INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
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2026
·Vol. 57
·期 4
·1-13
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IF 8.5
Peng-Yao Lin,Jia-Rong Xie,Tian-Chen Qian,Shi-Song Wang,Si-Yi Yu,Wen-Bo Shi,Ying Wang,Lu-Ze Cen,Qing-Jing Zhu,Yi-Yang Zheng,Hui Gao,Rong Fang,Zhao-Xia Xia,Ai-Ming Liu,Lei Xu
International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Siting Peng,Wendi Xiao,Liwei Gao,Junyuan Shi,Dingfeng Wang,Junjie Pan,Houwen Hu,Da Chen
Flavonoids, widely recognized for their health benefits, are commonly consumed through plant-based diets. However, excessive intake of these bioactive compounds may pose significant health risks. Thus, reliable detection methods for assessing their safety are urgently required. In this work, an innovative ratiometric fluorescence method based on dual-excitation carbon dots (DE-CDs) is proposed for the simultaneous detection and discrimination of flavonoids, including quercetin, rutin, morin and kaempferol. The ratiometric signal, derived from the intensity ratio of two excitations at a fixed emission wavelength, exhibits a correlated change in response to varying flavonoid concentrations. As a proof of feasibility, quercetin is selected as a model analyte, achieving a broad linear range of 0.075–50 μM with a low detection limit of 75 nM. Furthermore, the absorption bands of flavonoids and the excitation spectrum of DE-CDs display differential spectral superposition, leading to variations in the internal filtration effect efficiency, thus enabling the discrimination of different flavonoids even in mixed samples. Additionally, to enhance practical applicability, a smartphone-assisted sensing device is designed by incorporating a DE-CDs-embedded polyvinyl alcohol film, which successfully detects flavonoids via RGB analysis and provides satisfactory recovery rates in real-sample assays. Collectively, this study not only establishes a sensitive excitation-encoded ratiometric fluorescence approach for the detection and specific discrimination of flavonoids but also demonstrates its potential for on-site applications through integration with smartphone technology.
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Biology-Basel
·
2021
·Vol. 10
·期 6
·548
·
IF 5.079
Ferry Saputra,Yu-Heng Lai,Rey Arturo T. Fernandez,Allan Patrick G. Macabeo,Hong-Thih Lai,Jong-Chin Huang,Chung-Der Hsiao
Simple SummaryThe usage of artificial sweetener has been increased from year to year as the result of pursuing healthy lifestyle. However, ironically, several studies suggest that the consumption of artificial sweeteners cause sugar-related adverse effects (e.g., obesity, type 2 diabetes and cardiovascular disease). In this study, we explore the potential cardiovascular adverse effect of several artificial sweeteners using zebrafish as animal model. We found that artificial sweetener at the highest concentration found in nature only slightly alter the cardiovascular performance of zebrafish larvae. Furthermore, no alteration of cardiac performance showed after longer incubation which support the safety of artificial sweeteners.AbstractArtificial sweeteners are widely used food ingredients in beverages and drinks to lower calorie intake which in turn helps prevent lifestyle diseases such as obesity. However, as their popularity has increased, the release of artificial sweetener to the aquatic environment has also increased at a tremendous rate. Thus, our study aims to systematically explore the potential cardiovascular physiology alterations caused by eight commercial artificial sweeteners, including acesulfame-K, alitame, aspartame, sodium cyclamate, dulcin, neotame, saccharine and sucralose, at the highest environmentally relevant concentration on cardiovascular performance using zebrafish (Danio rerio) as a model system. Embryonic zebrafish were exposed to the eight artificial sweeteners at 100 ppb and their cardiovascular performance (heart rate, ejection fraction, fractional shortening, stroke volume, cardiac output, heartbeat variability, and blood flow velocity) was measured and compared. Overall, our finding supports the safety of artificial sweetener exposure. However, several finding like a significant increase in the heart rate and heart rate variability after incubation in several artificial sweeteners are noteworthy. Biomarker testing also revealed that saccharine significantly increase the dopamine level in zebrafish larvae, which is might be the reason for the cardiac physiology changes observed after saccharine exposure.Keywords:artificial sweeteners;zebrafish;cardiac performance
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2018
·
IF 2.88
Hongmei Zhang,Shixin Sun,Yanqing Wang,Zhenghao Fei,Jian Cao
In this work, the interactions between bovine serum albumin (BSA) and five sweeteners including aspartame (APM), acesulfame (AK), sucralose (TGS), sodium cyclamate (SC), and rebaudioside-A (REB-A) have been studied by multispectroscopic techniques, and molecular simulation in order to provide much useful information for the application of new and safer artificial sweeteners. Fluorescence quenching assays indicated that the formation of complexes between sweeteners and BSA mainly induced the fluorescence quenching of protein and the binding site number were about 1 indicting that there is one mainly binding site of APM, AK, TGS, SC, or REB-A in domain of BSA with relatively weak interactions. Molecular modeling results indicated that hydrogen bonding interactions were the mainly binding forces of sweeteners with BSA. Circular dichroism spectra indicated that APM and REB-A obviously induced the secondary structure changes of BSA. The presence of APM increased the fraction of α–Helix of BSA from 65.4% to 73.8%, while the presence of REB-A resulted in decreasing the fraction of α–helix of BSA from 65.4% to 51.2%. The melting temperature studies showed that these five sweeteners except REB-A act as stabilizers to increase the thermal stability of BSA during the thermal denaturation process. In addition, AK, TGS, and SC obviously increased the esterase-like activity of BSA, and such loss of activity of BSA induced by APM and REB-A.
