PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
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2018
·Vol. 285
·期 1883
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IF 4.847
Yaoyu Jiao,Xiaoyun Hu,Yufa Peng,Kongming Wu,Jörg Romeis,Yunhe Li
The area planted with insect-resistant genetically engineered crops expressing Bacillus thuringiensis (Bt) genes has greatly increased in many areas of the world. Given the nearby presence of non-Bt crops (including those planted as refuges) and non-crop habitats, pests targeted by the Bt trait have a choice between Bt and non-Bt crops or weeds, and their host preference may greatly affect insect management and management of pest resistance to Bt proteins. In this study, we examined the oviposition preference of the target pest of Bt rice, Chilo suppressalis, for Bt versus non-Bt rice plants as influenced by previous damage caused by C. suppressalis larvae. The results showed that C. suppressalis females had no oviposition preference for undamaged Bt or non-Bt plants but were repelled by conspecific-damaged plants whether Bt or non-Bt. Consequently, C. suppressalis egg masses were more numerous on Bt plants than on neighbouring non-Bt plants both in greenhouse and in field experiments due to the significantly greater caterpillar damage on non-Bt plants. We also found evidence of poorer performance of C. suppressalis larvae on conspecific-damaged rice plants when compared with undamaged plants. GC-MS analyses showed that larval damage induced the release of volatiles that repelled mated C. suppressalis females in wind tunnel experiments. These findings suggest that Bt rice could act as a dead-end trap crop for C. suppressalis and thereby protect adjacent non-Bt rice plants. The results also indicate that the oviposition behaviour of target pest females should be considered in the development of Bt resistance management strategies.
研究领域:
农业昆虫学
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植物保护
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2025
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IF 4
Fang-Rui Guo,Shu-Chao Wang,Yan Liu,Shuai Wang,Jing-Mei Huang,Hao Sun,Lin-Feng He,Yuan Xie,Song-Tao Qiao,Feng-Xia Yang,Chris Bass,Cong-Fen Gao,Shun-Fan Wu
The development of insecticide resistance in insect populations is a major challenge to sustainable agriculture and food security worldwide. Methoxyfenozide, an insect growth regulator that acts as an agonist of 20-hydroxyecdysone (20E), has severely declined in its efficacy against the rice stem borer ( Chilo suppressalis ), a notorious pest of rice crops in East and Southeast Asia. To date, however, the genes involved in methoxyfenozide resistance in target pests remain unclear. We conducted a long-term (seven years from 2017 to 2023) and large geographical scale (8 provinces and 45 cities in China) resistance monitoring program for methoxyfenozide in C. suppressalis . Resistance was seen to arise rapidly in this species, with >100-fold resistance being detected in nearly all the field populations after 2018 . Piperonyl butoxide (PBO), an inhibitor of cytochrome P450 enzymes (P450s), significantly increased the sensitivity of resistant strains of C. suppressalis to methoxyfenozide, implicating P450s in resistance. Six P450 genes: CYP321F3, CYP6CV5, CYP9A68 , CYP6AB45, CYP324A12 and CYP6SN2 were identified as highly expressed in resistant C. suppressalis by transcriptome profiling. Of these, ectopic expression of CYP321F3 in Drosophila melanogaster resulted in a 7.0-fold increase in resistance to methoxyfenozide demonstrating its causal role in resistance. Collectively, these findings provide insight into the mechanisms mediating resistance to insect growth regulators and will inform the development of future pest and resistance management strategies.
