MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Anqi Huang,Xiaofang Yang,Ting Xia,Daichun He,Rui Zhang,Zongtao Li,Shengchun Yang,Yong Liu,Xiaodong Wen
The excessive residue of copper ion (Cu 2+ ) has an important impact on the quality of traditional Chinese medicine (TCM). In this work, a sulfur quantum dots (SQDs) fluorescent probe was developed for rapid and sensitive detection of Cu 2+ in Paris polyphylla. Smith var. yunnanensis (Franch.) Hand. -Mazz. ( P. polyphylla var. yunnanensis ). SQDs were synthesized by the “assemble-fission” method. The synthesized SQDs were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM), and their fluorescence properties were studied. The prepared SQDs have advantages of ultra-small size, good optical properties and excellent stability. The addition of Cu 2+ could effectively quench the fluorescence of SQDs due to the inner filter effect (IFE) between Cu 2+ and SQDs. The detection linear range of this method was 20–200 μM. The limit of detection (LOD) was 6.78 μM. It has high selectivity in the simultaneous presence of other ions, and the possible fluorescence quenching mechanism was discussed in detail. Finally, the prepared SQDs were applied to the detection of actual samples of P. polyphylla var. yunnanensis with satisfactory results, which confirmed the great potential of SQDs in the actual measurement of Cu 2+ in TCM.
研究领域:
中药学
·
分析化学
·
生物医学
✓ 已验证
DOI
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Jue Liu,Yue-Xuan Liu,Yu-Song Song,Chen-Kai Liu,Fei-Xue Yu,Gao Liu,Tian-Rui Xu,Jun Sang
Ethnopharmacological relevance Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz., known as “Chonglou” in traditional Chinese medicine, is clinically used in cancer therapy, with saponins identified as the key antitumor constituents. However, the therapeutic potential and mechanisms of the total saponins from P. polyphylla var. yunnanensis (PPT) against bladder cancer, particularly their ability to overcome chemoresistance, remain unexplored. Aim of the study This study aimed to investigate PPT's anti-bladder cancer effects and mechanisms, with a focus on its potential to overcome cisplatin resistance. Materials and methods We established a UPLC-DAD method for saponin profiling and developed an eco-friendly extraction protocol using hydroxypropyl- β -cyclodextrin-assisted extraction combined with macroporous resin purification to prepare PPT. Anti-cancer efficacy was assessed in bladder cancer cell lines, mouse/zebrafish xenograft models, and cisplatin-resistant models. Mechanistic investigations integrated network pharmacology, metabolomics, molecular docking, and experimental validations (siRNA silencing, western blotting, flow cytometry, molecular dynamics simulations, cellular thermal shift assay, and surface plasmon resonance). Results The hydroxypropyl- β -cyclodextrin-based extraction system enhanced PPT purity by 15-fold, with the purified fraction showing dose-dependent anti-bladder cancer activity in vitro and in vivo . Mechanistically, PPT exerted anti-proliferative, pro-apoptotic, anti-lipogenic, and chemosensitizing effects by inhibiting the growth factor receptor binding protein 2 (GRB2)-mediated Akt-SREBP1 signaling axis. Notably, polyphyllin VII, a key constituent of PPT, was identified as a novel GRB2 inhibitor, potently suppressing bladder cancer progression and overcoming cisplatin resistance. Conclusion This study demonstrates that targeting the GRB2-Akt-SREBP1 signaling axis is a promising strategy for bladder cancer therapy, further establishing PPT as a multicomponent phytomedicine and PPVII as a novel GRB2 inhibitor with high translational potential.
研究领域:
癌症研究
·
药理学
·
分子生物学
·
民族药理学
·
生物信息学
·
信号转导
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）