Food Chemistry-X
·
2026
·
IF 9.2
Hui Teng,Minyi Yan,Chong Chen,Chao Ai,Huangjuan Huo,Lei Chen
In this study, chitosan-modified dihydromyricetin liposomes (CHI-DMY-Lips) were developed via electrostatic adsorption to overcome the low bioavailability of dihydromyricetin (DMY). Employing conventional DMY liposomes (DMY-Lips) as controls, a comprehensive assessment was conducted on the physicochemical properties, activity preservation, in vitro release kinetics, as well as the structural stability and bioaccessibility during simulated gastrointestinal (GI) digestion of the delivery system. CHI-DMY-Lips exhibited a positive surface charge with larger size, more uniform distribution, and higher encapsulation efficiency (54.00% compared to 41.01% in DMY-Lips). The encapsulation by liposomes preserved the antioxidant activity of DMY, whereas CHI further augmented its activity through synergistic effects. CHI-DMY-Lips presented enhanced structural and electrostatic stability during storage and superior in vitro sustained-release performance (especially at pH 7.4). Besides, CHI-DMY-Lips retained structural integrity throughout GI digestion and preserved its effective concentration and activity, resulting in a bioaccessibility approximately threefold that of free DMY (25.97% vs. 9.73%).
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Foods
·
2026
·Vol. 15
·期 14
·2441
·
IF 6
Hui Xiang,Qin Li,Longchen Shang,Xiujuan Chen,Lingli Deng,Yexing Tao
In this study, composite nanofibrous membranes composed of poly(vinyl alcohol) (PVA), gelatin, and zein loaded with different contents of dihydromyricetin (DMY) were fabricated via electroblowing spinning (EBS). The effects of DMY content on the microstructure, physicochemical properties, mechanical strength, and functional performance of the membranes were evaluated. Scanning electron microscopy (SEM) analysis showed that the average fiber diameter increased from 174 ± 29 nm to 221 ± 35 nm with increasing DMY content, followed by a slight decrease at higher loading levels, indicating that DMY incorporation influences fiber morphology. Fourier transform infrared spectroscopy (FTIR) results suggested the presence of hydrogen bonding interactions between DMY and the polymer matrix. X-ray diffraction (XRD) and differential scanning calorimetry (DSC) results indicated changes in the physical state of DMY within the nanofibrous system as the loading content increased. All samples exhibited a typical two-stage release behavior, and the highest cumulative release (nearly 55%) was observed at a DMY loading of 22.5%, while further increasing the loading reduced the release efficiency to approximately 45%. The release profiles were well described by a first-order kinetic model. The composite membranes exhibited improved surface hydrophilicity, appropriate water vapor permeability, antioxidant activity, and antibacterial activity againstEscherichia coli(E. coli) andStaphylococcus aureus(S. aureus). This study demonstrates the successful fabrication of DMY-loaded PVA/gelatin/zein nanofibrous membranes and provides preliminary insights into their structure–property–function relationships, release behavior, antioxidant activity, and antibacterial activity against representative bacteria, although further application-oriented validation is still required.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Jingjing Zhang,Hui Cao,Bing Li,Fuying Wang,Qin Duan,Yaru Li,Jinjin Pei,Hui Wang,Juan Carlos Mejuto,Jesús Simal-Gándara,Haolin Zhang
Heterocyclic amines (HCAs) are carcinogenic contaminants formed during meat thermal processing, and the traditionally recognized inhibitory effects of flavonoids on HCA formation remain controversial. Dihydromyricetin (DMY) and baicalein (BAI) both exhibited dose-dependent bidirectional effects on PhIP formation in a PhIP chemical model. At low concentrations (DMY 125–250 μM, BAI 125–1000 μM), PhIP levels gradually increased to nearly 210% and 300% of control, respectively, mainly driven by amination-related reactions of flavonoid thermal degradation products that promoted phenylacetaldehyde accumulation. At high concentrations (≥2000 μM), PhIP was suppressed to <50% (DMY) and <20% (BAI) of control via potent radical scavenging and phenylacetaldehyde trapping. The bidirectional regulation may be governed by a dynamic balance between amination-driven promotion and the dual inhibitory pathways for PhIP formation. This study explains how flavonoids lead to contradictory effects on HCA formation in cooked meat and provides a mechanistic foundation for their application.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Gerui Ren,Ruiqi Hu,Jiajun Mao,Xinpei Cai,Tianrong Wang,Xin Song,Wentao Wu,Caixin Zhuge,Min Huang,Hujun Xie,Kejun Cheng
