ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Yi Zeng,Xin Du,Bingbing Gao,Bing Liu,Zhuoying Xie,Zhongze Gu
The combination of surface-enhanced Raman scattering (SERS) with high-throughput screening (HTS) has significant importance for highly sensitive and massive workload assays. Although fabrication of HTS–SERS substrates can be achieved by several methods, the high cost as well as large-equipment dependence limit their applications. Here, we report a simple method to fabricate HTS–SERS substrates within one-step process. The HTS–SERS substrate is fabricated by simply UV-irradiating a fluoroalkylsilane (FAS)-modified liquid-repellent TiO2 surface in AgNO3 solution through a photomask. Owing to the photocatalytic nature of TiO2, the UV irradiation simultaneously triggers the degradation of the attached FAS and the generation of liquid-adhesive Ag nanoparticles (NPs) on exposed area. A HTS–SERS substrate could be directly obtained after UV irradiation. The deposited Ag NPs evidently enhance Raman signals, and the significant difference between the wettability of exposed area and masked area enables fast formation of high-throughput liquid droplet arrays through a simple dragging solution process. The fabrication method is applicable to various substrate materials, to introduce additional functionalities. The photocatalytic activity of TiO2 also allows us to photobleach the residual analyte and Ag NPs after detection to recycle substrate. This single-step method is a highly promising candidate for the fabrication of HTS–SERS substrates.
研究领域:
分析化学
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材料科学
·
纳米技术
·
光学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
·
2017
·Vol. 184
·期 1
·60-74
·
IF 2.399
Tao Ran,Wang Chengzhang,Zhang Changwei,Li WenJun,Zhou Hao,Chen Hongxia,Ye Jianzhong
The structure and bioactivity of Ginkgo biloba leaves polyprenol (GBP) are similar to that of dolichol which widely exists in human and mammalian organs. GBP possesses potential pharmacological activities against cancer. This study involved oil-in-water type nanoemulsion (NE) loading GBP was prepared by dissolving polyprenol in nanoemulsion of sodium tripolyphosphate (TPP)/TiO 2 solution, Triton X-100, and 1-octanol by inversed-phase emulsification (EIP) and ultrasonic emulsification (UE) method. Folic acid (FA)-coupled chitosan (CS) nanoparticles (NPs), GBP-FA-CS-NPs and GBP-TiO 2 -FA-CS-NPs, were fabricated by ionic cross-linking of positively charged FA-CS conjugates and negatively charged nanoemulsion with TPP/TiO 2 . And characterizations of them were investigated by TEM, SEM, FTIR, particle size, and zeta potential. The cytotoxic and genotoxic effects of GBP-TiO 2 -FA-CS-NP treatment were higher than GBP-NE, GBP-FA-CS-NPs, TiO 2 -NE, GBP-TiO 2 -NE, TiO 2 -FA-CS-NPs, and GBP-TiO 2 -FA-CS-NP treatment at the same tested concentrations in HepG2 cells. GBP-TiO 2 -FA-CS-NPs at low TiO 2 concentration (from 1 to 2.5 μg/ml) showed good inhibition capacity on HepG2 cells and low cytotoxic and genotoxic effects on HL-7702 cells. The possible mechanism of cytotoxicity on GBP-TiO 2 -FA-CS-NPs against HepG2 cells is by preventing excessive intracellular Ca 2+ into extracellular spaces via inhibiting Ca 2+ -ATPase and Ca 2+ /Mg 2+ -ATPase.
研究领域:
药理学
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纳米技术
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毒理学
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癌症研究
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2021
·
IF 5.161
Fei Sun,Ting-Ting Li,Hai-Tao Ren,Bing-Chiuan Shiu,Hao-Kai Peng,Jia-Horng Lin,Ching-Wen Lou
Increasing oily wastewater is produced by various industrial, various oil-water separation technologies and materials have been developed to solve this issue. A facile method is proposed to build multi-scaled and hierarchical membranes with efficiently oil/water separation and photocatalysis. The multi-scaled, hierarchical membrane is fabricated by a hydrophilic polyacrylonitrile (PAN) electrospinning nanofibrous and PAN/TiO 2 electrospraying spheres, then the polydopamine (PDA) decorated membrane via in-situ growth. This multi-scaled, hierarchical structure endows the membrane with superhydrophilicity (water contact angle ∼ 0°), and underwater superoleophobicity (oil contact angle ∼152°). And the multi-scaled, hierarchical membrane shows superior water permeation and hydrophilic properties than pure PAN membranes. In addition, the membrane indicates an excellent photocatalytic ability for organic pollutant (∼98 %). And after 5 cycles, the water flux still maintains a high level showing good recyclability. This multi-scaled, hierarchical nanofibrous membrane shows very promising application potentials in oil-water separation and photocatalysis.
