BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
·
2022
·Vol. 130
·期 5
·592-605
·
IF 3.688
Zhiping Jin,Qingfeng He,Xiao Zhu,Mingshe Zhu,Yike Wang,Xin-an Wu,Qianzhou Lv,Xiaoqiang Xiang
Anlotinib is a small molecule of novel tyrosine kinase inhibitor initially approved to treat non-small cell lung cancer in China. Drug–drug interaction (DDI) is an extrinsic factor important for the appropriate use of anlotinib in clinical practice. In vitro experiments demonstrated that anlotinib is a substrate of cytochrome P450 (CYP) enzymes and moderate inhibitor of several common ones; however, no clinical DDI studies have been performed to investigate inhibitory effects of anlotinib on these CYP enzymes. Thus, its drug label recommends avoiding co-administration with substrates of these enzymes, which have narrow therapeutic windows. In this study, we performed a CYP450 inhibition study, followed by gathering in vitro and clinical pharmacokinetic data to build the first physiologically based pharmacokinetic (PBPK) model of anlotinib. The verified model was subsequently used to predict the DDI mediated by anlotinib. As a result, the marginal plasma exposure changes of typical CYP3A and CYP2C9 substrates were less than the bioequivalence threshold, indicating that anlotinib has a very low potential of causing clinically meaningful DDI through the inhibition of several major CYP enzymes. According to the FDA's latest guideline on DDI, the established model with the simulation results may support the revision of anlotinib labelling without further clinical studies, lifting unnecessary restrictions on anlotinib regimens.
研究领域:
药理学
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临床药学
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Qi Zheng,Fan Wang,Jiayu Sun,Mohammed F. Hamza,Qiang Wu,Yehuizi Wu,Ningchao Zheng,Shunyan Ning,Tianjiao Jiang,Deqian Zeng,Hiroshi Watabe,Hao Wu,Yan Wu,Yuezhou Wei,Xiangbiao Yin
As the primary fission product in spent nuclear fuel being generally filed into the high-level liquid waste, the 90 Sr recovered from the reprocessed nuclear fuel could be a significant source for producing 90 Y nuclide (the decay daughter). It presents as one favorable therapeutic radionuclide candidate for radiolabeling of pharmaceuticals in widespread clinical application. Under this situation, a novel silica-based adsorbent (HDEHP/SiO 2 -P) was developed for selective separation of Y 3+ from concentrated Sr 2+ in aqueous solution. Batch adsorption process was investigated under different parameters of acid type and strength, contact time, temperature, ionic concentration and molar ratio etc. It was confirmed that, the HDEHP/SiO 2 -P had highly selective properties toward Y 3+ over Sr 2+ in different acidic medium (i.e., hydrochloric, nitric and acetic acid), with maximum adsorption capacity 34.05 mg Y 3+ /g. Sorption isotherms and uptake kinetics, were better fitting for Langmuir monomolecular layer adsorption mode and the pseudo-second order (PSORE) kinetic model respectively. The sorbent shows high SF Y/Sr of 1.93 × 10 3 even at a Sr-Y molar ratio of 2 × 10 3 . Moreover, dynamic separation of trace amount of Y 3+ from the concentrated Sr 2+ aqueous solution was successfully validated by the column test, in which 100 % Y 3+ was recovered. The produced eluate is characterized by a sharp elution curve without Sr trailing under high acidic conditions of 6 M HCl. All these results would promisingly promote the application of the developed material and chromatographic approach to facilely obtain the adequate quantities of 90 Y with required radiochemical purity for its widespread clinical use.
研究领域:
核化学与放射化学
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材料科学
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医学
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产品关联
DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 44
·27541-27546
·
IF 3.361
Qingquan Zhang,Jiajia Li,Yuting Su,Xiaoyan Pan,Hongwei Gai
The relatively low sensitivity is the limitation of ELISA in early screening of diseases. Various signal enhancing methods are developed to increase detection sensitivity, but most of them require professional operation and are not compatible with commercial reagent kits or automatic analysis instruments, limiting their application in clinical testing. Here, we present a contactless and user-friendly ball-lens assisted fluorescence enhancing (BFE) method to improve immunoassay sensitivity. The BFE effect is observed in experiment and demonstrated by the simulation. Based on the BFE effect, the detection sensitivity of the immunoassay is improved about 3.6-fold and the limit of detection is lowered by more than 3-fold using commercial reagents and standard ELISA processes. In addition, the BFE effect causes no damage to the linear dependence, specificity and accuracy of clinical plasma measurement. The established method shows a high compatibility with automatic microfluidic immunoassay systems and other signal enhancing techniques, displaying great potential in multi-technique coupling assays and clinical applications.