Dihydromyricetin (DMY) possesses potent antioxidant, anti-inflammatory and neuroprotective activities, yet its potential applications are curtailed by extremely low aqueous solubility and limited bioaccessibility. To overcome these constraints, the Zein-DMY/κ-carrageenan (κ-CGN) nanoparticles were engineered via anti-solvent precipitation at pH 5 (zein:κ-CGN mass ratio of 6:4) that reached 83.1% encapsulation efficiency. Multispectral analysis (FTIR, fluorescence, XRD) combined with molecular docking showed that hydrogen bonding, electrostatic and hydrophobic interactions were the main driving forces for the formation of nanoparticles, and DMY was anchored in the hydrophobic region of Zein. The dense sulfate groups of κ-CGN electrostatically stabilized the surface of nanoparticles, preventing their aggregation. Compared with free DMY, the nanocomposite retained strong antioxidant activity (95.25% for DPPH and 97.20% for ABTS), increased water solubility 4.88-fold (3.12 ± 0.07 mg/mL) and enhanced bioaccessibility 1.65-fold (37.31%). In vitro digestion simulations, coupled with SEM and release kinetics modeling, demonstrated the controlled release characteristics of nanoparticles in intestinal fluid. The release kinetics closely adhered to the Ritger-Peppas model, indicating Fickian diffusion behavior. This scalable, food-grade nanocarrier offers a robust strategy to enhance the solubility, bioaccessibility and targeted intestinal delivery of DMY for functional foods and nutraceuticals.
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BLOOD COAGULATION & FIBRINOLYSIS
·
2026
·
IF 1
Shen Yemei,Gao Xuemei,Huan Xuanrong,He Cui,Liu Yu,Li Yuan
Plain Language Summary This study aimed to systematically evaluate the antiplatelet activity of dihydromyricetin (DHM) and comprehensively investigate its effects on coagulation function within its therapeutically relevant concentration range. Using in-vitro experiments, we employed a panel of standard assays to assess the effects of DHM on platelet activation and aggregation induced by various agonists [adenosine diphosphate (ADP), arachidonic acid (AA), and collagen (COL)], as well as on agonist-independent adhesion and aggregation. Simultaneously, multidimensional techniques, including conventional coagulation tests, thromboelastography (TEG), and whole-blood dynamic coagulation analysis, were used to systematically evaluate the impact of DHM on the coagulation cascade and overall hemostatic dynamics. The results showed that DHM significantly inhibited platelet activation and aggregation in a concentration-dependent manner and effectively prevented their spontaneous adhesion and aggregation. Crucially, within the concentration range that exerted effective antiplatelet effects, DHM did not significantly affect any of the tested coagulation parameters, and no cytotoxicity was observed. These in-vitro findings suggest that DHM is a platelet-specific inhibitor, exerting potent antiplatelet effects while not interfering with normal physiological coagulation processes. Therefore, DHM shows potential as an antithrombotic candidate pending in-vivo studies with a potentially low risk of bleeding, providing crucial experimental evidence and a new direction for the development of safer antithrombotic strategies. Plain Language Summary This study explored the antiplatelet effects of dihydromyricetin (DHM) and its impact on blood clotting. Using lab tests, researchers found that DHM effectively reduced platelet activation and aggregation without affecting normal blood clotting or causing cell damage. DHM worked against various platelet activators like ADP, AA, and collagen, and also prevented spontaneous platelet adhesion and aggregation. Importantly, DHM did not alter coagulation parameters, suggesting it targets platelets specifically. These findings indicate DHM could be a promising antithrombotic agent with a low risk of bleeding, paving the way for safer blood clot prevention strategies in future studies. Text is machine generated and may contain inaccuracies. FAQ
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·Vol. 74
·期 35
·27621-27641
·
IF 6.7
Wu Xincheng,Lin Shiye,Zou Zebin,Huang Wenqi,Cao Hui,Xiao Jianbo
Pyrogallol-type
polyphenols undergo Strecker degradation with amino
acids in cell culture media, yet whether such chemistry measurably
alters the nutritional composition of Caenorhabditis elegans growth medium and affects worm phenotypes remains unclear. Using
a tert -butyldimethylsilyl derivatization-based GC–MS
workflow, we analyzed five flavonoids with distinct B-ring hydroxylation
patterns in the nematode growth medium (NGM): epigallocatechin gallate
(EGCG), dihydromyricetin (DHM), quercetin, luteolin, and apigenin.
Within 2 h, 400 μM EGCG and DHM reduced multiple amino acids
in NGM, with concurrent formation of the aminated derivatives N -EGCG and N -DHM identified by LC–MS/MS.
