CHEMICAL ENGINEERING SCIENCE
·
2025
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IF 4.3
Usman Farooq,Xudong Hu,Jingyi Shang,Shuang Li,Jun Long,Lin Wang,Xinhai Wang
The increasing presence of pharmaceutical contaminants, such as dipyrone (DIP), in water systems highlights the urgent need for innovative and sustainable remediation technologies. This study examined the degradation of DIP using a chalcopyrite (CP)-activated peracetic acid (PAA) system, highlighting its potential for the removal of pharmaceutical pollutants. Experimental and statistical parameter optimization suggested that approximately 85.5 % of DIP degradation and 60 % of mineralization were achieved with CP (4 g/L) and PAA (10 mM) at neutral pH. Scavenger and probe experiments confirmed the presence of hydroxyl radicals ( • OH) and alkyl radical species in the CP/PAA system, but •OH was the primary contributor to DIP degradation. The surface-active sites confirmed that heterogeneous Cu(I) exerted more significant influence than Fe (II) of CP, producing 4.21 and 2.35 mg/L, respectively. The presence of reductive sulfur species in the CP allowed the capture of additional protons, creating sulfur vacancies and further strengthening the cycling of Cu and Fe species. DFT analysis confirmed that the FeCuS 2 -Fe adsorption energy (−3.71 eV) and the HOMO-LUMO gap of FeCuS 2 -Fe (0.03 eV) were critical to produce radicals. The CP/PAA system is suitable for DIP degradation and contributes significantly to the degradation of other environmental pollutants. It retained its capacity to degrade above 65 % for up to five consecutive cycles, offering a new and innovative method for water treatment.
研究领域:
环境科学
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化学工程
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材料科学
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产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2021
·
IF 5.097
Shengnan Yin,Changlun Tong
Detection of Bacillus anthracis spores is of great importance for biosecurity and prevention of disease outbreaks. In this paper, a ratiometric colorimetric and fluorescent dual-mode platform for rapid, sensitive, and visual detection of dipicolinic acid (DPA), a biomarker of anthrax spores, was developed based on Eu3+-modified silver nanoparticles (AgNPs). Eu3+-modified AgNPs can induce the aggregation of AgNPs, its localized surface plasmon resonance (LSPR) absorption at 392 nm is decreased, and a new absorption peak at 524 nm emerged. Interestingly, upon the addition of DPA, it can effectively inhibit the aggregation of AgNPs–Eu3+, and the solution color changes from pink to yellow, while the red characteristic fluorescence of Eu3+ is turned on owing to the energy transfer from DPA to Eu3+ by the antenna effect. A new insight that AgNPs can also enhance the fluorescence of Eu3+ was found. Eu3+-modified AgNPs as a dual-mode platform for ratiometric colorimetric and fluorescence detection of DPA were thus fabricated. The good linearity is in the range of 1.0–25 μM with a LOD of 0.31 μM for the ratiometric colorimetric assay and 0.25–45 μM with a LOD of 17 nM for the fluorescent assay, which is greatly lower than the dose of DPA (60 μM) released by the contagious number of spores to cause anthrax. The ratiometric colorimetric and fluorescent probes were successfully applied for the detection of DPA in lake waters and Bacillus subtilis, and as low as 1.8 × 104 spores were detected.
