JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 34
·13762-13773
·
IF 4.5
Yu Xinrong,Ma Yihang,Ma Zekun,Lian He,Meng Zhaoxu
Cuttlebone as a hemostatic traditional Chinese medicine is usually applied in powder directly to the wound for hemostasis. In order to improve the hemostatic utility of cuttlebone, gelatin/cuttlebone (Gel/Cb) membranes with different Gel/Cb ratios were fabricated through electrospinning and characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. The hemostatic effect and coagulation process of Gel/Cb electrospun membranes were evaluated in vitro by dynamic whole-blood coagulation test, in vitro hemolysis test, determination of clotting time, and platelet adhesion test and in vivo by liver and tail vein hemostasis models. It was noted that the addition of cuttlebone could significantly decrease the hemolysis rates of Gel/Cb electrospun membranes, which was only about one-sixth of the Gel electrospun membrane. The animal experiments demonstrated effective reduction in bleeding time and volume in Gel/Cb-treated mice. Specially, compared with the commercially available gauze group. Gel/Cb (7:3) electrospun membrane reduced the clotting time by 80.92% and the average bleeding volume by 50.83%. The results further indicated that Gel/Cb electrospun membranes could accelerate coagulation, possibly via the coagulation pathway activation to stimulate platelet contraction and rapid aggregation at the wound surface, providing a new method for rapid hemostasis of acute trauma. Graphical Abstract
研究领域:
生物医学工程
·
材料科学
·
血液学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Ying Huang,Zhengao Wang,Meimei Fu,Dongsong Lin,Fuyu Zhao,Keke Wu,Xia Ren,Xianjun Fu,Wuyi Zhou
Effective wound management remains a major clinical challenge, especially in preventing infections and promoting rapid healing. However, traditional wound dressings often lack multifunctionality, limiting their ability to support hemostasis and antibacterial protection. To address this challenge, in this work, a novel wound dressing was developed utilizing nano-cuttlebone particles, which are rich in calcium carbonate and chitin, to enhance hemostatic and antimicrobial functionality. The dressing, composed of polyvinyl alcohol, sodium alginate, and nano-cuttlebone, addresses the need for advanced wound care solutions. The particle size distribution of the powder was evaluated using a laser particle size analyzer, and the particle size of the nano-cuttlebone was around 75 nm. The drug release experiment showed that the drug release of nano cuttlebone hydrogel was 1.4 times that of micro cuttlebone hydrogel in 48 h. The antibacterial activity of the nano-cuttlebone-doped dressing against S. aureus was 97.32% and against E. coli was 99.99%. The cell scratch test showed that the nano-cuttlebone hydrogel gradually migrated to the central area, and its migration rate was 61.43% after 12 hours. The hydrogel also demonstrated excellent hemostatic ability in a mouse liver injury model and could rapidly stop bleeding within 1 min. In a full-thickness wound model, the nano-cuttlebone hydrogel enhanced collagen deposition and promoted faster wound closure. This study highlights the potential of marine biomaterials for developing multifunctional wound dressings to improve patient outcomes.
研究领域:
生物材料学
·
创伤修复
·
医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Fuge Niu,Chenyang Lin,Huabin Liao,Bin Zhang,Jian Zhang,Weichun Pan
In order to solve the problem of poor performance of giant squid gel, this study proposed a method to induce the aggregation of giant squid protein molecules by exogenous additives to improve its gel performance. The particle size, zeta potential, free sulfhydryl group, carbonyl group and hydrophobic index of protein solution were characterized by laser light scattering, fluorescence spectrum and ultraviolet spectrum, and the effect of egg white protein (EWP) on myofibrillar protein (MP) aggregation was analyzed. In the presence of EWP, the density and R g /R h value of protein particles decreased significantly after mixing for 600 min, indicating that the interaction between MP and EWP protein could be enhanced by embedding appropriate amount of EWP in the middle of MP protein. With the increase of the proportion of egg white protein, the blue shift of the maximum absorption wavelength of endogenous fluorescence in the mixed protein solution decreased. In the range of 25–65 °C, the change rates of surface charge, free sulfhydryl group, carbonyl group and surface hydrophobicity decreased, which proved that EWP inhibited the self-aggregation rate of MP molecules during the heating process, and induced its gel by interspersing between MP molecules to improve the formation of three-dimensional network during protein thermal gel process. Finally, the addition of EWP improved the gel properties of giant squid surimi. This study offered new direction for improving gel properties and promotes the development and application of giant squid.
