CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Jiayun Xu,Chuanling Si,Lin Dai,Jarl Hemming,Andrey Pranovich,Chunlin Xu
Lignin-first fractionation (microwave-assisted deep eutectic solvent fractionation, MW-DES) and carbohydrate-first fractionation (hot water extraction followed by MW-DES, HWE-MW-DES), were compared while pretreating spruce heartwood (HW) and sapwood (SW) for lignin extraction. The lignin recovery yield was 80 % for HW and 74 % for SW, while SW demonstrated high selectivity in delignification, achieving 86 % removal of lignin with only minor cellulose loss (3.6 %) treated with ChCl/LA (1:2) at 145 °C for 10 min. The carbohydrate-first approach gives an opportunity to obtain lignin with a lower carbohydrate content (∼0.5 %) and higher lignin purity (88 %). The structure of lignin exhibited no significant differences after two different fractionation approaches, with the phenolic hydroxyl content in lignin in the amount of 2.59/2.73 mmol/g (MW-DES) and 2.54/2.74 mmol/g (HWE-MW-DES) from HW/SW. The above provides a theoretical basis for developing and optimizing the valorization of lignin from spruce HW/SW, employing either lignin-first or carbohydrate-first fractionation approaches of the sustainable biorefinery processes.
研究领域:
林产化学与工业
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生物质能工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yibo Li,Shulei Li,JieYing Yuan,Zheyun Liu,Xiaozhou Song,Li Zhang,Lili Jia,Xiangyu Li,Ruijin Yu,Jiaxing Zhang,Yunjing Lu,Shanshan Lv,Kangren Niu,Nyuk Ling Ma,Jie Chu
In spruce, hemicelluloses containing acetyl groups hinder cellulase accessibility to cellulose, which reduces hydrolysis efficiency. This study investigated the effect of acetylation on cellulose hydrolysis. With an addition of 2–8 mg/mL, natural spruce galactoglucomannan (GGM) exhibited inhibition rates of 17.66 %–26.64 % on cellulose hydrolysis. Deacetylated galactoglucomannan (DGM) further intensified hydrolysis inhibition, reaching up to 41.95 % at 8 mg/mL. Conversely, the addition of 2 mg/mL of highly acetylated galactoglucomannan (AGM) significantly alleviated hydrolysis inhibition, thereby reducing inhibition by 76.44 %. The inhibition effect of acetylation on cellulose hydrolysis was directly proportional to AGM amount and inversely proportional to hydrolysis time, as confirmed by cellulose adsorption experiments. Infrared spectroscopy and nuclear magnetic resonance spectroscopy revealed structural changes in GGM due to acetylation and deacetylation. The effect of mannan on cellulase activity was examined via fluorescence spectroscopy. Steric hindrance and electrostatic repulsion from acetyl groups on mannan reduced enzyme adsorption. Highly acetylated mannose enhanced cellulase stability. These findings provide new insights for biorefining applications in spruce.
研究领域:
生物化学
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可再生资源工程
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DOI
PubMed
高影响因子
GREEN CHEMISTRY
·
2025
·
IF 9.2
He Wan,Yang Liu,Chunhui Yu,Kuiyuan Cao,Yongwei Han,Zhong Sun,Junyou Shi,Xixin Duan
Mild condition and high selectivity are challenging in oxidative depolymerization of lignin to phenolic compounds. In this study, choline chloride (ChCl) was introduced to selectively prepare monophenol compounds from the fractionation of larch lignin with the catalyst of g-C3N4 (CN) supported by H4PMo11VO40 (HPMoV). When the yield of bio-oil was 35%, the novel product of 1-(4-hydroxy-3- methoxyphenyl) butan-1-one (HMB) was obtained with the 11% yield under optimized conditions of 0.2 g 25% HPMoV/CN, ChCl: p-toluene sulfonic acid (PTS) (molar ratio 1:2), 1 mL H2O, 6 h, 80 °C, 220 W xenon lamp and air atmosphere. Results from different deep eutectic solvents (DES) indicated that ChCl was essential for HMB generation. The cleavage of β-O-4 bonds was dominant in the fraction of larch lignin and five representative lignin model compounds (LMCs) under optimum conditions. In addition, distinct degradation mechanisms of native lignin and different LMCs were deduced separately. Compared with other reported products in depolymerizing lignin by chemical oxidative procedures, the presentation of HMB was different because of the conjugate addition of ·CH3 from ChCl accompanying the participation of H2O. Overall, the integrative catalysis from acidic DES, strong oxidizing HPMoV, and visible light in this work provides new insights for valorizing lignocellulose into value-added aromatic products in an ambient, convenient, and photocatalytic reaction system.
