FITOTERAPIA
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2012
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IF 1.848
Cong Liu,Yun-Feng Cao,Zhong-Ze Fang,Yan-Yan Zhang,Cui-Min Hu,Xiao-Yu Sun,Ting Huang,Jia Zeng,Xu-Ran Fan,Mo Hong
Deoxyschizandrin and schisantherin A are major bioactive lignans isolated from Fructus schisandrae which has been widely used as a tonic in traditional Chinese medicine for many years. Inhibition of UDP-glucuronosyltransferases (UGTs) by herbal components might be an important reason for clinical herb–drug interaction. The aim of the present study is to investigate the inhibitory effect of deoxyschizandrin and schisantherin A on major UGT isoforms. Recombinant UGT isoforms were used as enzyme source, and a nonspecific substrate 4-methylumbelliferone (4-MU) was utilized as substrate. The results showed that 100 μM of deoxyschizandrin and schisantherin A exhibited strong inhibition on UGT1A3, and negligible inhibition on other tested UGT isoforms. Furthermore, deoxyschizandrin and schisantherin A were demonstrated to inhibit UGT1A3 in a concentration-dependent manner, with IC 50 value of 10.8 ± 0.4 μM and 12.5 ± 0.5 μM, respectively. Dixon and Lineweaver–Burk plots showed that inhibition of UGT1A3 by deoxyschizandrin was best fit to competitive inhibition type, and inhibition kinetic parameter (K i ) was calculated to be 0.48 μM. Inhibition of UGT1A3 by schisantherin A gave the best fit for types of noncompetitive inhibition, and the results showed K i to be 11.3 μM. All these experimental data suggested that herb–drug interaction might occur when deoxyschizandrin or schisantherin A containing herbs were co-administered with drugs which mainly undergo UGT1A3-mediated metabolism. However, given that many in vivo factors could influence the in vitro – in vivo extrapolation (IVIVE), these in vitro inhibitory parameters should be considered with caution.
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DOI
PubMed
高影响因子
Waste and Biomass Valorization
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2024
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IF 2.6
Zhao Zijia,Gao Yan,Yuan Min,Ye Cai,Guo Yingxue,Zhao Yanli,Li Jinlian,Cui Jiwen,Dong Tianwei,Wu Dongmei
A significant quantity of residues will produce when volatile oil in Schisandra chinensis fruit is extracted by steam distillation. It is important to investigate the residues for potential use. Currently, there is an increasing demand for anti-photoaging products due to the skin damage caused by UV radiation. In this paper, the ingredients of the residues were investigated, and the anti-photoaging activities of residues extracts were studied. The results showed that the residues still contained notable amounts of lignans, flavonoids, and organic acids. In the extracted fraction of dichloromethane (DE), the residues contained higher amounts of schizandrol B and schisantherin A compared to the fresh fruit. The levels were particularly high at 17.39 ± 0.02 and 52.58 ± 0.04 mg/ml, respectively. By lowering the amount of matrix metalloproteinase-1 (MMP-1), reactive oxygen species (ROS) accumulation, and type I collagen (COL-I) loss in fibroblasts, the residues showed targeted benefits in the fight against skin photoaging. In particular, the DE-R phase of residues was very successful in raising the level of COL-I, matching or even exceeding that of retinol (ROL) and undistilled fresh fruits. Protocatechuic acid, schizandrol A, schizandrol B, schisantherinA, schisandrin A and schizandrin B were found to have a significant correlation with the anti-photoaging ability of residues. Particularly, there was a strong and significant correlation between an increase in COL-I and schizandrol B (|ρ| = 0.829*) and schisantherin A (|ρ| = 0.928**), and the distillation procedure has the potential to increase the amount of schizandrol B and schisantherin A in the residues. Graphical
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DOI
高影响因子
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
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2016
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IF 2.217
Xiao-Hua Zhang,Hai-Long Wu,Xiao-Li Yin,Yong Li,Xiang-Dong Qing,Hui-Wen Gu,Chao Kang,Ru-Qin Yu
In this paper, a new strategy of introducing an extra solvent mode in the three-way EEMs data to construct a four-way EX–EM-solvent-samples data set was used in order to investigate the ‘third-order advantage’ by comparing the performances of two four-way calibration algorithms with those of two three-way calibration algorithms. The results of the self-weighted alternating normalized residue fitting (SWANRF) and the parallel factor analysis (PARAFAC) algorithms were both disappointing in this work due to serious collinearity and high background interference. In contrast, the satisfactory results (average recovery, 100.0–107.4% and standard deviation, 1.0–3.0%) were obtained by the third-order calibration methods based on both four-way self-weighted alternating normalized residue fitting (four-way SWANRF) and four-way parallel factor analysis (four-way PARAFAC) algorithms. In addition, for prediction of Dulbecco's modified eagle medium (DMEM) samples, the root-mean-square error of prediction (RMSEP) values for schizandrol B obtained from two three-way calibration algorithms were 60.91 and 35.46 μg mL − 1 with 80% ethanol in the aqueous solvent , which were obviously much larger than these (0.0832 and 0.0825 μg mL − 1 ) obtained using two algorithms of the four-way calibration. The results demonstrated that third-order calibration can extract more inherent information from the data, and can easily solve the problems of serious collinearity and high background interference. In short, among the advantages of this method over the existing methods, low cost, non-toxic and non-destructive analysis can be cited.
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DOI
高影响因子
危险品化学品经营许可证（带存储）