Journal of Oceanology and Limnology
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2023
·Vol. 41
·期 5
·1965-1977
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IF 1.6
Liu Yan,Kociolek John Patrick,Kulikovskiy Maxim,Glushchenko Anton,Yu Pan,Wang Quanxi,Lu Xinxin,Fan Yawen
An interesting group of cymbelloid diatoms was collected from lakes in Yunnan Province, China, and observations on their valve morphology made using light and scanning electron microscopy. These cymbelloid species all have apical pore fields, slit-like areolae, and areolar occlusions, and they all lack of stigmata. In this paper we propose the new genus Qinia gen. nov. for these taxa and describe them as new: Q. lashii sp. nov., Q. aequalis sp. nov., and Q. daliensis sp. nov. The genus Qinia gen. nov. has lanceolate valves asymmetrical about their apical axes, raphe located in the middle of valve, distal raphe ends bent to the dorsal margin that bisects the apical pore fields (APFs), no stigmata, areolae with slit-like external openings and projections occluding the areolar openings internally. This genus resembles the genus Cymbella in the presence of APFs, but differs from Cymbella by a lack of stigmata, and by having external distal raphe ends that bisect the apical pore fields. Like Reimeria , the external distal raphe ends bisect the lobes of the APF, but Qinia species differ from Reimeria in that they have no stigmata and the distal raphe ends are deflected dorsally, not ventrally. Comparisons were made between Qinia and other cymbelloid genera whose members possess apical pore fields. Features that distinguish each species of the new genus are also discussed.
研究领域:
植物分类学
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淡水生态学
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藻类生物学
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形态学
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系统发育研究
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DOI
高影响因子
CURRENT DRUG METABOLISM
·
2024
·Vol. 25
·期 5
·317-329
·
IF 2.1
Xiaocui Li,Qi Liang,Caiyan Wang,Huawei Qiu,Tingting Lin,Wentao Li,Rong Zhang,Zhongqiu Liu,Lijun Zhu
BackgroundYunaconitine (YAC) is a hidden toxin that greatly threatens the life safety of patients who are prescribed herbal medicines containing Aconitum species; however, its underlying mechanism remains unclear.ObjectiveThe objective of this study is to elucidate the functions of P-glycoprotein (P-gp) in regulating the efficacy, toxicity, and pharmacokinetics of YAC.MethodsThe efflux function of P-gp on YAC was explored by using Caco-2 monolayers in combination with the P-gp inhibitor verapamil. The impact of P-gp on regulating the analgesic and anti-inflammatory effects, acute toxicity, tissue distribution, and pharmacokinetics of YAC was determined via male Mdr1a gene knocked-out mice and wild-type FVB mice.ResultsThe presence of verapamil significantly decreased the efflux ratio of YAC from 20.41 to 1.07 in Caco-2 monolayers (P < 0.05). Moreover, oral administration of 0.07 and 0.14 mg/kg YAC resulted in a notable decrease in writhing times in Mdr1a-/- mice by 23.53% and 49.27%, respectively, compared to wild-type FVB mice (P < 0.05). Additionally, the deficiency of P-gp remarkably decreased the half-lethal dose (LD50) of YAC from 2.13 to 0.24 mg/kg (P < 0.05). Moreover, the concentrations of YAC in the tissues of Mdr1a-/- mice were statistically higher than those in wild-type FVB mice (P < 0.05). Particularly, the brain accumulation of YAC in Mdr1a-/- mice significantly increased by 12- and 19-fold, respectively, after oral administration for 30 and 120 min, when compared to wild-type FVB mice (P < 0.05). There were no significant differences in the pharmacokinetic characteristics of YAC between Mdr1a-/- and wild-type FVB mice.ConclusionYAC is a sensitive substrate of P-gp. The absence of P-gp enhances the analgesic effect and toxicity of YAC by upregulating its brain accumulation. Co-administration with a P-gp inhibitor may lead to severe YAC poisoning.
研究领域:
药理学
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毒理学
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中药学
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DOI
PubMed
高影响因子
Sustainability
·
2022
·Vol. 14
·期 5
·2614
·
IF 3.889
Jieming Zhu,Chen Chen,Lie You
Serious farmland scarcities make smallholders a default mode for China’s agriculture, which makes efficient and equitable rural development a great challenge. Tensions lead to alternation between autonomous family farming and coordinated collective agriculture. Rapid urbanization since the 1980s has strongly stimulated flower-growing and agribusiness in Yunnan, China. The organization for commercial flower-farming is, however, an issue. Officially promoted for collective farming, voluntary cooperatives are wrecked by the free-riding problem. Grower associations nevertheless spontaneously emerge, with the flexible entry and exit of members without binding joint-assets and joint-ownership, which is facilitated by technological changes to the transaction. Empirical investigation in Tonghai, Yunnan, unveiled the institution of agribusiness–smallholder partnership for inclusive rural development. Smallholders have actively participated in flower agriculture, which has contributed significantly to the development of rural economies. The high casualty of micro-smallholders suggests that farm size is an important and crucial factor for sustainable farming. Effective rural development has to be supported by endogenous non-agricultural jobs so that farm size can be increased.