研究领域:
化学工程
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生物质转化
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光催化材料
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产品关联
DOI
高影响因子
Solar RRL
·
2022
·Vol. 6
·期 9
·2200483
·
IF 9.173
Ziyi Shen,Deqi Fan,Hao Zhang,Jiapeng Jing,Haolan Xu,Huihua Min,Yi Lu,Xiaofei Yang,Jingsan Xu
With the increase in water consumption and pollution resulting from the rising world population and industrial development, severe fresh water shortage has been regarded as one of the critical problems facing the world. Solar-driven water purification is an environment friendly and promising technology to address the problem. However, low photothermal conversion efficiency impedes its practical application. Herein, a natural spruce wood-based solar evaporator functionalized with zeolitic imidazolate framework (ZIF-8) nanoparticles and polydopamine (PDA) layers is designed, which significantly reduces the equivalent evaporation enthalpy and substantially boosts solar evaporation efficiency. The evaporation rate of the optimized wood-based evaporator reached 2.28 kg m −2 h −1 with a high evaporation efficiency of 87.5% under 1.0 sun. Furthermore, the integrated spruce wood/ZIF-8/PDA hybrids can remove organic pollutants after solar evaporation. Notably, the constructed multifunctional solar evaporator takes advantage of sustainable solar energy, low-cost biomass, and ZIF-8/PDA nanostructures to acquire desirable performance in water evaporation and sewage purification.
研究领域:
材料科学
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环境科学
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DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
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2021
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IF 5.645
Hao-Nan Zhang,Hao Ren,Hua-Min Zhai
The structure of kraft lignin is heterogeneous and the reaction activity is low due to the complicated and somewhat uncertain chemical reactions under the strong alkali and high temperature conditions of kraft pulping chemistry. At present, people still lack understanding of its structure, so it is difficult to be used as high quality industrial raw material for many years despite its huge output. In this paper, the phenolation potential of industrial Spruce Kraft Lignin (SKL) in phase separation system was explored. Compared with previous studies on phase separation reaction, due to the direct use of lignin as feedstock , sulfuric acid would only act as a phenolation catalyst, and is no longer used for swelling cell walls and hydrolyzing carbohydrates , so a modest reduction of acid concentration is feasible. In the phase separation system, the benzyl position of lignin was activated to selectively graft the p -cresol groups. Due to the protection of organic phase on lignin, the breakage of the β linkages and the condensation reaction of lignin were effectively prevented, so the original structure of lignin was well preserved. In order to capture the detailed structural changes of lignin after phenolation in phase separation system, FT-IR, GPC and quantitative NMR techniques ( 1 H-NMR, 31 P-NMR and 2D-NMR) were used to analyze the changes of main functional groups, linkages and substructures of industrial lignin samples before and after phenolation. The results showed that the industrial spruce kraft lignin still have many active sites which can be modified by phenolation. And through p -cresol treatment, a kind of high activity industrial lignin with more phenolic hydroxyl groups, more uniform molecular size and higher purity was obtained. Then the molecular structure models of spruce kraft lignin before and after phenolation is proposed respectively based on the molecular weights, quantitative analysis of functional groups, linkages, substructures and introduced p -cresols. This will be helpful to understand the structural changes of industrial spruce kraft lignin during phenolation in phase separation system, and provide guidance for the high value utilization of this high activity industrial lignin in the future.
研究领域:
林产化学
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生物质化学
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产品关联
DOI
高影响因子
JOURNAL OF EXPERIMENTAL BOTANY
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2020
·Vol. 72
·期 5
·1748-1763
·
IF 5.908
Wang Li,Liu Jing,Shen Yitong,Pu Ruolan,Hou Meiying,Wei Qiang,Zhang Xinzhen,Li Guishuang,Ren Hongyan,Wu Guang
Brassinosteroids (BRs) are essential plant hormones. In angiosperms, brassinolide and castasterone, the first and second most active BRs, respectively, are synthesised by CYP85A2 and CYP85A/A1, respectively. BRs in angiosperms function through an essential receptor, BR Insensitive 1 (BRI1). In addition, some angiosperms also have non-essential BRI1-like 1/3 (BRL1/3). In conifers, BRs promote seed germination under drought stress; however, how BRs function in gymnosperms is unknown. In this study, we performed functional complementation of BR biosynthesis and receptor genes from Picea abies with respective Arabidopsis mutants. We found that P. abies possessed functional PaCYP85A and PaBRL1 but not PaCYP85A2 or PaBRI1, and this results in weak BR signaling, and both PaCYP85A and PaBRL1 were abundantly expressed. However, neither BR treatment of P. abies seedlings nor expression of PaBRL1 in the Arabidopsis Atbri1 mutant promoted plant height, despite the fact that BR-responsive genes were activated. Importantly, chimeric AtBRI1 replaced with the BR-binding domain of PaBRL1 complemented the Atbri1 phenotypes. Furthermore, PaBRL1 had less kinase activity than BRI1 in vitro. Overall, P. abies had weak but still active BR signaling, explaining aspects of its slow growth and high stress tolerance. Our study sheds light on the functional and evolutionary significance of distinct BR signaling that is independent of BRI1 and brassinolide.
