FOOD CHEMISTRY
·
2015
·
IF 3.391
Juying Hou,Guangjuan Dong,Zhengbin Tian,Jiutian Lu,Qianqian Wang,Shiyun Ai,Minglin Wang
In this paper, a simple and sensitive fluorescent sensor for dichlorvos was first constructed based on carbon dots-Cu(II) system. These carbon dots were obtained by simple hydrothermal reaction of feather. The fluorescence of these carbon dots can be selectively quenched by Cu 2+ ion. When acetylcholinesterase and acetylthiocholine were introduced into the system, thiocholine came into being, which can react with Cu 2+ ion and restore the fluorescence of the system. The reaction mechanism between Cu 2+ ion and thiocholine was confirmed by X-ray photoelectron spectroscopy. As one kind of acetylcholinesterase inhibitor, organophosphorus pesticides can be detected based on this sensing system. As an example of organophosphorus pesticides, dichlorvos was detected with a linear range of 6.0 × 10 −9 –6.0 × 10 −8 M. This sensing system has been successfully used for the analysis of cabbage and fruit juice samples.
研究领域:
分析化学
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环境科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Bao-Zhu Jia,Yan-Yan Liu,Feng-Yan Chen,Phannika Tongchai,Sumed Yadoung,Anurak Wongta,Zhen-Lin Xu,Lin Luo,Surat Hongsibsong
Dichlorvos (DDVP), a widely used yet environmentally hazardous organophosphate pesticide, necessitates rapid field monitoring due to increasing regulatory restrictions. In this study, we firstly present a gold-nanoparticle-based lateral flow immunochromatographic assay (LFIA) employing a highly specific anti-DDVP monoclonal antibody for onsite DDVP detection within 20 min. The optimized LFIA exhibited exceptional analytical performance, with limits of detection spanning 16 μg/kg to 108 μg/kg across six fruit and vegetable matrices—all below the established maximum residue limits in foods. Method validation with spiked fruit and vegetable samples showed recovery rates of 87.3–109.4 % and 89.2–103.3 %, respectively, with coefficients of variation below 8.3 % and 7.9 %, respectively, confirming the accuracy and precision of the assay. Notably, LFIA exhibited good agreement with standard GC–MS/MS analysis when applied to 144 authentic samples, further validating its reliability for real-world applications. This LFIA offers key advantages including portability, cost-effectiveness, and compatibility with regulatory thresholds, making it an ideal alternative to conventional instrumental methods for routine pesticide residue screening in food safety supervision settings.
研究领域:
食品安全
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分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
He Mei,Xiaolei Zhu,Ziqian Li,Jiahui Jiang,Huili Wang,Xuedong Wang,Peipei Zhou
Herein, we developed a ratiometric fluoroprobe by integrating okra-derived carbon quantum dots (CQDs) with amplex red (AR) using manganese dioxide nanosheets (MnO 2 NSs) as a medium. Fluorescence intensities (FIs) of CQDs were sharply quenched by MnO 2 NSs via an inner-filter effect processes, whereas the FIs of AR were significantly enhanced due to oxidation of AR to AR-ox by the oxidase-mimetic activity of MnO 2 NSs. Acetyrylcholinesterase hydrolyzed acetylthiocholine to produce thiocholine, and the decomposition of MnO 2 NSs to Mn 2+ by thiocholine led to the FI recovery of CQDs, but decreased FIs of AR-ox. Based on the above phenomenon and the inhibitory effect of dichlorvos (DDVP) on acetyrylcholinesterase activity, a novel ratiometric fluoroprobe for DDVP quantification was pioneered. Under optimized conditions, this fluoroprobe gave a wide linear range (4–120 μg/L), low detection limit (1.2 μg/L), and satisfactory fortification recoveries (90.0–110.0%), thereby providing good prospects for routine DDVP monitoring in foods.
研究领域:
分析化学
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食品安全
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生物传感
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Jianhua Zhang,Huaying Hu,Lianqiao Yang
A highly stable acetylcholinesterase (AChE) sensor based on titanium oxide nanorods (TiO 2 -NRs) and reduced graphene oxide (rGO) for detecting dichlorvos (DDVP) is successfully developed. The sensor adopts a layer-by-layer assembly method to sequentially assemble chitosan (CS)-modified reduced graphene oxide (denoted CS&rGO), gold nanoparticles (Au-NPs), TiO 2 -NRs, and acetylcholinesterase (AChE) on the surface of a glassy carbon electrode (GCE). The catalytic activity, impedance characteristics, material effects, the relationship between the inhibition rate of the sensor and the dichlorvos (DDVP) concentration, interference studies, real sample detection, and storage stability of the sensor were all evaluated using electrochemical characterization. The sensor’s response to DDVP ranged from 2.26 nM to 565 nM, with a limit of detection of 2.23 nM, and it displayed good repeatability, selectivity, and storage stability.
