Frontiers in Oncology
·
2021
·
IF 6.244
Li Xiaoyu,Xia Shujun,Ji Ri,Zhan Weiwei,Zhou Wei
Objectives: A novel ultrasound contrast agent (UCA) VEGFR2-targeting iron-doped silica (SiO2) hollow nanoparticles (VEGFR2-PEG-HSNs-Fe NPs) was prepared and applied in microwave ablation for breast cancer to investigate its value in the evaluation of effectiveness after tumor ablation.Methods: VEGFR2-PEG-HSNs-Fe NPs were prepared by using nano-SiO2, which was regarded as a substrate and etched by ferrous acetate, and then modified with anti-VEGFR2 antibody. Laser confocal microscope and flow cytometry were used to observe its main physicochemical properties, and biological safety was also investigated. After the xenograft tumor was treated with microwave ablation, the extent of perfusion defect was evaluated by ultrasound by injecting VEGFR2-PEG-HSNs-Fe NPs.Results: The average particle size of VEGFR2-PEG-HSNs-Fe was 276.64 ± 30.31 nm, and the surface potential was −13.46 ± 2.83 mV. In vitro, the intensity of ultrasound signal increased with UCA concentration. Good biosafety was performed in in vivo and in vitro experiments. The enhanced ultrasound signal was detected in tumors after injection of VEGFR2-PEG-HSNs-Fe NPs, covering the whole tumor. The lesions, which were incompletely ablated, presented as contrast agent perfusion at the periphery of the tumor, and contrast enhanced ultrasound (CEUS) was performed again after complementary ablation. It was confirmed that all the lesions were completely ablated.Conclusion: Nano-targeted UCAs VEGFR2-PEG-HSNs-Fe NPs had good biosafety and ability of specific imaging, which might be used as a contrast agent in CEUS to evaluate the efficacy of tumor ablation.
研究领域:
医学影像学
·
肿瘤学
·
生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2025
·
IF 6.5
Jing Zhang,Min Zha,Shanghua Xiao,Nina Filipczak,Satya Siva Kishan Yalamarty,Xiangping Wu,Chenkai Gong,Xiang Li
Objective Nanoparticles constructed with silver/manganese dioxide (Ag@MnO2) as the core, in conjunction with survivin siRNA (sis) and cyclo(RGD-DPhe-K) (Ag@MnO2-sis-c-L), were prepared for integrated tumor diagnosis and therapy.Methods Ag@MnO2-sis-c-L particles were prepared and characterized. The silver and manganese content were determined by inductively coupled plasma optical emission spectroscopy (ICP-OES). The stability of sis in the system was evaluated by incubation with 50% FBS before the agarose gel electrophoresis experiment. The in vitro photothermal conversion ability, cytotoxicity to 4T1 cells, and cellular uptake of preparations were evaluated. The dialysis technique was employed to determine the in vitro release profile of Ag and Mn from Ag@MnO2-sis-c-L under various pH conditions. The pharmacokinetic behavior and tissue distribution of silver in vivo were detected by ICP-OES. Animal model experiments were conducted to further evaluate the anti-tumor efficacy of Ag@MnO2-sis-c-L against breast cancer in combination with infrared irradiation.Results Our newly synthesized Ag@MnO2-sis-c-L nanoparticles displayed superior physicochemical properties. The combined application of these nanoparticles with photothermal therapy (PTT) exerted the strongest synergistic inhibitory effects on tumor growth. Survivin protein expression in tumor tissues were markedly suppressed following delivery of nanoparticles loaded with sis. Additionally, magnetic resonance imaging revealed the high imaging capability of hybrid nanoparticles.Conclusion This study supports the potential utility of Ag@MnO2-sis-c-L coupled with PTT in therapeutic and diagnostic imaging applications.
研究领域:
纳米医学
·
肿瘤学
·
生物材料
·
分子影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2018
·
IF 4.556
Chu Tang,Yang Du,Qian Liang,Zhen Cheng,Jie Tian
The ability to detect breast cancer early in its progression is essential to improve patient survival and quality of life. The noninvasive and dynamic imaging and functional assessments of estrogen receptor-alpha (ERα), which is commonly expressed at high levels in breast cancer, are important for effective diagnosis and treatment. Hence, the development of a specific ERα-targeted probe is a major research goal. To that end, in the present study, we created a novel near-infrared (NIR) fluorescent probe, IRDye800CW–E2, for targeted ERα imaging in breast-tumor-bearing mice. IRDye800CW–E2 consisted of a cyanine dye IRDye800CW as the NIR fluorophore and the E2 analogue ethinyl estradiol amine as an ERα targeting ligand. The ethinyl estradiol amine was initially labeled with fluorescein isothiocyanate (FITC) to evaluate the binding specificity to human breast-tumor cells in vitro. Flow chamber and in vitro confocal laser endomicroscopy imaging experiments demonstrated that FITC–E2 was specifically taken up by MCF-7 cells. Furthermore, NIR fluorescence imaging revealed the ability of IRDye800CW–E2 to rapidly target tumors and to achieve good contrast between tumors and background signal 4–48 h postinjection. The fluorescent signal of IRDye800CW–E2 in tumors was successfully blocked by the coinjection of the endogenous ERα-ligand 17β-estradiol (E2) and the probe. Ex vivo fluorescent imaging further confirmed high uptake of the probe by tumors. These results indicated that IRDye800CW–E2 has great potential as an ERα-targeted imaging probe for early breast-tumor detection and has potential for clinical translation.
