INDUSTRIAL CROPS AND PRODUCTS
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2025
·
IF 6.2
Fulong Zhang,Xu Zeng,Wu Lan,Xuejin Zhang,Chuanfu Liu
The enzymatic hydrolysis of pretreated lignocellulose to produce value-added oligosaccharides presents the advantages of a simplified procedural approach with reduced environmental impact. The subsequent purification is necessary to improve the purity of oligosaccharides for further application. In this work, a high-yield xylooligosaccharides-rich hydrolysate was obtained by ball-mill-assisted alkaline peroxide (BAP) pretreatment and enzymatic hydrolysis, and the sequential separation of xylobiose and cellobiose was performed by activated carbon adsorption. Adsorption kinetics and isotherm models of saccharides on activated carbon were systematically investigated to understand the adsorption behavior. Results indicate that separation achieves a purity exceeding 70 %, with 38.21 % of xylobiose and 84.22 % of cellobiose being adsorbed. Density functional theory (DFT) calculations reveal the involvement of oxygen-containing groups on the activated carbon surface in sugar adsorption from the molecular level. The findings of this study establish a theoretical foundation for separating xylobiose and cellobiose from oligosaccharides hydrolysate through activated carbon adsorption.
研究领域:
生物化学
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可再生资源工程
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高影响因子
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
·
2022
·Vol. 49
·期 3
·
IF 4.258
Wang Lei,Zheng Peng,Hu Meirong,Tao Yong
Cellobiose, a natural disaccharide, attracts extensive attention as a potential functional food/feed additive. In this study, we present an inorganic phosphate (Pi) self-sufficient biotransformation system to produce cellobiose by co-expressing sucrose phosphorylase (SP) and cellobiose phosphorylase (CBP). The Bifidobacterium adolescentis SP (BASP) and Cellvibrio gilvus CBP (CGCBP) were co-expressed in Escherichia coli. Escherichia coli cells containing BASP and CGCBP were used as whole-cell catalysts to convert sucrose and glucose to cellobiose. The effects of reaction pH, temperature, Pi concentration, and substrate concentration were investigated. In the optimum biotransformation conditions, 800 mM cellobiose was produced from 1.0 M sucrose, 1.0 M glucose, and 50 mM Pi, within 12 hr. The by-product fructose and residual substrate (sucrose and glucose) were efficiently removed by treatment with yeast, to help purify the product cellobiose. The wider applicability of this Pi self-sufficiency strategy was demonstrated in the production of laminaribiose by co-expressing SP and laminaribiose phosphorylase. This study suggests that the Pi self-sufficiency strategy through co-expressing two phosphorylases has the advantage of great flexibility for enhanced production of cellobiose (or laminaribiose).
研究领域:
生物技术
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生物化学
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微生物学
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DOI
PubMed
高影响因子
COMBUSTION AND FLAME
·
2019
·
IF 4.12
Bin Hu,Qiang Lu,Zhen-Xi Zhang,Yu-Ting Wu,Kai Li,Chang-Qing Dong,Yong-Ping Yang
The fast pyrolysis mechanism of xylose , xylobiose and xylan was investigated with combined fast pyrolysis experiments and quantum chemistry calculations. The results indicate that acyclic d -xylose is an important intermediate for the formation of the major pyrolytic products in xylose pyrolysis, i.e., 1,4-anhydro- d -xylopyranose (ADX), furfural (FF) and hydroxyacetaldehyde (HAA). ADX derives from the successive cyclization of d -xylose which is the only source for ADX formation. 3-Deoxy-xylose is the most important intermediate for FF formation. HAA results from the C C bond scission by retro-aldol and cyclic Grob fragment. In addition, the five-membered intermediate derived from the ring-contraction reaction can transform into FF with high selectivity. The dehydrated xylose is also another source for HAA formation. The fast pyrolysis mechanism of xylobiose is similar to that of xylose, and thus similar pyrolytic product distribution can be obtained from them. However, the pyrolytic products of xylan differ significantly from those of xylose and xylobiose, which is ascribed to its high degree of polymerization and the branches in it.
