JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Xutian Zheng,Chaolun Liu,Jiye Hu
Since 2,4-dichlorophenoxy acetic acid (2,4-D) was discovered in the 1940s, 2,4-D and its derivatives remain among most commonly used herbicides in the world. There have been recent increases in using 2,4-D products in a combination with other herbicides such as metribuzin and acetochlor to control noxious weeds. However, accurate analysis of 2,4-D isooctyl ester remains to be improved due to long analysis time and rapid conversion of the ester to acid (i.e., under-reporting residues). In this work, a simple hydrolysis procedure was introduced to provide a quantitative hydrolytic rate of the ester (>95%) and did not affect the other pH-sensitive compounds. Analysis parameters and sample pretreatments were optimized for improved selectivity and accuracy. The hydrolysis-QuEChERS (quick, easy, cheap, effective, rugged, and safe) technique for multidetermination of 2,4-D isooctyl ester, metribuzin, acetochlor, and 2-ethyl-6-methylaniline in corn and soybeans via high performance liquid chromatography–tandem mass spectrometry was established. The method had average recoveries of 74–109% with relative standard deviations ≤13.5% and limits of quantifications (LOQs) of 0.05 mg/kg. The terminal residues of these compounds found in real edible matrixes were less than the corresponding LOQs at harvest time. The risk quotients were far below 100%, indicating a low health risk to consumers.
研究领域:
植物保护
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食品安全
·
环境科学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Xin Wang,Fan Chen,Jian Lu,Mengqi Wu,Jiagao Cheng,Wenping Xu,Zhong Li,Yang Zhang
Acetochlor (ACT) is a widely used pesticide, yet the environmental and health safety of its chiral isomers remains inadequately evaluated. In this study, we evaluated the toxicity of ACT and its chiral isomers in a zebrafish model. Our findings demonstrate that ACT and its chiral isomers disrupt early zebrafish embryo development, inducing oxidative stress, abnormal lipid metabolism, and apoptosis. Additionally, ACT and its chiral isomers lead to cardiovascular damage, including reduced heart rate, decreased red blood cell (RBC) flow rate, and vascular damage. We further observed that (+)- S -ACT has a significant impact on the transcription of genes involved in cardiac and vascular development, including tbx5 , hand2 , nkx2.5 , gata4 , vegfa , dll4 , cdh5 , and vegfc . Our study highlights the potential risk posed by different conformations of chiral isomeric pesticides and raises concerns regarding their impact on human health. Overall, our results suggest that the chiral isomers of ACT induce developmental defects and cardiovascular toxicity in zebrafish, with (+)- S -ACT being considerably more toxic to zebrafish than (−)- R -ACT.
研究领域:
环境毒理学
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发育生物学
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心血管科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
CONSTRUCTION AND BUILDING MATERIALS
·
2025
·
IF 8
Yujie Wang,Xili Yan,Kenan Li,Siqi Li,Tao Ai,Fengrong Shi
Conventional epoxy asphalt is difficult to reuse due to its thermosetting inability to heal and reprocess. In this study, using dimer acid (DA) and zinc acetate (Zn(OAC) 2 ) as curing agent and catalyst, respectively, and the hydroxyl-ester bonds were introduced into the dynamic crosslinking network system of the asphalt, a highly compatible epoxy asphalt DEA with heat recovery performance was designed. On this basis, the effects of different contents of capped hyperbranched polyester HP102 on the mechanical properties, low-temperature properties, and compatibility of DEA were investigated. The results showed that the base asphalt was successfully grafted with hydroxyl and ester groups, and this chemical modification effect reduced the polarity between the asphalt phase and the resin phase, resulting in a notable improvement in the compatibility of DEA compared with commercially available epoxy asphalt. The introduced HP102 improves not only the low-temperature ductility of the material but also its structural presence of -COO- groups, which can be further used to realize the dynamic exchange and reorganization of hydroxyl-ester bonds, which contributes to the modulus relaxation of the material and enhances its mechanical properties and self-healing ability. The highly branched structure of HP102 increases the unoccupied space and undergoes stress relaxation, which contributes to the improvement of the low-temperature ductility of the material. In addition, the cyclic tensile test results show that the mechanical property recovery rate can reach 86.7 % when hot-pressing processed at 180 °C for 5 h, which confirms that the epoxy asphalt under the reversible covalent crosslinking network possesses non-negligible recycling potential, and provides an engineering application reference for the hot-pressing recycling of epoxy asphalt.
