TOXICOLOGY AND APPLIED PHARMACOLOGY
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2022
·
IF 4.46
Suizhi Cheng,Li Che,Qingqing Yang,Rongjing Sun,Yuhong Nie,Haoyue Shi,Yiwen Ding,Li Wang,Zuo Du,Zhenzhong Liu
N -methyl- N′ -nitro- N -nitrosoguanidine (MNNG) is a common alkylating agent , which can be experimentally used as a chemical mutagen and carcinogen, extensively existing in the environment. Folic acid (FA), part of the B group of vitamins, plays an important role in defending against inflammation and reducing the risk of cancers. Nevertheless, there is little research on the protective effects of FA against MNNG-induced esophageal inflammation, and its underlying mechanism still remains elusive. Hence, in the present study, we exposed MNNG to SD rats and esophageal cells to establish the esophageal inflammation models. Our research aims to explore the protective roles of FA against esophageal inflammation induced by MNNG via NF-κB pathway by CCK-8, EdU, RT-qPCR, ELISA , H&E, Western blot . Our results revealed that MNNG decreased the viability of esophageal cells, which was restored under FA intervention. Besides, FA relieved the elevation of IL-6, IL-8 and TNF-α in MNNG-induced esophageal inflammation. Moreover, histopathological analysis showed that epithelial spinous cells proliferated in mucous layer, and inflammatory cells were locally infiltrated in the submucosa after MNNG exposure, while the pathological damage of esophageal tissues was gradually alleviated along with increasing FA doses. And Western blot results demonstrated that FA could relieve the rise of phosphorylated IκBα (p-IκBα) and phosphorylated p65 (p-p65) proteins induced by MNNG. Therefore, it is reasonable to believe that FA has a crucial role in preventing MNNG-induced esophageal inflammation through inhibiting the NF-κB pathway, thereby down-regulating the expressions of IL-6, IL-8 and TNF-α.
研究领域:
药理学
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毒理学
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分子生物学
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病理学
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产品关联
DOI
PubMed
高影响因子
ANNALS OF MICROBIOLOGY
·
2017
·Vol. 68
·期 1
·27-34
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IF 1.122
Xiao Yaqing,Li Peijun,Xu Mei,Wang Wu,Chen Conggui
The objective of this study was to evaluate the ability of five strains of meat-borne bacteria to decrease N-nitrosodimethylamine (NDMA) and N-nitrosodiethylamine (NDEA) and to elucidate the mechanism in Mann-Rogosa-Sharp (MRS) broth. Lactobacillus pentosus R3 was found to be the most effective in decreasing the concentration of the two N-nitrosamines (NAs) in MRS broth, with a rate of 22.05% for NDMA and 23.31% for NDEA. The concentration of the two NAs could not be reduced by either extracellular metabolites or intracellular extracts of Lb. pentosus R3 (P > 0.05), and proteinaceous substances in the cell debris were found to be responsible for the decrease. These were surface-layer proteins (SLPs) located on the cell wall. Therefore, the decrease in NDMA and NDEA by Lb. pentosus R3 is associated with its SLPs. Lb. pentosus R3 may be developed as a starter culture in the production of fermented foods with lower NAs.
研究领域:
食品科学
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微生物学
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毒理学
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产品关联
DOI
高影响因子
ACS Applied Bio Materials
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2020
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IF 0
Jie Zhang,Jianfei Kan,Yanyan Sun,Miae Won,Ji Hyeon Kim,Weifen Zhang,Jin Zhou,Zhaosheng Qian,Jong Seung Kim
Peroxynitrite (ONOO–), a powerful biological oxidant, is produced in the mitochondria and reacts with many biomolecular targets under various pathological conditions, leading to a range of disease states. In this work, we developed a nanoliposome-encapsulated ratiometrically fluorescent probe (NRF) based on a hemicyanine structure Cy-O obtained by facile synthesis. Upon reaction with ONOO–, the oxidation and hydrolysis of a π-conjugation system within the nanoliposome triggers a ratiometrically fluorescent response and a large-scale emission shift (238 nm), which provides a specific and sensitive means for the ONOO– detection. Moreover, we have performed DFT calculation at the 6-31+G(d,p) level using a suite of Gaussian 09 programs to obtain insights into the chemical structure optical properties of Cy-O. In addition, the practical applications of the nanoprobe to image exogenous and endogenous ONOO– were achieved further in live cells and animals triumphantly.