研究领域:
生物化学
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Juying Hou,Zhengbin Tian,Huizhi Xie,Qingyuan Tian,Shiyun Ai
Here we first reported an ultrasensitive fluorescence resonance energy transfer (FRET) biosensor of dichlorvos based on carbon dots and the traditionally colorimetric Ellman’s test. In Ellman’s test, the absorption intensity of 5-thio-2-nitrobenzoic acid anion (TNB − ) at 412 nm can be used for the determination of acetylcholinesterase (AChE) and its inhibitor. We chose TNB − as energy acceptors to construct a FRET sensing system of AChE and its inhibitor. Quaternized carbon dots (Q-CDs) were chosen as energy donors to form an effective FRET system. By a two-step synthetic method, the Q-CDs with high quantum yield (34.2%) were successfully prepared. Experimental results showed TNB − can quench the fluorescence of Q-CDs to a large extent, which indicated an effective FRET between Q-CDs and TNB − was successfully established. As the inhibitor of AChE and common organophosphorus insecticide, dichlorvos can be detected using this sensing system. Under optimal conditions, the linear range of dichlorvos was 5.0 × 10 −11 –1.0 × 10 −7 M. Owing to the high sensitivity and green fluorescent material with high quantum yield, this FRET sensing system was adaptable for automated analyzers using in the rapid determination of a great quantity of samples.
研究领域:
分析化学
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环境科学
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毒理学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 26
·11336-11341
·
IF 3.288
Dongying Shi,Chao-Jie Cui,Chun-Xiao Sun,Jun-Ping Du,Chun-Sen Liu
A new [Co21(H2O)4(OH)12]30+ unit-incorporating polyoxotungstate, Na18[Co(H2O)6]2{[Na(H2O)4]2[Na(H2O)2]2[Co(H2O)3]2[Co21(H2O)4(OH)12](SiW10O37)6}·24H2O (denoted as Co25Si6W60), has been hydrothermally synthesized and fully characterized via elemental analyses, thermogravimetric analysis, bond valence sum (BVS) calculations, IR spectrum, UV-vis spectrum, diffuse reflectance spectrum, powder X-ray diffraction and single-crystal structural analysis. The most remarkable feature is that the gigantic [Co21(H2O)4(OH)12(SiW10O37)6]30− core in Co25Si6W60 is composed of a square-like [Co21(H2O)4(OH)12]30+ nanosheet encapsulated by six dilacunary Keggin [α-SiW10O37]10− moieties in a staggered fashion, resulting in a remarkable sandwich-type assembly. Furthermore, the unique Co25Si6W60-based biosensing system, as a simple, rapid, and highly sensitive electrochemical platform, offers an environmentally friendly route for detecting dichlorvos in the field of both food safety and environmental pollution.
研究领域:
无机化学
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分析化学
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环境科学
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材料科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 8
·1-12
·
IF 6.408
Wan Yu,Wang Huiting,Zhang Ling,Chen Yaxian,Li Shun,Zhou Jie,Zhang Qian,Xia Lixin
A highly stable electrochemical biosensor for pesticide detection was developed. For the first time polymeric ionic liquids (PILs) were introduced to construct an acetylcholinesterase (AChE) biosensor . AChE was entrapped in PILs microspheres through an emulsion polymerization reaction, where negatively charged Au nanoparticles (Au NPs) can be immobilized by the positively charged PILs, leading to improved catalytic performance. The results suggest that the positively charged PILs not only provide a biocompatible microenvironment around the enzyme molecule, stabilizing its biological activity and preventing its leakage, but also act as a modifiable interface allowing other components with electron transport properties to be loaded onto the polymer substrate, thus providing an efficient electron transport channel for the entrapped enzyme. More notably, when AChE was immobilized in a positively charged environment, the active site is closer to the electrode, promoting faster electron transfer. The detection limits of the constructed electrochemical biosensor AChE@PILs@Au NPs/GCE toward carbaryl and dichlorvos (DDVP) were 5.0 × 10 −2 ng ml −1 and 3.9 × 10 −2 ng ml −1 , in a wide linear range of 6.3 × 10 −2 –8.8 × 10 2 ng ml −1 and 1.3 × 10 −1 –1.4 × 10 3 ng ml −1 , respectively. More importantly, the biosensor has high thermal and storage stability, which facilitates rapid field analysis of fruits and vegetables in a variety of climates. In addition, the biosensor reported has good repeatability and selectivity and has high accuracy in the analysis of peaches, tap water, and other types of samples. Graphical abstract
研究领域:
分析化学
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环境科学
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材料科学
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生物化学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 39
·3911-3920
·
IF 3.532
Limin Yang,Xiaolong Zhang,Mingming Li,Linjiao Qu,Zhen Liu
Immobilization of enzymes is one of the key steps in the development of high-performance enzymatic electrochemical biosensors, and various nanostructured materials have been designed and developed to achieve this goal. Herein, hybrid nanoflowers (HNFs) were synthesized using acetylcholinesterase (AChE) as an organic component and copper phosphate (Cu3(PO4)2) as an inorganic component. These AChE–Cu3(PO4)2 HNFs exhibit a three-dimensional hierarchical flower-like structure, which not only has a large specific surface area but also promotes the affinity between AChE and its substrate with better catalytic activity. Not only that, the surface modification of the glassy carbon electrode (GCE) by the joint use of gold nanoparticles (AuNPs) and graphene oxide (GO) extended the electroactive area. Using square-wave voltammetry (SWV), the as-prepared biosensor (i.e., AChE–Cu3(PO4)2 HNF/AuNP/GO/GCE) demonstrated superior sensing performance in the detection of dichlorvos. The detection limit is as low as 0.07 pM, and the linear detection range can range from 0.5 pM to 10 μM. In addition, the biosensor was feasible in real agricultural samples with satisfactory recoveries (98.65% to 103.43%). The reported biosensor provides an alternative tool for the direct measurements of AChE activity and its inhibition. Besides organophosphorus pesticides represented by dichlorvos, this biosensor has the potential to detect other AChE inhibitors, such as carbamate pesticides, drugs for Alzheimer's disease, etc., thus having broader applications in food safety and drug screening.