This effect persisted over 72 h in OP50-seeded NGM and propagated
to worms, lowering endogenous amino acids together with lactate and
pyruvate, and ultimately delaying growth, development, and reproductive
capacity. These findings highlight that polyphenol bioactivity studies
should not overlook chemical reactions between polyphenols and medium
constituents to avoid erroneous conclusions.
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Frontiers in Nutrition
·
2025
·
IF 5.1
Jia Li,Lijia Li,Tahui Lin,Houtan Huang,Jie Ren,Jengyuan Yao
Background: Food-derived flavonoids are emerging as nutraceutical agents for glycemic control. Dihydromyricetin (DMY), the signature flavanonol of vine tea (Ampelopsis grossedentata), has long been consumed in South China, yet its antidiabetic potential remains underexplored.Methods: We administered DMY (50 and 100 mg/kg/day, 12 days) to streptozotocin-induced type 1 diabetic mice. Fasting glycemia, lipid panels, and HOMA-β were evaluated 4 weeks after DMY administration. Untargeted UPLC-QTOF metabolomics combined with network pharmacology pinpointed pathway hubs, while experiments in INS-1 β-cells using the PI3K inhibitor LY294002 verified the pathway’s involvement.Results: DMY reduced hyperglycemia, corrected dyslipidemia, and preserved islet architecture. Metabolomics indicated a shift toward a normal plasma profile, with the arachidonic acid, linoleic acid, and steroid hormone pathways being the most responsive. Six hub targets (PTGS2, IL6, AKT1, IL1B, BCL2, CASP3) mapped to eicosanoid signaling, apoptosis, and PI3K/AKT axis. Docking and cell assays confirmed direct binding and PI3K/AKT-dependent cytoprotection, evidenced by restored p-AKT, lowered ROS, and reduced caspase-3 cleavage.Conclusion: DMY, a readily accessible food-derived bioactive compound, reprograms lipid-inflammatory metabolism and activates PI3K/AKT to safeguard β-cell viability, highlighting its nutraceutical promise for dietary management of autoimmune diabetes.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2022
·Vol. 58
·期 3
·1120-1129
·
IF 3.612
Shiyu Zhen,Sheng Geng,Hanjun Ma,Benguo Liu
Summary The non-covalent interaction between α-lactalbumin (α-La) and dihydromyricetin (DMY) was systematically revealed based on spectrofluorimetry and molecular docking method, and the potential application of their complex as an emulsifier in nano-emulsion was evaluated. The results showed that DMY and α-La could form a non-covalent complex, which led to the static fluorescence quenching of α-La, and their complexation was driven by hydrogen bonding and van der Waals force, causing the changes in the microenvironment around the tryptophan and tyrosine residues of α-La, which was consistent with molecular docking analysis. The increase of the proportion of DMY in the complex could gradually improve the ability of the complex to reduce the oil/water interfacial tension and its ABTS radical scavenging ability. The stability and β-carotene protective effect of nano-emulsion developed by the complex were superior to those of nano-emulsion stabilised by α-La, which were positively correlated with the DMY concentration in the complex.
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Food Bioscience
·
2022
·
IF 5.318
Songqi Liu,Zaihan Chen,Huange Zhang,Yuanli Li,Taxipalati Maierhaba,Jianhui An,Zhi Zhou,Lingli Deng
To explore the dihydromyricetin or eugenol loaded nanofiber's potential as food active packaging, 0%, 0.1%, 0.5%, and 1.0% dihydromyricetin or eugenol loaded nanofibers were fabricated by electro-blowing spinning, which increased the yield of nanofibers by a factor of 10 times compared to that of traditional electrospinning. The morphology observation by scanning electron microscopy showed similar morphology and diameter distribution of the dihydromyricetin or eugenol nanofibers. Fourier transform infrared spectra analysis indicated that eugenol or dihydromyricetin interacted with proteins through hydrogen bonding. X-ray diffraction profiles of the nanofibers indicated that dihydromyricetin was uniformly distributed within the nanofibers. Nanofibers encapsulated with various concentrations of eugenol or dihydromyricetin showed better barrier properties against water vapors than the free nanofibers, which indicated that the hydrophobic eugenol and dihydromyricetin inhibited the permeation of water molecules through the nanofibers. Tensile tests showed that the nanofibers loaded with 0.5% eugenol or 1.0% dihydromyricetin exhibited relatively higher elastic moduli and lower elongation at break. The nanofibers loaded with dihydromyricetin showed comparable antioxidant activity to that with eugenol in the aspect of ferric and cupper reducing activity, while that with 0.1%–1.0% dihydromyricetin showed superior DPPH radical scavenging activity. Furthermore, both eugenol and dihydromyricetin loaded gelatin/zein nanofibers showed effective antimicrobial activities against Escherichia coli and Staphylococcus aureus . Overall, the dihydromyricetin loaded gelatin/zein nanofibers showed comparable physical and functional properties compared to eugenol, which suggests the application of dihydromyricetin nanofibrous films on food active packaging and the potential application of dihydromyricetin on functional food delivery systems.