研究领域:
生物安全
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分析化学
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环境科学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2014
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IF 3.84
Huihui Chen,Zhiqiang Liu,Duxia Cao,Shanshan Lu,Jingzhong Pang,Yunhui Sun
Two new fluorescence turn-on chemosensors for cyanide based on dipyridylamine substituted aurone moiety have been synthesized. The strong yellow-green fluorescence of the compounds were completely quenched after the addition of Cu(II) ions because of the complexation between the compounds and Cu(II) ions and were almost recovered after the addition of cyanide owing to the strong bonding ability between cyanide and Cu(II) ions, which lead to the removal of copper ions from the complexes. The chemosensors exhibit good repeatability.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
Biosensors-Basel
·
2022
·Vol. 12
·期 8
·668
·
IF 5.743
Xiang Li,Junsong Wu,Huaguang Hu,Fangfang Liu,Jialian Wang
A desirable lanthanide-based ratiometric fluorescence probe was designed as a multifunctional nanoplatform for the determination of dipicolinic acid (DPA), a unique bacterial endospore biomarker, with high selectivity and sensitivity. The carbon dots (CDs) with blue emission wavelengths at 470 nm are developed with europium ion (Eu3+) to form Eu3+/CDs fluorescent probes. DPA can specifically combine with Eu3+and then transfer energy from DPA to Eu3+sequentially through the antenna effect, resulting in a distinct increase in the red fluorescence emission peak at 615 nm. The fluorescence intensity ratio of Eu3+/CDs (fluorescence intensity at 615 nm/fluorescence intensity at 470 nm) showed good linearity and low detection limit. The developed ratiometric nanoplatform possesses great potential for application in complex matrices owing to its specificity for DPA. In addition, the integration of a smartphone with the Color Picker APP installed enabled point-of-care testing (POCT) with quantitative measurement capabilities, confirming the great potential of the as-prepared measurement platform for on-site testing.Keywords:ratiometric;fluorescence;determination of dipicolinic acid;smartphone-based detection
研究领域:
生物化学
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分析化学
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微生物学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
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2024
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IF 4
Dongxu Yi,Yuhang Dong,Minghui Qi,Minghung Hong,Bin Zhu,Guobin Ren
Drug development necessitates substantial investments in both human and material resources. In the advanced stages of development, significant solubility challenges frequently emerge, critically influencing a drug's applicability and efficacy. Crystal engineering, particularly through the application of cocrystal technology, presents a novel strategy to address these solubility issues without compromising the drug's effectiveness. However, the success rate of cocrystals formation remains relatively low. This article evaluates the impact of widely used virtual cocrystal screening methods on prediction outcomes, especially in the context of conformational diversity. The study describes the effects of eight dipyridamole (DIP) conformations and 130 cocrystal formers (CCFs) using molecular complementarity and molecular electrostatic potential methods, while the hydrogen bond propensity method did not account for conformations variations. Findings indicate that different DIP conformations lead to variable prediction outcomes in virtual cocrystal screening. Thus, it is crucial consider the conformational states of APIs and CCFs in virtual screenings to enhance prediction accuracy. By comparing virtual screening results with experimental results, this study demonstrated that incorporating conformational considerations can improve cocrystal success rates, minimize experimental workload, and contribute to energy-conservation and emission-reduction. Additionally, this research provides the true positives and true negatives data of DIP's cocrystal, laying the groundwork for further studies.
研究领域:
药学
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化学信息学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
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IF 5.7
Yihan Wang,Jie Zheng,Yubin Xue,Bo Yu
Dipicolinic acid (DPA), a cyclic diacid, has garnered significant interest due to its potential applications in antimicrobial agents, antioxidants, chelating reagents, and polymer precursors. However, its natural bioproduction is limited since DPA is only accumulated in Bacillus and Clostridium species during sporulation. Thus, heterologous production by engineered strains is of paramount importance for developing a sustainable biological route for DPA production. Pseudomonas putida KT2440 has emerged as a promising host for the production of various chemicals thanks to its robustness, metabolic versatility, and genetic tractability. The dominant Entner–Doudoroff (ED) pathway for glucose metabolism in this strain offers an ideal route for DPA production due to the advantage of NADPH generation and the naturally balanced flux between glyceraldehyde-3-phosphate and pyruvate, which are both precursors for DPA synthesis. In this study, DPA production via the ED pathway was in silico designed in P. putida KT2440. The systematically engineered strain produced dipicolinate with a titer of 11.72 g/L from glucose in a 5 L fermentor. This approach not only provides a sustainable green route for DPA production but also expands our understanding of the metabolic potential of the ED pathway in P. putida KT2440.
