JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.2
Meina Jin,Xiaoyue Lun,Wei Mu,Yunhe Zhao,Zhengqun Zhang
The insect olfactory system serves as a critical detection mechanism, where odorant-binding proteins (OBPs) mediate recognition by binding odor molecules, thereby regulating behavioral responses. Here, we functionally characterized three male antennal-enriched OBPs (BodoOBP2, BodoOBP4, and BodoOBP8) from Bradysia odoriphaga. Fluorescence binding assays showed that these OBPs had significantly higher affinity for three sex pheromones than for five host plant volatiles, with BodoOBP8’s affinity for linolenic acid particularly outstanding (Ki = 0.44 μΜ, pH = 5.0). Molecular docking attributed these profiles to key pocket residues. RNA interference showed that dsRNA-treated males had significantly impaired female recognition. Furthermore, host-plant attraction assays showed a reduced Chinese chive orientation in dsBodoOBP2- and dsBodoOBP4-silenced adults. Our findings suggest that BodoOBP2 and BodoOBP4 mediate both pheromone communication and host-plant detection, while BodoOBP8 mainly transports pheromones. These results advance our understanding of olfactory mechanisms in Diptera and provide molecular targets for olfactory-based management of this pest.
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OPTICS LETTERS
·
2026
·Vol. 51
·期 4
·857-860
·
IF 3.3
Feng Liu,Feng Liu,Feng Liu,Zixuan Huang,Zixuan Huang,Jiao Li,Liming Li,Sheng Liu,Peng Li,Xiaowei Lei,Jiwei Zhang,Biqiang Jiang,Xuetao Gan,Jianlin Zhao
Chalcogenide polymeric materials have emerged as promising, cost-effective alternatives to conventional infrared materials. In this Letter, we demonstrate the fabrication of micro-optics elements using a flexible chalcogenide polymer with an extended transparency window into the long-wave infrared region. Employing this thermoset S-DADS polymer, broadband infrared micro-optical components were fabricated via casting processes. Experimental results validate their functionality in IR imaging, wavefront sensing, and mechanically tunable beam shaping, which shows their potential for diverse infrared applications.
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CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Ting Wang,Jing Deng,Ruijin Ran,Wenqiang Shi,Yunxia Gao,Xiang Ren,Jun Cao,Ming Zhang
Endotamponade is in huge demand for vitrectomy, while current vitreous substitutes exhibit instability and foreign body reactions that impede long-term implantation. Herein, we report an in-situ forming hydrogel (PEGBAA) with prominent stability and anti-fouling properties as a long-term vitreous substitute consisting of two main components: functional-modified multi-arm poly(ethylene glycol) (8sPEG-SH/Mal) and zwitterionic poly(carboxybetaine) (pCBAA). The former is bioinert to provide long-term stability via the thiol-ene Michael addition reaction. The latter possesses anti-fouling characteristics to prevent adsorption of protein and cells due to its strong hydrophilicity. The PEGBAA hydrogel formed fast in situ exhibits reduced protein adsorption and cell adhesion compared to pure PEG-based hydrogels, good in vitro biocompatibility, and very similar critical parameters (i.e., light transmittance , refractive index , water content, modulus, high stability) to the natural vitreous humor. After a 180-day implantation through injection with a double syringe via standard vitrectomy procedures in rabbit models, the hydrogels acts as a very stable vitreous substitute without abnormal complications. Therefore, the strategy of in-situ forming hydrogels with anti-fouling properties offers a new avenue to improve the in vivo stability of long-term vitreous endotamponades.
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JOURNAL OF FOOD SCIENCE
·
2020
·Vol. 85
·期 10
·3562-3569
·
IF 2.479
Tingting Li,Likun Ren,Dangfeng Wang,Minjie Song,Qiuying Li,Jianrong Li
Low-purine food is not only the focus of gout patients, but also the focus of contemporary green diet development. Fish are usually considered as high-purine foodstuff because of the high nutritional value and high purine content. Therefore, it is necessary to reduce purine content in fish to ensure that they are suitable for patients with hyperuricemia or gout. In this study, the effect of allicin on purine reduction in turbot during cooking was investigated using high-performance liquid chromatography, and the change of xanthine oxidase (XO) activity was also studied. Molecular docking analysis was further performed to elucidate the mechanism of purine reduction by allicin. The results revealed that in the step of soaking, allicin could reduce purine content in fish by slightly enhancing XO activity, promoting hypoxanthine transformation into xanthine. The removal of total purines in experimental and control group reached 70.45% and 57.20%, respectively. Moreover, allicin could change the thermal stability of xanthine by providing an acidic environment, resulting in the rapid decrease of xanthine and hypoxanthine levels by boiling. Thus, this study provides a simple method to decrease purine levels, suggesting a possibility that allicin can function as a purine remover in food.
