ANALYST
·
2019
·Vol. 145
·期 3
·1025-1032
·
IF 4.019
Hui Xu,Zijie Feng,Yingxiang Du
Recently, ionic liquids have been widely used for chiral separation. However, several papers have reported the use of ionic liquids as the sole chiral selector. In this work, a novel capillary electrophoresis method using the ionic liquid 3-methyl-1-(3-sulfopropyl)-1H-imidazol-3-ium lactobionate (MSI-LA) as a chiral selector was developed for the enantioseparation of propranolol, metoprolol, bisoprolol, esmolol, atenolol, sotalol, terbutaline and clenbuterol. Method optimization was conducted and the optimized method (40 mM borax buffer, v/v 30% methanol, pH 8.5, 160 mM MSI-LA; +10 kV voltage; capillary temperature, 15 °C; 50 mbar/5 s injection; detection wavelength, 230 nm) was successful for the baseline separation of the eight basic drugs. The LA (lactobionic acid) system was compared. The possible mechanisms of enantioseparation were investigated using molecular modeling. Both the cation and anion of MSI-LA provided interactions between the chiral selector and analytes, and finally enhanced chiral recognition capability compared with LA.
研究领域:
分析化学
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药物分析
·
分子生物学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2023
·
IF 5.8
Shasha Qin,Jiamin Li,Zhiling Pan,Can Wang,Bing-Feng Zhang
Prodrug nanoassemblies fabricated by anticancer drug conjugates exhibited more advantages in controlled drug release and bioavailability and favorable antitumor efficacy. In this paper, lactobionic acid (LA) was connected with polyethylene glycol through amido linkages, and paclitaxel was joined with polyethylene glycol by means of ester bonds to form the prodrug copolymer LA-PEG-PTX. Then, LA-PEG-PTX was automatically assembled into LA-PEG-PTX nanoparticles (LPP NPs) by dialysis. The LPP NPs had a relatively uniform size of approximately 200 nm, a negative potential (−13.68 mV), and a spherical shape under TEM. The drug loading of LPP NPs was 3.91%, which was measured by HPLC. The in vitro release profile of LPP NPs exhibited a sustained release feature. The results of the pharmacokinetic test in rats showed that LPP NPs had higher T 1/2 and AUC values than the control group (free PTX) and a prolonged in vivo circulation time, thus increasing the bioavailability of PTX. Remarkably, the LPP NPs were absorbed into HepG2 cells after galactose-directed internalization and enhanced cytotoxicity. Consequently, LPP NPs displayed notable antitumor activity in Kunming mice with H22 hepatocellular carcinoma. Collectively, these findings suggested that paclitaxel prodrug-based self-assembled nanoparticles were a promising alternative for improving the bioavailability and antitumor effect of PTX.
研究领域:
药剂学
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肿瘤学
·
生物医学工程
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DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Ruyun Lou,Weiting Yu,Yizhe Song,Ying Ren,Huizhen Zheng,Xin Guo,Yunfei Lin,Guoyu Pan,Xiuli Wang,Xiaojun Ma
Galactose moieties are covalently coupled with sodium alginate to enhance liver-specific functions in microcapsules owing to the specific interaction between the galactose moieties and the asialoglycoprotein receptors (ASGPRs) of hepatocytes. In this study, galactosylated alginate (L-NH 2 -OH-alginate) based microcapsules with desirable stability and a suitable 3D microenvironment are designed and fabricated for primary hepatocyte applications. The designed L-NH 2 -OH-alginate is fabricated via the application of ethylenediamine grafted lactobionic acid (L-NH 2 ) onto the hydroxyl groups of sodium alginate so that the negatively charged carboxyl groups intact in L-NH 2 -OH-alginate can effectively bond with Ca 2+ to form a stable three-dimensional gel network; a subsequent reaction with polycations forms a stable membrane of microcapsules. As a result, L-NH 2 -OH-alginate based microcapsules exhibit an excellent mechanical stability. Moreover, with a higher degree of substitution in L-NH 2 -OH-alginate (DS 0.41), the hepatocytes entrapped in L-NH 2 -OH-alginate microcapsules exhibit better viability and well-maintained liver-specific functions.