研究领域:
农业经济学
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社会学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 3.1
Peng Yijin,Yun Yurou,Zou Wenhui,Zhou Linyan
Our study analysed the quality differences between 6 pomegranate varieties grown in Yunnan. The results showed that the composition and contents of anthocyanins, the amount of polymeric pigments produced by the reaction between anthocyanins and tannins, and pH were the primary factors affecting the colour of pomegranate arils. Fructose and citric acid were the main sugar and organic acid contributing to the taste of pomegranate arils. In total, 42 phenolic compounds were identified, with BS being classified as a distinct group due to higher concentrations of ACNs and flavonols. Additionally, 32 volatile compounds were identified, with TLZ, SP, and TGY grouped, BS, TNS, and HRY grouped into another. The key phenolic metabolic pathways included flavone and flavonol biosynthesis, anthocyanin biosynthesis, and phenylpropanoid biosynthesis, while fatty acid biosynthesis, pyruvate metabolism, and sulfur metabolism were the main volatile metabolic pathways. These results provide valuable guidance for agricultural practices and promoting industrial development.
研究领域:
食品科学
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植物化学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Anqi Huang,Xiaofang Yang,Ting Xia,Daichun He,Rui Zhang,Zongtao Li,Shengchun Yang,Yong Liu,Xiaodong Wen
The excessive residue of copper ion (Cu 2+ ) has an important impact on the quality of traditional Chinese medicine (TCM). In this work, a sulfur quantum dots (SQDs) fluorescent probe was developed for rapid and sensitive detection of Cu 2+ in Paris polyphylla. Smith var. yunnanensis (Franch.) Hand. -Mazz. ( P. polyphylla var. yunnanensis ). SQDs were synthesized by the “assemble-fission” method. The synthesized SQDs were characterized by Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM), and their fluorescence properties were studied. The prepared SQDs have advantages of ultra-small size, good optical properties and excellent stability. The addition of Cu 2+ could effectively quench the fluorescence of SQDs due to the inner filter effect (IFE) between Cu 2+ and SQDs. The detection linear range of this method was 20–200 μM. The limit of detection (LOD) was 6.78 μM. It has high selectivity in the simultaneous presence of other ions, and the possible fluorescence quenching mechanism was discussed in detail. Finally, the prepared SQDs were applied to the detection of actual samples of P. polyphylla var. yunnanensis with satisfactory results, which confirmed the great potential of SQDs in the actual measurement of Cu 2+ in TCM.
研究领域:
中药学
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分析化学
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生物医学
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DOI
高影响因子
FOOD CHEMISTRY
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2014
·
IF 3.259
Longsheng Chen,Wei Xu,Rong Shao,Xianfeng Du
The profile and bioactivity of phytochemicals in Araiostegia yunnanensis ( Christ ) Cop were investigated. The total flavonoids content in A. yunnanensis is about 84.90 mg/g. By means of HPLC–DAD–ESI-MS, the main flavonoids in A. yunnanensis were tentatively identified as myricetin 3- O -rhamnosylglucoside, eriodictyol 7- O -rutinoside, quercetin 3- O -rutinoside, luteolin-7- O -apiosylglucoside, quercetin 3- O -rhamnosylgalactoside, and luteolin 7- O -glucoside. The extract (0.268 mg/ml total flavonoids) from A. yunnanensis showed very strong superoxide anion radical scavenging potential and reducing power, which are higher than those of rutin (0.25 mg/ml). The extract (0.268 mg/ml total flavonoids) from A. yunnanensis exhibited similar DPPH scavenging activity with rutin (0.25 mg/ml). However, rutin (0.25 mg/ml) showed a significantly higher ABTS radical scavenging effect than that of the extract (0.268 mg/ml total flavonoids) from A. yunnanensis. The methanol extract from A. yunnanensis showed obviously cytotoxic effects on A549 cells and it had no effect against acetylcholinesterase.
