MOLECULES
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2022
·Vol. 27
·期 15
·4876
·
IF 4.927
Yunping Wei,Jie Zhang,Xingcen Liu
Understanding the influence of surfactants on the assembly of peptides has a considerable practical motivation. In this paper, we systematically study the anionic surfactant-assisted assembly of diphenylalanine (FF). FF forms broom-like structures in a concentration of sodium cholate (NaC) around the CMC, and assembles into linear and unidirectional rods in the presence of low and high surfactant concentrations. FF’s improved hydrogen bonding and controlled assembly rates are appropriate for other anionic surfactants. At this stage, the use of FF as the simplest protein consequence can be helpful in the investigation of further protein–surfactant interactions.Keywords:dipeptide;broom-like structures;assembly;surfactant
研究领域:
生物化学
·
分子生物学
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2017
·
IF 2.896
Jun Wang,Qiuwen Li,Xiaojuan Li,Weiqi Yuan,Sichao Huang,Shaohui Cai,Jun Xu
Fibroblast activation protein-α (FAPα) is a serine protease of the post-prolyl peptidase family that is specifically expressed in the majority of human epithelial tumors , but not in normal tissues. In this study, we demonstrated the anti-tumor activity of a novel targeting drug formed by conjugating epirubicin (EPI) with an FAPα-specific dipeptide (Z-Gly-Pro) and named it Z-GP-EPI. Consistent with this tumor-targeting delivery strategy, the results illustrated that Z-GP-EPI could release EPI efficiently after incubating with FAPα and could exhibit similar antitumor effects as EPI in vitro in FAPα over-expressed tumor cells (4T1/FAPα + ). Furthermore, the evaluation of antitumor activity of Z-GP-EPI in vivo was implemented in a 4T1/FAPα + tumor-bearing mice xenograft model. Our results illustrated that Z-GP-EPI had similar antitumor effects in 4T1/FAPα + tumor-bearing mice and showed no visible cardiotoxicity side effects compared with free EPI. Thus, our study indicated that this FAPα-activated prodrug targeting strategy may provide a new mechanism for the targeted delivery of antitumor agents and improve their safety levels.
研究领域:
肿瘤学
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药理学
·
生物化学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Lin An,Shuhao Wang,Bingyu Liao,Jin Liu,Xiaoyan Jin,Yuyang Cai,Zibo Li,Yue Li,Junfeng Ma,Junbai Li
Self-assembled dipeptide hydrogel provides three-dimensional networks through noncovalent interactions for the embedment and the release of multiple biocomponents as well as for the growth of live cells. Here we demonstrate that Caenorhabditis elegans ( C. elegans ) as model organisms can be immobilized with significant vital signs in N -fluorenylmethoxycarbonyl diphenylalanine (Fmoc-FF) dipeptide hydrogel. With the controllability of dipeptide, assembly conditions for immobilization, growth and imaging of C. elegans were optimized. Compared with conventional anesthesia method, dipeptide hydrogel minimized unintended interferences for long-termed imaging. Particularly, nanofiber-based hydrogel can be diluted in solution as nanofiber dispersions alongside the release of C. elegans . By further analyzing the physiological phenotypes including basic growth, movement and reproduction once released from hydrogel, we confirmed the practicality and biocompatibility of Fmoc-FF hydrogel especially towards repeated immobilization and release of small-scaled living organisms, further broadening the scope of dipeptide hydrogel usage.