研究领域:
材料科学
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化学工程
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道路工程
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Qiaolin Li,Chen-Yu Zhou,Huiling Wang,Hong-Tao Shao,Shi-Yu Liu
Hepatic carcinoma is one of the most common malignancies with low survival rates due to insufficient therapeutics while early detection of hepatic tumors remains key to reducing mortality of liver cancer. However, current clinical methods are hard to detect pathological changes in early-stage cases while the detection rate of liquid biopsy-based assay is limited. Thus, effective tools for accurate and sensitive detection of hepatic tumors are still in urgent need. To date, fluorescent probes targeting cancer-related biomarkers and cellular environments have attracted intense attention, among which “dual-locked” probes have developed to avoid false-positives and have shown great potential in accurate detection of tumors. Herein, carboxylesterase (CE), a widely expressed serine hydrolase that shows high relevance with hepatic carcinoma progression and tumor-associated cellular viscosity were chosen as co-targets for hepatic tumor targeting probe development. By rational design and construction of a “dual-locked” probe for simultaneously sensing carboxylesterase activity and cellular viscosity, the newly acquired probe DCI-CV displayed high specificity and sensitivity toward CE in both in vitro assays and cell imaging. Most importantly, DCI-CV was successfully applied to imaging subcutaneous and orthotopic transplanted hepatic tumors with high specificity, demonstrating DCI-CV as a potential tool in hepatic tumor detection and related surgical navigation.
研究领域:
生物化学
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分子成像
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癌症研究
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2024
·
IF 3.8
Mingzhe Jiang,Hao Tan,Lixin Jiang,Jintang Duan,Xueping Gu,Lianfang Feng,Cailiang Zhang
The anionic ring-opening polymerization of ε-caprolactam (CL) offers significant potential for producing polyamide 6 (PA6). To establish optimal conditions for designing an efficient and continuous PA6 production process, this study examines the anionic polymerization of CL at temperatures above the crystallization point of PA6. The focus is on the effects of the temperature, activator type and concentration, and catalyst concentration on the polymerization process and product characteristics. At 220 °C, the use of bifunctional methylene diphenyl diisocyanate (MDI) as an activator initiates cross-linking reactions, producing insoluble, nonmelting PA6. In contrast, anionic polymerization without an activator prevents gel formation but results in uncontrollably high molecular weights. The introduction of monofunctional N-acetyl caprolactam (AcCL) as an activator eliminates cross-linking reactions at temperatures above 220 °C while allowing precise control of the product’s molecular weight through adjustments in AcCL concentration.
研究领域:
高分子化学
·
材料科学
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产品关联
DOI
高影响因子
Pharmaceutics
·
2025
·Vol. 17
·期 3
·376
·
IF 5.5
Yuxing Zhang,Xixi Zou,Qiran Du,Xiaotao Dong,Uday Kumar Chinta,Ruyue Yu,Fei Wu,Tuo Jin
Objectives: Thermo-gelling hydrophilic polymers like PLGA–PEG–PLGA are known as injectable sustained-release depots for biologics, but they face challenges due to the occurrence of severe burst release. This study aimed to develop a strategy to avoid the initial burst release by pre-encapsulating proteins in polysaccharide microparticles through an aqueous–aqueous emulsion mechanism, thereby enhancing therapeutic retention and linear release kinetics.Methods: Five model proteins (G-CSF, GM-CSF, IGF-1, FVIII, BSA) were encapsulated in dextran microparticles, using an organic solvent-free aqueous–aqueous emulsion method. These particles were dispersed in a 23% (w/w) PLGA–PEG–PLGA solution and injected into a 37 °C release buffer to form a gel depot. The in vitro release profiles were quantified using ELISA and MicroBCA assays over 9–42 days. The bioactivity of the proteins was validated using cell proliferation assays (NFS-60, TF-1, MCF-7) and chromogenic kits. The in vivo pharmacokinetics of the FVIII-loaded formulations were evaluated in Sprague–Dawley rats (n = 5/group) over 28 days.Results: Protein-loaded dextran particles retained their structural integrity within the hydrogel and exhibited minimal burst release (≤5% within 30 min vs. >25% for free proteins). Sustained near-linear release profiles were observed for all the proteins, with complete release by day 9 (G-CSF, GM-CSF, BSA) or day 42 (FVIII). Rats administered with the thermal gel with FVIII–dextran particles showed a significantly lower peak plasma concentration (Cmax: 88.25 ± 30.21 vs. 132.63 ± 66.67 ng/mL) and prolonged therapeutic coverage (>18 days vs. 15 days) compared to those administered with the thermal gel with the FVIII solution. The bioactivity of the released proteins remained at ≥90% of the native forms.Conclusions: Pre-encapsulation in dextran microparticles effectively mitigates burst release from thermosensitive hydrogels, while preserving protein functionality.
