INTERNATIONAL DAIRY JOURNAL
·
2023
·
IF 3.1
Xi Cao,Miao Qiao,Yayu Pan,Qingbin Bao,Guiquan Zhang,Yikun Xian
The fatty acid (FA) composition of giant panda colostrum and the variation during lactation were determined. Of the 35 FAs detected in giant panda milk fat , the most abundant were oleic acid , palmitic acid and linoleic acid . There were 14 very long-chain and 5 short- and medium-chain FAs detected. The ratio of n-6 to n-3 FAs was 3.60–7.21. Hierarchical clustering analysis demonstrated there was an unusually slow transition from colostrum to mature milk, probably an adaptive strategy. The oleic acid/linoleic acid ratio was higher in colostrum than mature milk, which may benefit digestion. Most saturated FAs were more abundant in mature milk than colostrum. Arachidonic acid accounted for 48.49% of very long-chain polyunsaturated FAs in colostrum, which was 3.34 fold that in mature milk, and for docosahexaenoic acid , 2.75 fold. During the lactation, Spearman correlations within unsaturated and saturated FAs were generally positive; correlations between them were generally negative.
研究领域:
动物学
·
营养学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Yue Jia,Yu Lu,Xue Wang,Yuerong Yang,Meiyi Zou,Jie Liu,Wanjun Jin,Xiaoqin Wang,Guoliang Pang,Linjuan Huang,Zhongfu Wang
Bovine lactoferrin is a functional N-glycoprotein whose glycan moieties play an important role in its biological activities. Although bovine lactoferrin is rich in sialylated N -glycans, there are no reports on the differentiation between α2,3-linked and α2,6-linked sialylated N- glycan isomers. In this study, bovine lactoferrin was separated and purified by cation exchange chromatography, and >95% pure protein was obtained. Following this, N- glycan derivatives of lactoferrin at different stages of lactation were qualitatively and quantitatively analyzed by MALDI-TOF-MS and HILIC-MS/MS. With prolonged lactation, the relative content of the sialylated and fucosylated bi-antenna complex N -glycan (Hex1Man3GlcNAc4GalNAc1Fuc1Neu5Ac1) changed the maximum, with 14.5%, 1.3%, and 0.9% contents in transitional milk, colostrum, and mature milk, respectively. Meanwhile, the contents of sialylated and fucosylated N -glycans in transitional milk were the hi ghest . More importantly, the sialylated N- glycans-linked isomers could be distinguished by linkage-specific derivatization of sialic acid . There were 11 α2,3- and 13 α2,6-linked sialyation N- glycans isomers in the colostrum, which transformed to 4 and 3 N- glycan isomers, respectively, in the mature milk. These findings provide a foundation for an in-depth research on the structure–function relationship of bovine lactoferrin.
研究领域:
生物化学
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营养学
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分子生物学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Yuyang Zhang,Tianjiao Han,Lan Zhang,Shuai Yun,Yue Yuan,Xiaoqin Wang,Linjuan Huang,Zhongfu Wang,Yu Lu
Goat lactoferrin is an N-glycoprotein, and its glycan moieties are essential for its biological activity. However, the structures of these glycans, particularly the sialylated isomers with α-2,3- or α-2,6-linkages, remain poorly characterized. Utilizing online hydrophilic liquid chromatography-tandem mass spectrometry, our study characterized N-glycans in goat lactoferrin across different lactation stages, and the putative structures of the 86 N-glycans of goat lactoferrin were presented, including 53 previously undetected ones. The content and variety of N-glycans decreased from colostrum to mature milk, with transitional milk exhibiting the highest levels of neutral and high-mannose N-glycans. Colostrum was particularly rich in fucose- and sialylated N-glycans, especially those with α-2,6-linkages. Notably, the α-2,6-linked sialylated N-glycan H5N4F1A1-1 was 7.09-fold and 12.85-fold more abundant in colostrum compared to transitional and mature milk, respectively. These findings provide valuable insights into the structure of lactoferrin and could facilitate the development of functional goat milk products.
