JOURNAL OF FOOD QUALITY
·
2022
·
IF 3.2
Yingqi Chen,Chonggao Hu,Jiaojiao Xu,Xiaodong Pan,Baifen Huang
In this paper, we built a method of verifying the 4 retinols of vitamin A and 8 tocopherol isomers of vitamin E in the food industry based on RP-HPLC-PDA-FLR. The effect of laboratory light conditions on the target components was considered for the first time, and it was found that the loss rate of the target components was the smallest in the case of a white laboratory bench with red or LED light in the dark room. There was no finding of extraction recoveries of the target components with a significant difference under different saponification conditions. Meanwhile, it was found that using ethyl acetate/n-hexane mixed solvent as the extraction solvent could ensure the effective extraction of the target components. Finally, baseline separation of 12 components was achieved within 45 min using the C30 column. With the help of methodological verification, we found that the recovery rate ranged from 76.45% to 93.52%, and RSD was between 0.19% and 12.99%; the Limit of Detection minimum value was 0.01 mg/100 g and the Limit of Quantitation minimum value was 0.03 mg/100 g. The detection method was successfully applied to the distribution detection of 4 kinds of retinols and 8 kinds of vitamin E in egg and dairy products and provided technical support for the accurate nutritional evaluation of vitamin A and vitamin E.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Luyao Fan,Hongchao Dai,Wenyuan Zhou,Lei Yuan,Jia Yang,Zhenquan Yang,Xin-an Jiao
Bacillus licheniformis , a quick and strong biofilm former, is served as a persistent microbial contamination in the dairy industry. Its biofilm formation process is usually regulated by environmental factors including the divalent cation Ca 2+ . This work aims to investigate how different concentrations of Ca 2+ change biofilm-related phenotypes (bacterial motility, biofilm-forming capacity, biofilm structures, and EPS production) of dairy B . licheniformis strains. The Ca 2+ ions dependent regulation mechanism for B . licheniformis biofilm formation was further investigated by RNA-sequencing analysis. Results revealed that supplementation of Ca 2+ increased B . licheniformis biofilm formation in a dose-dependent way, and enhanced average coverage and thickness of biofilms with complex structures were observed by confocal laser scanning microscopy. Bacterial mobility of B. licheniformis was increased by the supplementation of Ca 2+ except the swarming ability at 20 mM of Ca 2+ . The addition of Ca 2+ decreased the contents of polysaccharides but promoted proteins production in EPS, and the ratio of proteins/polysaccharides content was significantly enhanced with increasing Ca 2+ concentrations. RNA-sequencing results clearly indicated the variation in regulating biofilm formation under different Ca 2+ concentrations, as 939 (671 upregulated and 268 downregulated) and 951 genes (581 upregulated and 370 downregulated) in B. licheniformis BL2-11 were induced by 10 and 20 mM of Ca 2+ , respectively. Differential genes were annotated in various KEGG pathways, including flagellar assembly, two-component system, quorum sensing, ABC transporters, and related carbohydrate and amino acid metabolism pathways. Collectively, the results unravel the significance of Ca 2+ as a biofilm-promoting signal for B. licheniformis in the dairy industry.
