JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Yihan Wang,Jie Zheng,Yubin Xue,Bo Yu
Dipicolinic acid (DPA), a cyclic diacid, has garnered significant interest due to its potential applications in antimicrobial agents, antioxidants, chelating reagents, and polymer precursors. However, its natural bioproduction is limited since DPA is only accumulated in Bacillus and Clostridium species during sporulation. Thus, heterologous production by engineered strains is of paramount importance for developing a sustainable biological route for DPA production. Pseudomonas putida KT2440 has emerged as a promising host for the production of various chemicals thanks to its robustness, metabolic versatility, and genetic tractability. The dominant Entner–Doudoroff (ED) pathway for glucose metabolism in this strain offers an ideal route for DPA production due to the advantage of NADPH generation and the naturally balanced flux between glyceraldehyde-3-phosphate and pyruvate, which are both precursors for DPA synthesis. In this study, DPA production via the ED pathway was in silico designed in P. putida KT2440. The systematically engineered strain produced dipicolinate with a titer of 11.72 g/L from glucose in a 5 L fermentor. This approach not only provides a sustainable green route for DPA production but also expands our understanding of the metabolic potential of the ED pathway in P. putida KT2440.
研究领域:
微生物学
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生物化学
·
代谢工程
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Yue-Yang Ma,Zi-Jian Wang,Dong-Jin Qian
Detection of anthrax biomarker dipicolinic acid (DPA) is of great importance upon the crisis of bioterrorism. Development of fluorescent materials for DPA detection, particularly one that fully depends on single luminescent response, faces the challenge of being susceptible to interferences. The accompanying accuracy problems offer great opportunities for the establishment of more reliable ratiometric analysis method. Herein, a ratiometric fluorescent probe based on terbium functionalized graphitic carbon nitride nanosheets (Tb-g-C 3 N 4 NS) is attempted for quantitative detection of DPA to address the distinct function of g-C 3 N 4 NS as both carrier and reference fluorophore, which is a so-far unexplored option in fluorescent detection approaches. We achieve the incorporation of Tb 3+ into framework of g-C 3 N 4 NS by using a simple synthetic strategy comprised of thermal pyrolysis and ultrasonic exfoliation. Combining the reference signal over g-C 3 N 4 NS at 440 nm (I 440 ) with the response signal of Tb 3+ at 546 nm (I 546 ), concentration of DPA can be easily calculated via its linear correlation with the intensity ratio (I 546 /I 440 ), giving a precise measurement towards DPA with a detection limit as low as 9.9 nM. Besides enabling an excellent self-calibrating detection of DPA, this work also inspires broader use of g-C 3 N 4 NS for relevant process.
研究领域:
生物化学
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分析化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Composites and Hybrid Materials
·
2025
·Vol. 8
·期 3
·1-11
·
IF 21.8
Lin Xiaodong,Cao Zhengyang,Zeng Shiyu,Zhu Haoyu,Zhai Kairui,Yin Binfeng,Zhang Cheng,Peng Tao,Cheng Teng,Zhang Biao
In this work, we developed a dual-mode portable device that integrated a 3D-printed microfluidic chip for detection of dipicolinic acid (DPA) on chip. The system uses a ratiometric fluorescence nanoprobe formed by embedding carbon dots (CDs) into an Eu 3 ⁺ metal–organic framework (Eu-MOF). Upon reaction with DPA in the microchannel, red fluorescence was enhanced and blue fluorescence suppressed, enabling sensitive ratiometric detection of DPA on chip with a detection limit (LOD) of 0.04 µM. Interestingly, the composite Eu MOF /CDs/DPA also exhibits peroxidase-like activity, catalyzing the oxidation of TMB into a blue-colored product (oxTMB), which allows for colorimetric detection with an LOD of 10.14 µM. To improve usability and reduce environmental or instrumental variability, incorporating a microfluidic chip into a semi-portable device and utilizing a smartphone, making the system portable and miniaturized for easy operation. In the smartphone-assisted mode, the LODs were 0.33 µM (ratiometric fluorescence) and 12.27 µM (colorimetry), determined by RGB signal analysis, respectively. Moreover, satisfactory recoveries (85–104.6%) were achieved in the spiked real samples. Overall, this platform offers a straightforward, cost-effective, and versatile approach for DPA detection, with promising applications in food safety, environmental monitoring, and clinical diagnostics.
研究领域:
分析化学
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生物医学工程
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2020
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IF 5.339
Qiuju Zhou,Yajun Fang,Jiayu Li,Dan Hong,Panpan Zhu,Shihong Chen,Kejun Tan
In this work, a novel dual-ratiometric optical probe based on europium-doped carbon dots (Eu-CDs) was developed for colorimetric and fluorescent visual sensing dipicolinic acid (DPA), a anthrax biomarker. Eu-CDs with recessive signals were prepared via thermal pyrolysis method. And the magenta appears when Eu-CDs coordinates with Eriochrome Black T (EBT). Based on the ligand displacement strategy, DPA reduces magenta and makes blue appear, which develop ratiometric colorimetric visual detection methods of DPA. Under 270 nm excitation, DPA make the red fluorescence signal of Eu in EBT-CDs@Eu appear, based on the absorbance-energy transfer-emission effect, the ratiometric fluorescent visual detection assay of DPA has been developed. Based above, dual-ratiometric optical probe was designed successfully. The limit of detection (LOD) is 10.6 nM for ratio colorimetric assay . More prominently, naked-eye determination of DPA without instrument is as low as 1.0 μM. Furthermore, its practicability was verified by quantifying the DPA in Bacillus subtilis spores and human urine (with RSD≤2.35%). The sensor shows great superiority in on-site analysis of DPA, especially in limited instruments conditions.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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