LUMINESCENCE
·
2023
·Vol. 38
·期 8
·1458-1464
·
IF 2.9
Hui-Ting Cao,Tiansheng Zhao,Meng-Qian Liu,Li-Zhen Guo,Yong-Qin He,Kun Zhang,Xi Mai,Na Li
Sensing of pyrophosphate ion (PPi) has received much attention due to the strong demand for clinical diagnostics. Here, based on gold nanoclusters (Au NCs), a ratiometric optical detection method for PPi is developed by simultaneously detecting the dual signals of fluorescence (FL) and second-order scattering (SOS). The PPi is detected by inhibiting the formation of aggregates of Fe 3+ with Au NCs. Binding of Fe 3+ to Au NCs causes aggregation of Au NCs, which leads to fluorescence quenching and scattering increasing. The presence of PPi can competitively bind Fe 3+ to re-disperse the Au NCs and finally recover the fluorescence and reduce the scattering signal. The designed PPi sensor shows a high sensitivity with a linear range 5–50 μM and a detection limit of 1.2 μM. In addition, the assay has excellent selectivity for PPi, which makes its application in real biological samples extremely valuable.
研究领域:
分析化学
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生物医学工程
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纳米技术
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 18
·4375-4382
·
IF 3.637
Zhang Wen Jie,Liu Shi Gang,Zhang Xing Yue,Luo Hong Qun,Li Nian Bing
Ratiometric assays, which can effectively surmount external interference, have attracted extensive research interests. Herein, a novel ratiometric sensing platform for Hg 2+ is designed based on nitrogen-doped carbon dots (N-CDs) with two different optical signals. Under a single excitation, N-CDs have two emission peaks around 668 nm and 412 nm, which are second-order scattering and fluorescence, respectively. Upon the addition of Hg 2+ , the weak scattering emission at 668 nm can be increased apparently, while the strong fluorescence intensity at 412 nm is weakened. Moreover, the ratio of scattering intensity to fluorescence intensity is linearly dependent on Hg 2+ concentration (0.1–10 μM and 10–30 μM, respectively), and the detection limit is 66 nM. In addition, the ratiometric sensing mechanism is investigated in detail, which is due to the combined effect of aggregation-induced fluorescence quenching and scattering enhancement. Furthermore, the developed sensing approach holds a promising application for Hg 2+ detection in actual samples.
Graphical abstract
研究领域:
分析化学
·
环境科学
·
材料科学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Zhihao Wu,Danyang Nan,Huan Yang,Shuang Pan,Hui Liu,Xiaoli Hu
A facile ratiometric fluorescence method for ascorbic acid (AA) detection was established by combining the fluorescence of MoS 2 quantum dots (QDs) with second-order scattering (SOS) of CoOOH. In MoS 2 QDs/CoOOH nanohybrid system, the fluorescence of MoS 2 QDs was quenched by CoOOH whereas the intensity of SOS was relatively close to the fluorescence due to the intrinsic high scattering ability of large size CoOOH nanoflakes . With the addition of AA, CoOOH nanoflakes were reduced to Co 2+ accompanied the size decline and the disappearance of characteristic absorption in UV–vis spectrum, resulting in the fluorescence recovery and SOS fading. Thus, the ratiometric detection of AA was obtained through the opposite changes of fluorescence and SOS. Under optimized conditions, the detection limit of AA was 0.21 μmol L −1 with a linear range from 0.80 to 32.0 μmol L −1 . Furthermore, the MoS 2 QDs/CoOOH nanohybrid system was applied to the detection of AA in human serum samples and vitamin C tablets with satisfactory results.
研究领域:
分析化学
·
生物医学检测
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DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 42
·15353-15359
·
IF 4
Peng Zhang,Liming Chen,Xiaoyan Cai,Binbin Luo,Tianju Chen,Haini Chen,Guoliang Chen,Feiming Li
A traditional fluorescence–scattering intensity based ratiometric sensing system utilizes both inherent scattering and fluorescence intensity and has drawn extensive attention owing to its simplicity and self-calibration properties. In this work, we propose a novel ratiometric fluorescence sensing system that combines a fluorescence wavelength shift and scattering in a single window, using second-order scattering (SOS) as the representative scattering signal based on the halide exchange of CsPbBr3@SiO2 perovskite nanocrystal composites. We observe a fast halide exchange within 10 seconds, resulting in an identifiable fluorescence wavelength blue shift, while the scattering wavelength remains relatively constant for self-correction. This system could be applied for ratiometric sensing of Cl− in the serum without any sample treatment. The established wavelength-based ratiometric system demonstrates high reliability and reproducibility, paving a new way for fluorescence sensing.
研究领域:
分析化学
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纳米技术
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生物医学工程
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Xinyue Chen,Dan Liu,Huifang Wu,Jiahui Ji,Zhiyuan Xue,Shilan Feng
A novel photoluminescent Hcy-AuNCs has been developed through one-pot reduction method, to establish a tobramycin sensing by second-order scattering (SOS). Hcy-AuNCs could spontaneously assemble to small-scaled aggregation, resulting in remarkable intensity enhancement of scattered luminescence signals. The luminescence of Hcy-AuNCs could be clearly observed under ultraviolet lamp, when excited at 365 nm, a significant luminescent intensity at 741 nm was monitored in SOS spectra. The introduction of AuNPs would cause large-scaled aggregation of Hcy-AuNCs that was rapidly settled in the solution, resulting in the decrease of SOS intensity. Besides, the non-radiative energy transfer between AuNPs and Hcy-AuNCs would also reduce the luminescent intensity. However, the addition of tobramycin would cause the aggregation of AuNPs due to the electrostatic and covalent bonding between AuNPs and tobramycin, thus eliminating the interference of AuNPs. The luminescence of Hcy-AuNCs reappeared, exhibiting an optical response toward tobramycin. The good linearity was obtained in a wide range from 4 nM to 300 nM with a low detection limit of 0.27 nM. The selectivity was acceptable toward different types of antibiotics. Finally, the proposed method was successfully applied to the widely used tobramycin eye drops.
研究领域:
分析化学
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纳米技术
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药物科学
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DOI
PubMed
高影响因子
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