FOOD CHEMISTRY
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2020
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IF 6.306
Kai Zhou,Francesca Patrignani,Yuan-Ming Sun,Rosalba Lanciotti,Zhen-Lin Xu
Ethyl carbamate (EC), a genotoxic and carcinogenic compound in soy sauce accumulated during thermal processes, has raised public health concern for its multipoint potential carcinogenic risk to human. In this work, based on the analysis of EC accumulation during thermal processes of soy sauce, ornithine and quercetin were added before thermal processes to reduce EC accumulation. A reduction rate of 23.7–63.8% in simulated solution was founded. Kinetic studies indicated that ornithine was a byproduct of alcoholysis reaction when EC formed, while quercetin could compete with the precursor ethanol and react with carbamyl compounds, which therefore prevented EC accumulation. A maximum of 47.2% decrease of EC in soy sauce was achieved, and no remarkable changes in volatile compounds profile and color of soy sauce were found. In conclusion, the addition of quercetin and ornithine before thermal processes may be preferable for the controlling of EC content in soy sauce.
研究领域:
食品科学
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毒理学
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公共卫生
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2022
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IF 7.425
Wei Jia,Chenna Di,Rong Zhang,Lin Shi
The presence of ethyl carbamate in traditional fermented food is a public health concern for the FDA. The effect of forced photoirradiation (0–150,000 lx) and thermal stress (4, 25, 40 °C) on ethyl carbamate (0–150 μg/L) on physiological characteristics of Baijiu, such as esters content, photochemical degradation, and hydrogen-bond interaction efficiency were monitored by ultra high performance liquid chromatography quadrupole-orbitrap and the dynamic changes by digital foodomics analysis. Furthermore, 748 trace components covering 11 subclasses were identified in Baijiu and 71 esters were screened by Spearman’s correlation, fold changes, P values and VIP values. A forward stepwise multiple regression and discriminant analysis were performed for predicting the content of esters from appearance characteristics obtained by foodomics analysis, reaching R-square values up to 0.91. A reduction of the present variation in ethyl lactate, ethyl caproate, diethyl succinate, ethyl oleate and ethyl linoleate concentration could possibly result in a better understanding of the ethyl carbamate effects. Ethyl carbamate was found to cause esters hydrolysis through inter-molecular interaction in various species of alcoholic drinks . It was demonstrated that the light exposure level and thermal intensity applied for ethyl carbamate-spiked Baijiu samples did not unambiguously influence esters concentration in Baijiu. Future research should focus on moderate light exposure level and thermal stress and should aim at reducing natural ethyl carbamate by more closely controlling the esters content of the ripening degree.
研究领域:
食品科学
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发酵工程
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2020
·Vol. 101
·期 8
·3156-3164
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IF 2.614
Huihui Ke,Tao Bao,Wei Chen
BACKGROUND Rubus chingii Hu is a widely cultivated fruit in China and has declared multiple bioactivities including antioxidative activity. Ethyl carbamate (EC), mostly found in fermented food and alcoholic beverages, is a recognized human carcinogen, and researchers have proposed the correlation between oxidative stress and its toxicity. This study acquired the polysaccharide from R. chingii (RP) and explored its effect on EC-induced cytotoxicity using Caco-2 cells as the cell model. RESULTS Results showed that RP exhibited protection against EC-induced toxicity by repairing redox imbalance as indicative of mitigated mitochondrial membrane potential collapse, attenuated reactive oxygen species overproduction, and impeded glutathione depletion. Moreover, the structural features of RP were characterized and revealed that it was mainly constituted by galacturonic acid and arabinose, with an average molecular weight of 7.039 × 10 5 g mol −1 . CONCLUSION Overall, our results provided a new approach dealing with the toxicity caused by EC from the perspective of oxidative stress and described a new potential healthy value of R. chingii Hu, which could contribute to the development of a promising dietary supplement and functional food. © 2020 Society of Chemical Industry
研究领域:
食品科学
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毒理学
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营养学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
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IF 4
Hongli Yang,Lian Xia,Lutao Li,Yue Tang,Linghui Huang,Han Tao,Yuangen Wu
The convenient detection of ethyl carbamate (EC) still faces some challenges due to certain interfering substances in liquor. Herein, the peroxidase-mimic activity of V 6 O 13 nanobelts was inhibited by EC1–34 aptamer, and a novel rapid method for EC detection was established using EC1–34 aptamer as a specific recognition molecule. The composite probe consisting of V 6 O 13 nanobelts and EC1–34 aptamer was immobilized on the paper to construct a colorimetric nanozyme sheet. The presence of EC1–34 aptamer would effectively inhibit the peroxidase-mimic activity of V 6 O 13 nanobelts, making the colorimetric nanozyme sheet exhibit light blue or even colorless. Once the EC was added to nanozyme sheet, the EC1–34 preferentially bound to EC molecules, restoring the peroxidase-like activity of V 6 O 13 nanobelts. The degree of the blue-gray change of the constructed colorimetric nanozyme sheets was positively correlated with the EC concentration. Moreover, a smartphone was used to assist in obtaining the image data and then drawing a standard curve. The visual limit of detection of the rapid colorimetric nanozyme sheets was 20 μg·L −1 , and the limit of detection (LOD) was calculated as 14.17 μg·L −1 . The novel rapid detection nanozyme sheets constructed in this study can be generally applied in detecting EC in liquor.