研究领域:
医学影像学
·
肿瘤学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Science of Advanced Materials
·
2021
·Vol. 13
·期 9
·1611-1621
·
IF 1.474
Guo Rong,Ma Binbin,Nie Jiaqiu
This study aimed to explore the value of preoperative mammography in the differential diagnosis of benign and malignant tumors of nipple discharge. A biocompatible T1 contrast agent KMnF3 nanoparticle was first developed in the research, and then RGD-coupled KMnF3
nanoparticles were further synthesized as a highly sensitive tumor-targeted magnetic resonance imaging (MRI) contrast agent. While the nanoparticle was characterized physically, cytotoxicity test and MRI test in breast cancer mice were performed, and the excised tumors were subjected to immunostaining
and tumor electron microscope section processing. At the same time, 60 patients with nipple discharge were screened to participate in the research, and the prepared MRI nano contrast agent was used for the differential diagnosis of breast benign/malignant tumors of nipple discharge. In the
experiment, the synthetic nanoparticles were tested by Fourier transformed infrared (FTIR), which proved that the designed RGDtu/KMnF3 nanoparticles were successfully synthesized. The quantitative analysis of the synthesized nanoparticles showed that the relaxation efficiency reached
23.12 mM−1s−1, and there was no obvious toxicity. After staining, the microscope showed that the tumor was proliferating. After intravenous injection of low-dose RGDtu/KMnF3 contrast agent, nanoparticles were found in the tumor tissue. It was found
that the synthesized nanoparticles enhanced the contrast of tumors with a volume of less than 50 mm3 by observing tumor slices. The imaging of the patient’s breast showed that the X-ray classification of galactography based on this contrast agent was statistically significant
in distinguishing benign/malignant lesions of nipple discharge (X
2 = 58.700, P < 0.01).
研究领域:
医学影像学
·
生物材料学
·
肿瘤学
✓ 已验证
DOI
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Tingting Hu,Lu Jia,Heng Li,Chunyu Yang,Yuening Yan,Huiming Lin,Feng Zhang,Fengyu Qu,Wei Guo
Phototherapy and sonodynamic therapy (SDT) are widely used for the synergistic treatment of tumors and have received considerable attention. However, an inappropriate tumor microenvironment, including pH, H 2 O 2 , oxygen, and glutathione levels, can reduce the therapeutic effects of synergistic phototherapy and SDT. Here, a novel Bi-based soluble microneedle (MN) is designed for the CT imaging of breast tumors and starvation therapy/gas therapy-enhanced phototherapy/SDT. The optimized Bi/BiVO 4 Schottky heterojunction serves as the tip of the MN, which not only has excellent photothermal conversion ability and CT contrast properties, but its heterojunction can also avoid the rapid combination of electrons and hole pairs, thereby enhancing the photodynamic/sonodynamic effects. A degradable MN with excellent mechanical properties is fabricated by optimizing the ratios of poly(vinyl alcohol), poly(vinyl pyrrolidone), and sodium hyaluronate. Glucose oxidase (GO x ) and diallyl trisulfide are loaded into the MN to achieve tumor starvation and gas therapy, respectively; And the controlled release of GO x and H 2 S can be achieved under ultrasound or near-infrared laser irradiation. The in vitro and in vivo results demonstrate that this multifunctional MN can achieve high therapeutic efficacy through starvation therapy/gas therapy-enhanced phototherapy/SDT. The designed multifunctional MN provides a prospective approach for synergistic phototherapy and SDT.
