FOOD SCIENCE AND BIOTECHNOLOGY
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2026
·
IF 3.9
Yu Guofeng,Gao Yuan,Feng Yuan,Qin Yanchong,Li Tianxiao,Xu Chunping,Jia Xuewei,Gao Mingqi
Natural floral aromas are increasingly used in food systems and aroma-functional packaging, but their complex composition and instability limit practical application. This study developed an integrated analytical–computational–sensory strategy to identify key odorants in Chimonanthus praecox flowers for use in food-grade packaging. A total of 38 aroma-active compounds were identified via headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS–SPME–GC–MS). Molecular docking was employed as a screening approach, and binding energy–based clustering enabled the prioritization of odorants with high receptor affinity. Odor activity value (OAV) analysis highlighted nine key odorants, including linalool, ocimene, and methyl salicylate. Molecular docking and dynamics simulations showed π-mediated hydrophobic interactions are critical for odorant recognition and binding stability. Aroma recombination confirmed the importance of these odorants. The reconstructed aroma was successfully applied to food-grade kraft paper, showing high similarity to natural floral aroma. This work promotes the sustainable aroma-functional packaging in food applications.
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JOURNAL OF BIOTECHNOLOGY
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2026
·
IF 3.9
Kefa Hu,Zhipeng Qi,Xiaode Huang,Lei Wang,Xiaomeng Zhang,Shaoheng Tang
Lavandulol and its acetate ester, lavandulyl acetate, are valuable irregular monoterpenes found in lavender essential oil, widely utilized in the food and cosmetic industries. Due to the limitations of extraction from natural plant resources, developing sustainable microbial cell factories for their production is of great interest. Here, we report the first de novo biosynthesis of lavandulol and lavandulyl acetate in Escherichia coli . Initially, a heterologous mevalonate (MVA) pathway was coupled with lavandulyl diphosphate synthase (LPPS) to establish the biosynthetic route from glycerol. To relieve pathway bottlenecks, we overexpressed the endogenous dITP/XTP pyrophosphatase RdgB, which efficiently facilitated the dephosphorylation of the precursor lavandulyl diphosphate (LPP), increasing lavandulol titers to 42.87 mg/L. Subsequently, the alcohol acyltransferase ATF1 from Saccharomyces cerevisiae was identified as the most effective enzyme for acetylating lavandulol. By integrating ATF1 into the pathway and optimizing both the host strain and fermentation process, we achieved a final lavandulyl acetate titer of 89.43 mg/L. This study establishes a promising prokaryotic platform for the efficient biosynthesis of high-value irregular monoterpenes.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
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IF 6.1
Yun-Feng Ma,Lang-Lang Gong,Meng-Qi Zhang,Xuan-Zheng Liu,Huan Guo,J. Joe Hull,Gui-Jun Long,Hong Wang,Youssef Dewer,Fan Zhang,Ming He,Peng He
Insects have evolved an extremely sensitive olfactory system that is essential for a series of physiological and behavioral activities. Some carboxylesterases (CCEs) comprise a major subfamily of odorant-degrading enzymes (ODEs) playing a crucial role in odorant signal inactivation to maintain the odorant receptor sensitivity. In this study, 93 CCEs were annotated in the genome of the German cockroach Blattella germanica, a serious urban pest. Phylogenetic and digital tissue expression pattern analyses identified two antenna-enriched CCEs, BgerCCE021e3 and BgerCCE021d1, as candidate ODEs. RNA interference (RNAi)-mediated knockdown of BgerCCE021e3 and BgerCCE021d1 resulted in partial anosmia with experimental insects exhibiting reduced attraction to ester volatile resources and slower olfactory responses than controls. Furthermore, enzymatic conversion of geranyl acetate by crude male antennal extracts from BgerCCE021e3 and BgerCCE021d1 RNAi insects was also significantly reduced. Our results provide evidence for CCE function in German cockroach olfaction and provide a basis for further exploring behavioral inhibitors that target olfactory-related CCEs.
