Analytical Methods
·
2016
·Vol. 8
·期 17
·3578-3586
·
IF 1.915
Haijing Du,Yanxin Chu,Hong Yang,Kang Zhao,Jianguo Li,Pei She,Xiang Zhang,Anping Deng
Brombuterol, a new β-adrenergic agonist which can enhance the lean meat-to-fat ratio, is forbidden as an additive in animal feeds for livestock production due to its adverse effects on consumers. In this study, a highly sensitive and specific competitive enzyme-linked immunosorbent assay (ELISA) for the detection of brombuterol in tissue and feed samples was developed. The immunogen and coating antigen were prepared by directly linking brombuterol to carrier proteins using the diazobenzidine method. The antisera against brombuterol were obtained from immunized rabbits. The IC50 and limit of detection (LOD) values of the superior ELISA were 0.165 ng mL−1 and 0.007 ng mL−1, respectively. The cross-reactivity (CR) values of ELISA with clenbuterol, terbutaline and cimbuterol were less than 6.2%; there was no CR of ELISA with nine other compounds. To investigate the accuracy and precision of the assay, liver, meat and feed samples were spiked with brombuterol at different concentrations and analyzed by ELISA. Acceptable recovery rates of 91.9–115.4% and intra-assay coefficients of variation of 1.5–9.5% (n = 3) were achieved. The ELISA was also validated by HPLC. As revealed, both methods were highly correlated (R2 = 0.9927–0.9994, n = 6). The proposed ELISA was proven to be a feasible method for quantitative analysis of brombuterol in tissue and feed samples.
研究领域:
食品安全
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Ying-yue Zhang,Mei-juan Zhao,Chun-yao Liu,Kai Ma,Tian-yu Liu,Fei Chen,Li-na Wu,De-jun Hu,Guang-ping Lv
Immunoassay-based techniques are important on-site screening tools for the detection of mycotoxins in cereals. This study aims to evaluate the trueness, precision, repeatability and cross-reactivity of commercially available test strips, ELISA kits and UHPLC-MS/MS on analyzing zearalenone, ochratoxin A, deoxynivalenol, T-2 toxin and fumonisin B 1 . The results showed that false negative rate (25.7 %–37.4 %) of all tested mycotoxins by test strips was higher than the false positive rate (0 %–31.0 %). The repeatability of ELISA kits at the declared LOD dispersed from −85.7 % to +98.4 %. ELISA kits were more accurate at 50 % of the maximum residue limit (MRL) of mycotoxins than 150 % and 200 %. All the tested deoxynivalenol/zearalenone derivatives had cross-reactivity with different level, and sample matrix could reinforce this overestimation of target mycotoxin. This study emphasized that higher-quality antibody screening and more analytical performance investigations are need to address for on-site detection of mycotoxins in the future.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2015
·
IF 0.986
Naifeng Xu,Liqiang Li,Shanshan Song,Liguang Xu,Hua Kuang,Chuanlai Xu
We developed a sensitive lateral flow immunoassay (LFI) for the detection of total malachite green (MG) residues in fish tissues. LFI was based on a colloidal gold nanoparticle-labeled monoclonal antibody against MG , which was generated by fast immunization of carboxymalachite green-bovine serum albumin. The half-maximum inhibition concentration (IC50) of MG monoclonal antibody (MG mAb) was 0.057 ng/ml with a linear range of 0.020–0.162 ng/ml. Following the optimization of LFI, a qualitatively visual limit of detection of 1 ng/ml and a semi-quantitatively limit of detection of 0.418 ng/ml (IC50 of the calibration curve) were obtained for total MG residues in 8 min. Cross-reactivity with most MG analogs was negligible, expect with crystal violet (CV; 78.6% cross-reactivity). LFI is a novel and sensitive assay based on MG mAb that has applications for the rapid detection of total MG and CV in fish products.