研究领域:
细胞生物学
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材料科学
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生物医学工程
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DOI
PubMed
高影响因子
iScience
·
2023
·Vol. 26
·期 7
·107048-107048
·
IF 5.8
Li Li,Zuxian Xu,Ruipin Cao,Jiaxin Li,Chang-Jer Wu,Yinglu Wang,Hu Zhu
We investigated the influence of hydroxyl groups on the anti-quorum-sensing (anti-QS) and anti-biofilm activity of structurally similar cyclic dipeptides, namely cyclo( L -Pro- L -Tyr), cyclo( L -Hyp- L -Tyr), and cyclo( L -Pro- L -Phe), against Pseudomonas aeruginosa PAO1. Cyclo( L -Pro- L -Phe), lacking hydroxyl groups, displayed higher virulence factor inhibition and cytotoxicity, but showed less inhibitory ability in biofilm formation. Cyclo( L -Pro- L -Tyr) and cyclo( L -Hyp- L -Tyr) suppressed genes in both the las and rh l systems, whereas cyclo( L -Pro- L -Phe) mainly downregulated rhl I and pqs R expression. These cyclic dipeptides interacted with the QS-related protein LasR, with similar binding efficiency to the autoinducer 3OC12-HSL, except for cyclo( L -Pro- L -Phe) which had lower affinity. In addition, the introduction of hydroxyl groups significantly improved the self-assembly ability of these peptides. Both cyclo( L -Pro- L -Tyr) and cyclo( L -Hyp- L -Tyr) formed assembly particles at the highest tested concentration. The findings revealed the structure-function relationship of this kind of cyclic dipeptides and provided basis for our follow-up research in the design and modification of anti-QS compounds.
研究领域:
微生物学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
POLYMER
·
2022
·
IF 4.432
Ning Zhang,Zhicheng Pan,Chao Li,Juan Wang,Yao Jin,Shaofeng Song,Mingwang Pan,Jinfeng Yuan
Self-repairing materials with damage self-healing ability have attracted much attention because of their potentials to improve the reliability, durability, and life of materials. However, its poor self-repair performance in the ambient environment still faces the challenge for waterborne polyurethane (WPU) broad range of applications. In this work, to break through the limitation of chain stiffness and improve the self-healing ability, we choose glycylglycine methyl ester as a functional end-capping reagent to seal the WPU chain (WPUG) and provide triple intermolecular hydrogen bonds . It is found that the chain-end triple hydrogen bonds act a unique role in WPUG, which can serve as a "bridge" to connect soft segments, endowing the molecular chain with more flexibility and fluidity . As a result, the self-healing efficiency of WPUG in the surrounding environment can reach 91.5% within a short time. This work suggests a new point of view on the role and interaction mechanism of chain-end hydrogen bonding in WPUs.
研究领域:
材料科学
·
化学工程
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DOI
高影响因子
Chemosensors
·
2022
·Vol. 10
·期 9
·360
·
IF 4.229
Xin Luo,Boya Ding,Xingcen Liu
Flexible and stretchable strain sensors can be applied for human health monitoring and disease diagnoses via the output of multiple biophysical signals. However, it is still a challenge to fabricate short-peptide-based strain sensors. Here, we prepared a novel polymer-dipeptide double-network hydrogel with excellent stretchability, responsiveness, and stability. The poly(acrylic acid) (PAA) gel, by cross-linking, maintains mechanical and flexible properties, and the fluorenyl methoxycarbonyl-diphenylalanine (Fmoc-FF) network, by non-covalent interactions, is helpful for energy dissipation. With increasing tensile or compression strains, the PAA/Fmoc-FF hydrogel exhibited a high mechanical strength and fast recovery. Moreover, as the presence of KCl improves the electronic conductivity, the hybrid gel exhibited a cyclic strain-stress performance, which is the foundation of a strain sensor. Based on that, its application as a motion sensor was demonstrated by monitoring the movements of human joints, such as the forefinger, wrist, elbow, and knee. Consequently, the hybrid polymer-peptide gel could be an ideal candidate for wearable sensors in the future.Keywords:strain sensor;double-network hydrogel;dipeptide;poly(acrylic acid)
研究领域:
材料科学
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生物医学工程
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DOI
高影响因子
Advanced Fiber Materials
·
2022
·Vol. 4
·期 3
·448-456
·
IF 12.958
Li Na,Ma Ying,Chen Niuniu,Tang Xiaoyu,Lv Shanshan
