MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Shuhuai Li,Chaohai Pang,Xionghui Ma,Haibo Li,Min Zhao,Chunhua Liu,Jinhui Luo
A highly sensitive molecularly imprinted ethephon sensor based on the amplification effect of gold nanoparticle (Au NPs) was designed in this study. An ethephon molecularly imprinted polymer (MIP) was prepared on a surface by electropolymerisation with Au NPs as the carriers and ethephon as the template molecule. Elution of the template molecule gave an MIP sensor that retains imprinted sites for specific recognition of ethephon. The sensor forms a current switch through adsorption of different concentrations of ethephon molecules to obtain different current responses, thereby establishing a new method for ethephon detection. The results indicated that using Au NPs as a carrier in the ethephon imprinted sensor could permit the loading of more MIP and increase the electron-transfer rate. The sensitivity of the sensor prepared with Au NPs was increased by three orders of magnitude compared to conventional MIP sensors without a carrier. Using potassium ferricyanide as a probe, the oxidation peak intensity of the probe molecule and the negative logarithm of the ethephon concentration exhibited a linear relationship within the concentration range from 1.0 × 10 −13 to 1.0 × 10 −9 mol L −1 . The linear equation was ΔI (10 −5 A) = −1.7838 × (−lg c ) + 26.1216, and the detection limit was 2.78 × 10 −14 mol L −1 . In addition, the sensor successfully measured the ethephon content in apples.
研究领域:
分析化学
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纳米科技
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植物生理学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 1
·1-10
·
IF 5.3
Zhang Zhikun,Bai Liwei,Han Jilong,Li Zhengjie,Liu Qingju
For the first time a novel fluorescent La@ZrMOF nanomaterial was synthesized for the convenient and visual detection of ethephon (ETH) based on the ligand–metal charge transfer process. The fluorescence signal gradually enhanced as the concentration of ETH increased, accompanied by a change in the color from colorless to blue. The assay can be completed within 75 min with a detection limit of 0.03 mg/L. A paper-based approach for the rapid and visual determination of ETH has also been devised, enabling the efficient used in a variety of actual products, with apples, pears, and tomatoes as examples. It proved to be simple, easy to use, and sensitive, and has the potential for further uses of ETH detection. Graphical
研究领域:
植物生理学
·
分析化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Jin Chen,Feifei Liu,Balarabe B. Ismail,Wenjun Wang,Enbo Xu,Haibo Pan,Xingqian Ye,Donghong Liu,Huan Cheng
Health-promoting anthocyanins in blood oranges can be enriched via appropriate postharvest storage conditions. Here, we explored the changes of anthocyanin accumulation and volatile profiles of Tarocco blood oranges ( Citrus sinensis L. Osbeck) treated by the low temperature alone and combined with ethephon (2 mg/mL) during 50 days of storage. The combination treatment of ethephon and low temperature (8 o C) significantly enhanced the levels of total anthocyanins (10.91 ± 0.25 mg/100 g) and individual cyanidin derivatives relative to low-temperature treatment alone. The increases of six cyanidin derivatives and three delphinidin derivatives identified by LC-MS/Q-TOF were consistent with the variation in total anthocyanins. Ethyl butanoate, ethyl 2-methylbutyrate, ethyl hexanoate, ethyl undecanoate, linalool, and d -limonene were identified as main contributors to overall aroma of fresh blood oranges. Volatile compositions and concentrations were decreased under different treatments of ethephon and temperatures, while ethephon could alleviate the loss of activity volatiles during cold storage.
研究领域:
园艺学
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食品科学
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植物生理学
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DOI
PubMed
高影响因子
Journal of Science-Advanced Materials and Devices
·
2024
·
IF 6.7
Zhikun Zhang,Liwei Bai,Tianyi Liu,Huanshu Zhang,Zhengjie Li,Qingju Liu,Jilong Han
Ethephon (ETH) is a plant growth regulator extensively utilized in agriculture, but its overuse is associated with several health issues. However, detecting ETH sensitively and easily in the field is challenging due to the complexity and time-consuming operation of current approaches. In this study, we synthesized aluminum-based metal-organic frameworks (CAU-1) as fluorescent probes for the detection of ETH in fruit. ETH exposure enhanced and shifted the light green fluorescence of CAU-1 to blue through high absorption of phosphate by electrostatic attraction. We observed a linear correlation between fluorescent intensity and ETH concentration ranging from 2 mg/L to 400 mg/L, with the lowest detection limit at 1 mg/L. This fluorescent analysis exhibited good selectivity towards ETH. To facilitate easy identification of samples in the field, we designed a fluorescent paper-based platform with a logic gate operation, allowing visual distinction of samples containing ETH. We applied this platform to monitor ETH in various fruits, including apples, pears, and tomatoes, through fluorescent spectrum, and visual detection. The platform offered simplicity, speed, ease of use and sensitivity, providing a promising future for ETH detection in agriculture.