研究领域:
生物医学工程
·
肿瘤学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 31
·6877-6885
·
IF 5.344
Qingbing Wang,Xiaoxia Guo,Yi Chen,Zhiyuan Wu,Yu Zhou,Saima Sadaf,Liang Han,Xiaoyi Ding,Tao Sun
Biomimetic materials are attracting increasing attention in the field of drug delivery due to their low immunogenicity, good biocompatibility and degradability. Furthermore, the modification of endogenous species on the surfaces of biomimetic materials can generate new biomedical functions. A real-time understanding of the drug-release behavior, especially in vivo, can provide reliable information for drug activation and distribution. Herein, we describe a theranostics system caged in human serum albumin (HSA) as a therapy against breast tumors. The prodrug of a drug–dye conjugate (DDC) was successfully covalently linked into HSA via a disulfide-exchange reaction to give a high drug-loading rate. The drug-release and fluorescence recovery curves are in good agreement, allowing the drug-release kinetics to be monitored by a non-invasive fluorescence signal in real time. A disintegration mechanism triggered by GSH was suggested. The DDC@HSA particles show tumor-targeting capability, and good antitumor efficacy in vitro and in vivo. Hopefully, this strategy can provide a generalizable novel approach for the facile encapsulation of drugs for engaging HSA, paving the way for precision diagnosis and therapy in preclinical trials.
研究领域:
药物递送
·
生物材料
·
肿瘤学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2024
·
IF 4.4
Xiaoqing Lin,Asim Mushtaq,Muhammad Suhail,Yike Hou,Guohui Jing,Muhammad Arshad,M.Zubair Iqbal,Xiangdong Kong
Breast cancer is one of the most challenging malignancies in the present era, which continues to attract researchers to discover new therapeutic routes to combat tumors. Photodynamic therapy (PDT) has been promised to deal with malignancies as well as bacterial infections during surgery or afterward. In this study, copper doped titanium dioxide nanocomposites (Cu-TiO 2 NCs) are synthesized and modified by an FDA-approved triblock polymer (Pluronic® F-127) to enhance the biocompatibility and stability of NCs. PF-127 coating increased the hydrodynamic particle sizes to 190 nm for 1Cu-TiO 2 @PF-127 and 220 nm for 10Cu-TiO 2 @PF-127, while Cu doping raised the zeta potential to 6.64 mV, which shifted to −6.64 mV and −0.08 mV after PF-127 functionalization, indicating effective surface modification. This nano-assembly generated significant amount of reactive oxygen species and demonstrated 90 % cancer cell death after low-intensity UV irradiation (5.6 mW·cm −2 ), while maintaining more than 70 % survival of normal L929 cells, indicating low toxicity to healthy cells. Moreover, antibacterial analysis against Escherichia coli and Staphylococcus aureus bacterial strains proved excellent antibacterial features of Cu-TiO 2 NCs. Based on such outcomes, it can be expected that Cu-TiO 2 NCs have potential for future nanomedicines.
研究领域:
肿瘤学
·
纳米医学
·
生物材料科学
✓ 已验证
DOI
高影响因子
International Journal of Nanomedicine
·
2016
·
IF 4.32
Ping Sun,Wei Huang,Mingji Jin,Qiming Wang,Bo Fan,Lin Kang,Zhonggao Gao
Nanoparticle-mediated small interfering RNA (siRNA) delivery is a promising therapeutic strategy in various cancers. However, it is difficult to deliver degradative siRNA to tumor tissue, and thus a safe and efficient vector for siRNA delivery is essential for cancer therapy. In this study, poly(ethylene glycol)-modified chitosan (PEG-CS) was synthesized successfully for delivering nucleic acid drug. We deemed that PEGylated CS could improve its solubility by forming a stable siRNA loaded in nanoparticles, and enhancing transfection efficiency of siRNA-loaded CS nanoparticles in cancer cell line. The research results showed that siRNA loaded in PEGylated CS (PEG-CS/siRNA) nanoparticles with smaller particle size had superior structural stability in the physical environment compared to CS nanoparticles. The data of in vitro antitumor activity revealed that 4T1 tumor cell growth was significantly inhibited and cellular uptake of PEG-CS/siRNA nanoparticles in 4T1 cells was dramatically enhanced compared to naked siRNA groups. The results from flow cytometry and confocal laser scanning microscopy showed that PEG-CS/siRNA nanoparticles were more easily taken up than naked siRNA. Importantly, PEG-CS/siRNA nanoparticles significantly reduced the growth of xenograft tumors of 4T1 cells in vivo. It has been demonstrated that the PEG-CS is a safe and efficient vector for siRNA delivery, and it can effectively reduce tumor growth and prevent metastasis.