研究领域:
微生物学
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生物化学
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代谢工程
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DOI
PubMed
高影响因子
CHEMICAL PAPERS
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2019
·Vol. 73
·期 7
·1753-1759
·
IF 1.246
Xu Li,Zhang Ya-Nan,Ji Xing-Hu,He Zhi-Ke
Silicon nanodots (SiNDs) as an excellent fluorescent nanomaterial have got more and more applications. In this work, the terbium-modified silicon nanodots (SiNDs-Tb) were synthesized and successfully applied to the detection of dipicolinic acid (DPA), a biomarker of anthrax spores. Here, SiNDs serve as scaffolds and a fluorescence reference ( F 445 ), coordinating with Tb 3+ ions, while terbium ions as specific recognition elements and response signals ( F 490 and F 547 ) with zero background fluorescence. The sensing process was easily operated in aqueous media and can be finished in a short time. With a concentration range of 0.2–10 μM of DPA, the fluorescence intensity ratio ( F 547 / F 445 ) correspondingly generated quantitative changes and the limit of detection was 24 nM. It is a rapid and sensitive sensing system and provides a new approach for full exploitation of SiNDs in fluorescence assay.
研究领域:
生物化学
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纳米生物学
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分析化学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
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IF 8.5
Sheng Wang,Jun Xu,Fengzhi Yue,Lina Zhang,Ning Bi,Jian Gou,Yongxin Li,Yuanyuan Huang,Tongqian Zhao,Lei Jia
Dipicolinic acid (DPA), as a biomarker for Bacillus anthracis , is highly toxic at trace levels. Rapid and on-site quantitative detection of DPA is essential for maintaining food safety and public health. This work develops a dual-channel self-calibrated fluorescence sensor constructed by the YVO 4 :Eu and Tb- β -diketone complex for rapid visual detection of DPA. This sensor exhibits high selectivity , fast response time, excellent detection sensitivity, and the detection limit is as low as 4.5 nM in the linear range of 0–16 μM. A smartphone APP and portable ultraviolet lamp can assemble a mobile fluorescence sensor for on-site analysis. Interestingly, adding Cu 2+ ions can quench the fluorescence intensity of Tb 3+ . In contrast, the addition of cysteine can restore the fluorescence, allowing the accurate detection of Cu 2+ ions and cysteine in environmental water and food samples. This work provides a portable sensor that facilitates real-time analysis of multiple targets in food and the environment.
研究领域:
生物化学
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食品安全
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环境科学
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DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Jing Wang,Daquan Li,Yu Qiu,Xinyue Liu,Liang Huang,Huimin Wen,Jun Hu
We reported Eu(III) functionalized carbon dots (CDs-Eu) as a novel ratiometric fluorescent probe for point-of-care testing (POCT) of dipicolinic acid (DPA), a biomarker of anthrax. The probe was synthesized simply by coordination interaction between Eu(III) ions and the CDs with carboxyl and amino groups. Upon addition of DPA, the intrinsic red luminescence of Eu(III) ions can be sensitized due to the presence of energy transfer when DPA chromophore coordinated with Eu(III) ions. The fluorescence peaking at 530 nm as a reference remained essentially constant, leading to ratiometric fluorescence response toward DPA. This approach exhibited a linearity range from 0.5 nM to 5 μM and a detection limit of 0.8 nM. More Importantly, the combination of probe-based test paper and smartphone provided a convenient approach for visual POCT of DPA with a detection limit of 67 nM, which was lower than other reported test paper for DPA detection. The color of fluorescence gradually changed from green to red as the DPA increases, which can be captured by a smartphone and further quantitatively analyzed with a built-in Color Analyzer App. The satisfactory result of detecting DPA accurately and reliably in real samples by the test paper certified its practicality in POCT of DPA.