研究领域:
分析化学
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生物化学
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分子生物学
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材料科学
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产品关联
DOI
PubMed
Journal of Cleaner Production
·
2017
·
IF 5.715
Yuanan Hu,Yuanzhen He,Hefa Cheng
N -nitrosodimethylamine (NDMA), a highly toxic and carcinogenic disinfection byproduct , cannot be efficiently removed by conventional water treatment processes , while sorption by microporous minerals, followed by destruction with microwave irradiation has been developed as a promising alternative. This work examined the impact of surface cation density and type on sorption of NDMA on dealuminated ZSM-5 zeolites , and on microwave-induced degradation of the sorbed NDMA, as well as the non-thermal effect of microwave. NDMA sorption was found to increase with the framework Si/Al ratio of dealuminated ZSM-5 zeolites (i.e., decreasing density of surface cations), and was also influenced by the type of surface cations. Degradation of the sorbed NDMA proceeded faster with increases in the power level of microwave and in the micropores with higher densities of surface cations, which was attributed to the increases in the number and/or temperature of micro-scale “hot spots” formed by microwave dielectric superheating of the surface cations. The degradation rate of sorbed NDMA was also found to be inversely correlated with the hydration free energy of surface cations present in the zeolite micropores . The apparent activation energy estimated from the temperature dependence of NDMA degradation in the micropores under microwave irradiation was 18.2 kJ/mol, which is much lower than those of typical thermolysis reactions and is indicative of significant contribution from the non-thermal effect of microwave.
研究领域:
环境科学
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化学工程
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2017
·
IF 7.78
Jiali Zhu,Shan Sun,Kai Jiang,Yuhui Wang,Wenqing Liu,Hengwei Lin
Herein, a highly sensitive and selective fluorimetric nanoprobe for peroxynitrite (ONOO − ) detection based on photoinduced electron transfer (PET) from ferrocene (Fc) to carbon dots (CDs) is reported. The nanoprobe (named CDs-Fc) can be facilely constructed through covalently conjugating CDs and ferrocenecarboxylic acid . Further studies reveal that the energy level of highest occupied molecular orbital (HOMO) of the CDs is lowered with the addition of ONOO − due to its oxidation and nitration capabilities. Thus, an efficient electron transfer from Fc to the excited states of CDs could occur, leading to obvious fluorescence quenching . The fluorescence quenching of the nanoprobe was determined to be peroxynitrite concentrations dependence with a linear range between 4 nM to 0.12 μM. Thanks to the excellent optical properties of the CDs and efficient electron transfer efficiency from Fc to the excited CDs, the nanoprobe exhibits very high sensitivity to ONOO − with a limit of detection (LOD) of 2.9 nM. To the best of our knowledge, this LOD is the highest reported value till today for the detection of peroxynitrite. Besides, the nanoprobe also shows excellent selectivity to ONOO − among a broad range of substances, even including other reactive oxygen/nitrogen species (ROS/RNS). Finally, the nanoprobe was verified to be very low cytotoxicity, and was successfully applied for intracellular ONOO − detection. This work would provide a promising tool for the research of ONOO − in cytobiology and disease diagnosis.
