AQUACULTURE NUTRITION
·
2026
·Vol. 2026
·期 1
·1857237
·
IF 4.6
Fan Wang,Fajun Jiang,Jia Xu,Yixin Liang,Xujia Liu,Yichao Li,Junlian Zhuang,Fangjuan Huang,Fu Lei,Chuhang Chen,Ermeng Yu,Junxiang Lai
The conjugation states of bile acids (BAs) have been demonstrated to affect BAs regulatory functions. This study aimed to investigate the effects of conjugated or non-conjugated BA additions on growth performance, liver health, and BA metabolism in pearl gentian grouper ( Epinephelus lanceolatus ♂ × E. fuscoguttatus ♀), as well as to elucidate the underlying mechanisms. The 300 healthy experimental fish, each with an average initial body weight (IBW) of 15.21 ± 0.04 g, were randomly distributed into 12 tanks (300 L per tank). The experiment consisted of four dietary groups, with three replicates per group: the control diet (CD) group, the plant protein-based diet (PD) group, the PD supplemented with conjugated BAs (CBD) group, and the PD supplemented with non-conjugated BA diet (NBD) group. The findings indicated that, in comparison to the CD group, the grouper fed a PD exhibited deleterious effects on growth performance, BA metabolism, and liver health. This was evidenced by increased levels of of oxidative stress, endoplasmic reticulum (ER) stress, apoptosis, and inflammatory response. In comparison to the PD group, the CBD treatment demonstrated favorable outcomes with respect to growth performance and liver health in the grouper. Additionally, it facilitated BA metabolism, as evidenced by enhanced BA transport, reabsorption, and recycling. Compared to PD group, the NBD treatment further impaired BA metabolism and induced cell apoptosis. Additionally, six metabolites, 3-Dehydrocarnitine, Stizolobate, Bilastine, S-(-)-Ureidoglycolate, 5,6-Dihydroxyindole, and 4-O-methylnorbelladine-, were identified as biomarkers among the treatment groups. Moreover, high plant-protein replacement and BAs addition both impacted the pathways associated with the “Biosynthesis of various plant secondary metabolites“ and “Central carbon metabolism in cancer. “In conclusion, the conjugated BAs improve growth performance, liver health, and BA metabolism in pearl gentian grouper fed a high plant-protein diet.
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高影响因子
AQUACULTURE NUTRITION
·
2020
·Vol. 26
·期 3
·954-963
·
IF 2.231
Evelina Emmanuel Wabike,Xun Wu,Wenliang Zhu,Bao Lou,Ruiyi Chen,Dongdong Xu,Ligai Wang,Shanshan Zhou,Peng Tan
The imbalance of fish oil (FO) supply and demand has motivated efforts to identify an alternative for aqua feed. An 8-week feeding trial was conducted to evaluate the effects of dietary fish oil partial replacement with mixed oil from linseed and lard (1:1) on growth, body composition and immunity when the N3 long-chain polyunsaturated fatty acid (N3 LC-PUFAs) requirement is met for Nibea albiflora . Two types of experimental diets were formulated with 100% fish oil (FO) or 69% mixed oil (Mix). The results indicated that the Mix diet significantly improved the specific growth rate (SGR), weight gain (WG), feed efficiency ratio (FER), muscle tissue growth hormone receptor ( GHR ), insulin-like growth factor 1 ( IGF1 ) and insulin-like growth factor 1 receptor ( IGF1R ) gene expression of the yellow drum ( p < .05). No significant difference in the survival rate (SR), feed intake (FI), hepatosomatic index (HSI), viscerosomatic index (VSI), condition factor (CF), N3 PUFA percentage, liver antioxidant enzyme activities or pro-inflammatory gene expression was observed between the two treatments. Therefore, terrestrial blend lipid from linseed and lard could be used as an alternative for dietary fish oil without compromising growth performance and immunity for the juvenile yellow drum.
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2018
·
IF 3.716
Haoyu Lei,Yuling Hu,Gongke Li
The novel multifunction materials for bactericides residue enrichment is attractive. Few materials used in bactericides analysis could simultaneously achieve convenient separation, recycling and trace enrichment. Therefore, a magnetic poly (phenylene ethynylene) conjugated microporous polymer (CMP) microspheres with three-dimensional network structure and covalently built-in Fe 3 O 4 nanoparticles was reported. The structure of dense poly (phenylene ethynylene) frameworks would enhance its conjugation system, which could adsorb bactericides with phenyl structure through π-π stacking. The material could be separated from sample matrix conveniently and recycled due to the super paramagnetism from embedded Fe 3 O 4 nanoparticles. After response surface optimization on extraction and desorption conditions, a method for trace analysis of six bactericides determination was developed by collaborative use of ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The detection limits were in the range of 0.27–3.1 ng / L, and bactericides had been found in vegetables and fruits at concentration among 0.038-0.62 μg/kg. Finally, the method was further applied to analyze the regression equations of bactericide dissipation under natural condition. Effects of different treatment during cleaning process of vegetables and fruits were investigated to seek for the optimal cleaning method. The results revealed that the analytical method based on magnetic CMP which could achieve convenient separation, recycling and trace enrichment, having a great potential for trace analysis of more phenyl pesticides from real samples.