研究领域:
植物生物学
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分子生物学
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遗传学
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植物生理学
✓ 已验证
DOI
PubMed
高影响因子
Cells
·
2022
·Vol. 11
·期 23
·3856
·
IF 7.666
Bin Liu,Hui Chen
The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) belong to the bHLH-PAS (basic Helix–Loop–Helix–Period/ARNT/Single-minded) family of transcription factors, which participate in the sensing and transmitting stimuli of exogenous and endogenous chemical substances, and subsequently activates genes transcription involved in various detoxification and physiological functions. However, they have not been identified inDendroctonus armandi,and their roles in the detoxification metabolism are unclear. In the present study,AhRandARNTofD. armandiwere characterized. Spatiotemporal expression profiling indicated thatDaAhRandDaARNTwere highly expressed in the adult and larval stages ofD. armandiand mainly expressed in the midgut and Malpighian tubules of adults. Additionally, the expression ofDaAhRandDaARNTsignificantly increased after exposure to (−)-?-pinene, (+)-3-carene, and (±)-limonene. SilencingDaAhRandDaARNTincreased the susceptibility ofD. armandito (−)-?-pinene, (+)-3-carene, and (±)-limonene, and the activities of detoxification enzyme were also remarkably reduced. Moreover,DaCYP6DF1andDaGSTs2were significantly down-regulated after injections ofdsAhRanddsARNTin the male and female adults, with the expression ofDaCYP6DF1decreasing by higher than 70%. The present study revealed that the transcription factorsAhRandARNTofD. armandiwere induced by terpenoids and participated in the regulation ofDaCYP6DF1 expression, which was associated withD. armandi’s susceptibility to (−)-?-pinene and (±)-limonene. These results may provide a theoretical basis for the integrated control ofD. armandiand improve our comprehension of insect toxicology.Keywords:Dendroctonus armandi;AhR;ARNT;detoxification metabolism;transcriptional regulation
研究领域:
昆虫学
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分子生物学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2022
·
IF 4.966
Bin Liu,Hui Chen
Bark beetles rely on detoxifying enzymes to resist the defensive oleoresin terpenes of the host tree. Insect cytochrome P450 (CYPs) plays a key role in the detoxification of plant allelochemicals and pesticides. CYP6 family is unique to Insecta, and its biochemical function is basically related to catabolize heterologous substances. In this study, two Dendroctonus armandi CYP6 genes, CYP6DF1 and CYP6DJ2, were characterized. Spatiotemporal expression profiling revealed that CYP6DF1 and CYP6DJ2 expressions were higher in larvae and adult stages of D. armandi than in egg and pupae stages, and that two genes predominantly expressed in brain , midgut, fat body, or Malpighian tubules . Moreover, CYP6DF1 and CYP6DJ2 expressions were significantly induced after exposure to (+)- α -pinene. Importantly, silencing CYP6DF1 and CYP6DJ2 significantly inhibited the CYP activity and increased the mortality in the adults fumigated with (+)- α -pinene. Additionally, piperonyl butoxide exposure to adults also increase the sensitivity after treatment with (+)- α -pinene, which led to a significant reduction of the CYP activity, resulting a significant increase in adult mortality. These results suggest that the CYP6 family plays a key role in determining the susceptibility of D. armandi to (+)- α -pinene, which may have implications for the development of novel therapeutics to control this important pest.
研究领域:
昆虫学
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生物化学
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分子生物学
·
害虫管理
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DOI
PubMed
高影响因子
MICROBIAL ECOLOGY
·
2023
·
IF 3.6
Ma Wenwen,Lin Lu,Peng Qiannan
Terrestrial microbial consortia were reported to play fundamental roles in the global carbon cycle and renewable energy production through the breakdown of complex organic carbon. However, we have a poor understanding of how biotic/abiotic factors combine to influence consortia assembly and lignocellulose degradation in aquatic ecosystems. In this study, we used 96 in situ lignocellulose enriched, coastal intertidal zone-derived bacterial consortia as the initial inoculating consortia and developed 384 cultured consortia under different lignocellulose substrates (aspen, pine, rice straw, and purified Norway spruce lignin) with gradients of salinity and temperature. As coastal consortia, salinity was the strongest driver for assembly, followed by Norway spruce lignin, temperature, and aspen. Moreover, a conceptual model was proposed to demonstrate different succession dynamics between consortia under herbaceous and woody lignocelluloses. The succession of consortium under Norway spruce lignin is greatly related with abiotic factors, while its substrate degradation is mostly correlated with biotic factors. A discrepant pattern was observed in the consortium under rice straw. Finally, we developed four groups of versatile, yet specific consortia. Our study not only reveals that coastal intertidal wetlands are important natural resources to enrich lignocellulolytic degrading consortia but also provides insights into the succession and ecological function of coastal consortium.
研究领域:
微生物学
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环境科学
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生物技术
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生态学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）