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JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2020
·Vol. 58
·期 3
·1061-1071
·
IF 1.946
Zhang Bin,Zheng Zhenjia,Liu Nan,Liu Pengli,Qiu Zhichang,Qiao Xuguang
The aim of this study was to investigate the effects of the sequence of different thermal and mechanical treatments on the physicochemical parameters and microstructure of garlic paste, in order to improve the quality of the product. The total organosulfur compounds (OSCs) decreased steadily in blended-heated garlic, whereas OSCs decreased sharply after 2 min at 75 °C or 5 min at 85 and 95 °C in heated-blended garlic. After blanching for 5 min, allicin could maintain over 4.0 mg/g only at 75 °C; and OSCs of heated-blended garlic paste were found to drop by 29.56%, 90.63% and 94.79% at 75, 85 and 95 °C, respectively. In blended-heated garlic, the color values of L * (lightness) and a * (redness) decreased ( P < 0.05), while the b * (yellowness) and C * (chroma) increased ( P < 0.05), obtaining green discoloration garlic paste. The total color differences of blended-heated samples were greater than 12.08, which were 2–6 folds higher compared with heated-blended garlic. Total phenolic content and antioxidant activity decreased ( P < 0.05) in all thermal treatments, thermal treatment of heated-blended garlic less than 5 min maintained over 30% of antiradical activity. The sequence of unit operations determined the pattern of garlic microstructure disruption, resulting in various enzymic and non-enzymic reactions. Our results indicated that use of heat treatment prior to blend processing is an effective and feasible method to inhibit garlic discoloration and retain high content of bioactive OSCs. It is recommended that garlic paste be prepared using heated-blended processing, with thermal processing limited to 75 °C for less than 5 min.
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FOOD AND CHEMICAL TOXICOLOGY
·
2023
·
IF 4.3
Shuaishuai Chen,Weiyu Guo,Huan Liu,Jiang Zheng,Dingyan Lu,Jia Sun,Chun Li,Chunhua Liu,Yonglin Wang,Yong Huang,Wen Liu,Yongjun Li,Ting Liu
Psoralen and isopsoralen are the major components responsible for Psoraleae Fructus-induced hepatotoxicity. This study explored the role of metabolic activation by cytochrome P450 (CYP) enzymes in psoralen- and isopsoralen-induced cytotoxicity and its potential mechanisms. Inhibitors of CYP1A2 , 2C9, 2C19, 2D6, 2E1, and 3A4 were used to screen specific CYP enzymes responsible for the metabolic activation of psoralen and isopsoralen in mouse primary hepatocytes, which was verified using the corresponding transfected cell lines. Network toxicology and transcriptome analyses were performed to explore the mechanisms underlying toxicity. Psoralen and isopsoralen decreased the viability of mouse primary hepatocytes, whereas the inhibition of CYP2C9 , 2C19, 2D6, and 2E1 significantly increased their viability. Psoralen-induced cytotoxicity was significantly enhanced by the overexpression of CYP2C19 in Chinese hamster ovary cells, whereas the overexpression of the above CYP enzymes did not affect the cytotoxicity of isopsoralen. Psoralen- and isopsoralen-induced cytotoxic effects were associated with putative core targets (i.e., Fn1 , Thbs1 , and Tlr2) and multiple signaling pathways (e.g., PI3K-Akt, MAPK , and TNF pathways). Our results demonstrate that the metabolic activation of psoralen and isopsoralen is mediated by CYP enzymes, thereby regulating multiple core targets and signaling pathways and resulting in cytotoxicity.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 3
·1-9
·
IF 5.479
Wu Tiaodi,Li Lin,Song Guangjie,Ran Miaomiao,Lu Xiaoquan,Liu Xiuhui
A sensor is described for determination of superoxide anion (O 2 ˙ − ). The electrode consists of nitrogen-doped cotton carbon fiber (NCFs) modified with silver nanoparticles (AgNPs) which have excellent catalytic capability. The resulting sensor, best operated at working potentials around −0.5 V (vs. SCE), can detect O 2 ˙ − over an extraordinarily wide range that covers 10 orders of magnitude, and the detection limit is 2.32 ± 0.07 fM. The electrode enables the release of O 2 ˙ − from living cells under normal or under oxidative stress conditions to be determined. The ability to scavenge the superoxide anions of antioxidants was also investigated. In the authors’ perception, the method represents a viable tool for studying diseases related to oxidative stress.
