ANALYST
·
2015
·Vol. 140
·期 19
·6660-6670
·
IF 4.107
Qing Zheng,Hao Wu,Zongxu Shen,Wenyu Gao,Yu Yu,Yuehui Ma,Weijun Guang,Quangui Guo,Rui Yan,Junzhong Wang,Kejian Ding
A label-free electrochemical DNA sensor was fabricated by deposition of polyaniline and pristine graphene nanosheet (P/Gratios) composites in different mass ratios, DNA probe and bovine serum albumin (BSA) layer by layer on the surface of a glassy carbon electrode (GCE). Electrochemical impedance spectroscopy (EIS) was employed to monitor every step of fabrication of P/Gratio-based DNA sensors and to evaluate the detection results in terms of the hybridization of complementary DNA, mutant DNA and non-complementary DNA. The results illustrate that the P/Gratio-based DNA sensor could highly efficiently detect complementary DNA from 0.01 pm to 1 μm and discriminate single-nucleotide polymorphisms (SNPs). In the process of detection, double-stranded DNA (dsDNA), resulting from hybridization of a DNA probe, escaping from or remaining on the sensor surface, was monitored by changing the ratio of polyaniline (PANI) to graphene, which was decided by the competition between the electrostatic interaction and Brownian motion.
研究领域:
生物化学
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纳米材料科学
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分子诊断学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Zenghui Liu,Jinyan Xue,Luyao Chen,Lele Ma,Huaixia Yang,Yaping Zhang,Mingsan Miao
A new, simple and low-cost electrochemical analysis approach employing click polymerization as the signal amplification strategy was developed for the highly sensitive assay of aflatoxin B 1 (AFB 1 ). First, the aptamer served as the capture probe for AFB 1 self-assembled on the surface of the Au electrode via Au-S bonding. After complementary DNA (c-DNA) modified with azide groups was hybridized with the aptamer, a massive click polymer was introduced as a signal tag via click chemistry. Finally, the captured AFB 1 competed with the c-DNA to bind the aptamer and the c-DNA was shed from the electrode surface and the current intensity was reduced. The electrochemical aptamer sensor is easy to operate, selective and sensitive, and also exhibits excellent reliability in detecting AFB 1 in actual samples. A rapid, simple, efficient and sensitive platform for AFB 1 analysis is proposed in this work, and the strategy can be further extended to trace analysis of other analytes, safety detection, environmental monitoring and disease diagnosis after replacing the corresponding capture probes, with promising applications in the fields of food hygiene and safety.
研究领域:
分析化学
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生物化学
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食品安全
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环境监测
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DOI
高影响因子
Journal of Food Measurement and Characterization
·
2024
·
IF 2.9
Cheng Jun-Hu,Zhang Xinxue,Ma Ji,Sun Da-Wen
Tropomyosin (TM), the major allergen in shellfish, is a common protein cause of allergenic diseases, hence a sensitive, efficient method for TM detection is urgently needed to protect allergy sufferers in food products. In this work, a novel fluorescent aptasensor for TM-sensitive detection based on DNA-templated silver nanoclusters (AgNCs) and catalytic hairpin assembly (CHA) was designed. With the rapid separation and enrichment of magnetic beads, the detection of TM was converted to the detection of cDNA (complementary DNA). Then the cDNA was utilized as the initiator to successfully trigger the CHA amplification technique, which ultimately formed double-stranded to induce high fluorescence signals by AgNCs. Specifically, the cDNA could trigger repeated cycles, which enabled signal amplification and enhanced aptasensor performance with an excellent linear relationship in a wide linear range from 0.1 to 50 μg/mL (R 2 = 0.9967) and a desirable low detection limit of 0.0179 μg/mL. It is worth noting that the method was successfully validated with high specificity and stability for detecting processed shrimp products, implying great potential in the field of allergen assay.
研究领域:
食品科学
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分析化学
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生物传感器
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过敏测试
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DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 4
·1-8
·
IF 6.232
Zhao Jialin,Zhu Zhong-Zheng,Huang Xing,Hu Xiaojun,Chen Hongxia
A triple recognition voltammetric method for the determination of brain natriuretic peptide (BNP) is described. Gold nanoparticles (AuNPs) and magnetic nanoparticles (MagNPs), sized 26 and 310 nm, respectively, were synthesized and characterized by transmission electron microscopy (TEM), FT-IR, dynamic light scattering (DLS), and Z-potential measurements. Antibody-modified MagNPs and methylene blue–labeled aptamer (Apt-MB)–modified AuNPs were used as an identifier, a signal reporter, and an amplifier, respectively. In the presence of BNP, the magnetic gold nanocomposite is formed through cascade conjugation via specific interaction. It then hybridized with complementary DNA (cDNA) on the interface, thereby amplifying the current signal of Apt-MB and increasing the selectivity of the immunoassay. Results obtained demonstrate the development of a highly selective method with a detection limit of 0.56 pg mL −1 and a linear response over the concentration range 1–10,000 pg mL −1 . The standard deviation of the method is < 6% while the recovery ranged from 92.2 to 104.2%.