Silkworm silk and spider silk have been attracting numerous interests. Rapid solvation of silkworm silk protein and spider silk protein without hydrolysis of peptide bonds is highly desirable. Microwave irradiation has been proposed for facile extraction of water-soluble silk protein by various liquid media. However, microwave exposure can cause hydrolysis of peptide bonds, leading to irreversible degradation of silk protein. In this study, a series of representative dipeptides and a rationally designed recombinant protein derived from silk protein is employed to investigate the effect of microwave on the stability of the peptide bonds during a long time dissolution process, i.e., heating at 60 ℃ in a CaCl 2 :CH 3 CH 2 OH:H 2 O (1:2:8) solution. Results demonstrate that microwave irradiation imposes a minor damage and a negligible cleavage of the peptide bonds, compared with the conventional heating method. The microwave irradiation treatment suggested in this is suitable for dissolution of silk protein. It is anticipated that this approach can be developed to a commercial level commercially. Graphical abstract
研究领域:
生物化学
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材料科学
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DOI
高影响因子
Polymers
·
2020
·Vol. 12
·期 9
·2164
·
IF 3.426
Xinqiang Xu,Fuyan He,Wenke Yang,Jinshui Yao
As natural polymer materials, proteins are readily biodegradable, interestingly, the synthetic polyamides (PAs) that are based on the same amide bonds (also called peptide bonds in proteins) are barely degradable. Whether did the chirality and configuration of the amino acids play an important role. By using different configuration of amino acids, 4 types of polyamide-imides (PAIs) containing dipeptides ofLL,DL,LD, and DD configurations, respectively, were synthesized. It was found that the PAIs based on naturalLLconfiguration of dipeptide structure are much more readily biodegradable than those based on non-naturalLD,DL,andDDconfiguration of dipeptides. It was confirmed that the naturalL-configuration of amino acids play a critical role in degradability of proteins. And it also suggested that different type and amount of peptide fragments can be introduced in polymer to create series of polymer materials that can be biodegraded at controllable speed.Keywords:chiral amino acids;dipeptide;polyamide-imides;biodegradability
研究领域:
生物化学
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材料科学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Fuxing Xu,Shutong Yang,Fangling Wu,Chuan-Fan Ding
The functions and applications of dipeptides have been reported to possess unique characteristics compared to that of proteins or amino acids. Naturally occurring amino acids, except for glycine, are chiral molecules; thus, for each type of dipeptide there are four possible isomers. Isomeric structural analysis of dipeptides is important for measuring the quality of dipeptide products, as well as understanding their chemical and biological functions in humans. In the present study, a rapid and simple method for isomeric recognition of protonated dipeptides H-D/ l -Phe-D/L-X-OH, where X = hydrophobic amino acids, including alanine (Ala), isoleucine (Ile), leucine (Leu), proline (Pro), tryptophan (Trp), valine (Val), phenylalanine (Phe), and methionine (Met), was developed and investigated. Mixed solutions of dipeptides and cyclodextrins (CDs) were prepared for the production of gas phase, non-covalent complex ions by electrospray ionization (ESI). Trapped ion-mobility mass spectrometry (TIMS-MS) was subsequently used to investigate the corresponding ion mobilities (IM). The TIMS and density functional theory (DFT) results showed that the chiral structure of different dipeptides can be distinguished by β-CD, which indicates that different isomeric complexes have different collision cross sections (CCSs) and spatial conformations. The relative content of isomeric dipeptides mixtures was also obtained in the ratio range from 10:1 to 1:10 to measure the detection limits. Our work provides a direct analytical method for determining isomeric dipeptides, which could potentially have wide applications in the field of chiral recognition.
研究领域:
生物化学
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分析化学
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药物化学
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DOI
高影响因子
CHEMISTRY OF MATERIALS
·
2017
·
IF 9.466
Qiuju Li,Anan Sun,Yinsong Si,Min Chen,Limin Wu
Glutathione (GSH) plays a crucial role in various physiological processes. Therefore, efficient detection of GSH is of great importance. In this study, we present a new and highly efficient ratiometric fluorescent biosensor through one-pot synthesis of coupling the self-assembly of polysaccharide (alginate dialdehyde, ADA)-dipeptide (diphenylalanine, FF) and in situ reduction of gold nanoparticles (Au NPs) simultaneously encapsulating a dye (5-aminofluorescein, FI-NH2) inside to form ADA-Au-FF-dye nanospheres. Because the fluorescence of the dye can be quenched by Au NPs and linearly recovered following the introduction of different GSH concentrations owing to nanometal surface energy transfer on and off, this new ADA-Au-FF-dye hybrid nanosphere-based sensor allows label-free and ratiometric detection of intracellular GSH and other biothiols in a wide linear range, which is of great significance for cancer diagnostics and therapy.