研究领域:
环境科学
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材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 50
·2106249
·
IF 18.808
Xiao Huang,Jun Xing,Zhengao Wang,Jin Han,Renxian Wang,Changhao Li,Cairong Xiao,Fang Lu,Jinxia Zhai,Zhengnan Zhou,Yangfan Li,Lei Zhou,Zhiguo Song,Dafu Chen,Peng Yu,Chengyun Ning,Xieyuan Jiang
Mimicking the natural bone extracellular matrix containing intrinsic topography and electrical signals is an effective way to modulate bone regeneration. However, simultaneously coupling of the intrinsic mechanobiology and electrical cues of implant to modulate bone regeneration remains ignored. Here, the authors report in situ designation of titanium dioxide (TiO 2 ) nanocone/bismuth oxide (Bi 2 O 3 ) nanodot heterojunctions on bone implant surface to electro-biomechanically trigger osseointegration at bone/implant interface. TiO 2 nanocone/Bi 2 O 3 nanodot heterojunctions exhibit built-in electric field at the nanoscale interface and elastic modulus equivalent to that of bone tissue. The nano-heterojunctions significantly promoted the attachment, spreading, and osteogenic differentiation of bone marrow mesenchymal stem cells in vitro, and the osteogenesis in vivo. The authors also show that the effects of nano-heterojunctions on osteogenesis are mediated by yes-associated protein biomechanical signal pathway and intracellular enrichment induced Phosphatidylinositol 3-kinase signal pathway. Their findings highlight the coupling of topographical and electric parameters of biomaterials for modulating cell behaviors.
研究领域:
材料科学
·
组织工程
·
再生医学
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细胞生物学
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生物医学工程
✓ 已验证
DOI
高影响因子
Nano Research
·
2023
·Vol. 16
·期 5
·7280-7292
·
IF 9.9
Sun Yue,Wang Yihui,Han Ruyin,Ren Ziwei,Chen Xin,Dong Wenzhuo,Choi Stephen,Liu Quanhong,Wang Xiaobing
Sonodynamic therapy (SDT) has attracted great interest in the field of cancer therapy because of its non-invasiveness, deep penetration, and spatiotemporal controllability. However, the sonodynamic effect is severely hindered by hypoxia and a high glutathione (GSH) level in tumor microenvironment. In this work, a new type of nanohybrid sonosensitizer is designed, which incorporates several prominent advantages of organic, inorganic, and natural sonosensitizers for imaging-guided SDT and ferroptosis induction. As an endogenous transporter, hemoglobin (Hb) has dual functions of oxygen and iron supplement. Apparently, Hb can transport iron to the tumor site for Fe-dependent ferroptosis, yet this oxygen carrier is able to enhance the sensitivity of tumor cells to oxygen-driven SDT. We innovatively hybridized Hb, sinoporphyrin sodium (DVDMS), and titanium dioxide (TiO 2 ) to prepare an integrated nano-oxygen carrier (designated as HDT) for multifarious cancer theranostics, which not only attained satisfactory sonosensitization of DVDMS/TiO 2 but also achieved oxygen-boosted SDT and potent ferroptosis via Hb. Under ultrasound irradiation, the oxygenation of HDT markedly amplified the generation of reactive oxygen species (ROS) in hypoxic tumors, promoted the valence transition of Hb to accelerate Fenton reaction, and led to ferroptosis. This strategy virtually eradicated tumors in situ and exhibited immunoregulatory potential for augmenting anti-tumor effects in vivo and in vitro through ROS formation, O 2 self-supplement, GSH depletion, and lipid peroxidation. Collectively, HDT demonstrated excellent dual-modal imaging performance, providing a valuable platform for photoacoustic/fluorescence-guided SDT and ferroptosis induction in tumor cells.