研究领域:
农业科学
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分析化学
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植物生理学
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DOI
高影响因子
JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
·
2018
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IF 0.715
Ti Wu,Shoukai Lin,Bisha Wu,Yu Chen,Suying Lin,Dahe Lin,Linxia Chen,Zhisheng Zhang,Jincheng Wu
Cold-stored fruits of the loquat (Eriobotrya japonica Lindl.) plant suffer from strong lignification, which leads to a hard texture and a crude mouthfeel of the pulp. However, the signalling mechanisms of loquat fruits under cold storage remain unclear. In this study, the heterotrimeric G protein α subunit (Gα) gene (EjLGA1) from loquat was isolated, and in silico analysis was performed. The levels of EjLGA1 expression in cold-stored loquat fruits with different pretreatments were also investigated. The largest open-reading frame of EjLGA1 is 1173 bp, encoding a polypeptide of 390 amino acids. The nucleotide and amino acid sequences of the Gα subunit are highly conserved among loquat and 48 other seed plants, indicating that the loquat Gα subunit likely has similar functions in other plants. A reliable model of the loquat Gα subunit was constructed, which revealed the structure of the subunit. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) results showed that EjLGA1 is highly expressed under cold storage, which suggests the significance of EjLGA1 function in loquat fruits in response to cold stress. However, ethephon and 1-methylcyclopropene (1-MCP) pretreatments did not regulate EjLGA1expression. These results provide new insight into the signalling mechanism in cold-stored loquat fruits.
研究领域:
植物学
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分子生物学
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食品科学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Qianqian Liu,Rongrong Huang,Jiaqi Tang,Helan Zhang,Mei Liu,Yu Fang
Ethephon (ETH) is widely used to promote fruit ripening and improve fruit quality. However, improper use is harmful to human health and to the environmental safety. Therefore, development of the techniques for on-site and at real-time monitoring of ETH is of importance for its safe use. In this work, we developed a nanofilm-based fluorescence film sensor (FFS) and realized highly efficient detection of ETH in vapor phase, where the detection limit (DL) is <0.2 ppb, the response time is less than 10 s, and the interference is almost free. The unusual sensing performance of the sensor was ascribed to the specific binding of the nanofilm to ETH and to its great porosity, which enables efficient adlayer mass transfer, a requirement for high signal-to-noise ratio. Moreover, visualization-based qualitative sensing is also realized. The nanofilm, a key component of the sensor, was prepared at the humid air/DMSO interface. The building blocks used were a specially designed fluorescent o-carborane derivative (CB-2CHO) and a cross-linker BTN possessing three acylhydrazine groups. The nanofilm as prepared is flexible, uniform, thickness tunable, and photochemically super stable. We believe our effort not only addresses the challenging issue of on-site and at real-time detection of ETH but also provides another route for developing new FFSs via sensing film innovation.