研究领域:
药剂学
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生物医学工程
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药物递送系统
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蛋白质工程
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产品关联
DOI
PubMed
高影响因子
SENSORS
·
2025
·Vol. 25
·期 2
·407
·
IF 3.5
Yingzhen Zhang,Jun Xiao,Ruiying Peng,Xueliang Feng,Haimei Mao,Kunming Liu,Zhenzhong Liu,Chunxin Ma
The detection of highly toxic chemicals such as phosgene is crucial for addressing the severe threats to human health and public safety posed by terrorist attacks and industrial mishaps. However, timely and precise monitoring of phosgene at a low cost remains a significant challenge. This work is the first to report a novel fluorescent system based on the Intramolecular Charge Transfer (ICT) effect, which can rapidly detect phosgene in both solution and gas phases with high sensitivity by integrating a benzo[1,2-b:6,5-b’]dithiophene-4,5-diamine (BDTA) probe. Among existing detecting methods, this fluorescent system stands out as it can respond to phosgene within a mere 30 s and has a detection limit as low as 0.16 μM in solution. Furthermore, the sensing mechanism was rigorously validated through high-resolution mass spectrometry (HRMS) and density functional theory (DFT) calculations. As a result, this fluorescent probing system for phosgene can be effectively adapted for real-time, high-sensitivity sensing and used as a test strip for visual monitoring without the need for specific equipment, which will also provide a new strategy for the fluorescent detection of other toxic materials.
研究领域:
化学
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环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials
·
2021
·Vol. 14
·期 7
·1596
·
IF 3.623
Peng Zhang,Yongqi Zhang,Xuan Wang,Jiaming Yang,Wenbin Han
Blending thermoplastic elastomers into polypropylene (PP) can make it have great potential for high-voltage direct current (HVDC) cable insulation by improving its toughness. However, when a large amount of thermoplastic elastomer is blended, the electrical strength of PP will be decreased consequently, which cannot meet the electrical requirements of HVDC cables. To solve this problem, in this paper, the inherent structure of thermoplastic elastomer SEBS was used to construct acetophenone structural units on its benzene ring through Friedel–Crafts acylation, making it a voltage stabilizer that can enhance the electrical strength of the polymer. The DC electrical insulation properties and mechanical properties of acetylated SEBS (Ac-SEBS)/PP were investigated in this paper. The results showed that by doping 30% Ac-SEBS into PP, the acetophenone structural unit on Ac-SEBS remarkably increased the DC breakdown field strength of SEBS/PP by absorbing high-energy electrons. When the degree of acetylation reached 4.6%, the DC breakdown field strength of Ac-SEBS/ PP increased by 22.4% and was a little higher than that of PP. Ac-SEBS, with high electron affinity, is also able to reduce carrier mobility through electron capture, resulting in lower conductivity currents in SEBS/PP and suppressing space charge accumulation to a certain extent, which enhances the insulation properties. Besides, the highly flexible Ac-SEBS can maintain the toughening effect of SEBS, resulting in a remarkable increase in the tensile strength and elongation at the break of PP. Therefore, Ac-SEBS/PP blends possess excellent insulation properties and mechanical properties simultaneously, which are promising as insulation materials for HVDC cables.Keywords:polypropylene;voltage stabilizer;HVDC cable;thermoplastic elastomer
研究领域:
材料科学
·
电气工程
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产品关联
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2021
·
IF 4.939
Chao Li,Yao Lu,Lili Cheng,Xiaoge Zhang,Jun Yue,Jie Liu
As a noninvasive therapy, high-intensity focused ultrasound (HIFU) shows great potential in inducing anticancer immune responses. However, the overall anticancer efficacy of HIFU is still limited due to the rapid attenuation of ultrasound waves and inadequacy of ultrasound waves to spread to the whole tumor. Here, we combined HIFU with the ultrasound contrast agent/chemotherapeutic drug co-delivery nanodroplets to achieve synergistic enhancement of anticancer efficacy. Different from the widely used thermal HIFU irradiation, by which excessive heating would result in inactivation of immune stimulatory molecules, we used short acoustic pulses to trigger HIFU (mechanical HIFU, mHIFU) to improve anticancer immune responses. The nanodroplets displayed a mHIFU/glutathione (GSH)-dual responsive drug release property, and their cellular uptake efficacy and toxicity against cancer cells increased upon mHIFU irradiation. The generated immunogenic debris successfully induced the exposure of damage-associated molecular patterns on the cell surface for dendritic cells (DCs) maturation. In vivo experiments with tumor-bearing mice showed that the co-delivery nanodroplets in combination with mHIFU could effectively inhibit tumor growth by inducing immunogenic cell death, activating DCs maturation, and enhancing the effector T-cell infiltration within tumors. This work reveals that combined treatment with nanodroplets and mHIFU is a promising approach to eradicate tumors.