研究领域:
生物化学
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营养学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Animals
·
2019
·Vol. 9
·期 12
·1019
·
IF 1.832
Keyuan Liu,Yang Li,Guobin Luo,Hangshu Xin,Yonggen Zhang,Guangyu Li
Simple SummaryThe objective of this study was to determine the relationships between milk odd- and branched-chain fatty acids (OBCFAs) and ruminal fermentation parameters, microbial populations, and base contents. Significant relationships existed between the concentrations of C11:0,iso-C15:0,anteiso-C15:0, C15:0, andanteiso-C17:0 in rumen and milk. The total OBCFA content in milk was positively related to the acetate molar proportion but negatively correlated with isoacid levels. The adenine/N ratio was negatively related to milk OBCFA content but positively associated with theiso-C15:0/iso-C17:0 ratio.AbstractThe purpose of this research was to evaluate whether relationships exist between odd- and branched-chain fatty acids (OBCFAs) originating from milk fat and the corresponding data of ruminal fermentation parameters, microbial populations, and base contents that were used to mark microbial protein in rumen. Nine lactating Holstein dairy cows with similar body weights and parity were selected in this study, and the samples of rumen and milk were collected at the early, middle, and late stages, respectively. The rumen and milk samples were collected over three consecutive days from each cow, and the ruminal and milk OBCFA profiles, ruminal fermentation parameters, bacterial populations, and base contents were measured. The results showed that the concentrations of OBCFAs, with the exception of C11:0 and C15:0, were significantly different between milk and rumen (p< 0.05). The concentrations ofanteiso-fatty acids in milk were higher than those in rumen, and the contents of linear odd-chain fatty acids were higher than those of branched-chain fatty acids in both milk and rumen. Significant relationships that existed between the concentrations of C11:0,iso-C15:0,anteiso-C15:0, C15:0, andanteiso-C17:0 in rumen and milk (p< 0.05). The total OBCFA content in milk was positively related to the acetate molar proportion but negatively correlated with isoacid contents (p< 0.05). The populations ofRuminococcus albus,R. flavefacients, andEubacterium ruminantiumwere significantly related to milk C13:0 contents (p< 0.05). The adenine/N ratio was negatively related to milk OBCFA content (p< 0.05) but positively associated with theiso-C15:0/iso-C17:0 ratio (p< 0.05). Milk OBCFAs were significantly correlated with ruminal fermentation parameters, ruminal bacterial populations, and base contents. Milk OBCFAs had the potential to predict microbial nitrogen flow, and the prediction equations for ruminal microbial nitrogen flow were established for OBCFAs in dairy milk.Keywords:fermentation parameters;microbial populations;microbial bases;odd- and branched-chain fatty acids;lactation stages
研究领域:
动物营养学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2022
·Vol. 13
·期 18
·9674-9686
·
IF 6.317
Qiu-Ye Lan,Si-Yu Huang,Chen-Yu Jiang,Meng-Tao Yang,Tong Wu,Xiao-Yan Chen,Zhao-Yan Liu,Wei Wei,Xing-Guo Wang,Hui-Lian Zhu
Triacylglycerol (TAG) is the primary constituent of human milk fat and plays a vital role in the healthy development of infants. But few studies reported the sophisticated profile of TAG molecular species in human breast milk and its temporal changes during a prolonged lactation period. An efficient ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) method was adopted to examine TAGs. A total of 128 TAGs in 296 human breast milk samples collected during postnatal 0 to 400 days were identified. The changes in the human milk TAG profile mainly took place in the early stages of lactation (postnatal 0–45 days), and the TAG profile became stable in mature milk after 200 days of lactation. Odd chain fatty acids (OC-FAs) may be important markers for identifying human breast milk of different lactation stages. This study could provide evidence for developing safe and efficacious human-milk substitutes for children without access to human breast milk.
研究领域:
营养学
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生物化学
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分析化学
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DOI
PubMed
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2023
·Vol. 32
·期 7
·903-909
·
IF 2.9
He Yangzheng,Chen Li,Liu Wenqun,Deng Ze-yuan,Li Jing
In this study, gas chromatography–mass spectrometry (GC–MS) coupled with Partial least squares Discriminant Analysis (PLS-DA) was used to analyze the volatiles in Chinese breast milk from different cities (Wuhan, Qingdao and Hohhot) and different lactation stages (colostrum and mature milk). The results showed that breast milk contained 122 volatile substances in 9 major groups, with the largest number of olefins (36) and the highest content of acids. The different volatile compounds of breast milk in three cities were heptanal, 2-pentylfuran, (E)-2,4-decadienal, (E)-2-heptenal, (E)-2-nonenal and 1-octen-3-one, colostrum and mature milk were (E)-2-heptenal, (E)-2-decenal, lauric acid, n -decanoic acid, (E)-2-nonenal and octanoic acid. This study might provide scientific data for the development and optimization of formulas that were more suitable for the health of Chinese infants.