研究领域:
分析化学
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医学诊断
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生物传感
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Meng Li Liu,Bin Bin Chen,Jia Hui He,Chun Mei Li,Yuan Fang Li,Cheng Zhi Huang
The highly sensitive detection of dipicolinic acid (DPA), a biomarker of the biological threat-agent anthrax, is strongly associated with the sensing of Bacillus anthracis ( B. anthracis ) in environmental and food samples. In this study, we developed a novel, ultrasensitive method for the detection of DPA by using a ratiometric fluorescent terbium ions modified carbon dots (CDs-Tb). The CDs-Tb showed two fluorescent emission bands at 459 nm and 495 nm when excited at the single wavelength of 260 nm. DPA could specifically bind with terbium ions on the surface of CDs through strong chelate-conjugation to produce antenna effect, resulting in significantly enhancement of the 495 nm emission peak without affecting the 459 nm emission peak. The fluorescence intensity ratio ( I 495 / I 459 ) of CDs-Tb was proportional to the concentration of DPA in the range of 0.5 nM to 2.5 μM with the limit of detection as low as 100 pM. This selective and ultrasensitive assay had a great application prospect in the complex matrixes owing to its simplicity and specificity for DPA. Meanwhile, the CDs-Tb-based paper sensor was successfully developed for sensitive and visual detection of DPA.
研究领域:
生物化学
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环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Lan-Fang Pang,Hao Wu,Meng-Xia Wei,Xiao-Feng Guo,Hong Wang
The spores of Bacillus anthracis are highly deadly to human beings and animals, and are concurrently potential biological warfare agents. Hence, the rapid and sensitive monitoring Bacillus anthracis biomarker, dipicolinic acid (DPA), is very desirable. Herein, orange/green dual-emissive carbon dots (OG-CDs) were synthesized via the hydrothermal approach. The OG-CDs not only emitted dual fluorescence at 527 and 590 nm under the single 503 nm excitation, but also exhibited excellent water solubility, good photostability and great salt tolerance. The fluorescence of the OG-CDs at 527 nm can be completely quenched when chelated with Cu(II). However, because of the stronger chelation between DPA and Cu(II), the fluorescence restored rapidly on subsequent addition of DPA. As such, the CD-Cu(II) system can be used for determination of DPA based on the fluorescence “off-on” response. Under optimum conditions, the detection limit for DPA was 56 nM, with a linear range of 0.5–12.5 μM. The established CD-Cu(II) based spectrofluorometric method has been applied to the analysis of DPA in real water samples with recoveries of 93.6%–104.3%. More remarkably, the CD-Cu(II) probe also has been successfully applied for the imaging of DPA in Escherichia coli with excellent bio-compatibility.
研究领域:
生物化学
·
分析化学
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Yue-Yang Ma,Zi-Jian Wang,Dong-Jin Qian
Detection of anthrax biomarker dipicolinic acid (DPA) is of great importance upon the crisis of bioterrorism. Development of fluorescent materials for DPA detection, particularly one that fully depends on single luminescent response, faces the challenge of being susceptible to interferences. The accompanying accuracy problems offer great opportunities for the establishment of more reliable ratiometric analysis method. Herein, a ratiometric fluorescent probe based on terbium functionalized graphitic carbon nitride nanosheets (Tb-g-C 3 N 4 NS) is attempted for quantitative detection of DPA to address the distinct function of g-C 3 N 4 NS as both carrier and reference fluorophore, which is a so-far unexplored option in fluorescent detection approaches. We achieve the incorporation of Tb 3+ into framework of g-C 3 N 4 NS by using a simple synthetic strategy comprised of thermal pyrolysis and ultrasonic exfoliation. Combining the reference signal over g-C 3 N 4 NS at 440 nm (I 440 ) with the response signal of Tb 3+ at 546 nm (I 546 ), concentration of DPA can be easily calculated via its linear correlation with the intensity ratio (I 546 /I 440 ), giving a precise measurement towards DPA with a detection limit as low as 9.9 nM. Besides enabling an excellent self-calibrating detection of DPA, this work also inspires broader use of g-C 3 N 4 NS for relevant process.
研究领域:
生物化学
·
分析化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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