SENSORS AND ACTUATORS B-CHEMICAL
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2016
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IF 4.758
Bixia Lin,Ying Yu,Ruoying Li,Yujuan Cao,Manli Guo
In this paper, a novel turn-on sensor was constructed for quantification and imaging of acetamiprid . Acetamiprid aptamer-modified ZnS:Mn probe (ZnS:Mn-Aptamer) was obtained by conjugating ZnS:Mn QDs with acetamiprid binding aptamer . The fluorescence of the probe was turn off by multi-walled carbon nanotubes (MWCNTs) based on fluorescence resonance energy transfer (FRET) between ZnS:Mn-Aptamer and MWCNTs. After acetamiprid was introduced, the fluorescence was turn on because ZnS:Mn-Aptamer specifically binded with acetamiprid. Based on the above-mentioned principle, the quantitative detection and imaging of acetamiprid in real samples were achieved. The linear equation could be described as F / F 0 = 1.101 + 0.0312 C A ( C A represented the concentration of acetamiprid, nM) in a linear range of 0–150 nM with a detection limit of 0.7 nM. The results suggested this method had remarkable sensitivity, selectivity , and was very simple without complex operation. Meanwhile, this novel turn-on sensor has the potential of in situ visual determination for pesticide residues in complex system.
研究领域:
分析化学
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环境科学
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农药残留检测
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Jie Zhou,Dan Wang,Haifeng Yang,Feng Wang
Acetamiprid poses a serious threat to human beings. In this work, the highly transparent fluorescent quantitative PCR sealing membranes (PCR-M) developed in our previous work which had attractive characteristics such as sensitivity, stability and reproducibility, and its capability of extracting analytes quickly, was used to detect acetamiprid. The detection limit of 1 nmol/L was obtained by direct detection of CN group in acetamiprid. In order to improve the selectivity and sensitivity, acetamiprid aptamer was introduced. After the interaction of aptamer with acetamiprid the SERS signal of adenine at 730 cm −1 increased. The detection limit of indirect detection was 10 -8 mol/L. Aptamer advanced Ag-PCR-M-based Surface enhanced Raman spectroscopy (SERS) method for indirect SERS detection which significantly improves the detection sensitivity and greatly increases the range of signal response value. This will be conducive to the detection of actual samples and expand the practical application.
研究领域:
分析化学
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食品安全检测
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
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2020
·Vol. 43
·期 20
·3921-3930
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IF 2.878
Qamar Subhani,Nadeem Muhammad,Zhouman Huang,Muhammad Asif ,Irshad Hussain,Muhammad Zahid,Cui Hairong,Yan Zhu,Dandan Guo
This study aims to introduce a simple, sensitive, and cost-effective method for the simultaneous determination of acetamiprid and its main metabolite 6-chloronicotinic acid in environmental samples by using a nonsuppressed ion chromatography hyphenated with an online postcolumn photoinduced fluorescence detection system. The fluorescence detector wavelengths λ ex /λ em = 257/382 nm was set for up to 6.0 min for acetamiprid, while λ ex /λ em = 231/370 nm programmed for 6-chloronicotinic acid for the rest of the analysis time. Both samples were treated by applying miniaturized quick, easy, cheap, effective, rugged, and safe method before the separation of analytes on an IonPac ® AS11-HC column by pumping 40 mM NaOH having minuscule content of acetonitrile (5%, v/v) as an eluent. Both intrinsically nonfluorescent analytes were turned-on by online postcolumn photoinduced derivatization, avoiding the need for complex chemical derivatization or addition of a postcolumn extra pump. The developed method was appraised for the analysis of environmental samples, exhibiting excellent linearity (0.050-10 μg/mL) with a correlation coefficient greater than 0.9993 for both analytes. Whereas, obtained limit of detection (0.025-0.0072 μg/mL), recoveries (98.02-116.00%), and inter- and intraday precision (≤3.02 %) were satisfactory for both compounds in environmental samples.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Ziwang Mao,Yibo Zhao,Jianhua Dong,Liangzhi Li,Yuyang Zhou
To explore novel and efficient ECL luminophores, a series of cationic iridium(III) complexes with 4,7-diphenyl-1,10-phenanthroline as the ancillary ligand and various main ligands were investigated in this work. The comprehensive studies demonstrate complex 1, with 2-phenylbenzothiazole (bt) as main ligand, exhibited superior ECL properties in aqueous buffer compared to other complexes. Importantly, the structurally optimized complex 1 was successfully further encapsulated into silica nanoparticles to prepare electrochemiluminescent iridium(III)-doped silica nanoparticles (Ir@SiO 2 NPs). These Ir@SiO 2 NPs were then utilized to construct a sensitive and specific ECL aptamer sensor for detecting acetamiprid residues in tea. Notably, our developed sensor demonstrated wide linear ranges from 10 pM to 10 μM and low limit of detection of 1.3 pM. These experimental results presented in this work undoubtedly demonstrate iridium(III)-doped silica nanoparticles as a new and efficient type of ECL nano-emitter have great potentials in ECL-related analytical applications in the future.
研究领域:
分析化学
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环境科学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）