研究领域:
微生物学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CyTA-Journal of Food
·
2025
·
IF 1.7
Zhou Lu,Zhao Junpeng,Hilary Kwesi Ketemepi
A QuEChERS-UPLC-MS/MS method was developed and validated for simultaneous determination of six pesticides (chlorpyrifos, malathion, carbofuran, thiodicarb, deltamethrin, and lambda-cyhalothrin) in dairy products. Samples (n=50), collected from a supermarket in Chongqing, China, were extracted with 1% acetic acid–acetonitrile, purified using PSA, and quantified by external-standard UPLC-MS/MS. All pesticides showed excellent linearity (0.001–0.50 mg/L, R² > 0.99), with LOQs of 0.002 mg/kg. Average recoveries at four spiking levels (0.002, 0.01, 0.1, 1.0 mg/kg) ranged from 89% to 110% (RSD 0.5–9.5%, n=5). Analysis of real samples revealed residues below China’s MRLs (GB 2763–2021), confirming regulatory compliance. The method meets analytical requirements for dairy matrices and offers a reliable framework adaptable to other international standards.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
·
2020
·Vol. 31
·期 6
·1216-1224
·
IF 1.399
Han Qing,Wei Lanlan,Deng Guozhe,Sun Ying,Deng Jianjun,Tang Zigang,Fan Jinhui,Wang Xia,Kang Xuejun
The detection of vitamin D in complex samples is challenging because of the instability of vitamin D in sample preparation. To resolve this problem, packed-fiber solid-phase extraction (PFSPE) has been successfully developed for the pre-treatment of samples containing vitamin D2 (D2) and vitamin D3 (D3) in dairy products. Based on PFSPE, vitamin D could avoid oxidation because of the shorter extraction time. The adsorbent materials, polystyrene/polypyrrole (PS/PPy) nanofibers, were prepared by electrospinning of PS and in situ polymerization of PPy on the surface of PS nanofibers. The linearity was within the range of 1-2000 ng mL−1 in dairy solution, and the recoveries were 91.9-109.1% with a relative standard deviation (RSD) < 13.6%. Therefore, the prepared method could be used for the detection of D2 and D3 in dairy products.
研究领域:
分析化学
·
食品科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2015
·
IF 3.391
Wei-jun Zhao,Yonghuan Wang,Juan Li,Ling-feng Li,Qi Wang,Ke Han,Yuang Zhang,Xin Li,Peng Li,Jikui Luo,Xiaozhi Wang
This article presents a method for sensitive, fast and quantitative determination of melamine in milk and dairy products using high-field asymmetric ion mobility spectrometry (FAIMS). The solid-phase extraction (SPE) technology was used for purification after the sample was extracted by organic solvents, and followed by the analysis of FAIMS . The measurement parameters and variables that affect the FAIMS detection have been investigated, and optimum conditions have been obtained as follows: the carrier gas flow rate is 1.6 L min −1 , the headspace sampler temperature is 150 °C, the pressure is 1 atm, and the humidity is 2.0 g m −3 . The results showed that the SPE–FAIMS method can detect melamine in samples with a concentration down to 0.1 mg kg −1 . The ion intensity has a linear relationship with melamine concentration in the range from 0.3 mg L −1 to 25 mg L −1 , with a good linearity of 0.9975. The limits of detection (LOD) and limits of quantification (LOQ) are 0.1 mg kg −1 and 0.3 mg kg −1 in milk and dairy products, respectively, and the relative standard deviation is less than 8.0%. The results demonstrated that FAIMS has great potential as a powerful tool for food analysis and safety inspection.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ruiqi Gao,Simin Ye,Xiaofang Yang,Ying Zhang,Yuanyuan Liu,Jinyi Zhang,Chengbin Zheng
Accurately detecting iodine in dairy products is paramount to ensure adequate intake and prevent iodine excess or deficiency-related diseases. However, the development of rapid sample digestion-coupled portable sensitive assays represents a significant challenge in the home-based analysis of iodine content in dairy products. Herein, an integrated portable device was developed, which combines sample digestion, microextraction, and fluorescence signal reading. The system ensures highly sensitive detection with minimal matrix interference by utilizing a rapid thermal-assisted digestion technique with minimal sample volume (500 μL) and high-enrichment headspace single-drop microextraction. Under optimal conditions, the ratiometric fluorescence filter effect system, coupled with a smartphone, gives a detection limit of 7 nM for iodine. Moreover, the accuracy of the strategy is further validated by real sample analyses and standard method comparisons. This method provides a practical tool for monitoring dietary iodine intake, particularly infant nutrition.