研究领域:
生物材料学
·
细胞工程
·
肝脏研究
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 12
·1-8
·
IF 6.232
Ding Wen,Ma Mingxuan,Du Yingxiang,Chen Cheng,Ma Xiaofei
An in situ zeolite imidazole metal organic framework-90 (ZIF-90) modified capillary was prepared via the method of solvothermal synthesis. The coating of ZIF-90 was characterized by scanning electron microscopy, energy-dispersive X-ray spectrometry, and EOF. Capillary electrochromatography–based enantioseparation of the basic drugs propranolol (PRO), metoprolol (MET), atenolol (ATE), bisoprolol (BIS), and sotalol (SOT) was performed using lactobionic acid (LA) as the chiral selector. Compared with an uncoated silica capillary, the resolutions are greatly improved (PRO 1.40 → 3.23; MET 1.07 → 3.19; ATE 1.07 → 3.15; BIS 1.16 → 3.41; SOT 1.00 → 2.79). Effects of buffer pH values, proportion of organic additives, concentration of lactobionic acid, and applied voltage were investigated.
Schematic presentation of the preparation of zeolitic imidazolate framework-90 (ZIF-90) modified capillary (ZIF-90@capillary) for enantioseparation of drug enantiomers. The capillary was applied to construct capillary electrochromatography system with lactobionic acid for enantioseparation of basic chiral drugs.
研究领域:
分析化学
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药物分析
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Jie Liu,Yingxiang Du,Xiaodong Sun,Zijie Feng,Xiaofei Ma,Jingtang Li
In this paper, a chiral selector, N-(4-H-1, 2, 4-triazolium)-lactobionamides (LA-ATM), was synthesized and applied for enantioseparation in capillary electrophoresis (CE) for the first time. Compared with lactobionic acid (LA) separation system, enhanced enantioseparation of five tested drugs was achieved in this modification system. In order to achieve good chiral separation, several parameters such as chiral selector concentration, buffer pH, applied voltage as well as the type and proportion of organic modifier were systematically investigated. Molecular modeling was applied to demonstrate the chiral recognition mechanism of the LA-ATM, which well supported the experimental results. Furthermore, a mathematical equation built up based on the molecular mechanics calculations was used in predicting resolution of tested drugs using LA or LA-ATM mediated CE, the predicted result was well correlated with the experimental result.
研究领域:
分析化学
·
药物化学
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DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Panxianzhi Ni,Renpeng Li,Sheng Ye,Jing Shan,Tun Yuan,Jie Liang,Yujiang Fan,Xingdong Zhang
To develop a biomaterial to lift the lesion and promote wound healing in endoscopic submucosal dissection (ESD), we used lactobionic acid (LA) to improve the water solubility of chitosan (CS) and prepared a new three-phase hydrogel system with lactobionic acid-modified chitosan/chitosan/β-glycerophosphate (CSLA/CS/GP). The results indicated that the hydrogel retains temperature-sensitive properties, and CSLA obviously improved the low-temperature fluidity of the hydrogel precursor solution, enabling injection of the hydrogel by endoscopic needle. The mechanical strength and bio-adhesion of the hydrogels were also improved by the addition of CSLA and the hydrogels could be maintained in acidic environment for a few days and exhibit greater protection of cells. The CSLA/CS/GP hydrogels show good cytocompatibility. The heights of cushions elevated by CSLA/CS/GP hydrogels remained ∼ 60 % 2 h post-injection in porcine stomach models. Given the unique characteristics of these materials, the CSLA/CS/GP thermo-sensitive hydrogel is a promising intraoperative biomaterial in ESD.
