RSC Advances
·
2020
·Vol. 10
·期 61
·36971-36979
·
IF 3.119
Hua Tian,Yongcheng Dai,Wenzhe Fu,Haifang Liu,Mengting Li,Meiyuan Lv,Xueqiong Yin
In this work, a multifunctional ratiometric fluorescence (FL) nanohybrid (CSCDs@DC) was synthesized from chitosan based carbon dots (CSCDs) and dansyl chloride (DC) at room temperature. The CSCDs@DC revealed strong FL intensity, great stability and excellent anti-photobleaching properties. Herein, CSCDs@DC was responsive to pH value in the range of 1.5–4.0 and exhibited color-switchable FL properties between acidic and alkaline environments. In addition, CSCDs@DC showed good selectivity and sensitivity towards Fe3+ ions. A good linear relationship for the Fe3+ ion detection was obtained in the range from 0 μM to 100 μM, with a detection limit of 1.23 μM. What's more, CSCDs@DC can be used as a fluorescent ink. It expressed superior optical properties after 3 months of storage or continuous exposure to UV light for 24 h. This study suggested that CSCDs@DC had potential in the detection of pH and metal ions, as well as showing promising application in the anti-counterfeiting field.
研究领域:
材料科学
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分析化学
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生物医学工程
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2021
·Vol. 44
·期 16
·3052-3060
·
IF 3.645
Cuicui Guo,Xueqin Zhao,Jing Jin,Longxing Wang,Dongqin Tan,Jiping Chen,Yuwen Ni
Human exposure to bisphenols has rarely been reported. The most important challenges in this regard are the sensitivity and accuracy of the analytical methods employed. Dansyl chloride derivatization prior to high-performance liquid chromatography–tandem mass spectrometry has been prevalently employed to improve sensitivity. However, the dose effect of the derivatization reagent on the reaction products is not well understood, especially for reactants with two or more active groups. This study investigated the mass ratio of dansyl chloride to bisphenols and found the mass ratio played a vital role in changing the composition of derivatives; further, the optimal ratio for obtaining di-substituted derivatives was confirmed. Under optimal conditions, solid-phase extraction followed by dansyl chloride derivatization coupled with high-performance liquid chromatography–tandem mass spectrometry was used to detect eight bisphenols in human serum samples. The method detection limits of the eight bisphenols were 0.025‒0.28 ng/mL, and the recoveries were 72.9‒121.7% by spiking bisphenols (2, 5, and 20 ng/mL) into bovine serum. The detection frequencies of bisphenol A and bisphenol F in 73 serum samples obtained from children from Guangzhou were 41.1% and 71.2%, respectively, while the detection frequencies of other bisphenols were below 20%. The concentrations of bisphenol A and bisphenol F were < 0.28‒8.0 ng/mL and < 0.028‒7.6 ng/mL, respectively.
研究领域:
分析化学
·
环境科学
·
毒理学
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DOI
PubMed
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
·
2021
·Vol. 247
·期 11
·2729-2738
·
IF 2.998
Chang Rui,Ma Chong,Yu Chao,Zhang Qiang,Li Yanxin,You Jinmao,Zhang Shijuan
Estrogens in milk samples were extracted by a new ionic liquid (IL)-modified covalent organic framework (COF), and then analyzed by stable isotope labeling (SIL) method using dansyl chloride (DNS-Cl) and d 6 -dansyl chloride (d 6 -DNS-Cl) as SIL agents. The IL-modified COF (TpPa-Ct@Mi) was synthesized by chemically bonding methylimidazole on to the surface of normal COF to make full use of the high surface area and big conjugated structure of COF, and the abundant charge of IL, which are of crucial importance for adsorption. The synthesized COF can form π–π interactions and hydrophobic effect with estrogens, while the IL can form electrostatic interactions with the analytes. It was applied to the dispersive solid-phase extraction (DSPE) of estrogens in milk samples. The extracted estrogen samples were heavy labeled by d 6 -DNS-Cl, and then mixed with the light labeled standard. Samples and standards were mixed and analyzed in a single run, and thus the matrix effect was greatly minimized. The optimized extraction conditions were adsorbent amount, 20 mg; desorption solvent and volume of desorption solvent, 2 mL acetonitrile; NaCl concentration, 0 g/L; sample pH 7. The limits of detection were in the range of 9–13 ng/L, and the limits of quantitation were in the range of 29–40 ng/L. Recoveries and matrix effect were in the range of 89.6–95.5% and 96.1–98.9%, respectively, with relative standard deviations of less than 6.2%.