研究领域:
食品科学
·
蛋白质化学
·
海洋生物资源利用
✓ 已验证
产品关联
DOI
高影响因子
Marine Drugs
·
2020
·Vol. 18
·期 10
·490
·
IF 4.073
Xuesong Yu,Qina Su,Tianqi Shen,Qiong Chen,Ying Wang,Weizhang Jia
The hydrolysate of golden cuttlefish (Sepia esculenta) was prepared by using papain, and then, it was further separated by ultrafiltration, gel filtration chromatography, and reverse-phase high-performance liquid chromatography (RP-HPLC). The peptide components of the active fraction were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and then two novel peptides, SeP2 (DVEDLEAGLAK, 1159.27 Da) and SeP5 (EITSLAPSTM, 1049.22 Da), were obtained and displayed significant alleviation effects on oxidative stress inCaenorhabditis elegans. Studies indicated thatS. esculentaantioxidant peptides (SePs) increase superoxide dismutase (SOD) activity but reduce reactive oxygen species (ROS) and malondialdehyde (MDA) levelsin oxidation-damaged nematodes. Using transgenic CF1553 nematodes, thesod-3p::GFP expression in the worms treated with SePs was significantly higher than that of the control nematodes. Real-time PCR also demonstrated that the expression of stress-related genes such assod-3is up-regulated by SePs. Furthermore, studies showed that SePs could obviously decrease fat accumulation as well as reduce the elevated ROS and MDA levels in high-fat nematodes. Taken together, these results indicated that SePs are capable of the activation of antioxidant defense and the inhibition of free radicals and lipid peroxidation, play important roles in attenuating oxidative stress and fat accumulation inC. elegans, and might have the potential to be used in nutraceutical and functional foods.Keywords:antioxidant peptides;oxidative stress;fat accumulation;Sepia esculenta;Caenorhabditis elegans
研究领域:
营养保健品
·
功能性食品
·
分子生物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Zongwang Huang,Wenjun Wang,Gaofei Zeng,Zhou Wang,Zhixi Duan,Lu Xia,Yi Zhang
Uncontrolled hemorrhage and wound healing difficulties are significant challenges in medical care. Wounds that fail to close within a limited time frame are often associated with continuous hemorrhage, hard-to-treat infections, and chronic conditions. Sepia melanin that contains eumelanin and is extracted from the ink of cuttlefish Sepia officinalis exists as agglomerates in the natural state, and it can be disassembled using kaolinite ( D -SM-Kaolin) to accelerate hemostasis and wound healing. D -SM-Kaolin fabrication using Sepia melanin cross-linked with kaolinite through dissolving, disassembling, and sedimentation, and different alkaline solvents controlled within a certain range. Hemostatic evaluation in vitro and in vivo , D -SM-Kaolin exhibited accelerated hemostatic effects, and thereinto, the blood loss takes 5 times less than contrast items. The hemostatic mechanism involves kaolinite’s intrinsic clotting cascade and facilitating blood constituents concentration at the hemorrhage site. In a normal wound model, D -SM-Kaolin also exhibited an accelerated wound healing, and more collagen formation combined with tissue cell infiltration was observed. The wound healing mechanism was attributed to the D -SM-Kaolin absorbs the excess wound exudate and accelerates the wound closure at the wound sites, besides SM’s intrinsic adhesion effect and kaolin’s intrinsic clotting cascade characteristic. These findings suggest that SM agglomeration could be eliminated using kaolinite, and thus is more beneficial to the field of hemostasis and wound healing.
研究领域:
生物材料学
·
创伤医学
·
纳米医学
·
再生医学
·
止血学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Inorganics
·
2024
·Vol. 12
·期 12
·319
·
IF 3.1
Qing Li,Penghui Liu,Huizhen Lin,Hun Xue,Jingyun Mao
The harmful effects of antibiotics on aquatic environments have become a growing concern of modern society. Developing high-performance photocatalysts capable of degrading antibiotics under solar light is, therefore, crucial. In this study, TiO2-cuttlebone composites are prepared via the sol–gel method, to produce carbonate radicals (•CO3−) under solar light irradiation. The •CO3−radicals exhibit high selectivity for the degradation of tetracycline hydrochloride (TC). Compared to TiO2alone, the TiO2-cuttlebone composite demonstrates excellent solar-driven photocatalytic activity for TC degradation in both freshwater and seawater. The reaction pathways of TC degradation in seawater are elucidated using HPLC-MS/MS analysis. Moreover, a TiO2-cuttlebone self-suspending photocatalyst device is fabricated using 3D printing technology and low-temperature deposition methods, with aluminum–plastic (AP) as a substrate. This innovative device is easily recyclable from photocatalytic solutions while maintaining high stability, making it highly desirable for practical applications.
