In order to understand the significant differences in activities and effects between Asian ginseng (ASG) and American ginseng (AMG), it is important to study the correlation between chemical structures and biological activities in the two types of herbs. However, more attention has been paid to the analysis of ginsenosides in previous reports distinguishing between ASG and AMG. There are some other bioactive compounds besides ginsenosides, however, few studies have focused on a systematic comparison of these types of compounds. Two metabolomic methods were developed in this study by qualitative data acquisition using normal phase liquid chromatography mass spectrometry (NPLC-MS) and reverse phase liquid chromatography mass spectrometry (RPLC-MS) respectively, in combination with principal component analysis (PCA). Results show that both NPLC-MS and RPLC-MS-based metabolomic methods are feasible in composition profiling, biomarker screening as well as in discrimination of ASG and AMG. 17 ginsenosides were identified as analytical markers in RPLC-MS-based metabolomic method. In comparison, using NPLC-MS-based method, 5 ginsonosides, two amino acids as well as 1 oligosaccharide were identified as analytical markers. Therefore, RPLC-MS-based metabolomic method exhibits better profiling in ginsenosides, while NPLC-MS-based metabolomic method offers the advantage that multiple active ingredients can be determined simultaneously. The two methods are both helpful in identification of biomarker as well as in discrimination of American ginsengs from Asian ginsengs.
Yuanqing Luo,Xiaoyong Zhao,Zhan Gao,Huiwen Wang,Yaqing Liu,Cheng Guo,Yuanjiang Pan
Oligosaccharides play a key role in many biological functions, and the accurate identification of oligosaccharide structures is an important prerequisite for a comprehensive understanding of the biological functions of oligosaccharides. MALDI-TOF/TOF tandem mass spectrometry has been considered as a potential technique for the structural characterization of oligosaccharides. In this work, palladium nanoparticles decorated thiol-functionalized metal organic framework nanocomposite (UiO-66-(SH) 2 @Pd NPs) was fabricated as an efficient matrix to assist laser desorption/ionization mass spectrometry (LDI-MS) for oligosaccharides analysis. The ionization efficiency of oligosaccharides was significantly improved owning to the synergistic effect of MOF and Pd nanoparticles, which is favorable for further oligosaccharide structure identification. By combining LDI-LIFT-TOF/TOF, 24 oligosaccharide isomers including disaccharides, trisaccharides and tetrasaccharides, were effectively distinguished. In addition, the relative quantification curves for isomeric oligosaccharides were established with good linear correlations. The method was successfully applied to the identification and quantification of sucrose and maltose in three batches of Asian ginseng and American ginseng respectively, showing potentiality of MOF materials and metal nanomaterials assisted structural analysis of oligosaccharide isomers.
危险品化学品经营许可证（带存储）