研究领域:
分析化学
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环境科学
·
食品安全
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DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 11
·1404-1411
·
IF 2.896
Wenxue Xu,Yufeng Hu,Minghuo Wu,Enming Miao,Hao Zhou,Xuwang Zhang,Jingjing Zhan
A method consisting of solid-phase isotope dansylation (derivatization with dansyl chloride) and liquid chromatography-high resolution mass spectrometry (LC-HRMS) was developed for the quantitative analysis of phenolic compounds (phenols) in environmental samples. A magnetic-HLB (hydrophilic lipophilic balanced) material was synthesized and applied as an adsorbent in magnetic solid-phase extraction (MSPE) for the enrichment of the analytical targets. Furthermore, with the solid-phase isotope labeling, the desalting and removal of labeling residuals could be simplified over conventional in-solution labeling. In addition to overcoming the matrix effect by isotope dansylation, the sensitivity for the analysis of phenols by LC-HRMS was remarkably improved by over 100-fold. The method was systematically verified, and good accuracy (86.5–104.9%) and precision (<8.6% and <11.4% for intra- and inter-day, respectively) were achieved for the tested 15 phenols. The limits of detection (LODs) of this method were estimated to be 0.2–5 ng L−1 and 5–100 ng kg−1 in estuary water and sediment samples, respectively. With this method, samples collected from the Daliao River estuary (Panjin, China) were analyzed. It was found that all of the targeted phenols were detected at concentrations ranging from unquantifiable to 485 ng L−1 (the total concentration of analytes found in each sample were in the range 822–957 ng L−1) and unquantifiable to 1368 ng kg−1 (the total concentration of analytes found in each sample were in the range 2251–2992 ng kg−1) in water and sediment, respectively.
研究领域:
环境科学
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分析化学
·
水质
·
环境监测
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Chunjian Wu,Li Wang,He Li,Shujuan Yu
The presence of potential carcinogen 4(5)-Methylimidazole (4-MeI) in foods causes much concern, stressing the need of a sensitive determination means. Here, we proposed a high-sensitivity method to determine 4-MeI in biscuits using dansyl chloride derivatization and disperse liquid-liquid micro-extraction (DLLME) followed by a liquid chromatography-ion trap mass spectrometry (LC-IT-MS) analysis. This developed method was subsequently compared to the solid phase extraction combining with liquid chromatography-triple quadrupole mass spectrometry (SPE-LC-QqQ-MS) method. The optimized derivatization conditions were 30 °C and 10 min. Results suggested that the column retention time (RT) was significantly extended, and the MS signal response of 4-MeI-dansyl derivative was also amplified. The developed DLLME-LC-IT-MS method in 4-MeI determination provided satisfactory linearity in a range of 0.5–300 ng/mL (R 2 > 0.9991), inter-day accuracy (87-102%) and precision (%RSD ≤ 13.6%) with the quantification limit of 0.2 ng/mL, which obtained similar results comparing to the conventional SPE-LC-QqQ-MS method.
研究领域:
分析化学
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食品科学
·
质谱分析
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DOI
PubMed
高影响因子
ANNALS OF CLINICAL AND LABORATORY SCIENCE
·
2019
·Vol. 49
·期 2
·204-211
·
IF 0.96
Xue Xue,Hucheng Chen,Qingqing Xia,Ping He,Yuan Lv,Peng Liu,Yan Yang,Chaochao Wu,Qiang Gao,Jun Peng,Yang Yu,Feng Wang,Wei Qu,Zizheng Wang
Background The measurement of plasma catecholamines (CAs) including dopamine (DA), epinephrine (E), and norepinephrine (NE) and theirderivatives including metanephrine (MN), normetanephrine (NMN), vanillylmandelic acid (VMA), and homovanillic acid (HVA) hasbeen used in the diagnosis of pheochromocytoma and paraganglioma (PPGL) and primary hypertension (PH) but are typically detectedindividually when clinical testing. In this study, pre-column derivatization with dansyl chloride (DNS-Cl) combined with anultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to simultaneously quantifyHVA, VMA, MN, NMN, DA, E, and NE in the plasma from patients with PPGL and PH. Methods Plasma samples were extracted by acetonitrile and derivatized with DNS-Cl, followed by reverse phase separation and triplequadruple detection. Quantification of the CAs and their derivatives in 10 PPGL, 10 PH, and 100 healthy subjects was performedby UPLC-MS/MS analysis. Results All the values of detected CAs/derivatives were in the linearity ranges of the fitted curves. The expression levels of theseven CAs in the PPGL and PH patients were significantly higher than the healthy controls, suggesting increased CA productionin the former. There were significant differences in plasma NE, NMN, and VMA levels between the PPGL and PH patients, butthere was no significant difference in plasma E, MN, DA, and HVA. A discriminant analysis showed that 90% of the final caseswere classified correctly based on the detected CAs/derivatives. Conclusions Our results show that the combined detection of the seven CAs/derivatives could be used for the clinical diagnosis of PPGLand PH.