研究领域:
分析化学
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生物医学
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DOI
高影响因子
Carbon Letters
·
2024
·Vol. 34
·期 7
·1927-1939
·
IF 5.5
Ma Liyan,Sun Fengyi,Jiang Zhuwu,Di Hongcheng,Pan Chuntao,Zhang Fengying,Bai Xue,Zhang Hongyu
Activated carbon has broad application prospects for treating pollutants due to its easy availability, low cost and good adsorption. In our work, nano-activated carbons (NAC) with abundant functional groups are obtained by the oxidation modification of HNO 3 , (NH 4 ) 2 S 2 O 8 , and KMnO 4 , which are used to construct the particle electrodes to degrade NDEA in a continuous flow electrochemical reactor, and the influence of relevant factors on the performance of NDEA removal is discussed. The experimental data show that the optimal degradation efficiency is 42.55% at the conditions of 3 mL/min influent water flow, 0.21 M electrolyte concentration, 10 mA/cm 2 current density, and 10 μg/mL initial NDEA concentration. The degradation of NDEA conforms to a quasi second order kinetic equation. The electrocatalytic mechanism of NAC electrodes for removing NDEA is firstly discussed. The effects of different free radicals on the degradation of NDEA are also demonstrated through free radical quenching experiments, indicating that the degradation of NDEA is dominated by ⋅OH. The degradation pathway of NDEA and final products are obtained using GC–MS. NAC particle electrodes as the cheap and efficient electrocatalyst in continuous flow electrochemical reactor system provide a greener solution for the removal of disinfection by-products from drinking water.
研究领域:
环境科学
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电化学工程
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2022
·Vol. 23
·期 22
·14337
·
IF 6.208
Xun Zhang,Yaxi Wang,Xinyuan Zhao,Chunlin He,Siping Pang
Oxygen balance and heat of formation are closely related to the nitrogen and oxygen content in a molecule and have a significant effect on the detonation performance of energetic materials. Here a new family of 1,2,4-triazolo [4,3-b][1,2,4,5]-tetrazine containing gem-dinitromethyl and nitroamine with high nitrogen-oxygen content was synthesized and characterized. Moreover, the structure of the guanidine salt (3) and TATOT salt (4) were confirmed by single-crystal X-ray diffraction. The nitrogen and oxygen content of ammonium salt2reached 82.5%, with a high density (1.805 g cm−3) and high detonation properties (D= 8900 m s−1;P= 32.4 GPa), which were similar to those of RDX.Keywords:nitroamine;gem-dinitromethyl;high nitrogen-oxygen content;triazolo-tetrazine fused ring
研究领域:
化学
·
材料科学
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DOI
PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2014
·
IF 5.039
Cuiping Chen,Wenjun Zhou,Daohui Lin
N-nitrosodimethylamine (NDMA) is an emerging carcinogenic disinfection by-product in water environment. Biochars utilized as sorbents for the removal of NDMA from aqueous solution, and the sorption characteristics and mechanism were investigate. Biochars were prepared from bamboo, rice straw and wood sawdust at 300–700 °C. The pseudo-second-order model provided the best fit for the sorption kinetics of NDMA onto biochar and the sorption isotherms were described best with the Slips model. Biochar produced at 500 °C removed NDMA from aqueous solution relatively efficiently, and the bamboo char demonstrated the best sorption potential among the three types of biochars. Solution chemistry such as pH and metal ions did not show obvious effect on NDMA removal. Three sorption mechanisms are suggested for NDMA sorption onto biochars, H-bond attraction between N O and the O-containing moieties, hydrophobic force between CH 3 and the ordered graphitic structure, and partition process of NDMA into the non-carbonization part of biochar.
研究领域:
环境科学
·
化学工程
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DOI
PubMed
高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
·
2025
·
IF 8.2
Yuejie Zhang,Jingfeng Zhang,Weiwei Tang,Bin Li
Peroxynitrite (ONOO - ) is a highly reactive oxidant formed by the reaction of nitric oxide with superoxide. Excessive ONOO - can be produced by the body in response to multiple diseases, resulting in cell death through oxidation and nitration processes. However, technical challenges arise in detection due to its high reactivity and very short lifespan. In this work, we rationally designed and synthesized a novel tetrazine-hemicyanine-based probe (TZN-HCY). This probe demonstrates the ability to capture ONOO - with high selectivity and sensitivity, enabling the detection and imaging of ONOO - in biological samples with both fluorescence and MALDI MS. The 3,6-disubstituted tetrazine moiety could react with ONOO - , and the hemicyanine skeleton with a permanent positive charge could enhance MALDI MS detection. The responsive performances of the TZN-HCY probe were verified on cell models and livers of hepatic ischemia-reperfusion injury (HIRI) model mice. Due to the applicability to dual-mode imaging, the formation of ONOO - and its content change in the liver of living mice were visualized by fluorescence imaging, while the fine-scale spatial distribution of ONOO - in liver tissues was revealed by MALDI MS imaging. This dual-modal probe could serve as a powerful tool for elucidating the diverse and complex roles of biogenic ONOO - in ischemia-reperfusion injury.