研究领域:
生物医学工程
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分析化学
·
临床检验
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DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2024
·
IF 3.1
Jiexia Li,Zhuqiao Fan,Hanqi Chen,Ernestina Maria Da Costa,Xiaomian Zhou,Nan Yu
Adiponectin (ADPN), which serum/plasma adiponectin levels are closely associated with insulin resistance and type 2 diabetes , and lower adiponectin levels predict an increased risk of diabetes, is a strong indicator of diabetes risk in people at high risk of diabetes in different races. Using the unique principle and performance advantages of chemiluminescence immunoassay (CLIA), an ADPN-CLIA method with high sensitivity, high specificity and wide detection range was established based on the principle of two-steps method of sandwich-type, with the magnetic particles (MPs) as the solid phase carrier and acridinium ester (AE) as the chemiluminescence reaction system. The selection of the main raw materials required, the preparation conditions of MPs-coated antibodies, the methods of AE-labeled antibodies, sample requirements and reaction modes were optimized and evaluated. AE labeling experiment was successfully performed with the labeling efficiency of 8.366 and the antibody utilization rate of 96.8%. The chemiluminescent immunoassay for ADPN had a good linear relationship from 0 ng/mL to 250 ng/mL (R 2 =0.9993), with the detection limit of 0.05 ng/mL. The coefficient of variation (CV) of intra-assay and inter-assay precision were both less than 5% respectively. The recovery rates for accuracy were from 91.26% to 107.46%. The comparison experiment of 80 clinical serum samples between the developed ADPN-CLIA with the immunoturbidimetry showed that the correlation coefficient was 0.956, and the Bland-Altman analysis showed that the limits of agreement were − 0.364 and 0.433.
研究领域:
医学检验
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生物化学
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产品关联
DOI
PubMed
高影响因子
DIAMOND AND RELATED MATERIALS
·
2024
·
IF 4.3
Chuanqin Zhou,Yingyu Sima,Yiyong Wu,Yanping Wei,Du Tuo,Shuting Shi,Aiting Chen,Nana Tang,Quanguo He,Wu Wang
Clozapine is vital for managing schizophrenia and other psychiatric disorders. As a potent medication with risks of severe side effects, accurate detection ensures effective treatment monitoring, patient safety, and optimization of therapeutic outcomes. Herein, niobium carbide two-dimensional transition metal carbides (Nb 2 CT x MXene) was prepared by etching niobium aluminum carbide (Nb 2 AlC), which was next partially oxidized by a hydrothermal method to niobium oxide (Nb 2 O 5 )/Nb 2 CT x and ultrasonically bound to carboxylated multi-walled carbon nanotubes (MWCNTs-COOH). It was modified onto a glassy carbon electrode for the detection of the antipsychotic drug clozapine ingested by humans. The morphology and electrochemical properties of MXene-based nanocomposite electrode were verified by scanning electron microscopy, X-ray diffractometry, Raman, cyclic voltammetry and electrochemical impedance characterization methods. The results showed that electrode responded well to clozapine solutions, with good linearity ( R 2 = 0.9979 and 0.9995) of the peak currents with clozapine concentrations (0.01–1.0 μM and 1.0–10.0 μM) and that this sensor had good immunity, repeatability, reproducibility and stability. The limit of detection was 8.38 nM and the recovery was 92.5 % ~ 106.5 % in real serum and urine samples. The MXene-based sensors offer excellent analytical performance and hold promise for cost-effective generalisation to the detection of nanomolar clozapine in a variety of clinical applications.
研究领域:
分析化学
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医学检测
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材料科学
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产品关联
DOI
高影响因子
PHARMACOLOGICAL RESEARCH
·
2024
·
IF 9.1
Bo Qin,Shi-jie Fu,Xiong-fei Xu,Jiu-jie Yang,Yuping Wang,Lin-na Wang,Bai-xiong Huang,Jing Zhong,Wan-yu Wu,Heng-ao Lu,Betty Yuen Kwan Law,Nick Wang,Io Nam Wong,Vincent Kam Wai Wong
In future regenerative medicine, far-infrared radiation (FIR) may be an essential component of optical therapy. Many studies have confirmed or validated the efficacy and safety of FIR in various diseases, benefiting from new insights into FIR mechanisms and the excellent performance of many applications. However, the lack of consensus on the biological effects and therapeutic parameters of FIR limits its practical applications in the clinic. In this review, the definition, characteristics, and underlying principles of the FIR are systematically illustrated. We outline the therapeutic parameters of FIR, including the wavelength range, power density, irradiation time, and distance. In addition, the biological effects, potential molecular mechanisms, and preclinical and clinical applications of FIR are discussed. Furthermore, the future development and applications of FIR are described in this review. By applying optimal therapeutic parameters, FIR can influence various cells, animal models, and patients, eliciting diverse underlying mechanisms and offering therapeutic potential for many diseases. FIR could represent a superior alternative with broad prospects for application in future regenerative medicine.