研究领域:
食品安全
·
免疫学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yu Lu,Lujia Sun,Cheng Li,Xiaoqin Wang,Wenqing Li,Tong Zhao,Linjuan Huang,Zhongfu Wang
Extensive cross-allergic reactivity exists among food-borne plants. As most allergens are glycoproteins, protein sensitization has been extensively studied. However, little attention has been given to the glycan component of glycoproteins. Here, we qualitatively and quantitatively compared the N -glycans of eight cross-reactive plant glycoproteins derived from peach pollen, soy, pine nut, cashew nut, pistachio, walnut, almond, and hazelnut. Cross-reactive carbohydrate determinants (CCDs) were widely present in the allergic glycoproteins according to the following order: pollen > soybean > pistachios > pine nuts > walnuts > cashews > hazelnuts > almonds. The N -glycan structure presented clear differences between the germplasm and vegetative tissue. Importantly, fucosylation and xylosylation levels correlated positively with extensive cross-reactivity between plants, which suggests that CCDs may be one of the causes of cross-allergy. This result provides the foundation for mechanistic studies on plant-derived CCDs as a source of food cross-allergies.
研究领域:
生物化学
·
免疫学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2014
·Vol. 21
·期 9
·5803-5809
·
IF 2.757
Li Ming,Sheng Enze,Yuan Yulong,Liu Xiaofeng,Hua Xiude,Wang Minghua
Europium (Eu 3+ )-labeled antibody was used as a fluorescent label to develop a highly sensitive time-resolved fluoroimmunoassay (TRFIA) for determination of clothianidin residues in agricultural samples. Toward this goal, the Eu 3+ -labeled polyclonal antibody and goat anti-rabbit antibody were prepared for developing and evaluating direct competitive TRFIA (dc-TRFIA) and indirect competitive TRFIA (ic-TRFIA). Under optimal conditions, the half-maximal inhibition concentration (IC 50 ) and the limit of detection (LOD, IC 10 ) of clothianidin were 9.20 and 0.0909 μg/L for the dc-TRFIA and 2.07 and 0.0220 μg/L for the ic-TRFIA, respectively. The ic-TRFIA has no obvious cross-reactivity with the analogues of clothianidin except for dinotefuran. The average recoveries of clothianidin from spiked water, soil, cabbage, and rice samples were estimated to range from 74.1 to 115.9 %, with relative standard deviations of 3.3 to 11.7 %. The results of TRFIA for the blind samples were largely consistent with gas chromatography ( R
2 = 0.9902). The optimized ic-TRFIA might become a sensitive and satisfactory analytical method for the quantitative monitoring of clothianidin residues in agricultural samples.
研究领域:
农业科学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2015
·Vol. 22
·期 19
·14882-14890
·
IF 2.828
Shi Haiyan,Sheng Enze,Feng Lu,Zhou Liangliang,Hua Xiude,Wang Minghua
A highly sensitive direct dual-labeled time-resolved fluoroimmunoassay (TRFIA) to detect parathion and imidacloprid simultaneously in food and environmental matrices was developed. Europium (Eu 3+ ) and samarium (Sm 3+ ) were used as fluorescent labels by coupling separately with L 1 -Ab and A 1 P 1 -Ab. Under optimal assay conditions, the half-maximal inhibition concentration (IC 50 ) and limit of detection (LOD, IC 10 ) were 10.87 and 0.025 μg/L for parathion and 7.08 and 0.028 μg/L for imidacloprid, respectively. The cross-reactivities (CR) were negligible except for methyl-parathion (42.4 %) and imidaclothiz (103.4 %). The average recoveries of imidacloprid ranged from 78.9 to 104.2 % in water, soil, rice, tomato, and Chinese cabbage with a relative standard deviation (RSD) of 2.4 to 11.6 %, and those of parathion were from 81.5 to 110.9 % with the RSD of 3.2 to 10.5 %. The results of TRFIA for the authentic samples were validated by comparison with gas chromatography (GC) analyses, and satisfactory correlations (parathion: R
2 = 0.9918; imidacloprid: R
2 = 0.9908) were obtained. The results indicate that the dual-labeled TRFIA is convenient and reliable to detect parathion and imidacloprid simultaneously in food and environmental matrices.