研究领域:
营养学
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分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2020
·Vol. 11
·期 2
·1869-1880
·
IF 4.171
Xinyue Dai,Tinglan Yuan,Xinghe Zhang,Qin Zhou,Huiya Bi,Renqiang Yu,Wei Wei,Xingguo Wang
This study aimed to quantify the short-chain fatty acids (SCFAs) and medium-chain fatty acids (MCFAs) in human milk triacylglycerols (TAGs) and investigate their concentrations in human milk consumed during lactation by infants born at different gestational ages. One hundred and eighty milk samples from the mothers of 30 full-term, 10 early-preterm (≤32 weeks), 10 mild-preterm (32–34 weeks), and 10 near-term (34–37 weeks) infants were collected from the colostrum, transitional, and mature milk. The human milk TAGs were transferred into fatty-acid methyl esters via potassium methoxide in methanol and determined using gas chromatography (GC). The total SCFA (4:0) and MCFA concentrations (6:0 and 8:0) were highest in the mature milk (1.47 ± 0.66 mg g−1 fat from full-term infant milk), approximately 42.18% higher than those in transitional milk. Significantly higher SCFA and MCFA concentrations were found in full-term milk than in preterm milk (p = 0.001). The milk TAGs were analyzed using ultra-high-performance supercritical fluid chromatography with quadrupole-time-of-flight mass spectrometry (UHPSFC-Q-TOF-MS), which showed that the SCFAs and MCFAs were mainly esterified with long-chain fatty-acid groups (16:0, 18:1 n-9, and 18:2 n-6) at the glycerol backbone. The infants’ daily SCFA intake from human milk was estimated; this was highest from mature milk for full-term infants (∼14 mg d−1) which was significantly different from that of preterm infants from colostrum and transitional milk (p < 0.001). The correlation between dietary SCFAs and MCFAs in human milk and nutrition in newborns, especially in the gut microbiotas of preterm infants, requires further study.
研究领域:
营养学
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儿科
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分析化学
·
婴儿健康
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DOI
PubMed
高影响因子
Food Bioscience
·
2022
·
IF 5.318
Cuirong Ren,Jun Jin,Shiwei Zhao,Yanbing Zhang,Qingzhe Jin,Xingguo Wang
Effects of five different breeds (Landrace × Large White, Landrace , Large White, Duroc and Pietrain) and two lactation stages (colostrum and milk) on phospholipids and their fatty acids compositions , cholesterol, and tocopherols in sow milks (n = 100) were investigated in the present study. Six classes of phospholipids were identified using 31 P NMR spectroscopy, with the total phospholipid levels ranging from 56.84 to 151.02 μmol/100 mL. Sphingomyelin (SM) was the most abundant phospholipids (33.57%–48.00%) in both colostrum and milk. Colostrum contained higher percentages of SM, phosphatidylserine (PS), phosphatidylinositol (PI), and ethanolamine plasmalogen (EPLAS) than those in milk, while milk contained higher percentages of phosphatidylcholine (PC), and phosphatidylethanolamine (PE). C16:0 and C18:0, accounting for more than 80% of the saturated fatty acids of phospholipids in both colostrum and milk, decreased throughout the lactation. The average cholesterol concentration in colostrum was 430.60 mg/100 g fat, which was significantly lower than that in milk (646.44 mg/100 g fat). α-Tocopherol levels in Landrace × Large White breed were 38.35 and 15.91 mg/100 g fat in colostrum and milk, respectively, which were significantly higher than other breeds; while no significant variations in β-, γ-, and δ-tocopherols were noted among different breeds. The results demonstrated that lactation stages and breeds were mainly responsible for the significant differences of phospholipids, cholesterol, and tocopherols, providing a theoretical guidance for the manufacture of commercial formula of piglets.
研究领域:
动物科学
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营养学
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生物化学
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产品关联
DOI
高影响因子
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