研究领域:
生物材料学
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医学工程
·
消化病学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Dao-Qiang Lu,Dahai Liu,Justin Liu,Wen-Xing Li,Yilong Ai,Jun Wang,Daogang Guan
Chitosan-based nanogels are effective carriers for drug delivery due to their biocompatibility and biodegradability. However, the chemically cross-linked nanogels usually require complicated procedures or tough conditions. Herein, we report a simple approach to generate chitosan-based nanogels by photo-crosslinking of poor solvent-induced nanoaggregates without requiring any emulsifying agent, catalyst, or external crosslinker. O-nitrobenzyl alcohol-modified carboxymethyl chitosan was synthesized and self-crosslinked into the nanogels in a mixed solution of ethanol and water under 365 nm light irradiation due to UV-induced primary amine and o-nitrobenzyl alcohol cyclization. The nanogels (CMC-NBA NPs) and lactobionic acid-decorated nanogels (LACMC-NBA NPs) displayed a uniform diameter (~200 nm) and excellent stability under physiological conditions. Notably, the nanogels exhibited a high loading content (~28 %) due to π-π stacking and electrostatic interactions between doxorubicin (DOX) and the carriers. These DOX-loaded nanogels showed rapid drug release under slightly acidic conditions. The cell and animal experiments confirmed that LACMC-NBA NPs increased cellular uptake, improved cytotoxicity in tumor cells, and enhanced growth inhibition in vivo than CMC-NBA NPs. Thus, these photo-crosslinked nanogels possess great potential for DOX delivery.
研究领域:
药学
·
纳米技术
·
药物递送系统
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2019
·
IF 6.361
Chunzhi Huang,Xu Hu,Zhaosheng Hou,Jianbo Ji,Zhonghao Li,Yuxia Luan
Graphene oxide (GO), as a drug delivery carrier, has attracted considerable attention because of its interesting properties. However, GO tends to aggregate in aqueous solution. Amphiphilic molecules are usually necessary to stabilize GO. The introduction of these non-functional macromolecules on the one hand reduces drug loading, but on the other hand may cause unpredictable side effects. This study proposes a new strategy for stabilizing GO with a functional photothermal agent, IR820 (new indocyanine green) derivative. IR820 derivative results from the conjugation of active targeted lactobionic acid (LA) with IR820 for the formation of IR820-LA. IR820-LA features central aromatic groups that can associate with the GO basal plane through π-π interactions. The flanking moiety of hydrophilic LA and sulfonic groups thus provides steric stabilization of GO in aqueous solution. Moreover, IR820-LA endows GO/doxorubicin (GO/DOX) nanovehicles with fluorescence imaging ability and actively targeted chemo-photothermal therapy. Experimental results both in vitro and in vivo have indicated its good chemo-photothermal therapeutic effect according to its active tumor targeting ability and pH-sensitive drug release characteristics. Therefore, our GO/DOX/IR820-LA nanohybrids can be excellent nanoplatforms for active tumor-targeted chemo-photothermal therapy with imaging guidance.
研究领域:
生物医学工程
·
纳米医学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Ranran Zhao,Hongxin Liu,Zhigang Xie,Min Zheng
Nanovaccines possess tremendous potential in efficient antigen delivery and the induction of robust tumor-specific immune responses. Nevertheless, the development of nanovaccines with enhanced efficacy and improved safety is an ongoing quest. In this research, chiral carbon dots (L/D-CDs) were modified with lactobionic acid (LA) to fabricate LLA/DLA. Subsequently, L/D-CDs and LLA/DLA were combined with the model antigen ovalbumin (OVA) to formulate nanovaccines (L-OVA, D-OVA, LLAO, and DLAO). Interestingly, D-type nanovaccines (D-OVA and DLAO) exhibited higher antigen uptake by dendritic cells (DCs) and more potent immune activities in contrast to their corresponding L-type nanovaccines (L-OVA and LLAO), suggesting a chiral-dependent augmentation in antigen presentation and immunogenicity. Moreover, LA-modified nanovaccines (LLAO and DLAO) showed enhanced antigen processing and presentation, along with markedly elevated expression of CD80 and CD86. Immunization with DLAO in tumor-bearing mice induced the most significant antigen-specific T cell proliferation in the spleen, lymph nodes (LNs), and tumor sites, which further translated into the most remarkable therapeutic effects. Taken together, these results underscore the considerable promise of leveraging chirality and targeting to potentiate anti-tumor immunity.