研究领域:
生物化学
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医学影像学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
aBIOTECH
·
2025
·
IF 5
Zhang Wenyang,Wang Yanjiao,Li Wenyan,Wu Shaowen,Chen Yuanyuan,Ye Mingyang,Huang Wenjie,Fernie Alisdair R.,Yan Shijuan
Protein post-translational modifications such as phosphorylation and S -nitrosylation regulate protein functions and cellular programs in eukaryotes. Moreover, extensive evidence suggests crosstalk between these modifications. However, we lack a comprehensive method for the simultaneous detection and analysis of multiple post-translational modifications. Here, we present an optimized workflow that identifies phosphorylation and S -nitrosylation sites using a novel phosphate affinity tag switch technique. Validation with model proteins and complex biological samples confirmed the high sensitivity, coverage, and reproducibility of this method. Applying this method to Arabidopsis thaliana seedlings revealed 12,552 phosphorylation sites and 6,108 S -nitrosylation sites, representing the largest single-study dataset of S -nitrosylation sites to date. This approach enhances our understanding of post-translational modification dynamics in plant signaling, stress responses, and metabolism.
研究领域:
植物生物学
·
生物化学
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分子生物学
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蛋白质组学
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产品关联
DOI
PubMed
高影响因子
Journal of Engineered Fibers and Fabrics
·
2018
·
IF 0.678
Setara Begum,Jindan Wu,Carrington M. Takawira,Jiping Wang
Subtilisin treatment is feasible in benign conditions, and yields a clean, dynamic approach for Polyamide 6,6 (PA 6,6) modification. In this study, the water contact angle (WCA) method, capillary height and moisture regain were used measure hydrophilicity. To characterization the modification, the scanning electron microscope (SEM), amino group quantification and x-ray photoelectron spectrometry (XPS) were used. Fabric burst strength and hand properties were characterized. Optimum conditions for subtilisin treatment were found to be 55oC, neutral pH with 10% (owf) enzyme concentration for a fixed 90 min treatment period. X-ray photoelectron spectrometry (XPS) results showed that carboxyl groups were produced on the modified fiber surface. The modified fibers showed much improved hydrophilicity as indicated by reduced wicking time, improved capillary height and moisture regain percentage. The creation of carboxylic acid group sand increased number of amino groups detected on the fabric surface may positively influence hydrophilicity. In comparison to harsh alkali modification, the enzyme treatment led to smaller weight loss. In general, enzymatic treatments- a more eco-friendly alternative to alkaline treatments, resulted in a significant improvement in surface properties of PA 6,6 fabrics.
研究领域:
纺织科学
·
生物化学
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Huaqin Jiang,Lingling Shi,Wenxiang Wang,Yang Yang,Min Fang,Weiju Zhu,Cun Li
The peroxynitrite anion (ONOO - ) and viscosity play crucial roles in both physiological and pathological processes. In this study, we developed a bifunctional fluorescent probe MAR with an aggregation-induced emission (AIE) feature for detecting viscosity and ONOO - across different channels. MAR exhibited significant fluorescence enhancement to viscosity with a red emission at 600 nm and a good linear relationship in the range of 11.6 to 956 cP. Additionally, MAR could rapidly recognize and quantitatively respond to ONOO - with high selectivity, good sensitivity, a low detection limit (LOD = 6.97 nM), and a wide range of pH value (4.0 - 10.0), accompanied by visible blue fluorescence emission at 408 nm. The considerable wavelength separation (Δλ = 192 nm) between the maximum emission peaks of MAR for viscosity and ONOO - ensured minimal signal crosstalk in bioimaging. Cell imaging experiments indicated that MAR could target intracellular lipid droplets and respond to the changes of viscosity and ONOO - through independent red and blue channels. These results indicated that the AIEgen fluorescence sensor MAR was a promising candidate for detecting ONOO - levels and viscosity changes in live cells.
研究领域:
生物化学
·
医学检测
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）