研究领域:
临床化学
·
内分泌学
·
药理学
·
分析化学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2025
·
IF 5.2
Shan Zhao,Qiyan Huang,Yiran Wang,Yujing Wang,Wenyu Wang,BenxuYang,Aiguo Feng,Chao Li,Shufen Wu,Changlu Wang
Pancreatic lipase inhibitors are promising agents for reducing dietary fat absorption and improving obesity-related complications. This study systematically investigated the inhibitory effects of two Monascus yellow pigments, ankaflavin (AK) and monascin (MS), on porcine pancreatic lipase (PPL) using enzyme kinetics, spectroscopy, and molecular docking approaches. Both AK and MS exhibited dose-dependent and reversible non-competitive inhibition, with IC 50 values of 50.2 ± 1.4 μM and 120.6 ± 1.8 μM, respectively. Fluorescence and circular dichroism (CD) analyses revealed that AK and MS binding induced conformational changes in PPL, as evidenced by an increase in α-helix content and altered micro-environment around tyrosine (Tyr) and tryptophan (Trp) residues. Notably, AK elicited more pronounced structural perturbations than MS. UV–visible spectroscopy confirmed the formation of intermolecular charge-transfer (CT) complexes upon the binding of AK and MS to PPL, verifying their direct interaction. Molecular docking and chemical modification identified key residues (Lys, Arg, Glu, and Tyr) that were essential for inhibitor binding. Furthermore, the combination of AK, MS, and Orlistat produced a synergistic inhibitory effect on PPL activity (combination index <0.57). These results establish AK and MS as potent natural PPL inhibitors and suggest that their anti-obesity potential stems from the structural modulation of PPL.
研究领域:
生物化学
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分子生物学
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酶学
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天然产物研究
·
肥胖与代谢紊乱
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Meng Zhang,Yanxiao Jiang,Qiong Wu,Hong Zhang,Kai Yu,Guangfeng Kan,Daqian Song,Jie Jiang
The direct mass spectrometric analysis of complex biological samples is often hindered by matrix interference and the need for extensive sample preparation. In this study, an online electroextraction method based on nano-electrospray mass spectrometry (OEE–nESI–MS) was proposed for the high-sensitivity and rapid analysis of urine samples. Compared with other methods, OEE–nESI–MS requires only 5 μL of sample and 35 μL of extraction solvent to achieve the migration and simultaneous ionization of the target from the sample solution to the extraction solvent in the nano tip, and online extraction and simultaneous analysis can be achieved within 8 min, while maintaining comparable sensitivity to other detection methods. The OEE–nESI–MS showed good linearity (R2 > 0.995) in the range of 1–600 ng mL–1 for the five catecholamines (CAs) in urine, with detection limits as low as 0.39–0.89 ng mL–1 with recoveries of 91.9–104.9% (RSD ≤ 8.9%). Exploration of the OEE–nESI mechanism emphasized the electric field applied as a key factor facilitating online electroextraction. The OEE–nESI–MS was successfully applied to the analysis of five CAs in real urine samples. This advancement provides a very simplified solution for the online rapid, highly sensitive, and quantitative determination of complex samples.
研究领域:
分析化学
·
质谱分析
·
生物分析化学
·
临床诊断
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Jiana Lin,Ying Chen,Gongke Li,Yuling Hu
Monitoring seafood freshness is critical for ensuring nutritional value and food quality, but targeted analysis in complex matrices remains challenging. Herein, a thiol-functionalized calix[6]arene covalent organic framework grafted with AuNPs, denoted as CX6-SCOF-AuNPs, was strategically designed for rapid, sensitive and selective detection of tryptamine in seafood by surface-enhanced Raman spectroscopy (SERS). CX6-SCOF-AuNPs featured extensive pocket-like hydrophobic cavities integrated within an ordered porous structure, enabling specific capture of tryptamine with exceptional enrichment capacity (203.4 mg/g) and rapid adsorption kinetics (within 2 min). The selectivity mechanism stemmed from the formation of a ground-state charge-transfer complex driven by specific “pocket effect” between CX6-SCOF and size-matched tryptamine. CX6-SCOF-AuNPs was successfully applied to enrichment and detection of tryptamine in seafood, achieving high sensitivity (LOD of 3.4 μg/L) and satisfactory recoveries (90.6 % – 107.4 %). This work provides a promising strategy for supramolecular recognition and targeted SERS analysis of trace hazards in complex food matrices.