Schematic representation of triple recognition electrochemical immunosensor based on two functionalized nanoparticles (antibody-modified magnetic nanoparticle (MNP-Ab) and aptamer-modified gold nanoparticle (AuNPs-Apt)) for determination of brain natriuretic peptide (BNP).
研究领域:
纳米技术
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分析化学
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生物化学
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医学
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PubMed
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Nana Sun,Yuan Ding,Zhexuan Tao,Hongjie You,Xiude Hua,Minghua Wang
An upconversion fluorescence DNA probe which consists of aptamer-conjugated magnet nanoparticles (apt-MNPs) and complementary DNA-conjugated upconversion nanoparticles (cDNA-UCNPs) was developed to detect acetamiprid. Acetamiprid can specifically conjugate with the apt-MNPs to dissociate the cDNA-UCNPs from the apt-MNPs and resulted in reduced fluorescence intensity through an external magnet. The change of fluorescence intensity (△I) is positively related to the concentration of acetamiprid, which can be applied for the quantification of acetamiprid. Under optimal conditions, a linear detection range and detection limit are 0.89–114.18 μg/L and 0.65 μg/L, respectively. The probe was successfully used to detect acetamiprid in spiked paddy water, soil, pear, apple, wheat and cucumber. Average recoveries are 78.2%–103.5% with intra-day relative standard deviations (RSDs) of 2.6%–10.9% and inter-day RSDs of 4.3%–10.2%. The amounts of acetamiprid in the authentic paddy water and pear samples detected by the DNA probe are significantly correlated with that detected by high-performance liquid chromatography (HPLC).
研究领域:
分析化学
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环境科学
·
农业科学
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DOI
PubMed
高影响因子
JOURNAL OF ELECTRONIC MATERIALS
·
2025
·
IF 2.5
Qu Zhengquan,Zhang Baozhong,Liu Xihong,Cui Xiaoying,Zhao Ke,Li Ying,Xie Lingling,Zhu Huina,Chen Hanyu,He Baoshan
Fumonisin B1 (FB1) is a secondary metabolite generated by Fusarium verticillioides , which can widely contaminate crops. Herein, an electrochemical aptamer sensor with high sensitivity and selectivity was constructed for FB1 detection, leveraging the specific recognition capability of aptamers. Polyethylenimine-functionalized cerium dioxide-supported platinum nanoparticles (PEI-CeO 2 @PtNPs) were used for the modification of electrodes. The nanocomposite enhanced electrical conductivity on the electrode, and provided more attachment sites to increase the loading capacity of aptamer chains. Additionally, polyethylenimine-functionalized melamine-cyanuric acid condensation polymers (PEI-MCA) were used as carrier for the signal probe. Because of the unique porous structure and abundant functional groups on the surface of MCA, the loading efficiency of methylene blue (MB) was significantly enhanced, and the complementary DNA (cDNA) strand of aptamers was effectively loaded on PEI-MCA by strong electrostatic interaction to construct signal probes. The cDNA was captured by aptamers through complementary base pairing, and PEI-CeO 2 @PtNPs amplified electrical responses cooperatively with PEI-MCA. In the presence of FB1, the aptamers preferentially bound to FB1, and the signal probes were detached from the electrode surface, resulting in a decrease in the electrochemical response. Under optimal conditions, the dynamic range of the constructed aptasensor was 1 × 10 −1 to 1 × 10 4 pg/mL, with a detection limit of 89 fg/mL. Additionally, the sensor had high selectivity, reproducibility, and stability, and could detect FB1 in milk and corn flour samples.