研究领域:
植物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2025
·
IF 4.2
Zhang Xiaowei,Wang Shuqi,Zhang Ke,Feng Liang,Xu Anchuan,Zhong Risheng,Chen Dan,Wang Chunqiong,Zhang Jiwu,Wan Yueying,Long Jie,Chen Haitao
Cigar is a flavor-dependent cash crop. However, the key aroma compounds of tobacco leaves are less studied. In this study, we used molecular sensory science to explore the key aroma compounds of cigar tobacco leaves from three different origins in Yunnan, China. The results showed that a total of 33 aroma compounds were quantitatively analyzed in the three tobaccos, among which there were eight key aroma components in YXYY, eight in DHYY, and four in PEYY with odor activity value (OAV)≥1 and flavor dilution (FD)≥2. Through recombination and omission experiments, the key aroma actives were further identified as phytol, acetic acid, isovaleric acid, 3-methylpentanoic acid, and (E)-5-isopropyl-8-methylnona-6,8-dien-2-one in YXYY, styrene, (E)-5-isopropyl-8-methylnona-6,8-dien-2-one, irisone, and phytol in DHYY, and acetic acid, styrene, and phytol in PEYY. In conclusion the present study revealed the key aroma compounds and their differences in cigar tobacco from three different origins. It provides insights for a comprehensive exploration of the unique flavors of cigars.
研究领域:
农业科学
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食品科学
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植物化学
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产品关联
DOI
PubMed
高影响因子
Journal of Analytical Methods in Chemistry
·
2024
·Vol. 2024
·期 1
·9583022
·
IF 2.3
Yuping Wu,Haiyu Zhang,Wenyuan Wang,Guanghui Kong,Zaiming Li,Tikun Zhang,Miaochang Wang,Dong Yang,Chengming Zhang,Yongping Li,Jin Wang
To characterize volatile organic compounds (VOCs) and aromatic sensory properties in Yun cigar, 27 samples from four origins were analyzed using SPME–HS–GC/MS and sensory analysis. The investigation results were analyzed using principal component analysis (PCA), Fisher linear discriminant analysis (LDA), and Pearson correlation analysis. In Yunnan cigars, the content of nicotine and neophytadiene accounted for over 90% of the total VOC content. Nicotine was significantly positively correlated with neophytadiene and phytol. The cigars from four origins were clearly classified by the PCA of VOCs. Four region discrimination functions were established through the LDA of 14 compounds, and the validation accuracy was 100%. The sensory descriptors with the highest geometric mean were woody, roasted, fresh-sweet, bean, and scorched. Acetophenone, megastigmatrienone A, and thunbergene were positively correlated with multiple aroma descriptors, while nicotine was negatively correlated with multiple aroma descriptors.
研究领域:
植物化学
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食品科学
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分析化学
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DOI
PubMed
高影响因子
Polymers
·
2022
·Vol. 14
·期 19
·4127
·
IF 4.967
Xiaobao Li,Zhengjie Tang,Zhenbing Sun,John Simonsen,Zhinan Luo,Xiaoping Li,Jeffery J. Morrell
Carboxymethyl cellulose (CMC) is a cellulose derivative that can be obtained from wood, bamboo, rattan, straw, and other cellulosic materials. CMC can be used to produce biofilms for many purposes, but the properties of these resulting films make them unsuitable for some applications. The effects of three kinds of plant fiber addition on CMC film properties was investigated using CMC derived from eucalyptus bark cellulose. Tensile strength (TS) and elongation at break (EB) of CMC/sodium alginate/glycerol composite films were 26.2 MPa and 7.35%, respectively. Tensile strength of CMC composite films substantially increased, reaching an optimum at 0.50 g of fiber. The enhancement due to industrial hemp hurd fiber on CMC composite films was more obvious. Pretreatment with hydrogen peroxide (H2O2) and glacial acetic acid (CH3COOH) produced films with a TS of 35.9 MPa and an EB of 1.61%. TS values with pectinase pretreated fiber films was 41.3 MPa and EB was 1.76%. TS of films pretreated with pectinase and hemicellulase was 45.2 MPa and EB was 4.18%. Chemical and enzymatic treatment both improved fiber crystallinity, but film tensile strength was improved to a greater extent by enzymatic treatment. Surface roughness and pyrolysis residue of the film increased after fiber addition, but Fourier transform infrared spectroscopy (FTIR), opacity, and water vapor transmission coefficients were largely unchanged. Adding fiber improved tensile strength of CMC/sodium alginate/glycerol composite films and broadened the application range of CMC composite films without adversely affecting film performance.Keywords:eucalyptus bark;Yunnan pine wood;bamboo culms;industrial hemp hurd;FTIR;XRD;TG;mechanical properties
研究领域:
材料科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2025
·
IF 4.8
Xiaowei Zhang,Tianyu Dong,Dan Chen,Haowei Sun,Anchuan Xu,Chunqiong Wang,Dan Li,Jieyun Cai,Lingduo Bu,Ke Zhang,Haitao Chen
The flavor of cigar smoke directly influences the consumer’s willingness to purchase. However, the analysis of cigar smoke was lack. In this research, a total of 235 volatile compounds were identified by GC×GC-TOFMS in cigar smoke from Yuxi, Dehong and Pu’er regions of Yunnan, including 72 alkenes, 47 aromatic hydrocarbons, 42 heterocyclics, 28 ketones, 8 alcohols, 4 aldehydes, 3 esters, 13 phenols, 5 acetonitrile and 13 others. ROAV analysis indicated that o-cresol, guaiacol, 2,6-dimethyl-phenol, 4-hydroxy-3-methoxystyrene, (E,E)-2,4-hexadienal, butylbenzene, naphthalene, nicotine and D-limonene might be the key flavor compounds. By OPLS-DA, 2-methyl-pyridine, 2,4-dimethylfuran, 2-carene, 13-heptadecyn-1-ol, 1-ethyl-2-methyl-benzene, 2,3,5,8-tetramethyl-1,5,9-decatrien, 1-ethyl-4-methyl-benzene, β-elemene, methyl isobutyl ketone and 3-methylcyclopentanone were found to be the differential volatiles distinguishing samples from different areas. Eventually, 5 groups of potential flavor precursors were summarized and identified (cembranoid, phenylalanine, chlorophyll, carotenoid and reducing sugar). This research has provided theoretical guidance for the improvement of flavor quality of cigar smoke in Yunnan.