研究领域:
食品安全检测
·
环境监测
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2015
·
IF 2.912
Xiping Cui,Panpan Wu,Dan Lai,Shengwu Zheng,Yingshan Chen,Sergei A. Eremin,Wei Peng,Suqing Zhao
The diisobutyl phthalate (DiBP) hapten containing an amino group was synthesized successfully, and the polyclonal antibody against 4-amino phthalate–bovine serum albumin (BSA) was developed. On the basis of the polyclonal antibody, a rapid and sensitive indirect competitive fluorescence immunoassay (icFIA) has been established to detect DiBP in edible oil samples for the first time. Under the optimized conditions, the quantitative working range of the icFIA was from 10.47 to 357.06 ng/mL (R2 = 0.991), exhibiting a detection limit of 5.82 ng/mL. In this assay, the specific results showed that other similar phthalates did not significantly interfere with the analysis, with the cross-reactivity less than 1.5%, except for that of DiBAP. Thereafter, DiBP contamination in edible oil samples was detected by icFIA, with the recovery being from 79 to 103%. Furthermore, the reliability of icFIA was validated by gas chromatography–mass spectrometry (GC–MS). Therefore, the developed icFIA is suitable for monitoring DiBP in some edible oil samples.
研究领域:
食品科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CYTOTECHNOLOGY
·
2011
·Vol. 64
·期 2
·157-163
·
IF 1.277
Yi Yu,Wang Zhuhuan,Li Min,Zhu Keyin,Ying Guoqing
Monoclonal antibodies (McAbs) against chloramphenicol (CAP) were produced to detect CAP residues, which could be toxic and possesses a potential threat to human health. The CAP-BSA conjugate was obtained by bovine serum albumin (BSA) coupled with CAP, and used to immunize the mice. The splenocytes from the immunized mice were fused with mouse myeloma cells SP2/0 to form hybridoma, which may secrete McAbs against CAP. Hybridomas 1D 1 and 3G 12 secreting McAbs against CAP were obtained by screening. Ascites containing McAbs were prepared by injecting 1 x 10 6 cells of hybridoma 1D 1 and 3G 12 into the abdomen of mice. Protein A affinity chromatography was used to purify McAbs against CAP in a single chromatographic step with recovery yield above 80% and purity above 95% and full recovery of antibody activity. Experiments showed that McAb 3G 12 was highly specific for CAP and had no cross-reactivity with analogues which have a structure similar to CAP. The IC 50 value was 50.8 ng/mL.
研究领域:
免疫学
·
药理学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2017
·
IF 1.392
Joel Isanga,Daniel Mukunzi,Yanni Chen,Steven Suryoprabowo,Liqiang Liu,Hua Kuang,Chuanlai Xu
A monoclonal antibody (MAb) was produced against paromomycin and used in the development of an immunoassay and a lateral flow strip test for the detection of paromomycin residues in food matrices. The MAb had 25.1% cross-reactivity with neomycin, but was insensitive to other aminoglycosides. Tested under optimized conditions in 0.01 M phosphate-buffered saline, the half maximum inhibitory concentration (IC50) of the MAb was 0.61 ng/ml for paromomycin and 2.43 ng/ml for neomycin, with results obtained within 90 min. The mean recoveries from spiked food matrices were within the range of 64.56–105.85% for paromomycin and 54.08–100.55% for neomycin. The strip test results for different food matrices were obtained within 5 min and showed visual detection limits of 2.5–20 ng/ml (µg/kg) for paromomycin and 10–30 ng/ml for neomycin. Therefore, the developed immunoassay and strip test can be used in food analysis for routinely monitoring not only paromomycin but also neomycin residues.