研究领域:
化学工程
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生物质能
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2023
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IF 6.1
Simin Liu,Jiamei Liu,Linguo Zhao,Jianjun Pei
Glycosylation, one of the most common and significant modifications in nature, has prompted the development of a cellobiose phosphorolysis route for glycosylation in vivo. However, the process of glycosylation is hampered by the notably low conversion rate of cellobiose. In this work, regulation of the carbon source supply by changing the ratio of glucose to cellobiose improved the conversion rate of cellobiose, resulting in enhancing the efficiency of glycosylation and the production of vitexin. Moreover, three genes (pgm, agp, and ushA) involved in the degradation of UDP-glucose were knocked out to relieve the degradation and diversion of the cellobiose phosphorolysis route. Finally, through the optimization of conversion conditions, we observed a continuous enhancement in cellobiose conversion rate and vitexin production in BL21ΔushAΔagp-TcCGT-CepA, corresponding to an increased concentration of added glucose. The maximum production of vitexin reached 2228 mg/L with the addition of 2 g/L cellobiose and 6 g/L glucose, which was 312% of that in BL21-TcCGT-CepA with the addition of 2 g/L cellobiose. The conversion rate of cellobiose in BL21ΔushAΔagp-TcCGT-CepA reached 88%, which was the highest conversion rate of cellobiose to date. Therefore, this study presents a cost-effective and efficient method to enhance the conversion rate of cellobiose during the glycosylation process.
研究领域:
生物化学
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代谢工程
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PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
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2024
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IF 7.1
Xiaoyu Shi,Yang Yang,Xin Ye,Heng Zhong,Chunling Wang,Fangming Jin
Catalytic conversion of cellulosic biomass into polyols is an effective approach for biomass upgrading. However, the conversion involves cascade reactions which require various catalytic sites cooperation for activating specific steps, resulting in low efficiency. Here, we propose a bifunctional catalyst containing acid sites and hydrogenation capacity for polyol production with cellobiose as the model compound of cellulose. Phosphonic acids (PAs) containing either methyl (MPA) or aminomethyl (NH2MPA) tails were used to tune the Pd/Al2O3 catalyst to form monolayers, thereby modulating the surface physiochemical properties and introducing multiple catalytic sites. An increasing ratio of PdO/Pd and a strong metal–support interaction were observed after PA modification, which could boost the hydrogenation activity for cellobiose. Furthermore, Pd/Al2O3-MPA exhibited excellent hydrolytic hydrogenation activity due to the increase of Lewis acid sites with good durability under hydrothermal conditions by MPA modification, achieving a 78.5% yield of sorbitol at 180 °C for 5 h. A plausible mechanism for enhancing the synergistic effect of Pd and support over Pd/Al2O3-MPA was also proposed. This investigation provides a novel strategy for rationally designing hydrothermally stable bifunctional catalysts to utilize biomass in a more effective way.
研究领域:
催化化学
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生物质能
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DOI
高影响因子
Advanced Science
·
2024
·
IF 14.3
Yuanze Yu,Qian Zhang,Pengfei Zhang,Xu Jia,Hongjiang Song,Shengkui Zhong,Jie Liu
The practical application of aqueous Zn-ion batteries (AZIBs) is hindered by the crazy Zn dendrites growth and the H 2 O-induced side reactions, which rapidly consume the Zn anode and H 2 O molecules, especially under the lean electrolyte and Zn anode. Herein, a natural disaccharide, d -trehalose (DT), is exploited as a novel multifunctional co-solvent to address the above issues. Molecular dynamics simulations and spectral characterizations demonstrate that DT with abundant polar −OH groups can form strong interactions with Zn 2+ ions and H 2 O molecules, and thus massively reconstruct the coordination structure of Zn 2+ ions and the hydrogen bonding network of the electrolyte. Especially, the strong H-bonds between DT and H 2 O molecules can not only effectively suppress the H 2 O activity but also prevent the rearrangement of H 2 O molecules at low temperature. Consequently, the AZIBs using DT30 electrolyte can show high cycling stability even under lean electrolyte (E/C ratio = 2.95 µL mAh −1 ), low N/P ratio (3.4), and low temperature (−12 °C). As a proof-of-concept, a Zn||LiFePO 4 pack with LiFePO 4 loading as high as 506.49 mg can be achieved. Therefore, DT as an eco-friendly multifunctional co-solvent provides a sustainable and effective strategy for the practical application of AZIBs.