研究领域:
分析化学
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食品安全
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生物传感器
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纳米材料
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电化学
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DOI
高影响因子
TALANTA
·
2023
·
IF 6.1
Nuo Duan,Yuting Chang,Wenhui Lv,Changxin Li,Chunxia Lu,Zhouping Wang,Shijia Wu
Herein, a SiO 2 @Ag NPs core/shell nanoparticles were synthesized to fabricate a surface-enhanced Raman spectroscopy (SERS) sensor for the simultaneous determination of histamine (HIS) and tyramine (TYR) based on specific aptamer recognition and ratiometric strategy. SiO 2 @Ag NPs with 4-thiosaminophenol (4-ATP) and Nile blue A (NBA) molecules were used as an internal standard (IS) and labeled with aptamers corresponding to HIS and TYR, respectively. Raman reporter molecules ROX and Cy5 labeled complementary DNA (cDNA) were then hybridized with aptamers to form rigid double-stranded DNA. After the HIS and TYR were captured by their aptamers, resulting in the dissociation of cDNA and separated from the SERS substrate. Therefore, the SERS signal intensity at 1503 cm −1 of ROX and 1358 cm −1 of Cy5 tagged on the terminal of cDNA decreased with the concentration of HIS and TYR increasing, while the SERS signal intensity at 1079 cm −1 of 4-APT and 592 cm −1 of NBA on the substrate remain stable. Thus, the concentrations of HIS and TYR can be determined by the I 1503 /I 1079 and I 1358 /I 592 values, respectively. This sensing strategy achieves a lower detection limit of 0.2 ng/mL for HIS and 0.05 ng/mL for TYR, respectively, demonstrating promising applications in sensitive detection of BAs in animal-derived foodstuff.
研究领域:
分析化学
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食品科学
·
生物技术
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DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Nuo Duan,Tangbin Yao,Changxin Li,Zhouping Wang,Shijia Wu
We designed a SERS-based method to detect β-lactoglobulin (β-Lg), a major allergen of milk, utilizing aptamers to specifically anchor β-Lg. The Au–Ag nanourchins with excellent Raman enhancement effects were prepared and modified with a β-Lg aptamer as the capture substrate. By taking advantage of the hybridization of aptamer and Raman reporter molecule (6-Carboxyl-X-Rhodamine: ROX)-labeled complementary DNA (cDNA), when ROX is close to Au–Ag nanourchins, the SERS signal is enhanced. In the presence of β-Lg, aptamer binding to β-Lg induces the dissociation of ROX-cDNA from Au–Ag nanourchins, thereby decreasing the SERS signal. Under optimal conditions, the present bioassay provides a wide detection range of 10 ng/mL to 1000 ng/mL with a detection limit of 0.07 ng/mL. In addition, the established SERS sensor can selectively and accurately identify β-Lg with recovery rates of 93.25–107.0% in milk samples. It provides great potential as a sensitive and reliable detection method against the growing threat of allergens.
研究领域:
分析化学
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食品科学
·
纳米技术
·
过敏原检测
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Xinyue Wang,Chen Chen,Geoffrey I.N. Waterhouse,Xuguang Qiao,Yufeng Sun,Zhixiang Xu
Herein, a novel surface enhanced Raman spectroscopy (SERS) aptasensor was developed for amantadine (AMD) detection, based on magnetite nanoparticles coated with polyethylenimine, silver nanoclusters and aptamers (Fe 3 O 4 @PEI@AgNC-apt) as the capture probe and complementary DNA-modified gold nanorods (AuNRs@4-MPBA@Ag-c-DNA containing 4-mercaptophenylboric acid molecules) as the reporter probe. In the presence of AMD, the AMD and the reporter probe competed for the aptamer on the surface of the capture probe, resulting in the reporter probe detaching from the capture probe leading to a decrease in intensity of the SERS signal at 1067 cm −1 for 4-MPBA. Under optimal conditions, a good linear relationship was established between the SERS intensity at 1067 cm −1 and the logarithm of the AMD concentration over the range 10 −6 -10 2 mg L −1 , with a LOD of 0.50 × 10 −6 mg L −1 . The AMD levels in spiked samples were evaluated using the SERS aptasensor, with good recoveries ranging from 90.57% to 113.49% being obtained.