研究领域:
癌症治疗
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纳米技术
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生物医学工程
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分子生物学
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成像技术
✓ 已验证
产品关联
DOI
高影响因子
Acta Pharmaceutica Sinica B
·
2022
·
IF 14.903
Zekun Li,Yongchun Pan,Shiyu Du,Yayao Li,Chao Chen,Hongxiu Song,Yueyao Wu,Xiaowei Luan,Qin Xu,Xiaoxiang Guan,Yujun Song,Xin Han
Near-infrared (NIR)-light-triggered nanomedicine, including photodynamic therapy (PDT) and photothermal therapy (PTT), is growing an attractive approach for cancer therapy due to its high spatiotemporal controllability and minimal invasion, but the tumor eradication is limited by the intrinsic anti-stress response of tumor cells. Herein, we fabricate a tumor-microenvironment responsive CRISPR nanoplatform based on oxygen-deficient titania (TiO 2- x ) for mild NIR-phototherapy. In tumor microenvironment, the overexpressed hyaluronidase (HAase) and glutathione (GSH) can readily destroy hyaluronic acid (HA) and disulfide bond and releases the Cas9/sgRNA from TiO 2- x to target the stress alleviating regulators, i.e. , nuclear factor E2-related factor 2 (NRF2) and heat shock protein 90 α (HSP90 α ), thereby reducing the stress tolerance of tumor cells. Under subsequent NIR light illumination, the TiO 2- x demonstrates a higher anticancer effect both in vitro and in vivo . This strategy not only provides a promising modality to kills cancer cells in a minimal side-effects manner by interrupting anti-stress pathways but also proposes a general approach to achieve controllable gene editing in tumor region without unwanted genetic mutation in normal environments.
研究领域:
癌症治疗
·
纳米技术
·
基因编辑
·
光疗
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioactive Materials
·
2024
·
IF 18
Shunlai Shang,Kaiting Zhuang,Jianwen Chen,Ming Zhang,Shimin Jiang,Wenge Li
Mesenchymal stem cell derived exosomes (MSC-Exos) demonstrate beneficial effects on wound healing via anti-inflammatory and angiogenic properties. Chitosan (CS) exhibits excellent biocompatibility and accelerates cellular migration, adhesion, and proliferation. The ions released from bioactive glass (BG) and titanium dioxide (TiO 2 ) nanoparticles exhibit sustained angiogenic and antibacterial potency. In this study, CMCS-CEBT hydrogel was synthesized from exosomes encapsulated carboxymethyl chitosan (CMCS), chitosan nanoparticles (CS-NPs), BG, and TiO 2 nanoparticles for a preliminary evaluation of its impacts on the treatment of full-thickness skin defects, diabetic wounds, and burn skin injury due to burns. In vitro analysis indicated that the hydrogel exhibits excellent cell compatibility, stimulates endothelial cell adhesion and proliferation, and presents anti-inflammatory, angiogenic, and antibacterial activities. In vivo, the composite hydrogel dressing accelerated a wound healing acceleration effect, stimulated angiogenesis, and increased collagen deposition and the expression of anti-inflammatory factors. This innovative composite hydrogel dressing as a potential clinical therapy, utilizing bioactive materials , holds promise as a potential clinical therapy that aims to facilitate the regeneration of acute and chronically damaged skin tissue.