研究领域:
食品科学
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Lin Zhang,Siqi Wu,Junfeng Liu,Meiling Ping,Weijuan Yang,FengFu Fu
Sulfonamides (SAs) is a class of antibiotics that extensively used for treating infectious diseases in livestock industries and aquaculture. Thus, it is urgent need to obtain the bio-receptor, which has excellent cross-reactivity and specificity to SAs, for developing high-throughput methods for the determination of multiple SAs even all commonly-used SAs, to realize the quick screening/detection of total SAs in animal-derived foods. We herein isolated several SAs-specific cross-reactive aptamers by using a library-immobilized SELEX with multi-SAs parallel selection strategy. Two of the isolated aptamers (Sul-01 and Sul-04) can specifically recognize and bind seven SAs respectively with higher binding affinity and no interference of non-sulfonamide antibiotics, and thus can be applied as bio-receptors for developing high-throughput aptasensors for the quick screening/detection of multiple SAs. By using the mixture of Sul-01 and Sul-04 as bio-receptor, a ratiometric fluorescent aptasensor was created for the quick detection of nine SAs including sulfamethoxydiazine (SMD), sulfapyridine (SPD), sulfaquinoxaline (SQ), sulfathiazole (ST), sulfamonomethoxine (SMM), sulfamerazine (SMR), sulfaguanidine (SG), sulfamethazine (SMZ) and sulfadiazine (SD) with a detection limit (LOD) of 0.10–0.50 μM, or total of above nine SAs with a LOD of 0.20 μM. The fluorescent aptasensor was successfully applied to detect each or total of SMD, SPD, SQ, ST, SMM, SMR, SG, SMZ and SD in fish samples with a recovery of 83 %–92 % and a relative standard deviation (RSD, n = 5) < 5 %. This study not only provided several promising bio-receptors for the development of diverse high-throughput aptasensors to achieve the quick screening of multiple SAs residues, but also provided a simple, stable and sensitive method for the quick screening of SMD, SPD, SQ, ST, SMM, SMR, SG, SMZ and SD in seafood.
研究领域:
分析化学
·
生物技术
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2015
·
IF 1.03
Xi Tian,Yaqing Dong,Yufen Wang,Zhaorui Song,Meng Meng,Sergei A. Eremin,Chuan Deng,Yongmei Yin,Rimo Xi
A rapid and homogenous fluorescence polarization immunoassay (FPIA) for diethyl phthalate is reported. Two haptens and three immunogens for diethyl phthalate were synthesized to generate polyclonal antibodies and fluorescein isothiocyanate labeled diethyl phthalate was prepared as the tracer. The antibodies, methanol concentration, and incubation time were optimized. The cross-reactivities of the optimized antibodies to other phthalate esters were characterized. The antibody shows excellent sensitivity with a limit of detection of 6.0 ng mL−1 and a half-inhibition concentration of diethyl phthalate of 40.4 ng mL−1. The immunoassay had a linear dynamic range from 10 to 200 ng mL−1. The mean coefficient of variation was lower than 11.3% for intra-assay and lower than 14.6% for inter-assay measurements. The limit of detection was 1.46 ng mL−1 in bottled drinking water, 0.34 µg mL−1 in juice, and 50.0 µg g−1 in pharmaceutical capsules. The recovery was from 85.9 to 114.9%, and the coefficients of variation were 4.3 to 17.0%. The developed immunoassay provided good sensitivity, specificity, accuracy, and may serve as an alternative for diethyl phthalate determination for field screening of food, pharmaceuticals, and environmental materials.
研究领域:
分析化学
·
环境科学
·
食品科学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Jiao Li,Yuan Ding,He Chen,Wanlin Sun,Yue Huang,Fengquan Liu,Minghua Wang,Xiude Hua
Propiconazole is a widely used fungicide in agricultural production, but it poses risks to human health. Here, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a monoclonal antibody (mAb) was established to detect propiconazole residues. Three haptens of propiconazole were designed and synthesized to prepare antigens. Ten mAbs were obtained using hapten B (5-(4-(2-((1H-1,2,4-triazol-1-yl)methyl)-4-propyl-1,3-dioxolan-2-yl)-3-chlorophenyl) pentanoic acid). The ic-ELISA based on the combination of mAb 2G2E12 and hapten B-ovalbumin (coating antigen) showed the highest sensitivity. After optimization, the 50% inhibition concentration, limit of detection and linear range of the ic-ELISA were 2.33 μg/L, 0.26 μg/L and 0.26–21.28 μg/L, respectively. The cross-reactivities for the analogs of propiconazole were ≤1.9%. The average recoveries of the spiked samples were 75.7%–117.0% with relative standard deviations of ≤9.4%. The results of the ic-ELISA were consistent with those of gas chromatography-mass spectrometry in the analysis of blind samples. Therefore, we provide an alternative tool for the accurate and reliable determination of propiconazole.
研究领域:
农业化学
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）