研究领域:
分析化学
·
营养学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Science and Human Wellness
·
2024
·
IF 5.6
Hyemin Oh,Yohan Yoon
This study investigated if the variation in the effect of anti-cholesterol (AC) treatment on individual mice are related to gut microbiome composition. The bile salt hydrolase (BSH) activity of 23 commercial fermented milk products was examined to select a fermented milk product for AC treatment. Mice were fed to different diets for 6 weeks: high-fat (660 % of total calories from fat; D1), high-dietary fibre (20 % cellulose; D2), and low-fat (17.2 % of total calories from fat; D3) diets to change their gut microbiomes. Subsequently, faecal microbiome was transplanted (FMT) into mice treated with high cholesterol diet contained 2 % cholesterol, followed by AC or non-AC (sterile tap water, STW) treatments. Control groups with normal (NC) and high-cholesterol diets (PC) were prepared for both AC and STW treatment. All experimental groups were subjected to serum and liver cholesterol, cholesterol metabolism-related (CMR) gene expression, and intestinal microbiome analyses. D3-FMT mice showed the most significant enhancements in cholesterol ratio and decreased hepatic cholesterol levels with AC treatment. Moreover, upregulation of the Cyp7a1 gene expression was observed in this group. Furthermore, the intestinal microbiome analysis indicated higher abundances of BSH-producing Eubacterium , Bifidobacterium , and Parabacteroides in the D3-FMT + AC group compare to others, potentially contributing to increased bile acid synthesis.
研究领域:
医学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaoyun Lei,Yaping Jiang,Yang Li,Yanping Duan,Ting Guo,Tianlei Zhang,Zuoping Zhao,Huiping Dai,Xiaohui Ji
The pretreatment of complex food matrices is challenging due to matrix interferences and non-specific adsorption of macromolecules. This study proposes a biocompatible solid-phase microextraction method to effectively enrich polypeptide antibiotics in dairy products using various zwitterionic-based capillary microextraction (CME) techniques. The proposed method demonstrated a broad linear range of 20–800 μg L −1 , with a detection limit (LOD) of 10 μg L −1 when employing the phosphorylcholine-based column for polypeptide antibiotics extraction. Additionally, a linear range of 8.0–1000 μg L −1 was achieved, with a lower detection limit of 5.4 μg L −1 on the sulfobetaine-based column. Density functional theory (DFT) calculations indicated that zwitterionic CME columns containing typical phosphorylcholine and sulfobetaine groups facilitate target capture primarily through hydrophilic, electrostatic, and hydrogen bonding interactions. This advanced method was applied to detect peptide residues in a variety of dairy products, yielding results that significantly improved detection accuracy and demonstrated potential practical application value.
研究领域:
分析化学
·
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Hongjie Dai,Yingjie Liu,Xi Zheng,Xiyue Hu,Liang Ma,Hongxia Wang,Hai Chen,Yuhao Zhang
Non-dairy whipped creams (NDWC) are a typical food emulsion system and are gaining popularity among consumers. Oleogels as reasonable alternatives to trans and saturated fats in foods show great potential application in NDWC. Effects of different proportions of oleogel (30 %–70 %) as base oil on the crystallization behavior, appearance, interface and rheological properties of NDWC were evaluated. The base oil made of oleogel and sunflower oil can crystallize at 0–10 °C, showing needle-liked β-crystal crystal structure. A higher oleogel proportion increased solid fat index, fat crystals and fractal dimension. The fat coalescence rate in NDWC gradually increased from 205.88 % to 465.96 % as oleogel ratio increased from 30 % to 70 %, which was beneficial to the network structure formation of NDWC. The increase of oleogel ratio effectively reduced interfacial tension and increased the elastic modulus as well as promoted partial fat coalescence, thus facilitated the formation and stabilization of the NDWC system.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2014
·
IF 3.511
Xinai Zhang,Fan Zhang,Hongyin Zhang,Jianzhong Shen,En Han,Xiaoya Dong