研究领域:
医学
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生物学
·
光学疗法
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DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Dong Xiang,Tinghui Zhao,Jue Wang,Yang Cao,Qiuxia Yu,Lu Liu,Hengyi Yu,Xiping Li,Ninghong Li,Yang Yi,Xuepeng Gong
A sensitive and robust LC-MS/MS method has been developed and validated for olverembatinib quantification in human plasma and cerebrospinal fluid (CSF). The method involved liquid-liquid extraction with methyl tertiary butyl ether for plasma pretreatment and precipitation enrichment with methanol for CSF pretreatment. Separation was achieved on the C18 column with gradient elutions of 10 mM ammonium formate in water and methanol-acetonitrile (50:50,v/v). Analyte detection was conducted by electrospray ionization (ESI) in a positive ion mode using multiple reaction monitoring (MRM). The m/z transitions were 533.4→433.2 for olverembatinib and m/z 502.4→394.2 for the internal standard (IS, Imatinib-d8). Calibration curves ranged from 0.500 to 50.0 ng/mL for plasma and from 0.0100 to 1.00 ng/mL for CSF. The intra- and inter-day precision and accuracy were < 15% for both plasma and CSF with four different quality control concentrations. The relative matrix effect was < 10% in plasma and artificial CSF. This method was successfully utilized for the measurement of olverembatinib concentrations in plasma and CSF from chronic myeloid leukemia patients.
研究领域:
药理学
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分析化学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
Science Advances
·
2021
·Vol. 7
·期 23
·
IF 14.143
Zhaoxiang Yang,Yonglin He,Shenglong Liao,Yingchao Ma,Xinglei Tao,Yapei Wang
Hydrogel coatings pave an avenue for improving the lubricity, biocompatibility, and flexibility of solid surfaces. From the viewpoint of practical applications, this work establishes a scalable method to firmly adhere hydrogel layers to diverse solid surfaces. The strategy, termed as renatured hydrogel painting (RHP), refers to adhering dehydrated xerogel to a surface with appropriate glues, followed by the formation of a hydrogel layer after rehydration of the xerogel. With the benefits of simplicity and generality, this strategy can be readily applied to different hydrogel systems, no matter what the substrate is. Hydrogel adhesion is demonstrated by its tolerance against mechanical impact with hydrodynamic shearing at 14 m/s. This method affords powerful supplements to renew the surface chemistry and physical properties of solid substrates. In addition, we show that the RHP technique can be applied to living tissue, with potential for clinical applications such as the protection of bone tissue.
研究领域:
材料科学
·
生物医学工程
·
表面化学
✓ 已验证
DOI
高影响因子
ACS Sensors
·
2023
·
IF 8.9
Chenglong Lin,Zhenzhen Liu,Fanghao Fang,Shuai Zhao,Yanyan Li,Meimei Xu,Yusi Peng,Hongyou Chen,Fang Yuan,Wanju Zhang,Xi Zhang,Zheng Teng,Rui Xiao,Yong Yang
The coronavirus disease 2019 (COVID-19) pandemic highlighted the need for rapid and accurate viral detection at the point-of-care testing (POCT). Compared with nucleic acid detection, lateral flow immunoassay (LFIA) is a rapid and flexible method for POCT detection. However, the sensitivity of LFIA limits its use for early identification of patients with COVID-19. Here, an innovative surface-enhanced Raman scattering (SERS)-LFIA platform based on two-dimensional black phosphorus decorated with Ag nanoparticles as important antigen-capturing and Raman-signal-amplification unit was developed for detection of SARS-CoV-2 variants within 5–20 min. The novel SERS-LFIA platform realized a limit of detection of 0.5 pg/mL and 100 copies/mL for N protein and SARS-CoV-2, demonstrating 1000 times more sensitivity than the commercial LFIA strips. It could reliably detect seven different SARS-CoV-2 variants with cycle threshold (Ct) < 38, with sensitivity and specificity of 97 and 100%, respectively, exhibiting the same sensitivity with q-PCR. Furthermore, the detection results for 48 SARS-CoV-2-positive nasopharyngeal swabs (Ct = 19.8–38.95) and 96 negative nasopharyngeal swabs proved the reliability of the strips in clinical application. The method also had good specificity in double-blind experiments involving several other coronaviruses, respiratory viruses, and respiratory medications. The results showed that the innovative SERS-LFIA platform is expected to be the next-generation antigen detection technology. The inexpensive amplification-free assay combines the advantages of rapid low-cost POCT and highly sensitive nucleic acid detection, and it is suitable for rapid detection of SARS-CoV-2 variants and other pathogens. Thus, it could replace existing antigens and nucleic acids to some extent.