研究领域:
分析化学
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食品科学
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烟草化学
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代谢组学
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风味化学
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产品关联
DOI
PubMed
高影响因子
Plants-Basel
·
2022
·Vol. 11
·期 6
·823
·
IF 4.658
Sifan Mei,Yanyan Cao,Gang Zhang,Su Zhou,Yi Wang,Shuying Gong,Qiang Chu,Ping Chen
Baking is widely accepted for aroma enhancement of black tea, and studies have mainly focused on the aroma or chemical substances under a specified baking condition. Understanding of the feedback between aroma substances and characteristics is urgently needed. Therefore, a mutual feedback platform (SES/MS) combined sensory evaluation system (SES) with gas chromatography–mass spectrometry (GC-MS) was established. Based on this platform, we found that baking at 90 °C for 4 h or 5 h could maintain the primary aroma attributes and increase characteristic aroma attributes—these were considered to be the best baking conditions for Yunnan congou black tea. Meanwhile, 47 volatiles were identified, among which, pyrrole and benzaldehyde appeared to have a caramel aroma, and 2-furanmethanol and α-terpineol presented a baked aroma. This study reveals the dynamic change of aroma profiles and compounds during the aroma enhancement, and provides an optional template for researchers, focused on the relationship between quality and aroma attributes of teas.Keywords:Yunnan congou black tea;aroma enhancement;aroma attributes;volatile compounds;multiple factor analysis
研究领域:
食品科学
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分析化学
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农学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2025
·
IF 5.4
Jue Liu,Yue-Xuan Liu,Yu-Song Song,Chen-Kai Liu,Fei-Xue Yu,Gao Liu,Tian-Rui Xu,Jun Sang
Ethnopharmacological relevance Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz., known as “Chonglou” in traditional Chinese medicine, is clinically used in cancer therapy, with saponins identified as the key antitumor constituents. However, the therapeutic potential and mechanisms of the total saponins from P. polyphylla var. yunnanensis (PPT) against bladder cancer, particularly their ability to overcome chemoresistance, remain unexplored. Aim of the study This study aimed to investigate PPT's anti-bladder cancer effects and mechanisms, with a focus on its potential to overcome cisplatin resistance. Materials and methods We established a UPLC-DAD method for saponin profiling and developed an eco-friendly extraction protocol using hydroxypropyl- β -cyclodextrin-assisted extraction combined with macroporous resin purification to prepare PPT. Anti-cancer efficacy was assessed in bladder cancer cell lines, mouse/zebrafish xenograft models, and cisplatin-resistant models. Mechanistic investigations integrated network pharmacology, metabolomics, molecular docking, and experimental validations (siRNA silencing, western blotting, flow cytometry, molecular dynamics simulations, cellular thermal shift assay, and surface plasmon resonance). Results The hydroxypropyl- β -cyclodextrin-based extraction system enhanced PPT purity by 15-fold, with the purified fraction showing dose-dependent anti-bladder cancer activity in vitro and in vivo . Mechanistically, PPT exerted anti-proliferative, pro-apoptotic, anti-lipogenic, and chemosensitizing effects by inhibiting the growth factor receptor binding protein 2 (GRB2)-mediated Akt-SREBP1 signaling axis. Notably, polyphyllin VII, a key constituent of PPT, was identified as a novel GRB2 inhibitor, potently suppressing bladder cancer progression and overcoming cisplatin resistance. Conclusion This study demonstrates that targeting the GRB2-Akt-SREBP1 signaling axis is a promising strategy for bladder cancer therapy, further establishing PPT as a multicomponent phytomedicine and PPVII as a novel GRB2 inhibitor with high translational potential.
研究领域:
癌症研究
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药理学
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分子生物学
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民族药理学
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生物信息学
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信号转导
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）