研究领域:
环境科学
·
材料科学
·
生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Fuge Niu,Yu Jiao,Rui Zhang,Jiamei Fan,Christos Ritzoulis,Weichun Pan,Ang Li
This work studied the quantitation of myofibrillar protein isolated from giant squid by three methods (Bradford, biuret, and direct ultraviolet absorbance). The results were examined in relation to compositional size exclusion chromatography, static light scattering, zeta-potential and thermal analysis; comparisons between the apparent vs. the true protein concentration revealed the existence of disk-like nano protein particles (NPP) with a height of 2–3 nm, diameters ranging from several tens nm to 140 nm, and fractal dimensions d f of less than 1.3. In order to probe the heterogeneity of NPP particles, their properties were studied under consecutive dilutions: the d f decreased from 1.265 to 1.087, the zeta-potential increased from −4.55 mV to −1.83 mV, the denaturation temperature reduced from 63.9 °C to 58.6 °C, and the endothermic enthalpy reduced from 0.529 J s −1 to 0.362 J s −1 . In addition, ca. 90% protein molecules in solution were aggregated to form NPP.
研究领域:
食品科学
·
生物物理学
·
蛋白质化学
✓ 已验证
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2016
·Vol. 39
·期 17
·3428-3435
·
IF 2.741
Mengzhe Guo,Dengyang Yin,Jie Han,Liyan Zhang,Xiao Li,Dandan He,Yan Du,Daoquan Tang
Maltose, a common auxiliary material of pharmaceutical preparation, may disturb the analysis of total amino acids in sepia capsule by aldolization. Therefore, it is necessary to remove the maltose through a convenient method. In this work, a phenylboronic acid modified solid-phase extraction column has been synthesized and used to remove the maltose. The materials were synthesized by one step “thiol-ene” reaction and the parameters of the column such as absorption capacity, recovery, and absorption specificity have been investigated. The results showed the column (0.5 cm of length × 0.5 cm of inner diameter) can absorb 4.6 mg maltose with a linear absorption and absorption specificity. Then this technique was applied in the quantification of amino acids in sepia capsule. After the optimization of the method, four kinds of amino acids, which were the most abundant, were quantified by high-performance liquid chromatography with diode array detection. The amounts of the four kinds of amino acids are 1.5∼2 times more than that without the treatment of solid-phase extraction column, which almost overcomes the influence of the maltose. All the results indicate that the phenylboronic acid modified solid-phase extraction column can successfully help to accurately quantify the total amino acids in sepia capsule.
研究领域:
分析化学
·
药物分析
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
·
2025
·
IF 14.2
Tinghuan Wang,Rongjun Wei,Xuechun Wang,Yuanhang Yang,Zhichuang Wang,Zhenyu Wang,Zhengbin He,Songlin Yi
Thermal energy storage (TES) systems with phase change materials (PCMs) can efficiently address the intermittency and uneven distribution of solar energy. However, easy leakage, inherent rigidity, and poor photothermal conversion capacity greatly limits their practical utilization. Herein, a novel shape-stabilized composite PCMs with heat-induced flexibility and excellent photothermal conversion efficiency was prepared by impregnating a mixture of polyethylene oxide (PEO) and polyethylene glycol (PEG) into cuttlefish ink (CI) melanin decorated melamine foam (MF). To promote its long-term stability, a physical barrier of polydimethylsiloxane (PDMS) coating was applied to its surface. Finally, a series of innovative PEO/PEG@CI/MF-PDMS (PPCMP) PCMs with different concentrations of CI were successfully fabricated. The PPCMP composite PCMs possess high quality retention (94.39–97.45 %) when heated at 80 °C and flexibility when heated or light irradiated, indicating their excellent anti-leakage performance and heat-induced flexibility. And the incorporation of CI-derived melanin has endowed PPCMP composite PCMs with remarkable photothermal conversion efficiencies (83.26–93.67 %) due to its wide light absorption. More importantly, the optimized PPCMP composite exhibits a satisfactory melting enthalpy (>160 J/g) and high enthalpy efficiency (>80 %). Moreover, PDMS coating imparts the composites with outstanding hydrophobicity (WCA>100°), and PPCMP demonstrates thermal stability below 100 °C and reliability after multiple cycles. Based on the above merits, this work offers a prospective strategy for constructing shape-stabilized and flexible PCMs with excellent photothermal conversion and energy storage performance for practical applications in personal thermal management and flexible electronics.