研究领域:
癌症治疗
·
药学
·
纳米技术
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER SCIENCE
·
2023
·Vol. 61
·期 12
·1111-1119
·
IF 3.4
Yajun Xu,Jianlin Lv,Chaoying Kong,Yanran Li,Kun Wang,Na Shen,Zhaohui Tang
Tirapazamine (TPZ, 3-amino-1,2,4-benzotriazine 1,4-dioxide), a representative hypoxia-activated prodrugs (HAPs), has entered Phase II/III clinical trials. Despite a promising phase II clinical trial results, TPZ does not bring benefits to cancer therapy in most phase III clinical trials, which making the clinical trials failed. To improve its therapeutic effects, in this study, we synthesize a novel TPZ derivative, 3-(3-(5-hydroxypentyl)ureido)benzo[e][1,2,4]triazine 1,4-dioxide (TPZH), and covalently conjugated it to poly (L-glutamic acid) (PLG) to obtain TPZH nanoparticles (TPZH-NPs). The TPZH-NPs shows prolonged blood circulation, enhanced tumor accumulation, and increased maximum tolerated dose (MTD) than TPZH. In addition, TPZH-NPs exhibits significantly enhanced antitumor therapeutic efficiency than free TPZH against 4T1 breast tumors. Graphical
研究领域:
药学
·
肿瘤学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Yongdie Deng,Fenghua Zhang,Menglu Jiang,Yanju Liu,Huiping Yuan,Jinsong Leng
4D printing is an advanced manufacturing technology combining additive manufacturing with smart materials. Based on light-active shape memory composites, smart medical structures with remote control capability, therapeutic function, and biocompatibility are hopefully fabricated by 4D printing. Here, a multifunctional composite with good mechanical properties, biocompatibility, and light-active shape memory performance is prepared by incorporating gold nanoparticles into a shape memory polyurethane matrix. The composites demonstrate a rapid and stable light-thermal effect, which can achieve localized and controlled breast tumor ablation, providing an approach to hyperthermia treatment for cancer cells. By directly bioprinting the composite melt, a series of 4D-printed structures are manufactured accurately in a convenient, clean, and safe way, which show a fast autonomous light-driven shape recovery process. The examples of a 4D-printed soft tissue scaffold and intraluminal scaffold can expand from a conveniently insertional shape to an expanded shape under light exposure. The proposed strategies provide great inspiration for customized multifunctional light-thermal therapeutic structures for minimally invasive treatment.
研究领域:
生物医学工程
·
材料科学
·
癌症治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Wenjing Yang,Yibing Ji,Chenhang Ding,Xujie Zhang,Wurikaixi Aiyiti,Chongxian He,Cijun Shuai
Reactive oxygen species (ROS) mediated tumor therapeutic strategies have been extensively studied. However, its effectiveness in eliminating tumor cells is inevitably restricted by the hypoxia or the insufficient hydrogen peroxide (H 2 O 2 ) content. To fundamentally address these challenges, an O 2 -independent and stimuli-responsive nanosystem (named CuS@PMS) is ingeniously constructed by loading peroxymonosulfate (PMS: HSO 5 − ) into hollow mesoporous copper sulfide (CuS) to mediate the generation of sulfate radicals (SO 4 − ). The CuS@PMS was then introduced into polycaprolactone (PCL) to prepare into breast meshes by selective laser sintering. Specifically, the released Cu 2+ ions combining with the photothermal property of CuS synergistically activate PMS, ensuring the cascade amplification of SO 4 − . Unlike conventional ROS, the production of SO 4 − independent on the content of O 2 and H 2 O 2 in the tumor microenvironment, characterizing a longer half-life and faster reaction rate, thereby possessing more advantages in antitumor. The results showed that the SO 4 − yield of PCL/CuS@PMS group significantly higher than that of PCL/PMS group, especially under near-infrared (NIR) light. Moreover, the antitumor efficiency of PCL/CuS@PMS + NIR group was 43 % higher than that of PCL/PMS group in an O 2 -independent manner. The study provided a more efficient, highly adaptable and versatile approach for tumor treatment.
研究领域:
生物医学工程
·
纳米技术
·
肿瘤治疗
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
·
2010
·Vol. 15
·期 4
·591-599
·
IF 3.415
Zhu Hua,Huang Liliang,Zhang Yuanqing,Xu Xiaoping,Sun Yanhong,Shen Yu-Mei
To develop technetium- and rhenium-labeled nonsteroidal estrogen imaging agents for estrogen receptor (ER) positive breast tumors, two groups of rhenium and technetium cyclofenil derivatives were synthesized and characterized. The binding affinities of the rhenium complexes for ERs were determined. The tricarbonyl rhenium complex showed the highest binding affinity for ERs (81.2 for ERβ, 16.5 for ERα). Tricarbonyl technetium cyclofenil complexes were obtained in high radiochemical purity and radiochemical yields. The results of studies of their octanol/water partition and in vitro stability are presented. These results demonstrate that these radiolabeled cyclofenil derivatives may be considered as potential breast cancer imaging agents.
研究领域:
医学影像学
·
药物化学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）