Schematic presentation of the construction of an electrochemical sensor based on Nitrogen-doped cotton carbon fiber and silver nanoparticles. It can be used for the direct detection of superoxide anions released from Glioma cells (U87) under normal or under oxidative stress conditions.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Yanbin Su,Xiaowei Ma,Ning Jiang,Qingsong Zhang,Mengjie Li,Yuan Li,Shuxin Li
The purposes of this study were to probe spectral behaviors of the toxic targets in trans-crotonaldehyde (TCA) in mitochondria altered by diallyl disulfides (DADS) derived from garlic. Ultraviolet absorption spectra showed that when ethanol as a solvent, the DADS blue shifted the peak of TCA from 318 nm to 312 nm. In mitochondria, the DADS further blue displaced the peak of TCA from 312 nm to 308 nm. Raman spectra displayed that the S S of DADS directly interacted with the C C toxic target of TCA, then the C C C of DADS interacted with the CH O toxic target of TCA. When DADS to TCA was 1:2, the DADS was the most powerful for the removal of the C C and CH O toxic targets of TCA. Study suggested that the S S of DADS altered the C C toxic target of TCA, while the C C C of DADS eliminated the CH O toxic target of TCA via local electron delocalization . The above two together clearly depicted the spectral behaviors of the toxic targets of TCA in mitochondria altered by DADS. These results are of great significance and value to elucidate the effects of garlic organic polysulfide on myocardial ischemia for the extensive development and use of garlic extracts.
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JOURNAL OF MEDICAL ENTOMOLOGY
·
2015
·Vol. 52
·期 3
·437-441
·
IF 1.953
Liu Xin Chao,Zhou Ligang,Liu Qiyong,Liu Zhi Long
The aim of this research was to evaluate larvicidal activity of the essential oil of Allium tuberosum Rottler ex Sprengle roots and its constituents against larval mosquitoes (Aedes albopictus Skuse). Essential oil of A. tuberosum was obtained by hydrodistillation and analyzed by gas chromatography and gas chromatography–mass spectrometry. The major constituents of the oil were found to be sulfur-containing compounds, including allyl methyl trisulfide (35.19%), diallyl disulfide (28.31%), diallyl trisulfide (20.91%), and dimethyl trisulfide (12.33%). The essential oil of A. tuberosum exhibited larvicidal activity against the fourth-instar larvae of Ae. albopictus, with an LC50 value of 18 μg/ml. The constituents compounds—diallyl trisulfide (LC50 = 4 μg/ml) and diallyl disulfide (LC50 = 6 μg/ml)—possessed stronger larvicidal activity than allyl methyl trisulfide (LC50 = 27 μg/ml) and dimethyl trisulfide (LC50 = 35 μg/ml) against the fourth-instar larvae of Ae. albopictus. The results indicated that the essential oil of A. tuberosum and its major constituents have good potential as a source for natural larvicides.