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Food Analytical Methods
·
2018
·Vol. 12
·期 2
·381-393
·
IF 2.245
Sun Xiao-Dong,Wu Hai-Long,Liu Zhi,Chen Yue,Liu Qian,Ding Yu-Jie,Yu Ru-Qin
A rapid and sensitive analytical strategy combining high-performance liquid chromatography-diode array detection (HPLC-DAD) and chemometrics methods was developed for the determination of multi-class food additives in a variety of beverage samples. Different kinds of beverages, which contain diverse unknown interferences, can be directly injected into a chromatographic system after a simple dilution or pretreatment step, and the data were recorded in a short time under a simple gradient elution mode. Although peaks overlapped and changeable interferences existed in the dimension of the chromatographic and spectral in real beverages, all food additives were accurately resolved by using a second-order calibration method based on alternating trilinear decomposition (ATLD) algorithm, and accurate chromatographic profiles, spectral profiles, and concentration profiles were also obtained. Limits of detection for all additives were between 1.40 and 165.1 ng mL −1 , while limits of quantitation ranged from 4.20 to 500.2 ng mL −1 . The spiked recoveries were in the range of 87.3–103% (except amaranth) with RSDs less than 10.2%. Compared with the results obtained by classic HPLC method, our proposed method was more accurate. In all, the proposed method is fast, sensitive, and universal and could be used as a reliable tool to determine food additives and quality monitoring in different complex beverages.
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JOURNAL OF CHROMATOGRAPHY A
·
2016
·
IF 3.926
Yuan-Liang Hu,Zeng-Ping Chen,Yao Chen,Cai-Xia Shi,Ru-Qin Yu
In this contribution, a multiplicative effects model for generalized multiple-internal-standard method (MEM GMIS ) was proposed to solve the signal instability problem of LC–MS over time. MEM GMIS model seamlessly integrates the multiple-internal-standard strategy with multivariate calibration method, and takes full use of all the information carried by multiple internal standards during the quantification of target analytes. Unlike the existing methods based on multiple internal standards, MEM GMIS does not require selecting an optimal internal standard for the quantification of a specific analyte from multiple internal standards used. MEM GMIS was applied to a proof-of-concept model system: the simultaneous quantitative analysis of five edible artificial colorants in two kinds of cocktail drinks. Experimental results demonstrated that MEM GMIS models established on LC–MS data of calibration samples prepared with ultrapure water could provide quite satisfactory concentration predictions for colorants in cocktail samples from their LC–MS data measured 10 days after the LC–MS analysis of the calibration samples. The average relative prediction errors of MEM GMIS models did not exceed 6.0%, considerably better than the corresponding values of commonly used univariate calibration models combined with multiple internal standards. The advantages of good performance and simple implementation render MEM GMIS model a promising alternative tool in quantitative LC–MS assays.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Shixian Chen,Gaohui Du,Yan Cheng,Di Han,Jiahao Deng,Yunting Wang,Huayu Li,Libing Yao,Dong Wang,Qingmei Su,Bingshe Xu
Lithium-sulfur (Li-S) batteries are poised to become the next-generation energy storage technology due to their ultra-high energy density of 2600 Wh kg −1 and the abundant reserves of sulfur on Earth. However, the practical use of Li-S batteries faces several challenges related to sulfur cathodes, including low sulfur loading, significant volumetric expansion, slow conversion kinetics, and the shuttle effect caused by polysulfides. To tackle these challenges, we have developed a hierarchical porous carbon co-doped with nitrogen, oxygen, and sulfur heteroatoms (NOSHPC) that is further loaded with monodispersed vanadium nitride quantum dots (VNQDs). This innovative combination serves as an efficient catalytic matrix to enhance charge transfer processes and facilitate the anchoring and conversion of lithium polysulfides. Moreover, we have fabricated dry-processed cathodes with ultrahigh sulfur loading by using fibrotic polytetrafluoroethylene (PTFE) as a binder, replacing the toxic solvent N-methyl-2-pyrrolidone and polyvinylidene fluoride. The dry-processed VNQDs/NOSHPC/S cathodes demonstrate a remarkable specific capacity of 1655mAh g −1 at 0.1C and achieve exceptional cycling stability, with a decay rate of only 0.0107 % per cycle over 1000 cycles at 1C with a sulfur loading of 5.2 mg cm −2 . Additionally, the pouch cells containing these dry-processed electrodes exhibit a practical areal capacity of 14.2mAh cm −2 (462 Wh kg −1 ) with a sulfur loading of up to 18.6 mg cm −2 and a low electrolyte-to-sulfur ratio of 5.3μL mg −1 . This work presents an innovative strategy for creating sulfur hosts and practical dry-processed Li-S electrodes, highlighting promising prospects for industrial application.
