Journal of Food Measurement and Characterization
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2017
·Vol. 11
·期 4
·1959-1968
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IF 0.536
Arslan Muhammad,Rakha Allah,Khan Moazzam Rafiq,Zou Xiaobo
Celiac disease (CD) is a genetic autoimmune disorder of the small intestine, occurring in genetically predisposed people with a prevalence of about 1% in global population. To combat the CD, lifelong adherence to gluten free (GF) diet is required. The GF breads are generally deficient in nutrients such as dietary fiber, antioxidants, vitamins, minerals etc. To overcome this deficiency, GF bread was supplemented with guava pulp powder (GPP) at 5 different level (0, 2.5, 5.0, 7.5 and 10%). Guava ( Psidium guajava ) contains dietary fiber and total phenolics content, 12.72% and 927.66 mg gallic acid equivalent (GAE)/100 g, respectively. Results showed that, the addition of GPP significantly (p < 0.05) increased the crude fiber and total phenolic content of GF bread from 0.92 to 2.45% and 14.46 to 103.77 mg GAE/100 g respectively. The loaf volume increased up to a level of 492.00 cm 3 , hence decreased the hardness from 2.58 to 2.38 N. Furthermore, as the concentration of GPP increased from (0–10%) the L* value of GF bread decreased. The addition of GPP, non-significantly (p > 0.05) affected the protein content and calorific value of GF bread. Sensorial analysis showed that, GF bread supplemented with 5% of GPP presented overall acceptability better when compared with control.
研究领域:
食品科学
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营养学
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医学
✓ 已验证
产品关联
DOI
高影响因子
Food Science & Nutrition
·
2025
·Vol. 13
·期 10
·e71000
·
IF 3.8
Zhou Ning,Chen Linlin,Zhang Xing,He Kunmiao,Xu Tiantian,Wang Hanshuo,Sun Jie,Yu Miao,Ren Hongtao,Wang Na
Proanthocyanidins play a crucial role in celiac disease (CD) through their antioxidant and anti-inflammatory properties, effectively regulating oxidative stress and inflammatory responses. However, the poor stability and low bioavailability of proanthocyanidins significantly limit their therapeutic applications. The aim of this study was to investigate the effects of lyoprotectants on proanthocyanidin nanoliposome freeze-dried powder (PCNL-FD), characterize its structural properties, evaluate its in vitro antioxidant capacity and explore its protective effects in a celiac disease cell model. The addition of 4% trehalose as a lyoprotectant effectively maintained the encapsulation efficiency and redispersion stability of PCNL-FD. FTIR and TEM analyses confirmed the successful incorporation of proanthocyanidins into the phospholipid bilayer. In vitro antioxidant assays showed that PCNL-FD exhibited significantly higher DPPH, ABTS, and hydroxyl radical scavenging activities compared to free proanthocyanidins. In the p31-43 peptide-induced celiac disease Caco-2 cell model, PCNL-FD significantly reduced oxidative stress by decreasing ROS and MDA levels while increasing GSH levels and antioxidant enzyme activities (SOD and CAT). The formulation showed anti-inflammatory effects by inhibiting pro-inflammatory cytokines (TNF-α, IL-6) and promoting anti-inflammatory cytokines (IL-4, IL-10). In addition, PCNL-FD activated the Keap1/Nrf2 signaling pathway and upregulated the expression of NQO-1 and HO-1. This nanoliposomal delivery system effectively overcame the bioavailability and stability limitations of free proanthocyanidins, providing new insights into the management of oxidative stress in inflammatory diseases such as celiac disease.
研究领域:
分子生物学
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药剂学
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免疫学
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消化病学
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营养学
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纳米医学
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氧化应激
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 24
·14438-14447
·
IF 3.108
Lin Zhou,Yujie Wu,Youfei Cheng,Jie Wang,Jun Lu,Jinyan Gao,Juanli Yuan,Hongbing Chen
Celiac disease (CD) is a T cell-mediated enteropathy of the small intestine and caused by the ingestion of wheat gluten and related prolamins in barley and rye. However, there is no effective therapy to alleviate symptoms of celiac disease except for a life-long gluten-free diet. Recent studies showed that modification by microbial transglutaminase (mTG) could reduce the gliadin-specific immune response. In the present study, different acyl-acceptor substrates in combination with mTG were used to modify the model 33-mer peptide (LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF), which is a particular celiac toxic α-gliadin peptide. RP-HPLC and LC-ESI-MS were performed to test the extent of the modifications. R5 ELISA and G12 ELISA were used to analyze the antigenicity of the modified peptide. The shifts of retention time and molecular weight showed great modification of 33-mer peptide after 2 h of incubation with mTG. When acyl-acceptor substrates were crosslinked with 33-mer peptide, the antigenicity of modified peptide forms was decreased compared to its initial level. In summary, it is demonstrated that mTG is active on a variety of chemically acyl-acceptor substrates. Transamidation by mTG with an appropriate amine donor can be used to block the antigenicity of gliadin peptide related to celiac disease. These findings highlight a potential strategy to prevent cereal toxicity in celiac disease.
研究领域:
免疫学
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食品科学
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胃肠病学
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分子生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF APPLIED ELECTROCHEMISTRY
·
2023
·
IF 2.9
Zhan Ke,Du Yongkun,Liu Qingqing,Cui Chenxu,Xing Guangxu,Li Shanshan,Ren Hongtao,Wang Fan,Xu Chao,Wang Na
Wheat gliadin is thought to be the main cause of wheat allergy leading to celiac disease. In this study, we reported an electrochemical immunosensor based on AuNPs/Zn/Ni-ZIF-8-800@graphene for simple, efficient, and sensitive detection of gliadin. A monoclonal antibody against gliadin were prepared by hybridoma technique. Zn/Ni-ZIF-8-800 with high stability was prepared by using zinc–nickel bimetallic organic framework MOFs, and graphene and gold nanoparticles with large surface area and excellent conductivity were introduced. Chitosan was used as a binder to fixed the composite on the surface of glassy carbon electrode. The fabricated materials and the corresponding sensors were comprehensively characterized by X-ray diffractometer, scanning electron microscope, transmission electron microscopy, Fourier transform infrared spectroscopy, Brunauer–Emmett–Teller, and cyclic voltammetry analyses. Under the optimal conditions, the linear range of the electrochemical immunosensor was 0.1–100 μg mL −1 , and the detection limit was 0.950 μg mL −1 . In addition, the fabricated sensor exhibited high selectivity and long-term stability. The recovery test showed that the prepared sensor was suitable for the detection of gliadin in real food and feed, exhibiting great promising of the developed sensor in practical application. Graphical abstract
研究领域:
分析化学
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生物化学
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材料科学
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食品科学
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免疫学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）