In this paper, we report an amplified electrochemical immunoassay for Escherichia coli as indicator bacteria relevant to the quality of dairy product using the functionalized gold nanorod-based labels ({ d Ab–AuNR–FCA}). The { d Ab–AuNR–FCA} labels were designed by exploiting silica-functionalized gold nanorods (AuNR@SiO 2 ) as the carriers for immobilization of detection antibody ( d Ab) and ferrocenecarboxylic acid (FCA), in which d Ab was used for recognition of E. coli and FCA tags served as signal-generating molecule. Greatly amplified signal was achieved in the sandwich-type immunoassay when enormous FCA linked to AuNR@SiO 2 . Compared with the commercially available { d Ab–FCA}, the { d Ab–AuNR–FCA} labels exhibited a better performance for E. coli assay due to the advantages of AuNR@SiO 2 as carriers. Under optimal experimental conditions, it showed a linear relationship between the peak current of FCA and the logarithmic value of E. coli concentration ranging from 1.0×10 2 to 5.0×10 4 cfu mL −1 with a detection limit of 60 cfu mL −1 ( S / N =3), and the electrochemical detection of E. coli could be achieved in 3 h. Moreover, the proposed strategy was used to determine E. coli in dairy product (pure fresh milk, yogurt in shelf-life, and expired yogurt), and the recoveries of standard additions were in the range of 95.1–106%. This proposed strategy exhibited rapid response, high sensitivity and specificity for E. coli assay in dairy product, and could become a promising technique to estimate the quality of dairy product.
研究领域:
分析化学
·
食品安全
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 61
·34877-34882
·
IF 2.936
Xue Han,Zhixin Qin,Mengyao Zhao,Jiajia Song,Fei Qu,Fengli Qu,Rong-Mei Kong
The illegal adulteration of melamine in dairy products for false protein content increase is a strong hazard to human health. Herein, a simple and sensitive colorimetric method was developed for the quantification of melamine in dairy products based on a Cu2+-hydrogen peroxide (H2O2)-3,3′,5,5′-tetramethylbenzidine (TMB) system. In this strategy, Cu2+ exhibits peroxidase-like activity and can catalyze the oxidation of TMB to oxidized TMB (oxTMB) in the presence of H2O2 with a blue colour change of the solution. However, the presence of melamine quickly interacts with H2O2 leading to the consumption of H2O2 and thus strongly hinders the oxidation of TMB. Under the optimal conditions, the absorbance change of oxTMB has a linear response to the concentration of melamine from 1 to 100 μM with a detection limit of 0.5 μM for melamine. The proposed method has many merits including more simplicity, good selectivity, and more cost-effectiveness without using any nanomaterials. The method was further successfully applied to detect melamine in dairy products including milk and infant formula powder.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Hao Luo,Yihong Bao,Ping Zhu
Functional lycopene-rich yogurt displays attractive nutritious and health-promoting benefits among existing functional dairy products, owing to supplement with lycopene which could enhance immunity, prevent cancer, and cardiovascular diseases. Due to poor stability and fat-solubility of lycopene, its incorporation into yogurt is challengeable. In this study, carotenoid genes for lycopene synthesis were co-introduced into probiotic Bacillus subtilis for efficient lycopene production. Further engineered B. subtilis was applied as adjunct starter culture for achieving lycopene-rich yogurt. Developed yogurt exhibited desirable physiochemical characteristics compared with plain yogurt. Moreover, lycopene-rich yogurt was endowed with significantly high antioxidant capacity. More importantly, this functionalized yogurt had attractive sensorial attributes for quality-assured food to facilitate consumer acceptance. As the first report of fortifying yogurt of lycopene using B. subtilis with improved functional properties, this study offers a new and facile clue to enrich bioactive lycopene and probiotic B. subtilis in yogurt for healthy and nutritional food development.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）