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Insects
·
2023
·Vol. 14
·期 12
·930
·
IF 3
Jianjun Cheng,Jiawei Gui,Xiaoming Yao,Hong Zhao,Yujie Zhou,Yongjun Du
Simple SummaryThe rice leaf roller,Cnaphalocrocis medinalis,is a migratory insect pest threatening rice production. Monitoring its population dynamics is a critical component of the integrated management system for this pest. Plant odor-trapping has been used as a tool for population monitoring. However, there is a lack of knowledge on the molecular mechanism by which theC. medinalismoth recognizes the plant odor. In the present study, we amplified 12 odor receptor genes and tested differential expression of these genes in male and female moths ofC. medinalis. We identified the function of three olfactory receptor genes inC. medinalis.CmedOR31 was a specific receptor for geraniol, and CmedOR32 was a broad-spectrum OR receptor that responds to both foliar odor and floral scent. The ligands of CmedOR1 were linalool, phenethyl alcohol, 2-phenylethanol, and phenylacetaldehyde. CmedOR1 belonged to a unique conserved OR lineage that strongly responded to phenylacetaldehyde in the evolution ofGlossataspecies, and the OR1 expression inC. medinalisantennae was significantly higher in females than in males. CmedOR1 was the odor receptor forC. medinalisadults to locate food sources.AbstractCnaphalocrocis medinalis(Lepidoptera: Crambidae) is a migratory insect pest on rice crops. The migratoryC. medinalispopulation in a particular location may be immigrants, local populations, emigrants, or a mix of these. Immigrants are strongly attracted to plant odor. We conducted research to identify the olfactory receptors in a floral scent mixture that is strongly attractive toC. medinalis. Through gene cloning, 12 olfactory receptor (OR) genes were amplified and expressed in Xenopus oocytes in vitro, and three of them were found to be responsive to plant foliar and floral volatiles. These were CmedOR31, a specific receptor for geraniol; CmedOR32, a broad-spectrum OR gene that responded to both foliar and floral odors; and CmedOR1, which strongly responded to 10−4M phenylacetaldehyde. The electrophysiological response to phenylacetaldehyde was extremely high, with a current of 3200 ± 86 nA and an extremely high sensitivity. We compared the phylogenetic tree and sequence similarity of CmedOR genes and found that CmedOR1 belonged to a uniquely conserved OR pedigree in the evolution ofGlossataspecies, and the ORs of this pedigree strongly responded to phenylacetaldehyde. The expression of OR1 was significantly higher in the females than in the males. Localization of CmedOR1 in the antennae ofC. medinalisby fluorescence in situ hybridization showed that CmedOR1 was expressed in both males and females. CmedOR1 may be an odor receptor used by females to locate food sources. The function of these ORs and their role in pest monitoring were discussed.Keywords:functional identification;Cnaphalocrocis medinalis;floral odorant;olfactory receptor
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FLAVOUR AND FRAGRANCE JOURNAL
·
2021
·Vol. 37
·期 1
·72-80
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IF 2.576
Yingjie Fu,Yipeng Zhang,Shitong Zeng,Liwen Luo,Hui Xi,Peng Li,Dingzhong Wang,Tougen Liao,Jiajia Chen,Shihao Sun,Jianping Xie
Long-chain alkanes (LA) are common component of rose essential oil (REO). However, the effect of LA on the olfactory characteristics of REO has not been elaborately reported to date. Herein, we utilized three kinds of REO to explore the role of LA in REO. Accordingly, the models of volatiles and LA were established and applied to investigate the effect of LA on flavour release. By addition of LA, the flavour release decreased from 24.26%~101.39% to −15.44%~9.4%, indicating that the proper constitution and proportion of LA were important factors to approach the original behaviour of REO. Moreover, the impact of LA on sensory perception of REO was also revealed by comparing the odour of flavours in the presence and absence of LA. Results showed a significant difference in Kushui samples ( P < .01) and Turkish samples ( P < .05). This work systematically illustrated the functions of LA which regulated the flavour release and the scent of REO, enlightening the further research on REO and rose industry production.
