeta potential analyses. TGA elucidates that PSS/PDDA/SLS/HNTs exhibit superior thermal stability. The results of UV–vis spectroscopic analysis confirm that HNTs exhibit a higher release amount in an alkaline medium than in neutral and acidic conditions. Afterward, PSS/PDDA/SLS/HNTs were mixed with the epoxy coating, which was applied on Q235 carbon steel immersed in 3.5 wt % NaCl solution. Electrochemical measurements illustrate the excellent corrosion resistance of the epoxy coating with the addition of PSS/PDDA/SLS/HNTs. Also, water contact angle analysis demonstrates the modification of the epoxy coating with decent hydrophobicity.
研究领域:
材料科学
·
腐蚀工程
·
纳米技术
·
涂层技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BRAIN RESEARCH BULLETIN
·
2023
·
IF 3.8
Xiao Ji,Zhenwu Zhao
Background Silencing of miR-92a-3p may be beneficial in relieving depression of chronically stressed rats. The level of kruppel-like factor 2 (KLF2) was increased in the striatum of depressed rats after ketamine treatment. Enriched environment (EE) ameliorated depression-like behaviors in rats. However, the specific mechanism of EE treatment on depression induced by chronic unpredictable mild stress (CUMS) remains unclear. Methods After CUMS-induced male Sprague Dawley rats were treated under EE or/and Adeno-Associated Virus (AAV)-miR-92a-3p, depression-like behaviors, cognitive ability, dendritic spine density, as well as levels of miR-92a-3p and KLF2 were detected by the behavioral tests, morris water maze test, Golgi staining, and quantitative real-time polymerase chain reaction (qRT-PCR) as needed. The body weight of rats was also measured. Next, primary hippocampal neurons were cultivated. The targeting relationship between miR-92a-3p and KLF2 was analyzed by TargetScan v7.2 and dual-luciferase reporter assay. After hippocampal neurons were transfected with miR-92a-3p mimic or/and overexpressed KLF2 vector, the cell viability, and apoptosis, together with the levels of KLF2, brain-derived neurotrophic factor (BDNF), phosphorylated (p)-tropomysin related kinase B (p-TrkB) and TrkB were determined by MTT assay, flow cytometry, qRT-PCR, and western blot as needed. Results EE ameliorated CUMS-induced depression-like behaviors and cognitive ability, and elevated the neuronal dendritic spine density and KLF2 level, but reduced miR-92a-3p level in hippocampal tissues, while the above effects were reversed by AAV-miR-92a-3p. MiR-92a-3p mimic restrained cell viability, along with p-TrkB/ TrkB and BDNF levels, but promoted apoptosis in hippocampal neurons, which were reversed by overexpressed KLF2. Conclusion EE ameliorates CUMS-induced depression-like symptoms in rats via regulating the miR-92a-3p/KLF2 pathway.
研究领域:
神经科学
·
分子生物学
·
行为科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）