研究领域:
肿瘤学
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免疫学
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纳米医学
·
转化医学
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分子影像
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF POWER SOURCES
·
2024
·
IF 8.1
Wenping Zeng,Xudong Wang,Yun Huang,Yunhe Zhang,He Zheng,Jie Xiao,Lei Fu,Xichang Wang,Xing Li,Mingshan Wang,Bingshu Guo,Yuanhua Lin
The growth of dendritic dendrites and side reactions at the zinc anode result in poor cycling stability of the aqueous zinc-ion battery (AZIBs). Here, we report on a multifunctional additive, Sodium lactobionate (SL), that achieves more stable cycling performance. SL molecule can loosen the solvation structure of water and Zn 2+ , inhibit corrosion and hydrogen evolution reactions , and regulate ion deposition in AZIBs. Under the action of SL, the Zn//Zn symmetric battery achieves an ultra-long stable cycle of up to 2150 h at a current density of 2 mA·cm −2 and an area capacity of 1 mAh cm −2 and Zn//NVO can still perform 1110 cycles at the current density of 2 A g −1 and maintain the reversible specific discharge capacity of 179 mAh g −1 . In addition, at 2 mA cm −2 and 0.5 mAh cm −2 , the Zn//Cu asymmetric battery achieved up to 350 cycles with a coulomb efficiency of 99.5 %.
研究领域:
材料科学
·
电化学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Shuli Man,Wei Liu,Jingxian Bi,Jingjing Bai,Qiong Wu,Bin Hu,Jing Hu,Long Ma
Curcumin (CUR) as one of the natural edible pigments is approved by the World Health Organization due to its nontoxic and anticancer effect. However, the utility of CUR is restricted due to its low oral bioavailability. Nanoparticle drug delivery systems like mesoporous silica nanoparticles (MSNs) have been extensively used due to their high specific surface area, high loading rate, and ease of modification. This study developed lactobionic acid (LA)-modified carboxymethyl chitosan (CMCS)-coated MSNs to deliver CUR specifically targeting hepatocellular carcinoma. Among these nanoparticles, LA targets liver cancer cells. CMCS utilizes pH-responsive release of CUR. The LA-CMCS-MSN@CUR (MSN@CUR) were evaluated using several methods, including Fourier transform infrared spectroscopy, transmission electron microscopy, and zeta potential measurements. Liver cellular uptake of MSN@CUR depends on a specific LA receptor-mediated endocytosis mechanism. Additionally, MSN@CUR performed with a better antitumor effect than Cur in H22 orthotopic transplantation of liver cancer and H22 solid tumor mouse models. Treatment with MSN@CUR significantly reduced the protein of VEGF, p-PI3K, and AKT, increased the protein of caspases 3 and 8, ultimately inhibited tumor migration, and promoted apoptosis. This study provides a new path for delivery of natural active ingredients with excellent bioavailability in the antitumor field.
研究领域:
药剂学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE RESEARCH
·
2021
·
IF 2.104
Jun Wang,Zongyong Zhang,Yilong Ai,Fang Liu,Min-Min Chen,Dahai Liu
In order to achieve efficient delivery of methotrexate (MTX), thymine-chitosan nanoparticles (Thy-Cs NPs) were prepared, and further decorated with lactobionic acid (LA) to obtain tumor-targeting nanoparticles (LA-Thy-Cs NPs). These nanoparticles possessed a regular spherical structure with the average size about 190–250 nm and narrow size distribution, which were kinetically stable in the physiological environment. Due to electrostatic interactions and multiple hydrogen-bonding interactions between MTX and carriers, MTX was loaded into Thy-Cs NPs with high drug loading content (~20%). MTX release from Thy-Cs NPs was significantly accelerated in the mildly acidic environment due to the destruction of two types of non-covalent interactions. In vitro cell experiments demonstrated that LA-Thy-Cs NPs could be efficiently internalized into hepatoma carcinoma cells, leading to higher cytotoxicity. Moreover, MTX-loaded LA-Thy-Cs NPs performed an enhanced growth inhibition in three-dimensional multicellular tumor spheroids . Thus, the LA decorated thymine-chitosan nanocarriers can be a promising candidate for efficient delivery of MTX.
研究领域:
药学
·
纳米技术
·
癌症研究
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）