研究领域:
分析化学
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材料科学
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环境科学
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食品安全
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DOI
PubMed
高影响因子
CHEMOSPHERE
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2023
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IF 8.8
Hui Zhao,Xiangyu Liu,Yulong Sun,Juan Liu,Michael Gatheru Waigi
A vast number of bacteria occur in both soil and plants, with some of them harboring antibiotic resistance genes (ARGs). When bacteria congregate on the interface of soil particles or on plant root surfaces, these ARGs can be transferred between bacteria via conjugation, leading to the formation of antibiotic-resistant pathogens that threaten human health. Plant growth regulators (PGRs) are widely used in agricultural production, promoting plant growth and increasing crop yields. However, until now, little information has been known about the effects of PGRs on the horizontal gene transfer (HGT) of ARGs. In this study, with Escherichia coli DH5α (carrying RP4 plasmid with Tet R , Amp R , Kan R ) as the donor and E. coli HB101 as the recipient, a series of diparental conjugation experiments were conducted to investigate the effects of indoleacetic acid (IAA), ethel (ETH) and gibberellin (GA 3 ) on HGT of ARGs via plasmid-mediated conjugation. Furthermore, the mechanisms involved were also clarified. The results showed that all three PGRs affected the ARG transfer frequency by inducing the intracellular reactive oxygen species (ROS) formation, changing the cell membrane permeability, and regulating the gene transcription of traA , traL , trfAp , trbBp , kilA , and korA in plasmid RP4. In detail, 50–100 mg⋅L −1 IAA, 20–50 mg⋅L −1 ETH and 1500–2500 mg⋅L −1 GA 3 all significantly promoted the ARG conjugation. This study indicated that widespread use of PGRs in agricultural production could affect the HGT of ARGs via plasmid-mediated conjugation, and the application of reasonable concentrations of PGRs could reduce the ARG transmission in both soil environments and plants.
研究领域:
微生物学
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植物科学
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环境科学
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分子生物学
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DOI
PubMed
高影响因子
PLANT PATHOLOGY
·
2014
·Vol. 63
·期 6
·1306-1315
·
IF 2.969
Z. Li,W. Zhu,Y.-C. Fan,J.-L. Ye,G.-H. Li
Peach gummosis, caused by Botryosphaeria spp. fungi, is the process of gum accumulation and exudation in plants. Ethephon (2-chloroethylphosphonic acid) has profound effects on plants, including enhanced production of secondary metabolites and regulation of plant diseases. This study investigates the effects of application of ethephon before and after inoculation with Lasiodiplodia theobromae on gum formation. Gum formation was promoted by ethephon treatment prior to pathogen inoculation, but inhibited by ethephon applied after the pathogen. The inhibitory effect was counteracted by 1-methylcyclopropane, which is an ethylene signal inhibitor. 1-methylcyclopropane also promoted gum formation. Exposure of three isolates of Botryosphaeria to ethephon inhibited mycelial growth. Both treatment methods increased the sugar content at 12 and 24 h post-inoculation (hpi). However, the sucrose, glucose and fructose contents were significantly higher in shoots with ethephon post-treatment (application of ethephon after the pathogen inoculation) than those in shoots with ethephon pre-treatment (application of ethephon prior to pathogen inoculation) at 48 and 72 hpi. The expression of two putative senescence-related genes, SEN2 and SEN4 , were significantly enhanced in pre- and post-treated shoots with ethephon at 24, 48 and 72 hpi. Ethephon application also up-regulated expression of the pathogenesis-related protein PR4 while down-regulating PR1a and PR10. The results show that ethephon has a dual function in regulating gum formation by affecting both the peach shoots and the pathogen.
研究领域:
植物病理学
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分子生物学
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植物生理学
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DOI
高影响因子
ANALYTICAL LETTERS
·
2025
·
IF 1.8
Yunna Li,Fan Qi,Jinsong Liu,Yunfei Zhao,Jiale Fang,Xueliang Li,Kezhi Jiang
Ethephon (ETH) is a widely used plant growth regulator in fruit production. Due to its harm to people’s health, its content is an important indicator for fruit quality control. However, due to the extremely high polarity and acidity, as well as the absence of chromophores, efficient and simple detection of ETH remains challenging. In this work, a convenient method for determining trace ETH in fruit was developed that involved a combination of catalytic methylation and gas chromatography-mass spectrometry (GC-MS) analysis. ETH in fruit was extracted with acetonitrile and derivatized with (trimethylsilyl) diazomethane (TMSD) by the catalysis of trimethylchlorosilane to generate methylated ETH (ETH-M) which was determined by GC-MS. The instrumental linear range of ETH was 10–1000 µg/L with a coefficient of determination (R2) > 0.999. The limit of detection (LOD) of the method was 1.0 µg/kg. The ETH content in 12 types of fruits was determined with a reasonable recovery rate ranging from 83.20 ± 0.05% to 118.21 ± 0.01%. The ETH content in the unwashed fruits was in the range of undetectable (ND) to 111 µg/g and below the LOD in their flesh. After washing, the content was significantly reduced and even undetectable. The established method showed good efficiency, selectivity, and reproducibility, and can be used for the determination of ETH in various fruits.