研究领域:
材料科学
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电化学
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能源存储
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DOI
PubMed
高影响因子
BIOMACROMOLECULES
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2021
·
IF 6.988
Ronghua Yin,Lutan Zhou,Na Gao,Lisha Lin,Huifang Sun,Dingyuan Chen,Ying Cai,Zhili Zuo,Kaifeng Hu,Shengxiong Huang,Jikai Liu,Jinhua Zhao
Glycosaminoglycans (GAGs) are conserved polysaccharides composed of linear repeating disaccharides and play crucial roles in multiple biological processes in animal kingdom. However, saccharide-branched GAGs are rarely found, except the fucose-branched one from sea cucumbers. There was conjecture about the presence of disaccharide-branched GAG since 30 years ago, though not yet confirmed. Here, we report a GAG containing galactose-fucose branches from Thelenota ananas. This unique branch was confirmed as d-Gal4S(6S)-α1,2-l-Fuc3S by structural elucidation of oligosaccharides prepared from T. ananas GAG. Bioassays indicated that oligomers with a larger degree of polymerization exhibited a potent anticoagulation by targeting the intrinsic tenase. Heptasaccharide was proven as the minimum fragment retaining the anticoagulant potential and showed 92.6% inhibition of venous thrombosis in vivo at sc. of 8 mg/kg with no obvious bleeding risks. These results not only solve a long-standing question about the presence of disaccharide-branched GAG in Holothuroidea, but open up new opportunities to develop safer anticoagulants.
研究领域:
生物化学
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海洋生物学
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药理学
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DOI
PubMed
高影响因子
Fermentation-Basel
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2025
·Vol. 11
·期 3
·119
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IF 3.3
Liang Wu,Yong Xu
Bacillus subtilis(B. subtilis) has booming application prospects in the feed industry as a value-added probiotic. Considering the intermediate and end saccharides of glucan and xylan digestion during lignocellulose biorefinery processing, the various digested saccharides were detected to disclose the bio-uptake and fermenting characteristics by solo or co-fermentation, especially with respect to five monosaccharides, cellobiose, and functional food additive—xylo-oligosaccharides (XOS).B. subtiliscan directly ferment XOS without xylose, achieving a cell yield of 0.71 g/g, while it hardly utilizes cellobiose except glucose co-fermentation. With a 5 g/L glucose addition and co-fermentation, bacterial cell yield was improved to 0.70 g/g. In total,B. subtilishad a 0.56–0.90 g/g cell yield from corncob-digested saccharides and the highest saccharide-uptaking rate of 98%. This study provides critical insights and experimental data to advance lignocellulosic biorefinery technologies, particularly for probiotics production and simplification of enzymatic hydrolysis, as well as the potential application in high value-added XOS biopurification.
研究领域:
生物工程
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微生物学
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DOI
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-7
·
IF 5.228
Qu Yongshui,Li Li,Wei Quanyuan,Huang Chongpin,Oleskowicz-Popiel Piotr,Xu Jian
Conversion of carbohydrate into 5-hydroxymethylfurfural (5- HMF), a versatile, key renewable platform compound is regarded as an important transformation in biomass-derived carbohydrate chemistry. A variety of ILs, not only acidic but also alkaline ILs, were synthesized and used as catalyst in the production of 5-HMF from disaccharide. Several factors including reaction temperature, IL dosage, solvent and reaction time,were found to influence the yield of 5-HMF from cellobiose. Of the ILs tested, hydroxy-functionalized ionic liquid (IL), 1-hydroxyethyl-3-methylimidazolium tetrafluoroborate ([AEMIM]BF 4 ) showed the highest catalytic activity and selectivity. 5-HMF yield of 68.71% from sucrose was obtained after 6 hrs at 160 °C. At the same condition with cellobiose as substrate, 5-HMF yield was 24.73%. In addition, 5-HMF also exhibited good stablity in this reaction system. Moreover, a kinetic analysis was carried out in both acidic and alkaline IL-catalyzed system, suggesting main side reaction in the conversion of fructose catalyzed by acidic and alkaline IL was polymerization of fructose and 5-HMF degradation, respectively.