研究领域:
生物化学
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分析化学
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纳米技术
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环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Fei Tong,Zan Yang,Zheng Wang,Wenya Liu,Wanqi Jiang,Lu Zhu,Lei Wang,Mingming Zheng,Ruyan Hou,Yibin Zhou,Yingnan Liu
In view of the potential hazards of organophosphorus pesticides (OPs), this paper constructed a ratiometric fluorescent probe utilizing a functionalized metal–organic framework to detect OPs. Ru(bpy) 3 Cl 2 was encapsulated inside UiO-66 as a reference signal, and MnO 2 nanosheets (MnO 2 NSs) were grown on the surface to obtain [email protected] @MnO 2 NSs. Acetylcholinesterase catalyzed the decomposition of acetylcholine into reductive thiocholine, which consumed MnO 2 NSs, thus restoring the [email protected] fluorescence. Due to the enzymatic inhibition of OPs and the redox reaction between MnO 2 NSs and thiamine, this probe emitted blue fluorescence in the presence of OPs. The probe achieved linear responses to dichlorvos and chlorpyrifos with LODs of 9.99 × 10 -6 μg mL −1 and 9.99 × 10 -5 μg mL −1 . The probe exhibited a satisfactory recovery rate for OPs in green tea. Furthermore, a hydrogel detection platform was developed by embedding the probe into sodium alginate. Overall, this work provides a visual approach to detect OPs in agricultural products.
研究领域:
分析化学
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环境科学
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材料科学
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农业科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Shi Gang Liu,Haoyu Wang,Qian Zhao,Wenli Gao,Xingbo Shi,Zhonghua Liu
Background Dichlorvos (DDVP), as a highly effective insecticide, is widely used in agricultural production. However, DDVP residue in foodstuffs adversely affects human health. Conventional instrumental analysis can provide highly sensitive and accurate detection of DDVP, while the need of bulky and expensive equipment limits their application in resource-poor areas and on-site detection. Therefore, the development of easily portable sensing platforms for convenient, rapid and sensitive quantification of DDVP is very essential for ensuring food safety. Result A portable colorimetric sensing platform for rapid and sensitive quantification of DDVP is developed based on nanozyme-participated highly efficient chromogenic catalysis. The Fe-Mn bimetallic oxide (FeMnOx) nanozyme possesses excellently oxidase-like activity and can efficiently catalyze oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) into a blue oxide with a very low Michaelis constant (K m ) of 0.0522 mM. The nanozyme-catalyzed chromogenic reaction can be mediated by DDVP via inhibiting the acetylcholinesterase (AChE) activity. Thus, trace DDVP concentration-dependent color evolution is achieved and DDVP can be sensitively detected by spectrophotometry . Furthermore, a smartphone-integrated 3D-printed miniature lightbox is fabricated as the colorimetric signal acquisition and processing device. Based on the FeMnOx nanozyme and smartphone-integrated lightbox system, the portable colorimetric sensing platform of DDVP is obtained and it has a wide linear range from 1 to 3000 ng mL −1 with a low limit of detection (LOD) of 0.267 ng mL −1 for DDVP quantification. Significance This represents a new portable colorimetric sensing platform that can perform detection of DDVP in foodstuffs with simplicity, sensitivity, and low cost. The work not only offers an alternative to rapid and sensitive detection of DDVP, but also provides a new insight for the development of advanced sensors by the combination of nanozyme, 3D-printing and information technologies.
研究领域:
分析化学
·
纳米技术
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食品安全
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环境科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）