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THERIOGENOLOGY
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2024
·
IF 2.4
Wenjiao Liu,Han wang,Qi Mu,Ting Gong
Taste receptor type 1 subunit 3 (T1R3) is a G protein-coupled receptor encoded by the TAS1R3 gene that can be specifically activated by certain sweeteners or umami agents for sweet/umami recognition. T1R3 is a potential target for regulating male reproduction. However, studies on the impact of non-nutritive sweeteners on reproduction are limited. In the present study, we evaluated the impact of the non-nutritive sweeteners (saccharin sodium, sucralose and acesulfame-K) on testosterone synthesis in testicular Leydig cells of Xiang pigs by comparing the relative abundance of mRNA transcripts and protein expression of T1R3, steroidogenic related factors, and intracellular cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), as well as testosterone levels using Western blotting, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA). To clarify the specific mechanism, a dual luciferase assay was used to uncover the relationship between the transcription factors and steroidogenic enzyme. The acute intratesticular injection of a typical non-nutritive sweeteners was conducted to verify this impact in mouse. The results showed that saccharin sodium not only enhanced T1R3 expression in Leydig cells of Xiang pigs, but also caused significant increases in testosterone, cAMP, PKA, phosphorylation of specificity protein 1 (p-SP1), total protein of specificity protein 1 (SP1), steroidogenic acute regulatory protein (StAR), and 3β-hydroxysteroid dehydrogenase type 1 (3β-HSD1) ( P <0.05). Similarly, treatment of Leydig cells with sucralose and acesulfame-K also increased testosterone level, protein expression of T1R3, 17-α-hydroxylase/17, 20-lyase (CYP17A1), and 3β-HSD1 ( P <0.05). Treatment with SQ22536 (an adenylate cyclas inhibitor) or H89 (a PKA inhibitor) significantly reduced saccharin sodium-induced protein levels of p-SP1, StAR, CYP17A1, and 3β-HSD1 ( P <0.05). In addition, a dual luciferase assay further demonstrated that SP1 significantly increased the promoter activity of CYP17A1 ( P <0.05). When mouse testes were injected with saccharin sodium, T1R3, p-SP1, CYP17A1, and 3β-HSD1 were upregulated, leading to a significant testicular increase in testosterone and cAMP levels ( P <0.05). These results suggest a mechanism by which the taste receptor T1R3 regulates testosterone production, and this mechanism may be linked to the cAMP-PKA pathway. Understanding the interrelationship between T1R3 and the cAMP-PKA-SP1 pathway contributes to clarify the regulatory mechanisms of male reproduction.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Jing Xiao,Dali Zhuo,Jiayuan Tang,Jihong Chen,Chao Tan,Shu Zhang,Shichao Li,Zhirong Zou
The analysis of cyclamate in food samples holds a significant implication for human health, necessitating the development of a selective, sensitive and user-friendly analytical method for analysis of cyclamate. Herein, a pressure meter-based analytical kit was established and applied to selective and sensitive detection of cyclamate. Cyclamate undergoes a selective reaction with NaNO 2 within a hermetically sealed reaction vessel. The reaction produces cyclohexene and N 2 , leading to a discernible pressure change that can be accurately measured using a portable gas pressure meter. Within the concentration range of 20–1000 mg/L, gas pressure values exhibit a proportional relationship with cyclamate concentrations, with a limit of detection of 5.6 mg/L. This analytical kit was further applied to assess cyclamate levels in various beverages and a standard quality control sample (BWQ8791-2016), with recoveries of 93–108 %. It is a simple, easy used, selective and sensitive analytical system for analysis of cyclamate in beverages.