研究领域:
分析化学
·
纳米技术
·
食品科学
·
光谱学
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DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2019
·
IF 1.248
Chunyan Wang,Hui Zhang,Xiaoqing Jiang,Bo Zhou
A sensitive electrochemical biosensor was designed for determination of aflatoxin B1 (AFB1) using a copper-based metal-organic framework (Cu-MOF), which has strong electrochemical activity and exonuclease III (Exo III)-assisted recycling for dual signal amplification. Hairpin DNA (S1) was immobilized on the electrode. The AFB1 was recognized by aptamer DNA (S2) and complementary DNA (S3) was released. The S3 hybridized with the hairpin S1 to form the Exo III hydrolyzed double-stranded DNA, leaving a partial sequence of hairpin DNA (S1′) on the electrode and releasing S3 for the next cycle of the opening and digestion of hairpin S1. The amplified S1′ then was able to combine with more signal probes. Cu-MOF bond gold nanoparticles (AuNPs) by -NH2 were immobilized to capture DNA (S4) to obtain Cu-MOF/AuNPs/S4. This signal probe Cu-MOF/AuNPs/S4 was able to hybridize with the electrode and generate an amplified electrochemical signal. Under the optimized conditions, this electrochemical biosensor for AFB1 exhibited a low detection limit of 6.7 × 10−7 ng/mL at a signal-to-noise equal to 3 and a wide linear range from 10−6 to 1 ng/mL. The biosensor was also used to analyze AFB1-spiked beer sample with recovery values between 96% and 103%. This method has the potential to become a valuable technology for detecting various toxins by the selection of the appropriate aptamer DNA.
研究领域:
分析化学
·
生物技术
·
食品安全
·
环境科学
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Lining Su,Guizhen Wang,Linlin Zhao,Yuan Deng,Yujing Guo,Yong Xiao,Huanwen Wang,Chuan Dong,Lifang Fan
Due to the urgent need for detecting trace amounts of 3,3′,4,4′-tetrachlorobiphenyl (PCB77) in the environment, we have developed an efficient and visible-driven photoelectrochemical (PEC) sensing platform based on carbon quantum dots (CQDs) modified titanium dioxide nanorods (TiO 2 NRs), coupling with exonuclease I (Exo I) assisted in target recycling for significant signal amplification. CQDs/TiO 2 NRs with high visible-light absorption ability and electron-hole separation efficiency is used as photoactive substrate for anchoring anti-PCB77 aptamer and its complementary DNA (cDNA). With the addition of PCB77, the specific interaction between PCB77 and its aptamer forces aptamer to separate from the electrode surface, resulting in an increase in photocurrent density. Adding Exo I in the test system, a self-catalytic target cycle was motivated, which significantly increased the PEC signal by more than twice, achieving signal amplification. The relationship between the photocurrent density changes and the concentrations of PCB77 are utilized to achieve quantitative detection of PCB77. The designed PEC sensing platform has good analytical performance with a detection limit as low as 0.33 pg L −1 , high selectivity and stability. Moreover, the PEC sensor is successfully used to evaluate the content of PBC77 in the environment samples. The established sensing platform provides a simple and efficient method for detecting trace amounts of PCB77 in the environment.
研究领域:
环境科学
·
分析化学
·
光电化学
·
纳米技术
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Ying Zhu,Xiao-chun Hu,Shuo Shi,Ru-ru Gao,Hai-liang Huang,Yan-yan Zhu,Xiao-yan Lv,Tian-ming Yao
We report here an ultrasensitive strategy based on the recognition-induced conformational alteration of aptamer and fluorescence turn-on abilities of guanine-rich (G-rich) DNA sequence in proximity to silver nanoclusters for adenosine triphosphate (ATP), adenosine (A) and thrombin (TB) detection. Herein, we designed two tailored DNA sequences noted as complementary DNA (abbreviated as c-DNA) and signal probe DNA (abbreviated as s-DNA), respectively. c-DNA is designed as a special structure consisting of a sequence complementary to aptamer at the 3′-end and a guanine-rich DNA sequence at the 5′-end; s-DNA contains a cytosine-rich sequence responsible for Ag NCs templated synthesis at the 3′-end and a link sequence (part of aptamer) complementary to partial of the c-DNA at the 5′-end. In the presence of target, the aptamer associated with the target, resulting in the formation of duplex DNA (dsDNA), the DNA–Ag NCs thereafter could close to the guanine-rich sequence, leading to enhanced fluorescence signal readout. The widespread application of the sensing system is achieved success in the detection of three biomolecules . ATP, adenosine and thrombin in the range of 0.5-8.0 μM, 0.5-7.0 μM and 50-900 nM could be linearly detected with the detection limits of 91.6 nM, 103.4 nM and 8.4 nM, respectively. This label-free and turn-on fluorescent sensing system employing the mechanism proposed here turns out to be sensitive, selective, and convenient for the detection of biomolecules without washing and separation steps.
研究领域:
分析化学
·
生物传感器
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纳米技术
·
生物化学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）