研究领域:
昆虫学
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分子生物学
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农业科学
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DOI
PubMed
高影响因子
Environmental Science-Nano
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2024
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IF 5.8
Ao Liang,Yunzheng Zhang,Xiang Xu,Hao Wang,Changwei Gong,Jie Hu,Xiangsong Li,Jizhi Yang,Anchun Peng,Xuegui Wang
Chilo suppressalis is a major pest that severely impacts rice production in China. However, the widespread use of insecticides has resulted in the development of resistance in C. suppressalis. The advancement of nanotechnology offers promising prospects for enhancing insecticide formulations and improving their efficacy. This study designed a pH-responsive release system composed of γ-PGA and chitosan (CS) loaded with chlorantraniliprole (CLAP). The synthesized CLAP-loaded nanoparticles had an average particle size of approximately 39.67 nm and a loading efficiency of 38.87%. Under a pH of 8.5, 64.4% of the pesticide was released within 120 hours. The CLAP@CS/γ-PGA formulation, after loading, exhibited a significant synergistic insecticidal effect, with bioassay results showing an 82.2% mortality rate of C. suppressalis six days post-treatment. Tests of metabolic genes and enzyme activities showed that CLAP@CS/γ-PGA rendered C. suppressalis more sensitive to insecticides by inhibiting the activities of P450 and by decreasing the expression of CYP9A68. CLAP@CS/γ-PGA also demonstrated favorable transport properties within C. suppressalis and rice plants, and due to the encapsulation by the nanoparticle carrier, it reduced toxicity to zebrafish. In summary, the system we investigated not only meets the needs of pest management but also enhances the utilization of pesticides.
研究领域:
农业科学
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环境科学
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纳米技术
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DOI
高影响因子
PEST MANAGEMENT SCIENCE
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2018
·Vol. 75
·期 3
·718-725
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IF 3.249
Lu Xu,Jun Zhao,Yang Sun,Dejin Xu,Guangchun Xu,Xiaolong Xu,Yueliang Zhang,Shuijin Huang,Zhaojun Han,Zhongyan Gu
BACKGROUND The rice striped stem borer (SSB), Chilo suppressalis (Walker), which is one of the most economically important phytophagous pests, has developed resistance to multiple insecticides. The resistance of SSB against chlorantraniliprole has been investigated in detail. However, the mechanism of its metabolic resistance has rarely been studied. RESULTS A field population from Wuhu City, China was used to establish chlorantraniliprole resistant and susceptible strains (WHR and WHS) by laboratory continuous selection. Enzyme activities data suggested the potential involvement of cytochrome P450 monooxygenase in WHR. CYP6CV5 , CYP9A68 , CYP321F3 , and CYP324A12 were significantly overexpressed in WHR (from 4.48 to 44.88-fold). These four P450 genes were expressed in the late developmental stages of WHR; however, they were almost absent during the egg stage. In addition, their expressions were much more sensitive to chlorantraniliprole induction in WHR than in WHS. Injection of individual and mixture dsRNAs reduced the expression of the four target genes (55.2–73.2% and 43.2–50.2%, respectively) and caused significant larvae mortality (55.1–65.1% and 88.2%, respectively). CONCLUSION Multiple overexpressed P450 genes were potentially associated with chlorantraniliprole resistance, as confirmed by the RNA interference (RNAi) assay. Our findings suggested that metabolic resistance to chlorantraniliprole might be mediated by P450s. © 2018 Society of Chemical Industry
研究领域:
昆虫学
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分子生物学
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害虫管理
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DOI
PubMed
高影响因子
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
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2025
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IF 3.7
Shuai Wang,Yuan Xie,Chong Liu,Jin-Wei Liu,Fang-Rui Guo,Song-Tao Qiao,Yu-Ji Xing,Shun-Fan Wu,Chris Bass,Cong-Fen Gao
Abamectin serves as a key alternative for managing diamide-resistant Chilo suppressalis . However, resistance to abamectin itself presents a significant challenge to this pest control, and its genetic basis remains poorly understood. In this study, we report an increase in field resistance to abamectin in populations of C. suppressalis and provide evidence for a cytochrome P450-mediated metabolic resistance mechanism, supported by enzyme activity assays and synergist bioassays. Transcriptomic profiling analysis revealed six candidate P450 genes, among which the duplicated gene CYP6SN2 showed a strong correlation with resistance. Variant CYP6SN2vB was highly expressed in detoxification tissues and mainly produced isoforms with a short 3′-UTR. Functional validation in transgenic Drosophila melanogaster confirmed that overexpression of CYP6SN2vB conferred 2.4- and 1.9-fold resistance to abamectin and emamectin benzoate, whereas CYP6SN2vA had no detectable effect. Molecular docking further revealed that CYP6SN2vB could potentially hydroxylate abamectin via interactions near oxygen-binding motifs. In contrast, the inward-folded substrate recognition site 1 region of CYP6SN2vA compresses the ligand-binding cavity, precluding abamectin interaction. These findings suggest neofunctionalization of CYP6SN2vB following duplication and provide valuable insights into the molecular basis of abamectin resistance in C. suppressalis , with implications for resistance management.