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Food & Function
·
2022
·Vol. 13
·期 9
·5061-5074
·
IF 6.317
Jie Zhao,Lin Huang,Renjie Li,Zhuangwei Zhang,Jin Chen,Hongjin Tang
Gliadin, as a main component of wheat storage protein, is used as a drug encapsulation and delivery system owing to its specific characteristics. Flavonoids are regarded as active natural products with a variety of pharmacological effects. In this study, an integrated method including UV-vis, fluorescence, and FT-IR spectroscopy and molecular modelling was applied to explore the structure–affinity relationship and the interaction nature between a library of flavonoids and gliadin. The characteristic UV-vis spectral changes of gliadin mediated by flavonoids with absorption bands at 218 and 278 nm demonstrated the existence of an interaction depending on generating the ground-state complexes. Fluorescence quenching results showed that the intrinsic fluorescence of gliadin could be effectively quenched by flavonoids coupled with the formation of flavonoid–gliadin complexes through the static quenching mechanism. The structure–affinity relationship revealed the critical structural elements associated with the binding affinity on gliadin and underlined the favorable substituents at the specific positions of flavonoid skeletons leading to a stronger binding potency. From the analysis of synchronous fluorescence spectra, flavonoids could cause the conformation change of gliadin and impact the microenvironment around TYR and TRP residues. Moreover, the ANS fluorescent probe assay suggested that these flavonoids also influenced the surface hydrophobicity of glaidin based on the further exposure or blocking of hydrophobic domains. Molecular modelling was subsequently performed and illustrated the proposed binding conformation of flavonoids on gliadin. Combined with the FT-IR spectra, these results further confirmed the important role of hydrophobic interactions and hydrogen bonds in their binding process.
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Food Chemistry-X
·
2023
·
IF 6.1
Chengyun He,Lu Bai,Daqun Liu,Benguo Liu
The binding capacity of 10 flavonoids with okra seed protein (OSP) was studied by fluorescence spectroscopy. The structure of flavonoids had an obvious impact on binding performance. The binding ability of flavanone was lower than that of flavone, isoflavone and dihydrochalcone. The binding capacity of flavonoid glycoside was superior to that of the corresponding flavonoid aglycone. The binding ability was positively correlated with the number of phenolic hydroxyl groups on the B ring. The steric field and electrostatic field model constructed by 3D-QSAR method could well explain the above interaction behavior. Thermodynamic analysis suggested that the quenching mechanism of OSP caused by flavonoids was static quenching, and the binding-site number was 1. In addition, hydrogen bonding and van der Waals force dominated this interaction. The 3D and synchronous fluorescence spectra showed that there was no significant change in the polarity of the environment around tryptophan and tyrosine residues during binding.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Dongqing Zhang,Wenqi Liu,Ke Ye,Xiaojin Li
Proton exchange membrane fuel cells (PEMFCs) are hindered by their poor tolerance to CO and H 2 S poisoning. Herein, we report an effective method, via engineering 2,6-dihydroxymethyl pyridine (DhmPy) molecule blocking layers on Pt surface, aiming to save the poisoning issue for PEMFC anode reaction. The PEMFCs assembled by this catalyst produce a power density of 1.18 W cm −2 @ 2.0 A cm −2 and 1.32 W cm −2 @ 2.0 A cm −2 , far exceeding commercial Pt/C after H 2 /10 ppm CO poisoning and H 2 /5 ppm H 2 S poisoning tests, respectively. Density functional theory (DFT) indicates that a coronal molecule layer with a steric confinement height (1.82 Å), constructed by DhmPy, emerges more intensive adsorption energy compared to 2,6-pyridinedicarboxamide (DcaPy) and 2,6-diacetylpyridine (DAcPy), thereby more effectively inhibits the adsorption of large-sized CO and H 2 S on Pt surface without affecting H 2 traverse. This “work along both lines” mechanism with the resistance of both CO and H 2 S provides a new and promising design thought for high CO and sulfur tolerant PEMFC anodes.
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