研究领域:
生物质化学
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催化化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Baojun Feng,Hong Liao,Wenjun Ying,Junhua Zhang,Zhangjing Chen
Short-chain xylo-oligosaccharides (XOS) such as xylobiose (X2) and xylotriose (X3) have higher biological activities. Therefore, it is interesting to produce highly active XOS enriched with X2 and X3. In this work, pH-controlled lactic acid (LA) hydrolysis was used to produce XOS from xylan in moso bamboo and xylanase was used to convert high DP XOS into low DP XOS to increase the percentage of X2 + X3 in XOS. A 33.1 % XOS yield was obtained from 2 % LA hydrolysis (pH = 3.2). After xylanase hydrolysis of the LA hydrolysate, the total XOS yield reached 64.1 %, with X2 + X3 yield reaching 58.4 %. The percentage of X2 + X3 increased from 52.3 % to a high level of 91.0 %. The deep eutectic solvent pretreatment removed 87.2 % lignin from the residue and the glucose yield of the delignified residue hydrolyzed by cellulase was 96.9 %. The results suggested that the integrated process of LA and xylanase hydrolysis could effectively produce X2 + X3 from xylan in moso bamboo.
研究领域:
生物化学
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可再生资源与环境科学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Bin Li,Wenjing Gao,Ling Ling,Shaoning Yu,Liangqiang Chen,Fan Yang
Lactose is an important nutrient but its content in milk products poses concerns for people with lactose intolerance . Thus, a simple, rapid, and high-throughput assay for lactose determination is needed to maintain specified lactose levels during production and in final milk products. Here, we report a reactive matrix-assisted laser desorption/ionization mass spectrometry ( Re MALDI MS) method for the determination of lactose in milk products. The selective reactivity and the excellent laser absorption properties of 4-hydrazinoquinazoline (4-HQ) matrix increase the sensitivity of traditional MALDI MS analysis of lactose by 100 folds. After optimization of the method, lactose was detected distinctly in milk without pretreatment . Using xylobiose as an internal standard, the assay accurately quantified lactose in the concentration range of 1.00−700 μg/mL (R 2 = 0.994). Due to the high sensitivity and specificity, the lactose content in different types of milk samples was determined successfully, as well as the residual lactose in low-lactose and lactose-free milk.
研究领域:
食品科学
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分析化学
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DOI
高影响因子
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2021
·Vol. 36
·期 3
·e9218
·
IF 2.419
Mengying Du,Kailin Zhang,Luyang Jiao,Yicheng Xu,Xianglei Kong
Rationale The challenge of glycan identification due to their structural complexity and diversity has profited enormously from recent developments in mass spectrometry (MS)-related methods. For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis. Methods A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system was applied for the experiments. 3,5-Diiodo-L-tyrosine was selected as the assistant molecule to form complex ions with ten isomeric disaccharides through electrospray ionization. The complex ions were further isolated and irradiated by IR and UV lasers separately or continuously in the FTICR cell. Results By combining the two complementary fragment spectra generated from the IR and UV lasers, a clear identification of all the ten isomers was achieved using their binary codes based on their fragmentation patterns. The double-beam method simplifies the experiment by introducing the two lasers sequentially in one experiment, providing richer fragmentation patterns and making the full discrimination easier. Conclusions This study demonstrates the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers. The double-beam photodissociation method described here distinguished compositional, configurational and connectivity disaccharide isomers successfully. Compared with the data accumulation method based on separate IR and UV experiments, this method is simpler, faster, more flexible and also characterized by richer fragmentation patterns.
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）