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PHYTOTHERAPY RESEARCH
·
2024
·
IF 6.1
Jia-yi Dou,Zhen-yu Cui,Mei-yan Xuan,Chong Gao,Zhao-Xu Li,Li-hua Lian,Hao-zhen Cui,Ji-xing Nan,Yan-ling Wu
Environmental pollution, virus infection, allergens, and other factors may cause respiratory disease, which could be improved by dietary therapy. Allium species are common daily food seasoning and have high nutritional and medical value. Diallyl disulfide (DADS) is the major volatile oil compound of Allium species. The present study aims to explore the preventive effect and potential mechanism of DADS on pulmonary fibrosis. C57BL/6J mice were intratracheally injected with bleomycin (BLM) to establish pulmonary fibrosis and then administrated with DADS. Primary lung fibroblasts or A549 were stimulated with BLM, followed by DADS, farnesoid X receptor (FXR) agonist (GW4064), yes-associated protein 1 (YAP1) inhibitor (verteporfin), or silencing of FXR and YAP1. In BLM-stimulated mice, DADS significantly ameliorated histopathological changes and interleukin-1β levels in bronchoalveolar lavage fluid. DADS decreased fibrosis markers, HIF-1α, inflammatory cytokines, and epithelial-mesenchymal transition in pulmonary mice and activated fibroblasts. DADS significantly enhanced FXR expression and inhibited YAP1 activation, which functions as GW4064 and verteporfin. A deficiency of FXR or YAP1 could result in the increase of these two protein expressions, respectively. DADS ameliorated extracellular matrix deposition, hypoxia, epithelial-mesenchymal transition, and inflammation in FXR or YAP1 knockdown A549. Taken together, targeting the crosstalk of FXR and YAP1 might be the potential mechanism for DADS against pulmonary fibrosis. DADS can serve as a potential candidate or dietary nutraceutical supplement for the treatment of pulmonary fibrosis.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Jinlong Qin,Min Sun,Jiajing Cheng,Huici Jiang,Mingchen Lv,Jianxing Jing,Ran Chen,Zhen Fan,Jianzhong Du
Unavoidable damage to the basal layer of the endometrium has a huge negative impact on a woman’s reproductive health and menstrual cycle. However, it is difficult for medicine to penetrate a series of biological barriers toward the basal layer in the deeper area of the endometrium. To meet this challenge, we developed an ultrasound-responsive hydrogel incorporated with a transforming growth factor-beta (TGF-β) mimetic peptide to enhance pregnancy outcomes by restoring the function of a wounded endometrium due to its deep-tissue-penetration capability. In vitro studies revealed that the TGF-β-mimetic-peptide-loaded hydrogel could achieve 64.35% of cell migration under ultrasound stimulation even in phosphate-buffered saline of pH 6.0. Upon in situ sonication at the uterus, carboxymethyl chitosan can be released from degraded hydrogel to open tight junctions with reduced interstitial pressure by ultrasound to promote deep penetration. Rat studies revealed that the penetration capability of TGF-β-mimetic-peptide-loaded hydrogel with sonication was 1.6 times higher than that of the control group. Besides the rat uterine model, ex vivo human uterine tissue was also collected and imaged, demonstrating up to ∼700 μm of tissue penetration depth. In addition, compared to control groups, effective uterus recovery without intrauterine stenosis and endometrial cavity fluid was observed from rats with severe uterine injury treated by TGF-β-mimetic-peptide-loaded hydrogel. In addition, fertility restoration in the endometrial injury model was observed after treatment with such an ultrasound-responsive hydrogel incorporated with TGF-β mimetic peptides. Overall, this work provides an effective approach to treating endometrial injury for enhanced pregnancy outcomes.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Liangman Shi,Lunhui Zhu,Jialing Cao,Zhongyuan Zhang,Zhuqing Dai,Dajing Li,Lianfu Zhang,Cheng Yang
A novel mayonnaise product rich in cis ( Z )-lutein was developed by natural garlic oil and heat treatment. The formula of the mayonnaise was as follows: 68 % soybean oil, 7 % garlic oil, 14 % egg yolk, 6 % white vinegar, 3 % white sugar, and 2 % edible salt. After heated at 50 °C for 1 h, the ratio of Z- lutein in the mayonnaise significantly increased from 11.59 % to 40.47 %. The final products showed higher textural stability and relatively good sensory acceptance comparing with three commercial mayonnaises. The partition factor value of the ordinary mayonnaise was 46.50 %, while that of mayonnaise rich in Z -lutein reached 60.27 %. The bioaccessiblity of lutein in mayonnaise rich in Z -lutein was 31.72 % by simulated in vitro digestion and was twice of that in ordinary mayonnaise (14.68 %). We conclude, for the first time, garlic oil and thermal treatment improve the production of Z -lutein in the real food emulsion system.
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危险品化学品经营许可证（带存储）