研究领域:
昆虫学
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杀虫剂抗性
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分子生物学
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遗传学
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生物信息学
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毒理学
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2022
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IF 4.966
Ming-yang Li,Chang-wei Gong,Yun-zheng Zhang,Xia Zhao,Yan Jia,Jian Pu,Xue-mei Liu,Xiang Xu,Xue-gui Wang
Chilo suppressalis Walker (Lepidoptera: Crambidae) is one of the most destructive pests occurring in the rice-growing regions of Asia. Parasitoids , mainly egg parasitoids , have been of interest for several years even with practical used cases. Therefore, the potential impact of insecticides on natural enemies needs great attention. In this study, chlorantraniliprole was evaluated for its impact on C. suppressalis and two dominant parasitic wasps . Bioassays showed that chlorantraniliprole had negligible toxicity to Eriborus terebrans but was significantly toxic to Chelonus munakatae ; the mortality exceeded 50% when the concentration reached 46.83 ng/cm 2 . Enzyme assays suggested that the significantly different carboxylesterase activity may be involved in the high-level detoxification metabolism of E. terebrans . According to the results of enzyme gene correlation analysis, P450s may be the dominant factor in the detoxification metabolism of C. munakatae . In addition, the ryanodine receptor C-terminus of C. suppressalis (CsRyR), C. munakatae (CmRyR) and E. terebrans (EtRyR) were successfully cloned. Different amino acids at resistance mutation I4758 M between susceptible C. suppressalis (I) and parasitic wasps (M) may be related to susceptibility differences. Simulated docking showed that CsRyR and CmRyR can interact with chlorantraniliprole but not EtRyR. More interaction forces were formed between CsRyR and chlorantraniliprole than CmRyR. Furthermore, a Pi-Pi T-shape formed between 73PHE in CsRyR and the benzene ring in chlorantraniliprole. These results indicated that both detoxification metabolism and the target site could mediate the susceptibility difference between C. suppressalis and its parasitic wasps.
研究领域:
昆虫学
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毒理学
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生物化学
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分子生物学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2023
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IF 9.5
Kefei Zhao,Guangchun Xu,Leng Wang,Tianyue Wu,Xingyu Zhang,Chenhui Zhang,Yuhang Zhao,Zilu Li,Yuxia Gao,Fengpei Du
Various strategies have been developed to improve the applicability of hydrophobic pesticides for better effectiveness in agriculture. However, existing formulations of hydrophobic pesticides still suffer from complicated processing, abused organic solvents, indispensable surfactants, or inescapable ecotoxicity, which strictly limit their applications. Herein, a dynamic covalent bond tailored pesticide (fipronil) amphiphile is constructed to address the above issues, which accomplishes the nanodispersion, full wetting, and precise delivery without organic solvents, surfactants, and materials simultaneously. By introducing a hydrophilic ligand on the hydrophobic fipronil through an imine bond, the cleavable fipronil amphiphile (FPP) exhibits superior water solubility and can even self-assemble into micelles at higher concentrations, which can be directly applied in powder form without organic solvents. Attributed to the suitable hydrophilic/hydrophobic ratio, FPP achieves full wetting and effective deposition on superhydrophobic rice leaves without surfactants. Moreover, benefiting from the unique dynamic nature of the imine bond, FPP maintains good storage stability while sensitively releasing back to fipronil under the humidity and pH trigger, consequently implementing the precise delivery for nontarget Apis cerana and target Chilo suppressalis without materials. To our knowledge, this dynamic covalent bond tailored amphiphile strategy is the first idea that simultaneously takes the dispersibility, wettability, and responsiveness of hydrophobic pesticides into account, providing a possibility to control the entire journey of field application and even promising to be incorporated into the synthesis process, thus paving the way for modern sustainable agriculture.
研究领域:
农业科学
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材料科学
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化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）