研究领域:
生物化学
·
纳米医学
·
癌症诊断
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产品关联
DOI
高影响因子
LANGMUIR
·
2018
·
IF 3.789
Yajie Wang,Pengyao Xing,Wei An,Mingfang Ma,Minmin Yang,Tianxiang Luan,Ruipeng Tang,Bo Wang,Aiyou Hao
Facile control over preparation of organic building blocks and self-assembled aggregations to construct the desired materials remains challenges. This article reports selective dynamic covalent bonds formation and the corresponding self-assembly behaviors by using a dipeptide, glycylglycine (GlyGly), reacting with isomeric aromatic dialdehydes o-phthalaldehyde (OPA), p-phthalaldehyde (PPA), and m-phthalaldehyde (MPA) to demonstrate diversified aggregation forms caused by structure topology variations. Under alkaline condition, the aldehyde groups of phthalaldehydes can be connected with the amino groups of GlyGly by imine bonds as the dynamic chemical bonds. Owing to the fact that formation and dissociation of the imine bonds were reversibly pH-responsive, the reactions and aggregates assembled by their products were also reversibly controlled by changing pH. Three products, including two-armed product (OPGG, in which two GlyGly molecules were connected with one OPA molecule), single-armed product (PPG, in which only one GlyGly molecule was connected with a PPA molecule), and a mixture product (MPGG and MPG), as well as their different self-assembly behaviors, were obtained from OPA/GlyGly, PPA/GlyGly, and MPA/GlyGly systems, respectively, at the same condition of pH 8.6 in 90% methanol aqueous solution. However, for OPA/GlyGly system, another different type of product with benzopyrrole structure (OPG) was obtained by nucleophilic substitution via mixing OPA and GlyGly in water, which generated organic nanoparticles. Based on the results above, we conjectured the differences in dynamic covalent bond formation and supramolecular assembly clearly were influenced by the structure topologies of phthalaldehydes (OPA, PPA, and MPA). The experimental phenomenon verified the hypothesis as well, which may guide us to realize facile construction of selective reaction products and intelligent reversibly responsive materials with diverse morphologies and functions.
研究领域:
有机化学
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材料科学
·
生物化学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Yuqi Ma,Anhe Wang,Jieling Li,Qi Li,Qingquan Han,Yafeng Jing,Xuefang Zheng,Hongyu Cao,Xuehai Yan,Shuo Bai
The self-assembling behavior of peptides and derivatives is crucial in the natural process to construct various architectures and achieve specific functions. However, the surface or interfacial self-assembly, in particular, on the surface of micro- or nanoparticles is even less systematically investigated. Here, uniform porous CaCO3 microparticles were prepared with different charged, hydrophobic and hydrophilic surfaces to assess the self-assembling behavior of dipeptides composed of various sequences. Experimental results indicate that dipeptides with a negative charge in an aqueous solution preferred to self-assemble on the hydrophobic and positively charged surface of CaCO3 particles, which can be ascribed to the electrostatic and hydrophobic interaction between dipeptides and CaCO3 particles. Meanwhile, the Log p (lipid–water partition coefficient) of dipeptides has a significant effect on the self-assembling behavior of dipeptides on the surface of porous CaCO3; dipeptides with high Log p preferred to self-assemble on the surface of CaCO3 particles, resulting in the improved cell internalization efficiency of particles with low cytotoxicity. After loading with a model drug (doxorubicin), the particles show obvious antitumor activity in animal experiments and can reduce Dox side effects effectively.
研究领域:
生物材料学
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细胞生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）