研究领域:
材料科学
·
能源存储
·
光热转换技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Yanmin Liang,Yaru Han,Jie Dan,Runli Li,Hao Sun,Jianlong Wang,Wentao Zhang
Natural superoxide dismutase (SOD), consisting of proteins and metal cofactors, is widely used in food preservation because of its good antioxidant activity. However, due to the poor stability of SOD enzyme, its activity was reduced in the process of moving into the film, resulting in limited application. Based on the structure of the active site of the natural enzyme, Cu 2+ was used to functionalize the melanin nanoparticles (NMPs) in ink of cuttlefish, and an SOD-like nanozyme (Cu-NMPs) with high stability, high activity and strong free radical scavenging capacity was constructed. In order to apply the constructed simulated enzyme to food preservation, the simulated enzyme was embedded into carrageenan (Carr) films to prepare the composite film for food packaging. The results showed that when the concentration of Cu-NMPs was 10 μg/mL, the ·O 2 – rate could reach more than 80 %, the activity exceeded that of 60 U/mL natural SOD. In addition, the fresh-keeping test of cherry tomatoes showed that Carr/Cu-NMPs composite film extended the storage time of cherry tomatoes by more 3 days. Therefore, the present work showed that nanozymes with advanced catalytic capabilities can be constructed by metal ions and NMPs, thus successfully combined with food packaging for food preservation.
研究领域:
食品科学
·
材料科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR MATERIALS AND ENGINEERING
·
2017
·Vol. 303
·期 2
·1700407
·
IF 2.863
Qing Li,Guangfu Liao,Jun Tian,Zushun Xu
Novel fluorinated copolyimide/amine-modified sepia eumelanin (ASE) nanocomposites are successfully fabricated via covalent bonds. To achieve this, the polyimide (PI) is synthesized by random co-polycondensation. The effects of ASE on the structure and properties of the PI are investigated. A multilinked network is formed with ASE acting junctions in the nanocomposites. The mechanical properties of the PI are significantly improved by the addition of ASE, and the optimal tensile strength and elongation at break are 79.7 MPa and 85.42%, respectively. UV–vis transmittance, methylene blue (MB) photodegradation, and recyclability measurements confirm that the PI/ASE nanocomposites are transparent to visible light at low ASE loadings and show outstanding UV-shielding properties and lifetimes under intense UV irradiation owing to the synergistic absorption of UV light by the PI matrix and ASE. Furthermore, the PI/ASE nanocomposites have enhanced thermal properties with initial degradation temperatures above 500 °C. These properties endow the nanocomposites with great potential for UV-shielding in the conditions with high temperature and intense ultraviolet light.
研究领域:
材料科学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale
·
2023
·
IF 6.7
Jiameng Feng,Junjie Zou,Xiaoyu Li,Xin Du
Biomimetic design is very helpful and significant for the smart construction of micro/nanomotors with artificial intelligence. In this work, inspired by cuttlefish, who can rapidly eject poisonous ink and are also capable of fast movement to escape, we designed and fabricated a biomimetic submicromotor with the ability of simultaneous quick movement and a temperature threshold caused explosive cargo release triggered by near infra-red (NIR) light irradiation, which was approximately equivalent to the action of cuttlefish when encountering a predator. The yolk@shell structured polydopamine@mesoporous silica (PDA@MS60) with immovable and asymmetric yolk distribution was employed as a platform, and this was followed by the simultaneous encapsulation of phase change materials (PCM) and cargo molecules. The NIR light irradiation could not only propel the direct motion of the submicromotor, but also caused the explosive release of the cargo loaded in the submicromotor when the temperature exceeded the melting point of the PCM.
研究领域:
纳米技术
·
材料科学
·
生物医学工程
·
光学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）