研究领域:
分析化学
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食品安全
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植物生长调节剂
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环境与农业化学
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产品关联
DOI
高影响因子
Frontiers in Plant Science
·
2024
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IF 4.1
Cai Zeping,Dai Yinuo,Jin Xia,Xu Hui,Huang Zhen,Xie Zhenyu,Yu Xudong,Luo Jiajia
Plant roots are constantly prepared to adjust their growth trajectories to avoid unfavorable environments, and their ability to reorient is particularly crucial for survival. Under laboratory conditions, this continuous reorientation of the root tip is manifested as coiling or waving, which we refer to as root circumnutation. However, the effect of ambient temperature (AT) on root circumnutation remains unexplored. In this study, rice seedlings were employed to assess the impact of varying ATs on root circumnutation. The role of ethylene in mediating root circumnutation under elevated AT was examined using the ethylene biosynthesis inhibitor aminooxyacetic acid (AOA) and the ethylene perception antagonist silver thiosulfate (STS). Furthermore, transcriptome sequencing, weighted gene co-expression network analysis, and real-time quantitative PCR were utilized to analyze gene expressions in rice root tips under four distinct treatments: 25°C, 35°C, 35°C+STS, and 35°C+AOA. As a result, genes associated with ethylene synthesis and signaling (OsACOs and OsERFs), auxin synthesis and transport (OsYUCCA6, OsABCB15, and OsNPFs), cell elongation (OsEXPAs, OsXTHs, OsEGL1, and OsEXORDIUMs), as well as the inhibition of root curling (OsRMC) were identified. Notably, the expression levels of these genes increased with rising temperatures above 25°C. This study is the first to demonstrate that elevated AT can induce root circumnutation in rice via the ethylene pathway and proposes a potential molecular model through the identification of key genes. These findings offer valuable insights into the growth regulation mechanism of plant roots under elevated AT conditions.
研究领域:
植物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Jialiang Liu,Tongtong Liang,Yue Xiang,Benfeng Zhang,Lisha Zhu,Xiangbin Xu,Xuequn Shi,Zhengke Zhang
Starch degradation is a fundamental process underlying the development of key postharvest quality attributes in ripening mango fruit; however, its regulatory mechanisms remain incompletely understood. In this study, ethephon (ETH) and 1-methylcyclopropene (1-MCP) were used as ripening modulators to investigate the patterns of starch degradation and its transcriptional regulation in postharvest mango fruit. The results demonstrated that ETH fumigation accelerated the ripening process, while 1-MCP treatment effectively inhibited this process. Transcriptome and RT-qPCR analyses revealed and validated that six starch-degrading genes (MiAMY1.1, MiAMY3, MiBAM3, MiBAM9, MiGWD and MiPWD) were regulated in response to ETH. Furthermore, the transcription factor MiRAP2.11 was identified as a nucleus-localized transcriptional activator of MiBAM3 and MiPWD. Transient transformation assays demonstrated that MiRAP2.11 positively regulated starch degradation, thereby promoting development of ripening and quality attributes. The results indicate that MiRAP2.11 functions as a crucial regulator mediating ethylene-induced starch degradation during mango ripening.
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PubMed
高影响因子
Horticulturae
·
2025
·Vol. 11
·期 11
·1280
·
IF 3
Renchi Chen,Yuhua Yan,Weiliang Guan,Zhihai Huang
Both abscisic acid (ABA) and ethylene play essential roles in the ripening process of strawberry fruit (Fragaria×ananassaDuch.). In this study, the crosstalk between ABA and ethylene involved in strawberry fruit ripening was investigated. The results showed that ethylene triggered a 1.30-fold increase in ABA levels by suppressing the expression of miR396. Meanwhile, the silencing of miR396 (STTM396 reporter) demonstrated that miR396 acts as a negative regulatory factor of ABA biosynthesis by repressingFaNCED2expression. Additionally, dual-luciferase assays indicated a 0.56-fold suppression ofFaNCED2promoter activity by miR396. These findings reveal a novel regulatory mechanism in which ethylene promotes ABA biosynthesis through the suppression of miR396 expression in postharvest strawberry fruit.
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高影响因子
危险品化学品经营许可证（带存储）