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PubMed
高影响因子
Animal Nutrition
·
2024
·
IF 6.1
Jia Xu,Fan Wang,Chaoqun Hu,Junxiang Lai,Shiwei Xie,Kefu Yu,Fajun Jiang
High lipid diets (HLD) and high plant-protein diets (HPD) exhibit potential fishmeal-saving effects but negatively impact liver health and growth performance in fish. We hypothesized that HLD and HPD impair liver health in pearl gentian groupers ( Epinephelus fuscoguttatus ♂ × Epinephelus lanceolatus ♀) through the modulation of intestinal microbiota and bile acid (BA) metabolism. Four diet groups were tested: control diet (CD, 46.21% crude protein, 9.48% crude lipid), HLD (46.37% crude protein, 16.70% crude lipid), HPD (46.50% crude protein, 9.38% crude lipid), and high lipid-high plant-protein diet (HLPD, 46.54% crude protein, 16.67% crude lipid). A total of 300 fish (average body weight = 15.22 ± 0.03 g) were randomly divided into 4 diet treatments (ensuring 3 tanks replicates of each diet treatment, each tank containing 25 fish). After an eight-week feeding period, the HLD and HPD significantly decreased the final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR) and feed intake (FI) in comparison to CD group, with HLPD exacerbating these indicators ( P < 0.05). Compared to CD group, the content of total cholesterol (T-CHO) and triglyceride (TG) in liver and serum were significantly increased in HLD group ( P < 0.05). Compared to HPD group, the content of T-CHO in liver was significantly decreased, the content of TG in liver and serum were significantly increased in HLPD group ( P < 0.05). HLD, HPD, and HLPD impaired liver health by inducing histological damage, inflammation, and oxidative stress. Compared to CD group, the mRNA relative expression of bile salt export pump ( bsep ) and multidrug resistance protein 3 ( mdr3 ) were significantly increased in HLD group, whereas the mRNA relative expression of sterol-27-hydroxylase ( cyp27a1 ), microsomal epoxide hydrolase ( meh ), apical sodium-dependent bile acid transporter ( asbt ), multidrug resistance-associated protein 3 ( mrp3 ), farnesoid X receptor ( fxr ) and G protein-coupled bile acid receptor 5 ( tgr5 ) were significantly decreased ( P < 0.05). Compared to CD group, the mRNA relative expression of mdr3 , asbt , mrp3 , organic anion transporters 1 ( oatp1 ), meh , fxr and tgr5 were significantly decreased in HPD group ( P < 0.05). In summary, HLD affects intestinal microbiota, BA metabolism, and lipid metabolism, leading to lipid deposition and liver damage. HPD regulates gut microbiota, BA metabolism, inflammatory responses, and BA receptor expression, impairing grouper liver health. HLPD synergistically combines the adverse effects of HLD and HPD on grouper liver health.
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Feng He,Yang Liu,Shiyi Dai,Maozhong Sun,Liqiang Liu,Liguang Xu,Hua Kuang,Chuanlai Xu,Xinxin Xu
Ethoxyquin (EQ), widely used as a potent antioxidant in intensive agricultural, requires reliable analytical methods for rapid detection due to its toxicological risks and potential for widespread contamination. In this study, computer-assisted molecular simulations were utilized to screen for a novel hapten through conformational studies and electronic analyses. Monoclonal antibodies against EQ were prepared using hybridoma technology, exhibiting a half-maximal inhibitory concentration of 9.54 ng/mL. A gold nanoparticles-based lateral flow immunochromatographic assay (LFIA) was established for the analysis of EQ in apples, pears, and fish meat, with quantitative detection ranges of 0.03–0.42 mg/kg, 0.03–0.70 mg/kg, and 0.04–0.93 mg/kg, respectively. The spiking recoveries of LFIA for real samples ranged from 88.63 % to 106.90 %, with RSDs between 2.24 % and 7.93 %. These results align with the instrumental method, indicating that this immunoassay can accurately and efficiently detect EQ residues in foodstuffs in resource-limited areas.
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高影响因子
危险品化学品经营许可证（带存储）