研究领域:
分析化学
·
食品安全
·
纳米材料
·
超分子化学
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光谱学
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食品质量控制
·
分子识别
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Bairong Fu,Dongqin Tan,Jing Jin,Lei Tong,Longxing Wang,Yihou Peng,Ningbo Geng,Lidong Wu,Huilian Ma,Yuwen Ni,Jiping Chen
The fragmentation behavior of isomeric hydroxylated polybrominated diphenyl ethers (OH-PBDEs) remains poorly elucidated, although LC-MS/MS is essential for their analysis. This study systematically characterizes their fragmentation behavior by LC-ESI-MS2, elucidating relationships between product ions, structural features, and instrument detection limits. Results demonstrated congener-specific variability in the signal intensity of [M–H]−, particularly among isomers. Fragmentation behavior is highly dependent on the hydroxyl location on the diphenyl ether scaffold, as well as the bromine substituent pattern. Three distinct mechanisms are identified: (1) Br abstraction via free radical-mediated rearrangement cleavage in meta-hydroxylated PBDEs, (2) preferential formation of the [Br]− ion via γ-elimination in ortho-hydroxylated PBDEs, and (3) characteristic formation of bromobenzoquinone anions via multistep fragmentation mechanisms in para-hydroxylated PBDEs. Furthermore, the ortho-effect, particularly pronounced in 2′–OH substituted congeners, significantly alters the fragmentation pathways. While the elucidated fragmentation pathways provided structural insights, the inherent weak signals challenged detection. A sensitivity-enhancing precolumn derivatization protocol with dansulfonyl chloride was introduced to solve the problem. The MS2 fragmentation of these derivatives was conveniently dominated by the cleavage of the C–S bond within the dansyl group, yielding abundant diagnostic ions that greatly aided in structural confirmation. As predicted, the derivatization strategy achieved a remarkable up to 15-fold improvement in the sensitivity. This investigation advances the understanding of OH-PBDE fragmentation, establishes an identification framework using specific patterns and diagnostic ions, and demonstrates a practical derivatization approach for enhanced sensitivity, elucidating structure-fragmentation relationships in brominated aromatics and providing methodological guidance for future analysis.
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PubMed
高影响因子
Foods
·
2026
·Vol. 15
·期 13
·2256
·
IF 6
Yifan Ren,Ruixue Cao,Zhunyao Zhu,Xiaopeng Zou,Longqi Gu,Xiangzhong Zhao
The accumulation of histamine in fish products represents a significant food safety issue, particularly in skipjack tuna (Katsuwonus pelamis), due to its elevated histidine content. This study sought to isolate histamine-producing bacteria from skipjack tuna and assess the inhibitory effects of six plant-derived essential oils on bacterial proliferation and histamine synthesis. Seven bacterial isolates were obtained and screened, with histamine concentrations quantified via high-performance liquid chromatography (HPLC) following dansyl chloride derivatization. The isolate exhibiting the highest histamine production (1.2 ± 0.2 mM) was identified asAeromonas hydrophilathrough 16S rDNA sequencing. Essential oils were administered to bacterial cultures prior to histamine quantification, and their minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) were determined in vitro. Among the tested oils, oregano and cinnamon demonstrated the strongest antibacterial activity, with MIC and MBC values below 1 mg/mL. Scanning electron microscopy analysis revealed pronounced structural damage to bacterial cells treated with these oils. At the MBC, histamine production was entirely suppressed; at half the MBC, histamine synthesis was reduced by more than 90%, whereas lower concentrations yielded moderate inhibition ranging from 15% to 22%. These findings suggest that selected essential oils, notably oregano and cinnamon, possess considerable potential as natural preservatives to reduce histamine formation in skipjack tuna. However, further investigation is necessary to confirm their effectiveness under practical storage conditions.
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PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-15
·
IF 15.7
Ma Yanfei,Zhong Yifan,Tang Wenjie,Valencak Teresa G.,Liu Jingliang,Deng Zhaoxi,Mao Jiangdi,Liu Daren,Wang Shanshan,Wang Yuhao,Wang Haifeng
Metabolic syndrome (MetS) is a difficult-to-manage disease that poses a significant risk to human health. Here, we show that the supplementation of Lactobacillus reuteri ZJ617 ameliorates symptoms of MetS in mice induced by the high-fat diet. L. reuteri ZJ617 modulates host metabolism by interacting with the microbiome, resulting in the production of spermidine synthesized by the microbiota. L. reuteri ZJ617 serves as a source of substrates for the microbiota to synthesize spermidine, hence preventing the decline of bacteria responsible for spermidine production. Spermidine treatment mimics the metabolic effects of L. reuteri ZJ617, whereas pharmacological inhibition of spermidine biosynthesis in mice abolishes these benefits. Our findings reveal the mechanism by which L. reuteri ZJ617 alleviates MetS symptoms and provide support for its potential use as a probiotic for promoting metabolic health.
研究领域:
微生物学
·
代谢生物学
·
营养学
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DOI
PubMed
高影响因子
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