研究领域:
肿瘤学
·
生物医学工程
·
免疫治疗
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Fangbo Xia,Ruibing Feng,Feng-Guo Xu,Huanxing Su,Chengwei He,Yuan-Jia Hu,Jian-Bo Wan
Phospholipid fatty acids play the crucial role in biophysical properties and the function of cellular membranes. In the present study, an accurate and sensitive method was developed to quantify phospholipid fatty acids in biological samples by using chemical isotope labeling coupled with atmospheric pressure gas chromatography quadrupole-time-of-flight mass spectrometry (APGC/Q-TOF MS). APGC, a soft ionization source, was operated under proton-transfer condition by introducing methanol into the ionization source as a modifier, which provided high quantifiable molecular ion peaks to substantially enhance the sensitivity. Fatty acid standards were methylated with methanol- d 4 to yield FAMEs- d 3 that were used as one-to-one internal standards to ensure accurate quantification. Thirty fatty acids in phospholipids were accurately quantified in wide linear range with limit of quantification ranging from 84.6 to 113.2 pg/mL. The newly developed method was successfully applied to quantify phospholipid fatty acids in brain and liver tissues from both fat-1 and WT mice. This method might be expanded to quantify free fatty acids or other conjugated fatty acids in biological samples or other matrices.
研究领域:
生物化学
·
分析化学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2021
·
IF 3.196
Xin Feng,Xiaolin Yang,Meitong Li,Yujie Qin,Haiqing Li,Yuhong Xie
Polystyrene (PS), when used as a substrate, reacts with acetyl chloride through the Friedel–Crafts mechanism to yield fluorescent acetylated polystyrene. Different acetylated polystyrene microspheres can be obtained under different experimental conditions. The fluorescence of acetylated polystyrene is observed via fluorescence microscopy, while the quantum yield of acetylated polystyrene is determined using a steady-state transient fluorescence spectrometer. The surface morphology is observed via scanning electron microscopy, and the chemical composition and structure are investigated by Fourier infrared spectroscopy, ultraviolet spectrophotometry, X-ray diffraction, and nuclear magnetic resonance spectroscopy. Finally, the optimal reaction conditions are determined as follows: carbon disulfide (CS 2 , 35 mL), aluminium chloride (AlCl 3 , 1.161 g), acetyl chloride (AC, 1.171 g), soaking time of 12 h, temperature of 50 °C, and water bath for 5 h. Under these conditions, the acetylated polystyrene exhibited obvious fluorescence, with good fluorescence stability and coverage. Furthermore, it exhibited acid and alkali resistance, low-temperature resistance, and light resistance, and did not produce fluorescence leakage. In addition, it could be used as a tracer in organisms.
研究领域:
材料科学
·
化学工程
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Guojian Yang,Baige Yang,Weijia Mu,Yinghao Ge,Yiru Cai,Zhiqiang Yao,Zhaoyu Ma,Yu-Mo Zhang,Sean Xiao-An Zhang
Due to the high energy efficiency and a wide range of potential applications in daily life, bistable electrochromic devices (BECDs) have gained extensive attention in recent years. However, poor bistability and slow response rate are the main barriers, which restrict the development of BECDs. Herein, a transparent multidimensional electrode with indium tin oxide (ITO) nanofibers and gold nanoparticles was fabricated to improve both the bistability and responsiveness of devices. Its criss-crossed ITO nanofibers with high length-to-diameter ratios to reduce the distance of heterogeneous electron transfer and its gold nanoparticles to form a stable Au–S bond to avoid self-erasing of electrochromic materials have been demonstrated and discussed systematically. This device based on the transparent multidimensional working electrode had a shorter response time (1/5) and a longer bistability (12/1) compared with the prototype devices based on common fluorine-doped tin oxide electrodes. Besides, this device had a promising electrochromic performance with a high coloration efficiency of 448.27 cm2/C and a switching stability with no significant attenuation after 1200 cycles.
研究领域:
材料科学
·
纳米技术
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）