研究领域:
医学诊断
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病毒学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2022
·
IF 5.141
Shengnan Fan,Xiaoqing Li,Fanghui Ma,Minghui Yang,Juan Su,Xiang Chen
Lactate dehydrogenase (LDH) plays an important role in the process of glycolysis as well as gluconeogenesis, and is related to many diseases, so it is of great significance to accurately detect LDH activity in serum. The fluorescence of sulfur quantum dots (SQDs) synthesized by the microwave method can be specifically enhanced by nicotinamide adenine dinucleotide (NAD + ). Based on this characteristic, we reported a sensitive fluorescence assay for LDH detection. In the presence of reductive coenzyme I (NADH), LDH catalyzes the reduction of pyruvate to lactic acid, and transforms NADH to NAD + , which specifically enhances the fluorescence intensity of SQDs. Based on the fluorescence intensity increase, the assay achieved detection of LDH in the range from 500 to 40,000 U L −1 , with limit of detection of 262 U L −1 . The assay was successfully applied for the detection of LDH activity in serum, indicating potential clinical applications.
研究领域:
生物化学
·
分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
ELECTROANALYSIS
·
2023
·Vol. 35
·期 10
·e202300158
·
IF 3
Ke Jiang Li,Juan Li,Fan Shi,Li Peng Gan,Wei Sun,Zhi Song Lu,Zhuo Zou,Chang Ming Li
Dopamine (DA) plays an essential role in the central nervous, renal, hormonal and cardiovascular systems. Various modified carbon nanotubes (CNT)-based dopamine sensors have been reported, but inexpensive, highly sensitive plain CNT-based ones are seldom studied. In this work, a facile and inexpensive CNT-based DA sensor is made by rich-defect multi-walled carbon nanotubes (RD-CNT) via an ultrasound method. The defect and elemental states of the RD-CNT are systematically studied by transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM), Raman spectroscopy, X-ray powder diffraction (XRD) and X-ray-photoelectron spectroscopy (XPS). Results show that massive holes and cracks exist in RD-CNT. The level of defects increases from the additional exposed edges. The electrochemical characterizations indicate that the electrochemical sensor has the highest sensitivity of 438.4 μA/(μM ⋅ cm 2 ) among all carbon materials-based DA sensors while well meeting the clinically required detection range and selectivity. The DA sensor was further used to detect live healthy human serum and live PC12 cells with satisfactory results, thus holding great promise for an inexpensive but sensitive DA sensor in practical applications of clinical diagnosis and biological research.
研究领域:
生物医学工程
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材料科学
·
神经科学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 17
·1995-2004
·
IF 2.896
Hongliang Li,Chao Guo,Qianchun Zhang,Linchun Bao,Qingfeng Zheng,Zhenpeng Guo,Yi Chen
How to further improve the throughput of capillary electrophoresis (CE) is a fascinating question. Herein an idea to substantially increase the throughput of CE has been proposed together with theory and experimental demonstration. The key is to introduce samples for CE, one after another, by a short suspension of voltage application, which was hence termed separation-interrupted sequential injections (Sisi). The idea was demonstrated to be feasible on a laboratory-built CE instrument coupled with tandem C4D (contactless capacitively-coupled conductivity) detectors. At least 50 injections of a testing sample (mixture of NH4+, K+, Ca2+, Na+ and Mg2+) were successfully separated in only a single run. The separation took 145 min in total, equivalent to 2.9 min per analysis which is only 21% of that of normal CE. Quantification of the separated ions was performed, with a limit of detection of 1.1–2.6 μM, a limit of quantification of 3.2–8.9 μM, and a linear range up to 1000 μM (R2 > 0.99). The recovery was between 88% and 112% measured by spiking standards into samples at low, middle and high levels. The real applicability of Sisi-CE was evaluated by direct injection and analysis of 45 mineral water samples also in a single run. Its clinical application potential was demonstrated by high throughput assay of the calcium and zinc gluconate oral solution formula, and the blood potassium of hyperkalemia and hypokalemia from patients with renal failure disease. This method can be extended to other applications such as omics studies through the use of more suitable detectors. The theory proposed may also be applicable to other high throughput methods.
研究领域:
分析化学
·
医学检验
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产品关联
DOI
PubMed
高影响因子
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