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CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
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2025
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IF 3.8
Zeyu Hou,Bingxin Yan,Yuhan Zhao,Shengbo Zhang,Bo Su,Kai Li,Cunlin Zhang
Artificial sweeteners, as a type of food additive, have vibration frequencies mostly concentrated in the terahertz (THz) band, which enables us to utilize THz technology to deeply analyze their molecular properties. To gain a more comprehensive understanding of the features of artificial sweeteners, this study specifically chose aspartame and acesulfame as representatives. Initially, we employed PXRD and Raman spectroscopy techniques to carry out a thorough examination and verification of the crystalline structure as well as the purity levels of these two synthetic sweeteners. Then, with the aid of Fourier transform infrared spectroscopy (FTIR) technology and terahertz time-domain spectroscopy (THz-TDS) system, the spectral characteristics of aspartame and acesulfame were precisely measured. Moreover, the crystal configurations of these two artificial sweeteners were simulated using solid-state density functional theory (DFT), and the simulation results were in good agreement with the experimental results, further validating the effectiveness of our research methods. Finally, using microfluidic chip technology, the THz spectral characteristics of aspartame and acesulfame in solution were determined, and were compared with their spectra in solid state. We found that the THz spectra of the two artificial sweeteners in solid and solution states have significant correlations. In addition, the research further elucidated that the THz spectrum of a substance dissolved in a solution exhibits a close correlation with its concentration within that solution. These findings provide new perspectives and value for our in-depth research on artificial sweeteners.
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TRIBOLOGY INTERNATIONAL
·
2025
·
IF 6.9
Yingying Yao,Kun Cui,Bingyu Tian,Hongxin He,Ping Wen,Rui Dong,Mingjin Fan
The extensive application of titanium alloy is an important part of machinery and engineering, and the realization of efficient anti-wear performance on the titanium alloy surface is vital to ensure long-term service stability. For the situation that traditional lubricants behave the severe wear on the titanium alloy interface, herein, inspired by the viscosity-lubrication effect of sweeteners, novel ionic liquids (ILs) were designed and synthesized based on sustainable sources. Compared with the control sample poly α-olefin (PAO10) and traditional IL (L-B104), IL lubricants exhibit friction-reducing performance and strong adsorption characteristics. Here, the molecular design strategy combining rigid ring and flexible alkyl chain is the key. The polar groups are preferentially adsorbed on the titanium substrate, and the conjugated system electrons in the rigid ring structure interact with the titanium substrate, as well as the van der Waals force between the cations.
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Environmental Progress & Sustainable Energy
·
2025
·
IF 2.3
Guoting Li,Panpan Yu,Yujie Guo,Xiao Mi,Mingyu Liu,Yunlong Ran,Zhongkun He,Baogui Wang,Yingxu Liu,Tannaz Pak
Secondary effluents are regarded as both sources and sinks of emerging contaminants. Adsorption by activated coke (ACO) is successfully being applied in the advanced treatment of secondary effluent from the wastewater treatment plant of China. However, ACO proved ineffective in removing the sweetener acesulfame (ACE). Herein, both mesoporous ACO and microporous activated carbon (AC) were comparatively used for the innovative confinement of Mn oxides within their porous structures by impregnation/calcination to enhance ACE adsorption. The optimal synthesis conditions were determined to be a MnSO 4 ·H 2 O/ACO mass ratio of 1.0% and a calcination temperature of 600°C (Mn-ACO600). Mn-ACO600 exhibited superior ACE adsorption compared to Mn-modified AC. Stable adsorption performance was observed within the neutral pH range, which favors practical applications. The pseudo-second-order model best described the adsorption kinetics, indicating a possible chemisorption mechanism. Both the Langmuir and Freundlich isotherm models could effectively simulate ACE adsorption, with a q max of 299.6 mg/g at 298 K. Thermodynamic analysis indicated a spontaneous and exothermic process (Δ H 0 = −68.51 kJ/mol) with entropy reduction (Δ S 0 = −218.56 J mol −1 ·K −1 ). Both coexisting inorganic anions and natural organic matter had insignificant influences. Furthermore, the recycled Mn-ACO600 retained an acceptable adsorption capability. Three-dimensional fluorescence excitation-emission matrix analysis demonstrated that Mn-ACO600 adsorption effectively removed organic matter from real secondary effluent.
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危险品化学品经营许可证（带存储）