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PEST MANAGEMENT SCIENCE
·
2021
·Vol. 77
·期 6
·2737-2746
·
IF 4.845
Mei-Mei Wang,Gui-Jun Long,Huan Guo,Xuan-Zheng Liu,Hong Wang,Youssef Dewer,Zhao-Qun Li,Kun Liu,Qiu-Liang Zhang,Yun-Feng Ma,Peng He,Ming He
BACKGROUND Carboxyl/cholinesterases (CCEs) are thought to play a pivotal role in the degradation of sex pheromones and plant-derived odorants in insects, but their exact biochemistry and physiological functions remain unclear. RESULTS In this study, two paralogous antennae-enriched CCEs from Plutella xylostella (PxylCCE16a and 16c) were identified and functionally characterized. High-purity protein preparations of active recombinant PxylCCE16a and 16c have been obtained from Sf 9 insect cells by Ni 2+ affinity purification. Our results revealed that the purified recombinant PxylCCE016c is able to degrade two sex pheromone components Z9-14:Ac and Z11-16:Ac at 27.64 ± 0.79% and 24.40 ± 3.07%, respectively, while PxylCCE016a presented relatively lower activity. Additionally, a similar difference in activity was measured in plant-derived odorants. Furthermore, both CCEs displayed obvious preferences for the two sex pheromone components, especially on Z11-16:Ac ( K m values are in the range 7.82–45.06 μmol L –1 ) which much lower than plant odorants ( K m values are in the range 1290–4030 μmol L –1 ). Furthermore, the activity of the two newly identified CCEs is pH-dependent. The activity at pH 6.5 is obviously higher than that at pH 5.0. Interestingly, only PxylCCE016c can be inhibited by a common esterase inhibitor triphenyl phosphate (TPP) with LC 50 of 1570 ± 520 μmol L –1 . CONCLUSION PxylCCE16c plays a more essential role in odorant degradation than PxylCCE16a. Moreover, the current study provides novel potential pesticide targets for the notorious moth Plutella xylostella . © 2021 Society of Chemical Industry
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Qi Lin,Cheng Peng,Kunpeng Yu,Yanling Lin,Yongquan Xu,Lijun Li,Hui Ni,Feng Chen
The β-glucosidases known to improve tea aroma are all mesothermal enzymes, limiting their use under brewing conditions. Based on the properties analysis and molecular docking, the thermostable β-glucosidase (TPG) from Thermotoga petrophlia showed potential to enhance tea aroma. Treatment by recombinant TPG at 90 °C, the floral, sweet and grassy notes of instant Oolong tea were increased, while the roasted, caramel and woody notes were decreased. The improved floral, sweet and grassy notes were related to increase releasing of benzyl alcohol (floral), geraniol (floral), (Z)-3-hexen-1-ol (grassy), benzaldehyde (sweet) and 1-hexanol (grassy) by TPG hydrolyzing of (Z)-3-hexenyl-β-D-glucopyranoside, hexanyl-β-D-glucopyranoside (HGP), benzyl-β-D-glucopyranoside, prunasin and geranyl-β-D-glucopyranoside (GGP), respectively. Although the catalytic efficiency of TGP to GGP was about twice that to HGP, HPG was more competitive than GGP when they mixed. Combined with microstructure analysis, the structure-function relationship of TPG-influencing tea aroma were understood. This study provided the method of how to mining new function of characterized β-glucosidases, as well as a theoretical basis for the development of new tea products.
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Plants-Basel
·
2024
·Vol. 13
·期 20
·2946
·
IF 4
Jiuying Li,Yinghui Zhu,Lanlan Sun,Hongle Xu,Wangcang Su,Fei Xue,Chuantao Lu,Wenwei Tang,Renhai Wu
Fenoxaprop-P-ethyl (FE) can effectively control weeds in rice fields, but it has been found to cause phytotoxicity in rice. In this study, the phytotoxicity of FE was mitigated by perilla leaf essential oil (PEO) in rice seedlings. The injury recovery rates (IRRs) for shoot length and fresh weight treated with 800 mg/L of PEO were 101.51% and 99.05%, respectively. Moreover, the damage ofs-metolachlor and pretilachlor was also alleviated when co-applied with 800 mg/L PEO; the IRR ofs-metolachlor phytotoxicity was 26.07% and 27.34%, respectively, and the IRR of pretilachlor phytotoxicity was 127.27% and 124.39%, respectively. However, PEO had no significant effect on the phytotoxicity of pinoxaden, mesotrione, penoxsulam, mesosulfuron-methyl, and nicosulfuron. The results of GC–MS analysis showed that a total of 23 components were detected in PEO, among which linalool (36.49%), linalyl formate (26.96%), α-terpineol (10.63%), 2-hexanoylfuran (5.81%), geranyl acetate (4.13%), and neryl acetate (2.30%) were the primary components. Among them, 2-hexanoylfuran was the most effective component to alleviate FE damage, for which the IRR of shoot length and fresh weight was 73.17% and 73.02%, respectively, followed by the geranyl acetate, for which the IRR was 72.32% and 60.56%, respectively, and neryl acetate, for which the IRR was 65.28% and 58.11%, respectively. Furthermore, the application of 50 mg/L of 2-hexanofuran significantly improved the tolerance of shoot length and fresh weight to FE stress by factors of 5.32 and 5.35, respectively. This research demonstrates that PEO and 2-hexanoylfuran have the potential to serve as natural safeners to reduce phytotoxicity.
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