研究领域:
生物医学工程
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组织工程
·
伤口愈合
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纳米技术
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Zulan Li,Ye Liu,Xingming Chen,Hongyan Cao,Haiying Shen,Lei Mou,Xinli Deng,Xingyu Jiang,Yulong Cong
How to balance the sensitivity and signal-to-noise ratio of immunosensor remains many challenges during various diseases diagnosis. Here we develop a new microfluidic immunosensor based on surface-modified mesoporous nanofibers , and simultaneously realize an ultra-sensitivity and high signal-to-noise ratio for the detection of multiple biomarkers. In the current study, we fabricated titanium dioxide (TiO 2 )-based mesoporous electrospinning nanofibers, and modified nanofiber surface with both octadecylphosphonic acid (OPA) and poly(ethylene oxide)-poly(propylene oxide) triblock copolymer (PEO-PPO-PEO). Such nanofibers as solid substrate are covered on microfluidic channels. The porosity of our nanofibers dramatically increased the adsorption capability of antibodies, realizing an ultra sensitivity of biomarker detection. PEO-PPO-PEO modification can significantly block non-specific absorptions, obtaining a satisfied signal-to-noise ratio. For the detection of HIV p24 and interleukin 5 (IL-5), our immunosensor increased 6.41 and 6.93 fold in sensitivity and improved 504.66% and 512.80% in signal-to-noise ratio, in compared with gold standard immunoassay (ELISA) used in the clinic. Our immunosensor also broaden the linear range for the detection of HIV p24 (0.86–800 pg/ml) and IL-5 (0.70–800 pg/ml), in compared with ELISA which is 5.54–500 pg/ml for HIV p24 and 4.84–500 pg/ml for IL-5. Our work provided a guideline for the construction of advanced point-of-care immunosensor with an ultra-sensitivity and high signal-to-noise ratio for disease diagnosis.
研究领域:
生物传感器
·
纳米技术
·
疾病诊断
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2023
·
IF 8.4
Yiguo Shen,Kun Wang,Weiliang Wu,Wei Feng,Jiansong Chen,Qiang Gao
Postoperative bacterial infection and poor osseointegration with surrounding bone tissue are not only the critical causes of implant failure but also the major challenges in clinical application. Hereby, Titania (TiO 2 ) microporous/nanofiber coating was firstly prepared on the surface of titanium (Ti) material by alkali-heat treatment process, and strontium (Sr)-doped coating (TS) was then prepared by hydrothermal synthesis. Finally, polydopamine-assisted co-deposition technology was utilized to deposit vancomycin (Van) onto TS surface (TSV), thus obtaining a medical composite coating with antibacterial property and improved osseointegration ability. According to antibacterial test, TSV has not only a 99.99% killing effect on methicillin-resistant Staphylococcus aureus (MRSA), but also a good anti-biofilm ability. The cell experiments showed that TSV could enhance the adhesion and proliferation of osteoblasts. Compared with pure Ti and TS, TSV induces the formation of bone apatite in simulated body fluid (SBF) test quickly, indicating that the coating has good biological activity. According to histopathological observation and bone morphology analysis, TSV showed highly effective infection prevention and excellent osteointegration in vivo . These test results suggested that it is feasible to prevent implant-associated infection and promote osseointegration by functionalization of the implant, so as to provide a fresh direction for clinical research.
研究领域:
生物材料
·
生物医学工程
·
骨科
·
传染病学
·
组织工程
✓ 已验证
产品关联
DOI
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2022
·
IF 6.206
Xueya Liu,Yingqin Wei,Furong Tao,Xue Zhang,Ligang Gai,Libin Liu
Superhydrophobic surfaces with reversible switchable wettability have great application value in the treatment of oily wastewater. However, the advancement of switchable wettability surfaces is limited by the complexity of the manufacturing process and the excessive use of environmentally unfriendly solvents. Herein, an all-water-based reversible wettability coating was fabricated by simply spraying a water suspension consisting of N-[3-(trimethoxysilyl) propyl] ethylenediamine (AS)-, 3-(trimethoxysilyl) propyl methacrylate (KH-570)-, and 1H,1H,2H,2H-perfluorodecyltrimethoxysilane (FAS)-modified titanium dioxide (TiO 2 ) nanoparticles and cellulose . The coating possesses good adhesion with various substrates and exhibits stable mechanical and chemical stability as well as good resistance to harsh environments such as various organic solvents , strongly corrosive environments and extreme temperature environments. The coating can be switched between having superhydrophobicity (160°) and superhydrophilicity under UV irradiation and heating treatment. The coated cotton fabric can separate heavy/light oil-water mixtures and water-in-oil/oil-in-water emulsions with a high efficiency of greater than 95%. Due to the photocatalytic properties of TiO 2 , the coating can degrade soluble pollutants in water during UV irradiation, and the degradation efficiency is over 97%. This study provides a green industrial route for designing a simple, switchable wettability coating, which is expected to promote the large-scale industrial production and application of functional smart surfaces.
研究领域:
材料科学
·
环境科学
·
化学工程
·
表面化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）