研究领域:
分析化学
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食品安全
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纳米技术
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生物传感
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产品关联
DOI
高影响因子
LETTERS IN ORGANIC CHEMISTRY
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2025
·Vol. 22
·期 8
·633-641
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IF 1
Hongyin Wang,Yu Liang,Yida Yu,Yihao Wang,Ni An,Dong Cai
The ureido moiety stands as one of the most regarded scaffolds in medicinal and organic synthesis. In this study, we endeavored to synthesize a product containing tertiary amine moieties through the nucleophilic substitution reaction of the 2-chloroethyl urea derivative with a secondary amine, utilizing either inorganic bases, such as K2CO3 or organic bases like triethylamine as acid-binding agents in diverse reaction media. Unfortunately, the reaction failed to yield the desired nucleophilic substitution product in high yield, attributed to the reactivity of the 2-chloroethyl urea derivative. The structures of potential impurities were elucidated based on comprehensive spectroscopic data, including 1H NMR, 13C NMR, HRMS, HMBC, and HSQC.
研究领域:
药物化学
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有机合成
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亲核取代反应
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化学反应优化
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DOI
高影响因子
FOOD CHEMISTRY
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2017
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IF 4.529
Yuting Li,Tao Bao,Wei Chen
Increasing evidence indicates that crude extracts derived from mulberry confer protection against oxidative stress. However, the antioxidant capacity of mulberry extract among different cultivars remains elusive. The main objective of the present study was therefore to investigate the effect of black mulberry extract (BMB) and white mulberry extract (WMB) on ethyl carbamate (EC)-induced cytotoxicity and oxidative stress. This study showed that the contents of total phenolics, total flavonoids, total procyanidins, cyanidin- 3 - O -glucoside, cyanidin- 3 - O -rutinoside and pelargonidin- 3 - O -glucoside of BMB were higher than that of WMB. Moreover, our results showed that phenolics-abundant BMB was stronger than WMB in scavenging ABTS and DPPH free radicals. BMB was more effective in ameliorating EC-induced cytotoxicity by inhibiting excessive ROS generation, suppressing mitochondrial dysfunction and increasing GSH concentration in HepG2 cells than WMB. Taken together, our study revealed that BMB afforded better protection against EC-caused cytotoxicity than WMB.
研究领域:
植物学
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毒理学
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细胞生物学
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DOI
高影响因子
TALANTA
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2024
·
IF 5.6
Huanhuan He,Jia Zheng,Jian Su,Lian Xia,Yue Tang,Yuangen Wu
A novel aptamer LFA (Apt-LFA) strip is first constructed for ethyl carbamate (EC) detection, in which cationic polyethyleneimine (PEI) and anionic polyacrylamide (APAM) are used as double-capture agents. The black NiCo 2 O 4 nanoparticles (NCO NPs) encapsulated by EC1-34 aptamer are employed as recognition probes. The added EC will bind to EC1-34 aptamer on the probes, so that the test-line (T-line) immobilized with APAM can electrostatically capture the exposed NCO NPs. The control-line (C-line) sprayed with PEI can capture the excessive black recognition probes. The peroxidase-like activity of bimetallic NCO NPs is employed as signal amplification to improve the sensitivity of Apt-LFA strip. The naked eye discernible concentration of EC is 5 μg L −1 and the detection limit (LOD) is as low as 1.36 μg L −1 . The Apt-LFA strip has the advantages of strong stability, simplicity and low cost, which provide a new method for EC detection and offer a new way for designing LFA.
研究领域:
分析化学
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纳米技术
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即时检测
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产品关联
DOI
PubMed
高影响因子
ChemSusChem
·
2021
·Vol. 15
·期 1
·e202101398
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IF 8.928
Yinghui Bian,Weichuan Jiang,Yonglei Zhang,Lishun Zhao,Xiaohang Wang,Zichuan Lv,Shuai Zhou,Yuqing Han,Hui Chen,Meng-Chang Lin
Graphical A closer look at sulfur : An Al−S cell using an AlCl 3 /acetamide electrolyte based on the oxidation of S IV shows a high working voltage of 1.70–1.95 V, whereas the cell based on the reduction of S (S x 2− ) exhibits a voltage of 0.31–1.20 V. A high-voltage Al−S cell with 0.33 mAh capacity can successfully light up a red LED (forward voltage: 1.6–2.0 V) for 2 h. This work may help in promoting the development of high-performance and low-cost Al−S batteries. Al-based batteries are promising next-generation rechargeable batteries owing to the abundance of raw materials and their high potential energy density. The Al−S system has attracted considerable attention because of its high energy density and low cost. However, its low discharge voltage plateau (0.6–1.2 V) hampers its practical application. Herein, eight ionic liquids or deep eutectic solvents were studied as electrolyte candidates for an Al−S cell. This was the first study to demonstrate that an Al−S cell based on an AlCl 3 /acetamide electrolyte (1.3 molar ratio) showed high discharge voltage plateaus (1.65–1.95 V) and a charging cut-off voltage of 2.5 V in Al−S cells. An Al−S cell of 0.33 mAh capacity with the AlCl 3 /acetamide electrolyte successfully lit up a red LED (forward voltage 1.6–2.0 V) for around 2 h. This work may help in promoting the development of high-performance and low-cost Al−S cells.
研究领域:
材料科学
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电化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）